• Title, Summary, Keyword: G-banding

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G-and C-Banding Pattern Analyses of Korean Rodents: I. Chromosome Banding Patterns of Striped Field Mice (Apodemus agrarius coreae) and Black Rats (R. rattus rufescens)

  • Koh, Hung-Sun
    • The Korean Journal of Zoology
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    • v.25 no.2
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    • pp.81-92
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    • 1982
  • G- and C-banding pattern analyses of striped field mice (Apodemus agrarius coreae) using 17 specimens from four localities in Korea revealed that centromeric heterochromatin results in the variation of No. 1 chromosome pair (telocentri $c_telocentric), i.e., centromeric heterochromatin sometimes appeared to be recognized as short arm. G- and C-banding patterns of four black rats (R. rattus rufescens) from two localities in Korea showed that No. 1 chromosome polymorphism (telocentri $c_telocentric) is due to pericentric inversion. In addition, G- and C-banding patterns of black rats mentioned above are idiogrammed.ammed.

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A comparative Karyotype study on Korean Squirrels. II. Karyotype Analysis of Sciurus vulgaris coreae and Tamias sibiricus asiaticus by G-banding Method. (한국산 다람쥐 핵형의 비교연구 II. G-banding 방법에 의한 한국산 청서(Sciurus vulgaris coreae)와 다람쥐(Tamias sibiricus asiaticus)의 핵형분석)

  • 김종봉;이희영
    • The Korean Journal of Zoology
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    • v.33 no.3
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    • pp.255-259
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    • 1990
  • The Karyotypes of Korean Sciurus uulgaris coreae and Tamias sibiricus asiaticus were analyzed by the G-banding method. Chromosomes of two species could be identified by G-banding patterns. The banding patterns of chromosomes 9, 10, 12 and X of S. vulgaris coreae were identical to those of chromosomes 6, 9, 12 and X, respectively of T. sibiricus asiaticus. It was shown that chromosomes 4, 10, 7 and 17 of T. sibiricus asiaticus resulted from pericentric inversion of chromosomes 1, 7, 8 and 16 of S. vulgaris coreae. These results suggested that pericentric inversion was an important factor in the karyological differentiation of two species.

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Identification of Chromosomal Band Marker of Korean Native Chick by High-resolution Banding Technique (고분염분석법(High-resolution banding)에 의한 한국재래계의 염색체 분염 표지 설정)

  • 백규흠;손시환
    • Proceedings of the Korea Society of Poultry Science Conference
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    • pp.85-87
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    • 2000
  • To obtain the genetic information of Korean native livestock, the karyotyping of Korean native chick were performed by high-resolution banding technique. The chromosomes were prepared from lymphocyte culture and early embryos with 200 Korean native chick which have been raised at National Livestock Research Institute. There were no significant difference between Korean native chick and Leghorn in the number of chromosomes and chromosomal morphological pattern. Using high resolution banding technique, the yield of G-bands of prophase is much greater than that can be obtained by International System for Standardzed Avian Karyotypes(ISSAK, 1999). The G-band landmarks of Korean native chick were similar to those of ISSAK and Leghorn except some macrochromosomes. chromosome Z and 3 had C-band variants with heteromorphic patterns on distal and centromeric site. The proportion of constitutive heterochromatin, the heterochromatin ratio of Korean native chick was significantly more than that of Leghorn in all chromosomes.

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The G-, C-, and NOR-Banded Karyotypes of Korean Short-hair Cat(Felis catus) (G-, C-Banding 및 NORs 분포 양상에 따른 한국산 단모고양이(Felis catus)의 핵형분석)

  • Cho, Eun Jung;Kong, Il Keun;Sohn, Sea Hwan
    • Reproductive and Developmental Biology
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    • v.37 no.3
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    • pp.161-167
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    • 2013
  • The karyotype of Korean short-hair cat was presented using the G-, C- and NOR-banding techniques. For chromosomes preparation, the fetus skin fibroblast cells were cultured and metaphases were obtained. In results, the Korean short-hair cat had 38 chromosomes with XX or XY, which consisted of 5 pairs of metacentric chromosomes(Group A and C), 3 pairs of submetacentric chromosomes (Group B), 6 pairs of medium metacentric chromosomes except for 1 pair of medium submetacentric D2 chromosomes (Group D, E), 2 pairs of acrocentric chromosomes(Group F) and metacentric X and Y sex chromosomes. In G-banding analysis, the Korean short-hair cat exhibited a typical and identical G-banding pattern in each homologous chromosome. Total number of bands and landmarks on the G-banded chromosomes of Korean short-hair cat well correspond to those of international standardization of karyotype of domestic cat. The heterochromatins of Korean short-hair cat chromosomes distributed at terminal and/or centromere regions on almost chromosomes by C-banding analysis. In addition, the C-banding pattern showed greatly heteromorphic in some chromosomes. Using the AgNOR-staining, we found the nucleolar organizer regions(NORs) of Korean short-hair cat located at chromosomes 1p12 site in E group. The quantity and number of NORs were constant among cells.

Studies on the Chromosomal Banding Analysis of Korean Native Fowl (한국재래계의 염색체 분양분석에 관한 연구)

  • 오희정;오봉국
    • Korean Journal of Poultry Science
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    • v.16 no.4
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    • pp.201-207
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    • 1989
  • This study was carried out to identify the chromosome morphological structure and G-, C-banding pattern of Korean native fowl. The samples used in this study were early chick embryos, and the method of chromosomal analysis quoted from the protocal of Ohio univ. with more or less modified. The results were summerized as follow as; 1. In each of macrochromosomal morphology, the arm-ratio, centromeric index, and relative length of Korean native fowl were more or less different from improved breeds, but the designations were the same. 2. The graphical pecks, by densitometric recordings, in each macrochromosome number of 1, 2, 3, 4, Z, and 5, numbered 21, 14, 12, 8, 11, and 4 in G-banded, and 16, 13, 9, 9, 9, and 4 in C-banded, respectively. Those pecks could be explained as a consequence of chromosome condensation during mitosis and of genetic material differences.

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Methodology of Chromosome Preparation and Banding Analysis in Gallus domesticus (닭 염색체의 분리 분석 방법에 관한 연구)

  • 손시환;오봉국
    • Korean Journal of Poultry Science
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    • v.14 no.2
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    • pp.89-96
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    • 1987
  • The purpose of this paper to present morphological normal chick chromosomes and develope avian cytogenetic techniques including chromosome preparation and banding technique. The early chick embryos provide a consistent source of material with hish mitotic cells. Although chick embryo tissue gives excellent preparations, the 4-5 days embryo is somewhat incovenient materials, Most imp of ant for avian Chromosome analysis are the technical protocols to achieve adequate preservation, spreading, and staining of the full chromosome complement. To precise chromosome analysis, pro-metaphase states are required. Best results of banding will be obtained from air dried slides prepared from early chick embryos that have been aged at least 1 week. Good G-banding differentiation is achieved by adequate trypsin digestion fellowed by staining in Goe,sa dye. The results of C-banding is influenced by many factors including the conditions of Ba(OH)$_2$, HCl treatment, and state of rinsing. In addition to precisely interprets banding patterns, the densitometric analysis is recommended.

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Chromosome Analysis by GTG, High-Resolution, and NOR-banding Techniques in the Dog (Cams familaris) (GTG, High-Resolution, Nor-banding에 의한 개의 염색체 분석)

  • 김종봉;윤인숙
    • Journal of Life Science
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    • v.12 no.5
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    • pp.605-609
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    • 2002
  • None of the numerous published canine idiograms and karyotypes has yet been generally accepted as a standard one because the dog has 76 acrocentric autosomes of similar size and shape. To establish canine banded karyotype from the 22nd chromosome to the 37th chromosome, we analyzed canine chromosomes by GTG, high resolution, and NOR-banding techniques. The GTG and high resolution banding patterns of canine chromosomes corresponded to other reports described previously except for a few chromosomes. While other researchers observed 12 bands, we observed 7 bands in the banding patterns of chromosome 24, 34 and 37. On the other hand, the banding patterns by NOR-banding technique showed that three pairs of autosomes have nucleolus organizer regions at the terminal ends of their long arm, and the Y chromosome has it in its short arm terminal. However, the X chromosome has no nucleolus organizer like other mammals.

Chromosome analysis by GTG banding technique in the DongGyeongi dogs (GTG banding에 의한 경주지방의 무미 또는 단미 형태의 개(경주개 동경이)의 핵형분석)

  • Choi, Seog-Kyu;Sung, Ki-Chang;Lee, Eun-Woo;Park, Chang-Eun
    • Korean Journal of Veterinary Service
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    • v.33 no.2
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    • pp.207-211
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    • 2010
  • None of the numerous published canis idiogram and karyotypes has yet been generally accepted as a standard one because the dog has 76 acrocentric autosomes of similar size and shape. The karyotypes of DongGyeongi dog were analysed by conventional trypsin/Giemsa staining (GTG-banding techniques), and were compared with one another. There were no variations in karyotypes which were analysed by conventional GTG-banding techniques, but differences were observed in G-banding patterns with sapsaree (or canis familiaris strains). It is not clear that these disagreements in G-banding patterns between strains of dog were caused by chromosome polymorphism or a difference in interpretation.

Karyotype of Jeju Horse; G-, C- and NOR-banding (제주재래마의 핵형분석; G-, C- 및 NOR-banding)

  • Park, Jin-Sik;Cho, Byung-Wook;Sohn, Sea-Hwan
    • Journal of Animal Science and Technology
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    • v.51 no.5
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    • pp.361-368
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    • 2009
  • This study was carried out to establish the standard karyotype of Jeju horse by G-, C- and AgNOR-banding patterns. Blood samples were collected from 37 Jeju horses and 24 Thoroughbred that had been raised at the National Institute of Subtropical Agriculture in Jeju. The lymphocytes were cultured in vitro and then chromosomes prepared. The diploid chromosome number of Jeju horse is 64, which consists of 31 pairs of autosomes and X, Y sex chromosomes. The Jeju horse has 13 pairs of metacentric/submetacentric and 18 pairs of acrocentric autosomes. The X chromosome is the fifth largest submetacentric, while the Y chromosome is one of the smallest acrocentric chromosomes. The G-banding pattern of Jeju horse chromosomes showed a light band at centromeres in all autosomes, and also exhibited a typical and identical banding pattern in each homologous chromosome. Overall chromosomal morphology and positions of typical landmarks of the Jeju horse were virtually identical to those of International Committee for the Standardization of the Domestic Horse Karyotype. C-bands of Jeju horse chromosomes appeared on centromeres of almost all autosomes, but chromosome 8 showed a heterochromatin heteromorphism. The NORs in Jeju horse chromosomes showed polymorphic patterns within breed, individuals and cells. By the AgNOR staining, the NORs were located at the terminal of p-arm on chromosome 1 and near centromeres on the chromosome 26 and 31. The mean number of NORs per metaphase was 4.68 in Jeju horse.

Studies on the G-banding Patterns of Normal and of Delayed Spiralized Chromosomes by BUdR in Dwarf Hamsters (Dwarf Hamster의 正常染色體와 BUdR에 의해 凝縮遲延된 染色體의 G-banding Pattern에 대한 硏究)

  • Hahn, Sahsook
    • The Korean Journal of Zoology
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    • v.18 no.2
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    • pp.71-86
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    • 1975
  • The G-banding patterns of normal and of delayed spiralized chromosomes by BUdR were investigated in three established cell lines of dwarf hamsters. The results obtained were as follows: 1. The number of G-bands appeared in Chinese hamster T-233 cell line was 65. The centromeric dark band was found in No.1 chromosome and weakly stained bands were also observed in part of the centromeric regions of Nos. 2, 3, 8 and $X_2$ chromosomes. Two homologous X chromosomes were found in different banding patterns. Terminal dark bands were shown in No. 1 chromosome. No conspicuous bands appeared in No. 10 chromosome. 2. Eighty four bands appeared in Armenian hamster Y-1249 cell line. Centromeric dark bands were observed in Nos. 5 and 10 chromosomes and moderatly stained bands were also found in near the centromeric region of the long arms of Nos. 7 and 9 chromosomes. Two isomorphic X chromosomes were also distinguished by their banding patterns. 3. In Y-1313 Armenian hamster cell line, the bands were 69. No centromeric dark bands were observed in this cell line, but moderatly stained bands appeared in the centromeric area in the long arm of No. 9 chromosome. The banding patterns of these two cell lines of Armenian hamster were quite different and readily distinguished. Only No. 8 chromosome showed similar G-banding patterns. Although Nos. 5, 7 abd 8 chromosomes revealed the same number of bands in these two cell lines, the location and staining intensity were quite different. 4. Chromosomes of Nos. 1, 2, 6, $X_1$ and $X_2$ in T-233 cell line and of 1, 4, 7, 8, 9, $X_1$ and $X_2$ in both cell lines of Armenian hamster were found to be elongated due to the inhibition of mitotic spiralization by BUdR. G-banding patterns of these chromosomes were found to be identical to those of normal chromosomes in these cell lines.

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