• Title, Summary, Keyword: Honey

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Acacia Honey Exerts Anti-Inflammatory Activity through Inhibition of NF-κB and MAPK/ATF2 Signaling Pathway in LPS-Stimulated RAW264.7 Cells

  • Kim, Ha Na;Park, Su Bin;Kim, Jeong Dong;Jeong, Hyung Jin;Jeong, Jin Boo
    • Proceedings of the Plant Resources Society of Korea Conference
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    • pp.97-97
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    • 2018
  • Honey used as conventional medicine has various pharmacological properties. In the honey and anti-inflammatory effect, Gelam honey and Manuka honey has been reported to exert anti-inflammatory activity. However, the anti-inflammatory effect and potential mechanisms of acacia honey (AH) are not well understood. In this study, we investigated anti-inflammatory activity and mechanism of action of AH in LPS-stimulated RAW264.7 cells. AH attenuated NO production through inhibition of iNOS expression in LPS-stimulated RAW264.7 cells. AH also decreased the expressions of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ as pro-inflammatory cytokines, and MCP-1 expression as a pro-inflammatory chemokine. In the elucidation of the molecular mechanisms, AH decreased LPS-mediated $I{\kappa}B-{\alpha}$ degradation and subsequent nuclear accumulation of p65, which resulted in the inhibition of $NF-{\kappa}B$ activation in RAW264.7 cells. AH dose-dependently suppressed LPS-mediated phosphorylation of ERK1/2 and p38 in RAW264.7 cells. In addition, AH significantly inhibited ATF2 phosphorylation and nuclear accumulation of ATF2 in LPS-stimulated RAW264.7 cells. These results suggest that AH has an anti-inflammatory effect, inhibiting the production of pro-inflammatory mediators such as NO, iNOS, $TNF-{\alpha}$, IL-6, $IL-1{\beta}$ and MCP-1 via interruption of the $NF-{\kappa}B$ and MAPK/ATF2 signaling pathways.

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Studies on Nosema Disease of Honey Bees 2. Effectiveness of Thimerosal to Control Growth of Nosema apis(Zander, 1909) in Rearing Honey Bees (꿀벌의 Nosema병에 관한 연구 2. 인공감염봉군에 대한 실험실내 치료시험)

  • Suh, Myung Deuk;Kang, Yung Bai;Kim, Chang Sup;Kim, Dong Sung
    • Korean Journal of Veterinary Research
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    • v.16 no.2
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    • pp.165-171
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    • 1976
  • Experimental approaches on the effectiveness of thimerosal to control growth of Nosema apis (Zander, 1909) were carried out in the rearing honey bees. The rearing honey bees were artificially infected with various levels of spore isolated from local honey bees. The results obtained were summarized as follows: 1. In the experiments of therapeutic chemicals for Nosema disease, 0.01% and 0.02% thimerosal of sucrose-honey mixture was the most effective agent but the each concentration of amprolium, furazolidone, hygiene, sulfadimethoxine and terramycin did not show the any effects 2. It showed very high therapeutic effectiveness (over than 90%) that the treatment of three times every other day after the treatment of three times consecutive every day with 0.01% thimerosal, or the treatment of three times consecutive every day with 0.02% thimerosal. 3. When 0.02% thimerosal was administered three times consecutive every day to honey bees at the 4th day before artificial inoculation of N. apis, it showed very high degree (100%) of prevalence control effectiveness, and it also showed high degree (over than 90%) in administration at the 7th day before, and over than 80% at the 10th day before. Then authors found that thimerosal has the prevalence control effectiveness as well as the treatment effectiveness. 4. In the rearing honey bee colony, 0.02% thimerosal showed the high degree (over than 80%) of therapeutic effectiveness with the various levels which contained from the light decree of infection to the severe degree of it.

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Quality Characteristics of White Pan Bread with Honey (꿀을 첨가한 식빵의 품질특성)

  • Kim, Eun-Ji;Lee, Kwang-Suck
    • Culinary science and hospitality research
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    • v.19 no.4
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    • pp.147-160
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    • 2013
  • This research investigates the quality characteristics of bread added with honey. The overall effects of honey on white pan bread were examined in terms of mixograph, fermentation rates, pH levels, TPA, colorimeter, water activity, hardness and moisture content of bread through different storage periods(1, 2, 3 days) using sensory evaluation. According to mixograph, all the samples were found to be proper between 3 and 5 minutes of peak time and their peak values were at the level of 60%. Also, HL100 had the highest fermentation rate and fermentation persistence from 60 min to 135 min. HL100 showed the lowest pH level in ingredient, dough, fermentation and crumb, and the highest specific volume. TPA analysis showed that HL100 had the lowest hardness and the highest springiness of all. And HL100 was the lowest in hardness(i.e. highest in softness) and had the highest moisture content after storage of 72 hours. Preference test showed that HL100 got the best texture, flavor, mouth feel, taste and overall acceptance. In these results of experiment, honey affects the quality of dough and bread, and liquid honey could be used as a substitute for sugar in breadmaking.

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Analysis of important quality-related components in honeys collected in Incheon metropolitan area (인천지역에서 유통되는 벌꿀의 품질조사)

  • Lee, Sung-Mo;Hong, Jee-Young;Park, Eun-Jeong;Kim, Jung-Im
    • Korean Journal of Veterinary Service
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    • v.30 no.1
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    • pp.23-32
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    • 2007
  • A total of 60 samples (38 domestic honeys and 22 foreign honeys) were collected from October in 2003 to May in 2004 in Incheon metropolitan area, and contents of important quality-related components in honeys were analyzed using methods of Korea Food Code. Most of the honeys were satisfied with Korean limit (21%) in moisture content even though 4 samples brought by Korean travellers and 1 sample in domestic honey (wild flower) were higher than the limit. The lowest mean level of moisture in the honeys was imported foreign honeys (16.95%). All of the samples were below of 0.6% in the analysis of ash contents. The highest content was imported foreign honey (0.19%) while the lowest content was in domestic acacia honey (0.05%). Acidity of domestic honeys (9.49-9.94meq/kg) was approximately half of that in foreign honeys. All samples were satisfied with Korean limit (40.0meq/kg). In the analysis of fructose and glucose, only three samples of foreign honeys were lower than Korean limit (sum of both ; 65%). The content of sucrose in all samples was less than 7%. Mean of Hydroxymethylfurfural (HMF) contents were 12.83-24.3mg/kg in domestic honey, and 31.34-45.58mg/kg in foreign honey. However, three samples brought by Korean travellers were not satisfied with Korean limit (${\leq}80mg/kg$). In conclusion, quality of domestic honey was better than that of foreign honey. Also, it may be needed that the honeys purchased by Korean travellers in foreign countries should be continuously monitored.

Quality Characteristics of Various Honeys from Different Sources (밀원을 달리한 다양한 꿀의 품질 특성)

  • Jung, Mi-Ea;Lee, Si-Kyung
    • Food Science of Animal Resources
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    • v.28 no.3
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    • pp.263-268
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    • 2008
  • This study was carried out to investigate the quality characteristics of various honeys originating from different sources, and to identify factors potentially useful for distinguishing the honey sources. The sugar composition, moisture content and total acidity of twenty six honey samples were analyzed. The moisture contents were lowest in honeys from Acacia and were not greatly different with regard to honey sources. Fructose content was highest, followed by glucose, sucrose and maltose in all kinds of honey. Sugar composition did not show any reliable criterion useful for identifying the sources of honey samples. The fructose/glucose ratio did not differ among samples, however the ratios in honeys from Acacia and Chestnut were higher than honey from poly floral sources. The total acidity of honeys ranged 10.30-12.45 meq/kg regard-less of the source, thus acidity is not a useful criterion for identifying the honey source.

Characteristics of Wine Fermented with Fruit of Flowering Cherry and Honey (버찌와 꿀을 함께 발효한 버찌-꿀 술의 발효특성)

  • Jang, Ki-Hyo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.7
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    • pp.3103-3108
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    • 2011
  • This work was aimed to evaluate supplementation of honey as sole carbon source on the fermentation characteristics of wine fermented with fruit of flowering cherry and honey(flowering cherry-honey wine). Physiochemical changes of flowering cherry-honey wine(2 L) were investigated during 30 days in fermentation by strain of Saccharomyces bayanus (EC-118). At the beginning of fermentation, fructose was most abundant sugar then glucose and sucrose were followed. As fermentation proceeded, utilization of glucose by S. bayanus (EC-118) was faster than fructose, so that the ratio of fructose/glucose was increased. During fermentation for 30 days, pH and viable yeast count was changed rapidly between 0 to 5 days, while $^{\circ}Brix$(%) decreased gradually for 30 days. Final total titratable acidity, pH, $^{\circ}Brix$(%) and ethanol content of flowering cherry-honey wine were 0.43%, pH 3.5, $9.7^{\circ}Brix$(%) and 14%, respectively. Our finding demonstrate that flowering cherry-honey could be benefical supplements for wine production.

Anti-Inflammatory Activity of Acacia Honey through Inhibition of NF-κB and MAPK/ATF2 Signaling Pathway in LPS-Stimulated RAW264.7 Cells

  • Kim, Ha Na;Son, Kun Ho;Jeong, Hyung Jin;Park, Su Bin;Kim, Jeong Dong;Jeong, Jin Boo
    • Korean Journal of Plant Resources
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    • v.31 no.6
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    • pp.612-621
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    • 2018
  • Honey used as conventional medicine has various pharmacological properties. In the honey and anti-inflammatory effect, Gelam honey and Manuka honey has been reported to exert anti-inflammatory activity. However, the anti-inflammatory effect and potential mechanisms of acacia honey (AH) are not well understood. In this study, we investigated anti-inflammatory activity and mechanism of action of AH in LPS-stimulated RAW264.7 cells. AH attenuated NO production through inhibition of iNOS expression in LPS-stimulated RAW264.7 cells. AH also decreased the expressions of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ as pro-inflammatory cytokines, and MCP-1 expression as a pro-inflammatory chemokine. In the elucidation of the molecular mechanisms, AH decreased LPS-mediated $I{\kappa}B$-${\alpha}$ degradation and subsequent nuclear accumulation of p65, which resulted in the inhibition of $NF-{\kappa}B$ activation in RAW264.7 cells. AH dose-dependently suppressed LPS-mediated phosphorylation of ERK1/2 and p38 in RAW264.7 cells. In addition, AH significantly inhibited ATF2 phosphorylation and nuclear accumulation of ATF2 in LPS-stimulated RAW264.7 cells. These results suggest that AH has an anti-inflammatory effect, inhibiting the production of pro-inflammatory mediators such as NO, iNOS, $TNF-{\alpha}$, IL-6, $IL-1{\beta}$ and MCP-1 via interruption of the $NF-{\kappa}B$ and MAPK/ATF2 signaling pathways.

Determination and Monitoring of Grayanotoxins in Honey Using LC-MS/MS (LC-MS/MS를 이용한 벌꿀 중 grayanotoxin 분석법 연구 및 실태조사)

  • Lee, Sook-Yeon;Choi, Youn-Ju;Lee, Kang-Bong;Cho, Tae-Yong;Kim, Jin-Sook;Son, Young-Wook;Park, Jae-Seok;Im, Sung-Im;Choi, Hee-Jung;Lee, Dong-Ha
    • Korean Journal of Food Science and Technology
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    • v.40 no.1
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    • pp.8-14
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    • 2008
  • This study was performed to establish analysis methods, and evaluated for grayanotoxin in domestic/foreign honey and wild honey. The molecular weight of grayanotoxins I, II and III, excluding grayanotoxin III that has been commercialized, were analyzed by LC-MS/MS. Then, the molecular structure of grayanotoxins I and II were analyzed by NMR. A total 111 samples (25 Korean honey, 21 Korean wild honey, 13 Korean honeycomb honey, 44 foreign honey, 8 foreign wild honey) were examined to determined whether or not each sample contained grayanotoxins I, II, and III. The honey samples were mixed with methanol and loaded into a tC18 cartridge, the filtrate was diluted with water, and the mixture was then analyzed by ESI triple-quadrupole LC-MS/MS. Grayanotoxins were only found in the foreign wild honey and were not detected in Korean honey, Korean honeycomb honey, or Korean wild honey. Three of the samples contained grayanotoxin I, II, and III, and one sample contained only grayanotoxins I and III. The lowest level for grayanotoxin I was 3.13 ${\pm}$ 0.00 mg/kg, and the highest level was 12.93 ${\pm}$ 0.01 mg/kg. The levels of grayanotoxin II were 0.84 ${\pm}$ 0.01 mg/kg, 0.92 ${\pm}$ 0.00 mg/kg and 1.08 ${\pm}$ 0.01 mg/kg, respectively. The lowest level of grayanotoxin III was 0.25 ${\pm}$ 0.01 mg/kg and the highest level was 3.29 ${\pm}$ 0.74 mg/kg. Through this study, safety management for foreign wild honey has been enabled.

Study on Rheological Properties of Honey Cinseng Powder Mixtures. (벌꿀-인삼분 혼합물의 유동학적 성질에 관한 연구)

  • 김남희;정기용
    • YAKHAK HOEJI
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    • v.21 no.2
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    • pp.87-94
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    • 1977
  • The rheological properties of some honey-ginseng powder mixturs, used as pharmaceutical pills, were studied extensively by employing five kinds of honey, acacia, chestnut, buckwheat, green perilla and bush clover. Relation between the viscosity of the sample mixtures and the concentrations of ginseng powder and the temperature effect on the internal change of the structure were studied. The results obtained are as follows: 1) A complete gel formation of honey-ginseng mixtures was observed when about 50 days have passed after the sample preparation. 2) The viscosity of honey-ginseng powder mixtures was related with the concentration of ginseng powder, the temperature and the period of time required in gel formation as shown in the following equation. n=noexp$[{\kappa}_{1}c^{n1}+{\kappa}_{2}(T-273)^{-n2}+{\kappa}_{3} t^{n3}]$

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A Case Report of Saliva-type Hyperamylasemia in Mad Honey Poisoning (석청 섭취 후 고아밀라아제혈증이 합병된 1례)

  • Lee, Kun-Woo;Park, Kyu-Nam;Lee, Mi-Jin
    • Journal of The Korean Society of Clinical Toxicology
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    • v.4 no.2
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    • pp.166-169
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    • 2006
  • Poisonings caused by 'mad honey' are known to occur in response to grayanotoxins, which bind to sodium channels in the cell membrane, increasing membrane sodium permeability and preventing inactivation. Mild symptoms of mad honey intoxication are dizziness, weakness, hypersalivation, nausea, vomiting, and paresthesia. Severe intoxication, however, leads to serious cardiac manifestations such as atrioventricular block, dose-dependent hypotension, bradycardia, and respiratory depression. Atropine and vasoactive drugs improve symptoms of both bradycardia and respiratory rate depression. We report an unusual case of saliva-type hyperamylasemia in a mad honey poisoning patient who developed clinically significant bradycardia. She recovered fully within 3 days following atropine administration and medical treatment.

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