• Title, Summary, Keyword: Hatching

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Studies on the deacidification times of artificial hatching silkworm eggs (인공부화잠종 탈산시간의 장단이 부화율에 미치는 영향)

  • 김윤식
    • Journal of Sericultural and Entomological Science
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    • v.10
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    • pp.57-58
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    • 1969
  • Artificial silkworm eggs must do washing the acid completely after soaking HCl. Author experimented following deacidification times: 30 minutes, 1 hour, 2 hours, 6 hours soaking and 12 hours soaking eggs in the fresh water after artificial hatching, and incubated room temperature. There was no difference 1 hour's with control but more than 1 hour deacidification influenced badly in hatching ratio. Especially above than 6 hours deacidification was not only delaying 1 day in hatching date but also decreasing in practical hatching ratio.

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Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice

  • Lee, Young Seok;Park, Min Jung;Park, Sea Hee;Koo, Ja Seong;Moon, Hwa Sook;Joo, Bo Sun
    • Clinical and Experimental Reproductive Medicine
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    • v.42 no.2
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    • pp.51-57
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    • 2015
  • Objective: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age. Methods: Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of $15-20{\mu}m$. Results: In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones. Conclusion: These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

Texture-based Hatching for Color Image and Video

  • Yang, Hee-Kyung;Min, Kyung-Ha
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.5 no.4
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    • pp.763-781
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    • 2011
  • We present a texture-based hatching technique for color images and video. Whereas existing approaches produce monochrome hatching effects in considering of triangular mesh models by applying strokes of uniform size, our scheme produces color hatching effects from photographs and video using strokes with a range of sizes. We use a Delaunay triangulation to create a mesh of triangles with sizes that reflect the structure of an input image. At each vertex of this triangulation, the flow of the image is analyzed and a hatching texture is then created with the same alignment, based on real pencil strokes. This texture is given a modified version of a color sampled from the image, and then it is used to fill all the triangles adjoining the vertex. The three hatching textures that accumulate in each triangle are averaged and the result of this process across all the triangles forms the output image. We can also add a paper texture effect and enhance feature lines in the image. Our algorithm can also be applied to video. The results are visually pleasing hatching effects similar to those seen in color pencil drawings and oil paintings.

Effects of Temperature and Salinity on Hatching Rate of Fertilized Egg in Fleshy Shrimp, Penaeus chinensis (대하(Penaeus chinensis) 수정난의 부화에 미치는 수온과 염분의 영향)

  • HUR Sung Bum;KIM Hyun Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.1
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    • pp.1-8
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    • 1996
  • The effects of temperature and salinity on hatching rate of fertilized egg were examined to develop the method for the efficient seed production in fleshy shrimp, Penaeus chinensis. At the temperature ranging from $20^{\circ}C\;to\;24^{\circ}C$, the hatching rate increased with temperature and the highest hatching rate $(95.5\%)$ occurred at $24^{\circ}C$. But, with the temperature above $26^{\circ}C$, the hatching rate decreased suddenly, and at $30^{\circ}C$, the egg did not hatch at all. The hatching rate increased with salinity within the range of $20\%_{\circ}\;to\;31\%_{\circ}$ and the highest hatching rate $(95.5\%)$ occurred at $31\%_{\circ}$. However, with the salinity above $31\%_{\circ}$, the rate decreased abruptly, and it was only $25.5\%\;at\;40\%_{\circ}$. The hatching speed of egg was the fastest at $24^{\circ}C$. The hatching rate at $24^{\circ}C$ with the salinity of $31\%_{\circ}\;was\;30.5\%$ within 18hrs of the hatching experiment and it attained to $95.5\%$ within 48hrs of the hatching experiment. At $24^{\circ}C$, the hatching rate with the salinity of $30\~31\%_{\circ}$ is 1,2 times higher than that of egg with the salinity of $33\~35\%_{\circ}$.

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Shadow Techniques in Real-time Hatching Rendering (실시간 해칭 렌더링에서 그림자 기법)

  • Kim, Chan-Soo;Kim, Dae-Myung
    • Proceedings of the Korea Contents Association Conference
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    • pp.806-810
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    • 2006
  • The research of computer graphics is divided into two parts of photorealistic rendering and non-photorealistic rendering. The purpose of non-photo realistic rendering is to make image like cartoon, water-color, hatching etc. In this paper, we study for real-time hatching rendering and shadow techniques and we combine two techniques to make real-time hatching shadow. In shadow techniques we apply projected texture shadow to hatching rendering. Eventually, we introduce natural real-time hatching shadow through comparison and analysis.

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Real-time Image-space Hatching (실시간 영상 공간 해칭 -GPU 기반 실시간 픽셀 단위 영상공간 해칭-)

  • Kim, Yong-Jin;Lee, Seung-Yong
    • 한국HCI학회:학술대회논문집
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    • pp.459-462
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    • 2009
  • Hatching is an effective artistic tool for conveying shape and shading by placing parallel line strokes on drawing objects. We present a simple and effective per-pixel image-space hatching method to draw line strokes using given stroke directions. Our hatching method directly runs on the screen and it can efficiently render highly complex scenes in hatching styles. We implement the algorithm using a pixel shader in a modern GPU.

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Concanavalin A Mediated Calcium Changes on Expansion and Hatching of the Mouse Blastocyst (Concanavalin A를 매개로한 세포내 Calcium의 변화와 생쥐 포배의 팽창과 부화)

  • Cheon, Yong Pil
    • Clinical and Experimental Reproductive Medicine
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    • v.32 no.4
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    • pp.337-346
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    • 2005
  • Objective: The oligosaccharide moieties of glycoproteins and proteoglycans have a vital function in blastocyst differentiation. Concanavalin (ConA), a lectin, is known to bind on the preimplantation embryos, especially on blastocyst. In this study, we investigated whether ConA can modulate the trophoblast development and about the regulating mediator. Also, we investigated whether expansion is enough for hatching procession of the mouse blastocyst. Method: Embryos were collected at 72 h post hCG injection and chemicals were treated after 24 h (96 hr post hCG injection). ConA or calcium ionophore A23187 were exposed to blastocyst and than analysis the developmental process for 48 hr. Intracellular free-$Ca^{2+}$ concentration in trophectoderm was measured with confocal laser microscope after exposing to ConA or calcium ionophore A23187. ConA-pretreated blastocyst exposed to the calcium ionophore A23187 and then analyzed the developmental process. Otherwise ouabain was treated to the blastocyst to block the $Na^+/K^+$-ATPase activity. Results: In contrast to the control blastocyst, the ConA-exposed blastocysts developed beyond the expansion stage with significantly high rate (90.4%) at 12 h post administration. ConA induced an increase the intracellular $Ca^{2+}$ concentration in trophectoderm. Calcium ionophore A23187 also stimulated expansion of blastocyst. Most of the control blastocysts developed to the hatching stage at 144 h post hCG injection. However, strongly 65% of the ConA-exposed embryos were arrested at expanded stage at same time point. The developmental progression rates to hatching stage of both ConA- and calcium ionophore A23187-expose blastocysts were significantly lower than that of the control. However ConA-pretreated embryos developed to the hatching stage like control embryos. Ouabain showed a tendency to delayed the progress to expansion stage but did not inhibit the development to the hatching stage. Conclusion: ConA-mediated expansion is the result of the increase of intracellular free-calcium in blastocyst stage embryo. It is suspected that expansion of the blasocyst is a essential indirect factor in hatching and the calcium may triggering the cellular mechanisms for the both expansion and hatching progression.

Seedling Production and Rearing of Pale Chup, Zacco platypus (Temminck et Schlegel) (피라미, Zacco platypus (Temminck et Schlegel)의 종묘생산)

  • 남명모;최낙중;김성원;석규진;이종윤
    • Journal of Aquaculture
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    • v.12 no.1
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    • pp.25-30
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    • 1999
  • This study was carried out to produce the seedling of the larvae and juveniles of pale chup, Zacco platypus (Cyprinidae) in terms of artificial hatching, feeding behavior and growth rates. The total lengths of the newly hatched larvae were 7.6~8.2mm (mean: 7.97mm). The hatched larvae rose to the surface 3 days after hatching. The larvae were fed Daphnia, rotifer, Artemia and powdered feed. Hatching experiments were conducted in jar incubators under the different water temperature conditions, $20~32^{\circ}C$. The highest hatching rate was at $26^{\circ}C$ after 47 hours. Jar incubators was the highest hatching rate (89%), and cage and Californian incubators were useful and relatively high hatching rates (>89%). The size of the larvae 50 days after hatching were 30.0mm (25.6~32.0mm). As the water temperatures was increased, the growth rate of the larvae was also increased. Especially, the growth rates were favorable over $26^{\circ}C$, but survival was the worst at $32^{\circ}C$. This species was quick to accept assorted feed after hatching. The assorted feeds for flounder and rockfish better than that of carp in reference to growth rate.

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The Hatching Rate of Resting Eggs of the Rotifer Brachionus plicatilis according to Preservation Method (보관 방법에 따른 Rotifer Brachionus plicatilis 내구란의 부화)

  • Youn, Joo-Yeon;Hur, Sung-Bum
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.6
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    • pp.665-670
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    • 2011
  • The rotifer Brachionus plicatilis is one of the most important food organisms in aquaculture. The resting eggs produced by mictic female rotifers are easily stored and hatched, making them useful as the starter for the mass culture of rotifers in marine larval culture. This study examined the optimum preservation method for resting eggs to ensure a high hatching rate. To produce resting eggs, the marine rotifer B. plicatilis was cultured with Nannochloris oculata (KMMCC 16). The resting eggs were harvested and cryopreserved using 5% and 10% methanol (MeOH), dimethylsulfoxide (DMSO), and glycerol as cryoprotectant agents (CPAs). The cryopreservation comprised slow or rapid freezing and the resting eggs were stored for one month in liquid nitrogen ($-196^{\circ}C$). The resting eggs were also dried at different temperatures (30, 40, and $50^{\circ}C$) and for different times (1, 2, and 3 h). In general, the hatching rates of the resting eggs preserved with CPA were higher than those without CPA and the slow freezing method was better than the rapid freezing method. However, the optimum CPA concentration for the hatching rate of the resting eggs varied with the freezing method and kind of CPA, and the CPA also affected the viability of the resting eggs. Dried resting eggs had a high, rapid hatching rate over 80%. The moisture content of the resting eggs cryopreserved in liquid nitrogen affected the hatching rate. Drying at $30^{\circ}C$ for 1 hour resulted in a high hatching rate of the resting eggs. In conclusion, drying at $30^{\circ}C$ for 1 hour and preservation in liquid nitrogen with the slow freezing method, without CPA, is recommended for a high hatching rate (ca. 95%) of rotifer resting eggs.

Effects of pH Change by CO2 Induction and Salinity on the Hatching Rate of Artemia franciscana

  • Salma, Umme;Uddowla, Md. Hasan;Lee, Gi-Hun;Yeo, Young-Min;Kim, Hyun-Woo
    • Fisheries and aquatic sciences
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    • v.15 no.2
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    • pp.177-181
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    • 2012
  • To understand the effects of lower pH levels due to elevated $CO_2$ and salinity, we designed and constructed a pH-control system that included automatic $CO_2$ infusion and measured the hatching rate of a crustacean model species, Artemia franciscana. The pH-control system was cost-effective and capable of performing animal tests in which pH fluctuated around $8.0{\pm}0.1$, with the temperature around $27{\pm}0.5^{\circ}C$. Hatching rate was observed under four different pH levels (7.0, 7.3, 7.6, and untreated control) combined with three salinity ranges (15, 25, and 35 ppt). The results demonstrated that lower pH levels led to decreased hatching rates regardless of salinity, and the minimum hatching rate was detected at pH 7.0 compared to the control (pH $8.0{\pm}0.1$), supporting the idea that OA has adverse effects on hatching rates and increases the risk of juveniles being introduced in the ecosystem. In contrast, salinity changes exhibited no synergistic effects with pH and had independent effects.