• Title, Summary, Keyword: Helicobacter pylori

Search Result 632, Processing Time 0.055 seconds

Studies on Food Components and Food Additives Affecting the Growth Patterns of Helicobacter pylori (Helicobacter pylori의 생육에 영향을 미치는 성분과 식품첨가물에 관한 연구)

  • 정혜진
    • The Korean Journal of Food And Nutrition
    • /
    • v.12 no.5
    • /
    • pp.470-477
    • /
    • 1999
  • The purpose of this study was to choose prinicipal food components contained in diet foods and food additives used for manufacturing processed foods and elucidate their in vivo effects on the growth pattern of Helicobacter pylori. To do this the antibacterial effects of various sources of carbon nitrogen and mineral as an effect agent on Helicobacter pylori were first assessed based upon bacterial growth degree. results show that the source of carbon tested had different effects on bacterial growth of Helicobacter pylori. It was revealed that a promotional effect of monosaccharides resulted in enhanced growth of Helicobacter pylori compared with disaccharides and polysacchrides, in particular glucose was observed to be most effective in growth of Helicobacter pylori among monosaccharides teste whereas mannose to hinder the growth of Helicobacter pylori. Polyols such as sorbitol mannitol maltitol and xylitol was however observed to show no promotion or suppression effect on growth of Helicobacter pylori. Apart from these the sources of amino acid and inorganic nitrogen were chosen and tested to assess the promotion or suppression effect of nitrogen sources on growth of helicobacter pylori. It was found that amino acid such as lysine showed its promotion effect on the growth of Helicobacter pylori while arginine (NH4)2SO4 and NH4Cl showed no effect on its growth. Ammoia and urea were however observed to have a positive effect on the growth of Helicobacter pylori. Among these effect agents lysine and methionine were revealed to show the most positive effect on growth of Helicobacter pylori. Minerals such as MgSO4 KH2PO4 and MgCl2 appered to exert their positive growth effects whereas CaCl2 and CaSo4 had a little effect. In addition FeSO4 FeCl2 and FeCl3 brought suppression on the growth of helicobacter pylori. In studies of the growth of Helicobacter pylori by food additives ascorbic acid showed extreme suppression on its growth,. Sodium nitrate and sodium chloride were also found to be of negative effect on the growth of Helicobacter pylori in rder of degree whereas tocopherol had nothing to do with microbial growth.

  • PDF

Survey on the Risk Factor Affecting on the Gastric Cancer Incident to the Healthy Adults in Rural Area -Infection of Helicobacter pylori in Whachon Area Kang-Won Do- (위암발증 원인에 대한 생활습관 및 영양섭취에 있어서 농촌지역 주민 조사 -강원도 화천군 주민의 Helicobacter pylori 감염률을 중심으로-)

  • 이정선
    • The Korean Journal of Food And Nutrition
    • /
    • v.12 no.1
    • /
    • pp.13-19
    • /
    • 1999
  • To examine the relationship between the casual infection of gastric cancer and lifestyle and nutritional status we surveyed 169 persons 90 females and 79 males aged 40∼69 years from June 7 to June 14 1997 in Whachon area Kang-Won Do Korea. For this purpose we investigated Helicobacter pylori infec-tion and identified the effect of sex age, smoking alcohol consumption economic status occupation life-style and food intake on Helicobacter phlori infection. Helicobacter pylori status was evaluated using an en-zyme-linked immunosorbant assay(ELISA) for anti-Helicobacter pylori immunoglobulin G(IgG). Helicob-acter pylori infection was present in 66.3% of total subjects namely 67.7% of female and 64.4% of male and its prevalence increased with smoking low education level and low monthly income. Energy total protein calcium and vitamin C intakes in the negative Helicobacgter phlori infection group were higher than those in positive Helicobacter pylori infection group. However iron and niacin intakes were lower in the helicobacter pylori negative group than in the positive group.

  • PDF

A Study on the Gastric diseases caused by Helicobacter Pylori (Helicobacter Pylori에 의해 유발(誘發)되는 위질환(胃疾患)에 대(對)한 고찰(考察))

  • Lee, Yeon Weol;Son, Chang Kyu;Cho, Jong Kwan
    • Journal of Haehwa Medicine
    • /
    • v.8 no.2
    • /
    • pp.153-170
    • /
    • 2000
  • This paper presents a study for a development of the oriental medical treatment on the gastric disease caused by Helicobacter Pylori. Helicobacter Pylori is observed in stomach mucosa and it is caused gastric disease via various routes. Helicobacter Pylori is spread generally mouth to mouth by men. So it is related to personal health condition or life style. The treatment of Helicobacter Pylori is an antibiotic combination therapies, but it caused a problem of side effect and drug resistant. In the oriental medical treatment, an immunopotentiation reduces an infective rate of Helicobacter Pylori and prevents a progressive gastric disease.

  • PDF

H. pylori Infection and Gastric Carcinogenesis (H. pylori Infection 감염과 위암 발생)

  • Han Sang-Uk;Cho Yong-Kwan;Chung Jae-Yun;Park Hyun-Jin;Kim Young-Bae;Nam Ki-Taek;Kim Dae-Yong;Joo Hee-Jae;Choi Jun-Hyuk;Kim Jin-Hong;Lee Ki-Myung;Kim Myung-Wook;Hahm Ki-Baik
    • Journal of Gastric Cancer
    • /
    • v.2 no.2
    • /
    • pp.73-80
    • /
    • 2002
  • In spite the fact that H. pylori infection might be the causative organisms of acute and chronic gastritis, peptic ulcer diseases and the definition as the class I carcinogen by WHO IARC, still debates exist about the relationship between H. pylori and gastric carcinogenesis. Epidemiological and animal studies demonstrated a link between gastric cancer and chronic infection with H, pylori, but the exact mechanism responsible for the development of gastric cancer in H. pylori-infected patients still remain obscure. In order to declare the clear association, definate evidences like that decrement in the incidence of gastric cancer after the eradication of H. pylori in designated area compared to noneradicated region or the blockade of specific mechanism acting on the carcinogenesis by H. pylori infection. The other way is to identify the upregulating oncogenes or downregulating tumor suppressor genes specifically invovled in H. pylori-associated carcinogenesis. For that, we established the animal models using C57BL/6 mice strain. Already gastric carcinogenesis was developed in Mongolian gerbils infected with H. pylori, but there has been no development of gastric cancer in mice model infected with H. pylori after long-term evaluation. Significant changes such as atrophic gastritis were observed in mice model. However, we could observe the development of mucosal carcinoma in the stomach of transgenic mice featuring the loss of TGF-beta sig naling by the expressions of dominant negative forms of type II receptor specifically in the stomach. Moreover, the incidence of gastric adenocarcinoma was significantly increased in group administered with both MNU and H. pylori infection than MNU alone, signifying that H. pylori promoted the gastric carcinogenesis and there might be host susceptibility genes in H. pylori-associated gastric carcinogenesis. Based on the assumption that chronic, uncontrolled inflammation might predispose to carcinogenesis, there have been several evidences showing chronic atrophic gastritis predisposed to gastric carcinogenesis in H. pylori infection. Although definite outcome of chemoprevention was not drawn after the longterm administration of anti-inflammatory drug in H. pylori infection, the actual incidence of atrophic gastritis and molecular evidence of chemoprevention could be obtained. Selective COX-2 inhibitor was effective in decreasing the development of gastric carcinogenesis provoked by H. pylori infection and carcinogen like in chemoprevention of colon carcinogenesis.

  • PDF

Study on the antimicrobial activities of herbal extracts against Helicobacter pylori (Helicobacter pylori에 대한 항균활성을 가진 천연물의 검색과 항균력 비교 연구)

  • Lee, Hyun-A;Kim, Okjin
    • Korean Journal of Veterinary Research
    • /
    • v.53 no.2
    • /
    • pp.117-123
    • /
    • 2013
  • Infection with Helicobacter (H.) pylori is strongly associated with duodenal and gastric ulcers. Substantial epidemiological data has revealed that high rates of H. pylori infection might be related to high rates of gastric cancer. In this study, a medicinal herbal extracts were examined and screened for anti-H. pylori activity. Seventy percent ethanol was used for herbal extraction. For anti-H. pylori activity study, the inhibitory zone tests with 74 herbal compounds were conducted. As the results, thirteen compounds including Cinnamomi Cortex, Magnoliae Cortex and Meliae Fructus were revealed strong anti-H. pylori activities.

Anti-Helicobacter and Anti-inflammatory Effects of Sohamhyungtang in Helicobacter pylori-Infected Human Gastric Epithelial AGS cells

  • Won, SangBum;Yim, Dongsool;Choi, SungSook
    • Natural Product Sciences
    • /
    • v.23 no.3
    • /
    • pp.175-182
    • /
    • 2017
  • This study evaluated the anti-Helicobacter and anti-inflammatory effects of Sohamhyungtang (SHHT). The minimum inhibitory concentration (MIC) of SHHT against Helicobacter pylori (H. pylori) was determined by the agar dilution method. Expression of the H. pylori cagA gene in the presence of SHHT was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Inhibition of H. pylori urease by SHHT was determined by the phenol-hypochlorite assay. Antiadhesion activity of SHHT was measured by urea-phenol red reagent. Inhibition of nitric oxide (NO) production in AGS cells was measured with Griess reagent. Inducible nitric oxide synthase (iNOS) and IL-8 mRNA expression in AGS cells which were infected with H. pylori was determined by qRT-PCR. IL-8 level was measured by enzyme-linked immunosorbent assay (ELISA). The MIC of SHHT was $100{\mu}g/mL$ and the expression of cagA gene was decreased about 25 folds in the presence of SHHT. H. pylori urease was inhibited 90% by SHHT. SHHT inhibited H. pylori adhesion on AGS cell in a concentration dependent manner. mRNA expression of iNOS and IL-8 and the production of NO and IL-8 were significantly decreased in the presence of SHHT. In conclusion, SHHT showed anti-Helicobacter activity and has potent anti-inflammatory effect on H. pylori-induced inflammation in human gastric epithelial AGS cells.

Effects of Anti-Helicobacter pylori IgY Powder to Protect Mice from Helicobacter pylori (Helicobacter pylori 감염생쥐에서 항-Helicobacter pylori 난황항체 분말의 효과)

  • Jung, Soon-Hee;Kim, Hyun-Jue;Lyoo, Young-Soo;Rho, Jeong-Hae;Lee, Nam-Hyung
    • Korean Journal of Food Science and Technology
    • /
    • v.38 no.1
    • /
    • pp.93-98
    • /
    • 2006
  • Effects of anti-Helicobacter pylori IgY powder on H. pylori infection were evaluated 3 and 7 weeks after powder feeding by urease, PCR, and histological tests, and specific IgG assay of murine gastric tissue using mouse model. To produce anti-H. pylori IgY powder, laying hens were immunized with H. pylori prior to egg yolk harvest. C57BL/6 mice showing high response to H. pylori were infected with H. pylori and fed with the anti-H. pylori IgY powder. In urease and PCR tests, urease activity and gene count of anti-H. pylori IgY powder-fed group significantly decreased in comparison with control. Histological results indicated anti-H. pylori IgY powder effectively protected mice from H. pylori.

Helicobacter Pylori Associated Gastritis Increases Risk of Colorectal Polyps: a Hospital Based-Cross-Sectional Study in Nakhon Ratchasima Province, Northeastern Thailand

  • Tongtawee, Taweesak;Kaewpitoon, Soraya;Kaewpitoon, Natthawut;Dechsukhum, Chavaboon;Leeanansaksiri, Wilairat;Loyd, Ryan A;Matrakool, Likit;Panpimanmas, Sukij
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.17 no.1
    • /
    • pp.341-345
    • /
    • 2016
  • Background: Colorectal polyps are common in Thailand, particularly in the northeastern region. The present study aimed to determine any correlation between Helicobacter pylori-associated gastritis and colorectal polyps in the Thai population. Materials and Methods: A total of 303 patients undergoing esophagogastroduodenoscopy with colonoscopy for investigation of chronic abdominal pain participated in this study from November 2014 to October 2015. A diagnosis of Helicobacter pylori associated gastritis was made if the bacteria were seen on histopathological examination and a rapid urease test was positive. Colorectal polyps were confirmed by histological examination of colorectal biopsies. Patient demographic data were analyzed for correlations. Results: The prevalence of colorectal polyps was 77 (25.4%), lesions being found more frequently in Helicobacter pylori infected patients than non-infected subjects [38.4% vs. 12.5%; Odds Ratio (OR) (95% CI): 2.26 (1.32 - 3.86), p < 0.01]. Patients with Helicobacter pylori - associated gastritis were at high risk of having adenomas featuring dysplasia [OR (95% CI): 1.15 (1.16 - 7.99); P = 0.02]. There was no varaition in location of polyps, age group, sex and gastric lesions with respect to Helicobacter pylori status. Conclusions: This study showed that Helicobacter pylori associated gastritis is associated with an increased risk of colorectal polyps, especially adenomas with dysplasia in the Thai population. Patients with Helicobacter pylori-associated gastritis may benefit from concurrent colonoscopy for diagnosis of colorectal polyps as a preventive and early treatment for colorectal cancer.

Anti-bacterial and Anti-inflammatory Effects of Angelica dahurica Extracts in Helicobacter pylori-infected Human Gastric Epithelial AGS Cells (백지(Angelica dahurica) 추출물의 Helicobacter pylori에 대한 항균력 및 H. pylori로 유도한 염증반응에 대한 항염 효과)

  • Choi, Min Kyeong;Yim, Dongsool;Choi, SungSook
    • Korean Journal of Pharmacognosy
    • /
    • v.49 no.3
    • /
    • pp.255-261
    • /
    • 2018
  • The aim of this study was to evaluate the anti-helicobacter activity and anti-inflammatory activity of Angelica dahurica (AD). The minimum inhibitory concentration(MIC) of AD against Helicobacter pylori(H. pylori), expression of the H. pylori cagA gene in the presence of AD was determined. Inhibition of H. pylori urease by AD, inhibition of nitric oxide (NO) production in AGS cells was measured. IL-8 mRNA expression in AGS cells which were infected with H. pylori and IL-8 level was measured. The MIC of MeOH Ex. of AD was $250{\mu}g/mL$ and the expression of cagA gene was decreased about 88% in the presence of AD. The activity of H. pylori urease was inhibited 70% by AD. mRNA expression of IL-8 and the production of NO and IL-8 were significantly decreased in the presence of AD. In conclusion, AD showed anti-Helicobacter activity and has potent anti-inflammatory effect on H. pylori-induced inflammation in human gastric epithelial AGS cells.