• Title, Summary, Keyword: Hair regrowth

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Effect of Seaweed Extract on Hair Growth Promotion in Experimental Study of C57BL/6 Mice (해조류 추출물의 발모효과에 관한 C57BL/6의 쥐 실험연구)

  • Ha, Won Ho;Park, Dae Hwan
    • Archives of Craniofacial Surgery
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    • v.14 no.1
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    • pp.1-10
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    • 2013
  • Background: Recently, substances from seaweeds have been widely used in hair growth solutions, and have been proven to be effective. Seaweeds have been documented to possess hair growth activity; however, no report on the effect of seaweed on hair regeneration has been issued to date. In this study, we investigated which exact substance of hair tonic made by JW-bio and our institute shows effects on hair growth by studying the mechanisms of candidate substances. Methods: The study was conducted to investigate the hair restoring effect of domestic natural substances; we categorized the candidate substances as seaweed, cereal, and herbal medicine. Five experimental groups were included in the study as follows: a saline group, a 50% ethanol group, seaweed group, a cereal group, and a herbal medicine group. Results: Three extracts (seaweed, cereal, and herbal medicine) were administered to C57BL/6 mice for two weeks after depilation. Depilated areas were found to be completely covered with fully grown hair, and the hair re-growth score was highest in the seaweed group. Using a hair analysis system, hair characteristics were measured in all groups on days 10 and 14 after depilation. The width and length of hair follicles were largest in the seaweed group. Groups treated with seaweed showed significantly increased gene expression of insulin-like growth factor-1. Groups treated with all the three extracts showed decreased expression of transforming growth factor-${\beta}1$. Conclusion: Findings from our study suggest that seaweeds possess hair-growth effects and may be useful for the treatment of alopecia in the future.

Comparison of Saccharina japonica-Undaria pinnatifida Mixture and Minoxidil on Hair Growth Promoting Effect in Mice

  • Park, Ki Soo;Park, Dae Hwan
    • Archives of Plastic Surgery
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    • v.43 no.6
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    • pp.498-505
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    • 2016
  • Background Algae have traditionally been used for promotion of hair growth. Use of hair regrowth drugs, such as minoxidil, is limited due to side effects. The aim of this study was to examine a mixture of Saccharina japonica and Undaria pinnatifida (L-U mixture) on hair growth and to compare the promoting effect of hair growth by a 3% minoxidil and a L-U mixture. Methods To evaluate the hair growth-promoting activity, saline, 50% ethanol, 3% minoxidil, and the L-U mixture were applied 2 times a day for a total of 14 days on the dorsal skin of C57BL/6 mice after depilation. Analysis was determined by using a high-resolution hair analysis system, real-time polymerase chain reaction, and H&E staining. Results On day 14, the hair growth effect of the L-U mixture was the same as that of the 3% minoxidil treatment. The L-U mixture significantly (P<0.05) stimulated hair growth-promoting genes, as vascular endothelial growth factor (VEGF) and insulin-like growth factor -1. Increase of VEGF was observed in the L-U mixture group compared with minoxidil and the negative control. In contrast, the L-U mixture suppressed the expression of transforming growth factor-${\beta}1$, which is the hair loss-related gene. In histological examination in the L-U mixture and minoxidil groups, the induction of an anagen stage of hair follicles was faster than that of control groups. Conclusions This study provides evidence that the L-U mixture can promote hair growth in mice, similar to the effect from minoxidil, and suggests that there is potential application for hair loss treatments.

Seasonal Flank Alopecia in a Dog (개에서 발생한 계절성 옆구리 탈모증 1례)

  • Park, Seong-Jun
    • Journal of Veterinary Clinics
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    • v.31 no.4
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    • pp.341-343
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    • 2014
  • A 6-year-old female, shih-tzu was presented in the February with bilateral, symmetrical hair loss and hyperpigmentation on caudal dorsolumbar region. Skin test and blood examination found no remarkable finding. Histopathological examination of biopsies from the alopecic lesions revealed follicular atrophy, infundibular hyperkeratosis and some follicles were twisted truncated and dilated. The diagnosis of recurrent flank alopecia was based on clinical signs and supportive histopathology. Treatment with oral melatonin was started at 3 mg twice daily for 80 days, and by this time complete hair regrowth was observed. The following year in the September, a small non-inflammatory alopecia developed in the same site. The dog was given oral melatonin at 3 mg twice daily for 4 weeks, hair had completely regrown.

Protective Effects of Zizyphus jujuba and Fermented Zizyphus jujuba from Free Radicals and Hair Loss (대추 및 발효대추의 라디칼 소거능 및 모발 성장 촉진 효과)

  • Jung, Ji Eun;Cho, Eun Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.8
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    • pp.1174-1180
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    • 2014
  • This study investigated the antioxidative and hair growth-promoting activities of Zizyphus jujuba (Zj) and fermented Zj with Aspergillus oryzae, Bacillus subtilis, Bifidobacterium breve, Lactobacillus acidophilus, and Saccharomyces cerevisiae (Sac. cerevisiae). Among Zj and fermented Zjs, Sac. cerevisiae-fermented Zj (Zj-Y) exerted stronger scavenging activity against 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals than others. In addition, total polyphenol content of Zj-Y was higher than that of non-fermented Zj and other fermented Zj. This result indicates that fermentation of Zj by Sac. cerevisiae elevated antioxidative activity. Furthermore, using an alopecia model in C57B/6N mice, the hair growth activities of Zj and Zj-Y were investigated. The test samples, EtOH, minoxidil (MXD), Zj, and Zj-Y, were topically treated with 0.2 mL/day for 4 weeks. The experiments involved macroscopic observation and measurement of hair length methods. The results show that regrowth speed of hair was in decreasing order of MXD> Zj-Y> Zj> EtOH. The topical application of MXD and Zj-Y in mice promoted hair regrowth and prevented hair loss compared to the control group. The present study indicates that Zj-Y is a promising treatment for alopecia.

A Study for Verification of Hair Growth Effect of Azelaic Acid and Vitamin B6 (아젤라인산 및 비타민 B6의 육모효과 검증에 관한 연구)

  • Yoon, Sean Hyuck;Park, Dae Hwan;Sin, Jeong Im
    • Archives of Plastic Surgery
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    • v.35 no.5
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    • pp.507-513
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    • 2008
  • Purpose: Interest in the augmentation of hair growth for functional and aesthetic purpose has increased dramatically in recent years. Many hair growth products have been released, but most of these have not been proven scientifically. This study aims to measure the hair growth effect of azelaic acid and vitamin $B_6$, which have been known as hair growth materials, in animal models. Methods: Six weeks old C57BL/6 mice were used in this study and hair of mice were removed by topical treatment. The mice were divided into five experimental groups according to the testing material such as saline (negative control), propylene glycol(vehicle control), azelaic acid, vitamin B6 and azelaic acid plus vitamin B6 in combination. Hair growth was documented photographically and histologically, and then analysed by the high quality hair analysis program system. The quantity of endocrine factors, IGF-I and TGF-${\beta}1$ in the skin of mice was measured by PCR analysis. Results: The topical treatment of azelaic acid and vitamin B6 in combination for 2 weeks to dorsal skin accelerated hair regrowth more than other groups. The azelaic acid and vitamin $B_6$-combined treatment also promoted hair follicle elongation and thickness compared to the others. Histologic studies showed increased number of basal cells in azelaic acid and vitamin $B_6$-combined treatment. Furthermore, the azelaic acid and vitamin $B_6$-combined group significantly increased the expression of IGF-I but decreased the expression of TGF-${\beta}1$ in the skin of mice compared to other groups. Conclusion: These results suggest that azelaic acid and vitamin $B_6$, when used together, have an additive effect and might be used as hair growth materials.

The Contributory Roles of Th17 Lymphocyte and Cytotoxic T Lymphocyte at the Hair Bulge Region as Well as the Hair Bulb Area in the Chronic Alopecia Areata Patients

  • Hong, Jin-Woo;Lee, Chae-Young;Ha, Seung-Min;Choi, Seung-Hwan;Kim, Tae-Hoon;Song, Ki-Hoon;Kim, Ki-Ho
    • Annals of dermatology
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    • v.29 no.2
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    • pp.156-166
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    • 2017
  • Background: Alopecia areata (AA) is a T cell-mediated autoimmune disease that targets hair follicles and interrupts hair regrowth. The microenvironment of the effector T cells and their related cytokines may affect immunopathogenesis around the hair bulb/bulge. Objective: To determine the contributory roles of the effector T cell subsets and related cytokines to the pathogenesis of AA. Methods: We investigated the correlation between histopathological grades and four clinical prognostic factors in 331 patients with AA, and analyzed the topography of T cell infiltrates and related cytokines around the hair bulb/bulge according to histopathological grades through immunohistochemical and double immunofluorescence studies on a subset of AA specimens. Results: First, the groups with more severe histopathological grades were associated with earlier onset, longer duration, more hair loss, as well as poorer therapeutic outcomes. Second, the pattern of CD4 and CD8 expression around the hair bulb/bulge varied by histopathological grade, with staining density decreasing in the following order: type 1>type 2>type 3. In addition, $interferon-{\gamma}$ and transforming growth $factor-{\beta}1$ expression appeared denser in the peribulbar area. Interestingly, the denser $CCR6^+$ cells (Th17 cells) showed more infiltration than $CCR5^+$ cells (Th1 cells) around the hair bulb/bulge as histopathological grade worsened. Conclusion: The insidious destruction of bulge stem cells and hair bulb matrix stem cells results in more severe hair loss in patients with chronic AA, which is mediated by Th17 lymphocyte and cytotoxic T lymphocyte infiltration. Furthermore, Th17 lymphocytes may play an even more important role than cytotoxic T cells in the development of AA.

Effects of Crataegi Fructus Hot-Water Extract on Hair Growth and Growth Factor Gene Expression of Human Dermal Papilla Cells (산사 열수추출물의 모발 성장과 모유두세포의 성장인자 유전자 발현에 대한 영향)

  • Kim, Hyeong-Key;Kim, Cheol-Hong;Yoon, Hyun-Min;Kang, Kyung-Hwa;Song, Choon-Ho
    • Korean Journal of Acupuncture
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    • v.34 no.3
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    • pp.146-155
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    • 2017
  • Objectives : This study was carried out to investigate the effects of Crataegi Fructus water extract(CFWE) on hair growth in an alopecia model of C57BL/6N mice and human dermal papilla cells(hDPCs). Methods : Six-week old mice were depilated and separated in 3 groups ; CON, MXD(2% Minoxidil), and CFWE. The treatments were applied twice a day for 18 days. The hair growth was determined photographically. The hair density, thickness and length were identified by Folliscope and the weights of body were measured. In dorsal skin tissue, the expression of hair growth-related protein was analyzed by Western blot. In hDPCs with/without $IFN-{\gamma}$, cell proliferation and the expression of hair growth-related genes were analyzed. Results : We observed that CFWE promoted hair growth compared to CON. CFWE improved the hair density, thickness and length compared to CON. CFWE increased the $Wnt/{\beta}$-catenin signaling in dorsal skin. In hDPCs, CFWE accelerated the cell proliferation and inhibited $IFN-{\gamma}$-induced hDPCs degeneration. CFWE increased the mRNA expression of ${\beta}$-catenin, Axin-2, BMP-4, FGF-7, FGF-10, and ALP compared to CON and $IFN-{\gamma}$ treated cells. Conclusions : These results suggest that CFWE has a hair regrowth activity via $Wnt/{\beta}$-catenin signaling and can be useful for the treatment of alopecia.

Hair growth promoting effects of emodin in telogenic C57BL/6 mice

  • Yon, Jung-Min;Park, Seul Gi;Lin, Chunmei;Gwon, Lee Wha;Lee, Jong-Geol;Baek, In-Jeoung;Lee, Beom Jun;Yun, Young Won;Nam, Sang-Yoon
    • Korean Journal of Veterinary Research
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    • v.56 no.2
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    • pp.97-101
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    • 2016
  • Emodin is an anthraquinone derivative from the roots of Rheum officinale Baill that possesses a variety of biological activities, including inhibition of $5{\alpha}$-reductase and prostaglandin D2. In this study, we investigated whether emodin promotes hair growth. After emodin was topically applied to the shaved dorsal skin of telogenic C57BL/6 N mice, the hair growth rate and morphological analysis were evaluated in dorsal skin for 15 days. After 13 days of treatment, minoxidil or emodin (0.01% or 0.1%)-treated groups showed remarkable regrowth of hairs relative to the vehicle control group. Scoring of the hair growth and rate of hair growth area for 15 days revealed that groups treated with minoxidil and 0.1% emodin were significantly higher than the vehicle control group. Histological examination revealed the emodin and minoxidil groups markedly recovered the number and morphology of hair follicles, including the subcutis depth, relative to the vehicle group. These results suggest that emodin has an excellent promoting effect in hair growth similar to that of minoxidil and might be useful for treatment of baldness or alopecia.

Synergistic Effects of the Combination of Oral Herbal Composition and Topical Hair Tonic on Hair Growth in C57BL6 Mice (경구용 생약 복합 조성물과 경피용 육모 조성물의 복합 사용에 따른 육모 상승 효과)

  • Jeon, Hee-Young;Kim, Seung-Hun;Shin, Hyun-Jeong;Seo, Dae-Bang;Lee, Sang-Jun
    • Korean Journal of Food Science and Technology
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    • v.44 no.2
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    • pp.257-262
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    • 2012
  • Currently, various treatments available for alleviating hair loss and combination treatments are commonly used, which are frequently questionable and in effective. We aimed to investigate the synergistic effect regarding the combination of oral herbal composition and topical hair tonic on anagen induction and hair restoration in a shaving model of C57BL/6 mice. Seven-week old mice were trimmed by electric clippers and treated with oral herbal composition and topical hair tonic either alone or in combination. The combination treatment showed the highest hair growth promoting effect. Moreover, it significantly improved total blood antioxidant capacity and reduced lipid peroxidation and triglyceride level, which was not observed in the topical hair tonic treated group. These results suggest that the combination of oral herbal composition and topical hair tonic has a synergistic hair growth promoting effect and such synergism may be the result of the differing hair regrowth mechanisms concerning treatments.