• Title, Summary, Keyword: G-Rh2

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Studies on Local Irritation of DA-3030, a new granulocyte colony stimulating factor (새로운 과립구 콜로니 자극인자(rhG-CSF) DA-3030의 국소자극성에 관한 연구)

  • 김옥진;안병옥;이순복;김원배;양중익
    • Biomolecules & Therapeutics
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    • v.2 no.3
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    • pp.247-255
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    • 1994
  • As a series of safety studies of DA-3030, a new rhO-CSF, its local irritancy was examined in the rabbits after the following treatment; application into the conjunctival sac of the eye(single), subcutaneous injection(single), intramuscular injection(single), and intravenous injection(8-day repeated). In addition, paravenous irritation of DA-3030 was investigated in mice. The results obtained were as follows. 1. In the result of ocular irritation test, 0.03% solution of DA-3030 could be considered as a non-irritating material. 2. The local irritation of DA-3030 by an injection of 0.5mι of its solution subcutaneously or intramuscularly was negligible and not so much different from that of saline. 3. In the vascular irritancy test, macro- and microscopic observations revealed that the irritating activity of DA-3030 in blood vessels was not different from that of saline when they were injected once a day into vein retroauricularis of rabbits for 8 days.4. The paravenous administration of DA-3030 did not induce any abnormal changes at injection sites except mild swelling in 1 mouse at 3 hours after injection which was thought to be due to slow absorption. The above-mentioned results suggest that DA-3030 has no irritating activity when injected through intravenous or subcutaneous route for clinical practice as 0.03% solution.

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Effects of Storage Conditions on Weight Loss and Free Sugar Composition of Fresh Ginseng (보관조건에 따른 수삼의 중량과 유리당 조성 변화)

  • Chang, Jin-Kyu;Kim, Cheon-Sug;Nho, Kil-Bong;Cho, Byung-Goo
    • Journal of Ginseng Research
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    • v.29 no.2
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    • pp.113-118
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    • 2005
  • This study was to examine the effect of the opening ratio (area of spile hole/total surface area) of plastic container and storage temperature on physicochemical changes of fresh ginseng. At room temperature ($19\~23^{\circ}C$, RH $40\~61\%$), fungi and spoilage were observed 4 and 10 days, respectively after storing the fresh ginseng in a closed container. In storage container with $1\%$ opening ratio, fungi appeared 8 days after storage. In the container with $5\%$ and $10\%$ opening ratio, fresh ginseng showed excessive loss of moisture on the surface only after 4 days. The weight loss was most remarkable in the container with 5 and $10\%$ opening ratio. Content of maltose and sucrose decreased gradually in accordance with storage time but the extent of decreasing ratio was less significant in the container with lower opening ratio. At low temperature $(4\~9^{\circ}C,\;RH\;72\~92\%)$, no fungi but $10\%$ loss of weight was observed in the container with $5\%$ and $10\%$ opening ratio after 12 days storage. But in the tightly closed container and $1\%$ opening ratio for spiting, fresh ginseng showed good appearance even after 30 days of storage. Free sugars, especially maltose revealed gradual decrease but sucrose gradually increase following decrease at the beginning. This result suggests that storage condition of low temperature and opening ratio of plastic box less than $1\%$ can extend storage span of fresh ginseng significantly.

Redox reaction of Fe-based oxide mediums for hydrogen storage and release: cooperative effects of Rh, Ce and Zr additives (수소 저장 및 방출을 위한 Fe 계 산화물 매체의 환원-산화 반응: Rh, Ce 및 Zr 첨가제의 협동 효과)

  • Lee, Dong-Hee;Park, Chu-Sik;Kim, Young-Ho
    • Transactions of the Korean hydrogen and new energy society
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    • v.19 no.3
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    • pp.189-198
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    • 2008
  • Cooperative effects of Rh, Ce and Zr added to Fe-based oxide mediums were investigated using temperature programmed redox reaction (TPR/TPO) and isothermal redox reaction in the view point of hydrogen storage and release. As the results of TPR/TPO, Rh was a sale additive to remarkably promote the redox reaction on the medium as evidenced by the lower highest peak temperature, even though its addition was to accelerate deactivation of the mediums due to sintering. On the other hand, Ce and Zr additives played an important role to suppress deactivation of the medium in repeated redox cycles. The medium co-added by Rh, Ce and Zr (FRCZ) exhibited synergistic performance in the repeated isothermal redox reaction, and the amount of hydrogen produced in the water splitting step at 623 K was highly maintained at ca. $17\;mmol{\cdot}g^{-1}-Fe$ during three repeated redox cycles.

Effects of Recombinant truman Granulocyte Colony-Stimulating Factor(rhG-CSF) on Cyclophosphamide-induced Neutropenic Mice (호중구 감소증을 유도한 마우스에서의 유전자 재조합 인과립구 콜로니자극인자의 효과)

  • 조명행;유아선;방명주;곽형일;성하정;안길환
    • Biomolecules & Therapeutics
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    • v.6 no.2
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    • pp.145-150
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    • 1998
  • Administration of 3 type KGCs [recombinant human granulocyte colony-stimulating factor (rhG-CSF)] to mice with cyclophosphamide (CPA)-induced neutropenia for 4 consecutive days from the day after the CPA dosing (100 mg/kg) resulted in a dose dependent increase in the peripheral blood neutrophil count 6 hours after the final KGC injection. Within the KGC dose range of 0.1 to 40$\mu$g Per mouse Per day, there was a sigmoidal relationship between the logarithm of the dose and the peripheral blood neutrophil count (relative value for neutrophil count of the basal dose) in the treated mice. The sigmoidal relationship of test KGC preparations shows that there is a saturation point in terms of efficacy. Compared with e(fact of KGC-Orange, Green, and Blue, KGC-Orange recovers neutrophils more effectively than the others do.

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Antifungal Activity and Plant Growth Promotion by Rhizobacteria Inhibiting Growth of Plant Pathogenic Fungi (식물병원성 진균을 억제하는 근권세균의 항진균능과 식물생장촉진능)

  • Jung, Taeck-Kyung;Kim, Ji-Hyun;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.48 no.1
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    • pp.16-21
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    • 2012
  • Since many pesticides cause various health and environmental problems, alternative measures to replace them are needed, and the bacteria producing the antifungal substances can be one of them. In this study, several rhizobacteria were isolated and their antifungal activities against some important plant pathogenic fungi were examined. Pseudomonas otitidis TK1 and Paenibacillus peoriae RhAn32 inhibited the growth of Fusarium oxysporum f. sp. niveum and F. oxysporum f. sp. lycopersici by 49.8% and 45.6%, and 45.1% and 48.3%, respectively compared to those of the control. P. peoriae RhAn32 also decreased the growth of F. oxysporum f. sp. raphani by 37.5%. This growth inhibition might be due to the production of antifungal substances, such as siderophore, hydrogen cyanide and chitinase, which were produced by these rhizobacteria. P. otitidis TK1 also produced plant growth hormones indole acetic acid and indole butyric acid at $293.41{\mu}g/mg$ protein and $418.53{\mu}g/mg$ protein, respectively. When P. otitidis TK1 and B. cereus TK2 were inoculated together with F. oxysporum f. sp. lycopersici to the 4 weeks grown tomato seedlings and incubated additional 8 weeks, the stem lengths of tomato increased up to 45.7% and 55.3% and root lengths were raised to 64.9% and 60.8%, respectively than those of the control group. The wet weights increased by 118% and 182%, respectively compared to the control group.

Statistical Selection of Amino Acids Fortifying a Minimal Defined Medium for a High-level Production of the Kringle Fragments of Human Apolipoprotein(a)

  • Lim, Hyung-Kwon;Kim, Sung-Geun;Jung, Kyung-Hwan;Seo, Jin-Ho
    • Journal of Microbiology and Biotechnology
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    • v.14 no.1
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    • pp.90-96
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    • 2004
  • A synthetic defined medium, fortified with amino acids, was developed for the stable production of the kringle fragments of human apolipoprotein(a) (apo(a)), rhLK68. Using a complex rich medium containing yeast extract and a high-cell-density fed-batch culture, the expression level of rhLK68 reached 17% of the total cellular protein, which corresponded to $5\;g\;l^{-1}$ of the culture. To replace the complex media with chemically defined media, several amino acids that positively affect cell growth and gene expression were chosen by a statistical method. The various combinations of the selected amino acids were tested for its fortifying effect on a minimal defined medium. When glutamine only was added, the overall expression level of rhLK68 reached 93% of the complex rich medium increasing the specific expression level by 22.4% and decreasing the cell growth by 24%. Moreover, the addition of glutamine resulted in a 2-fold increase in the concentration of rhLK68 in the culture broth, compared with the minimal defined medium. The synthetic defined media developed in this study could be generally applied to high-cell-density cultures of the recombinant Escherichia coli BL21(DE3), especially for the production of therapeutic proteins that require a strict quality control of the culture media and fermentation processes.

A Fermented Ginseng Extract, BST204, Inhibits Proliferation and Motility of Human Colon Cancer Cells

  • Park, Jong-Woo;Lee, Jae-Cheol;Ann, So-Ra;Seo, Dong-Wan;Choi, Wahn-Soo;Yoo, Young-Hyo;Park, Sun-Kyu;Choi, Jung-Young;Um, Sung-Hee;Ahn, Seong-Hoon;Han, Jeung-Whan
    • Biomolecules & Therapeutics
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    • v.19 no.2
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    • pp.211-217
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    • 2011
  • Panax ginseng CA Meyer, a herb from the Araliaceae, has traditionally been used as a medicinal plant in Asian countries. Ginseng extract fermented by ginsenoside-${\beta}$-glucosidase treatment is enriched in ginsenosides such as Rh2 and Rg3. Here we show that a fermented ginseng extract, BST204, has anti-proliferative and anti-invasive effects on HT-29 human colon cancer cells. Treatment of HT-29 cells with BST204 induced cell cycle arrest at $G_1$ phase without progression to apoptosis. This cell cycle arrest was accompanied by up-regulation of tumor suppressor proteins, p53 and p21$^{WAF1/Cip1}$, down-regulation of the cyclin-dependent kinase/cyclins, Cdk2, cyclin E, and cyclin D1 involved in $G_1$ or $G_1/S$ transition, and decrease in the phosphorylated form of retinoblastoma protein. In addition, BST204 suppressed the migration of HT-29 cells induced by 12-O-tetradecanoylphorbol-13-acetate, which correlated with the inhibition of metalloproteinase-9 activity and extracellular signal-regulated kinase activity. The effects of BST204 on the proliferation and the invasiveness of HT-29 cells were similar to those of Rh2. Taken together, the results suggest that fermentation of ginseng extract with ginsenoside-${\beta}$-glucosidase enhanced the anti-proliferative and the anti-invasive activity against human colon cancer cells and these anti-tumor effects of BST204 might be mediated in part by enriched Rh2.

Relationship between Weather Factors and Chemical Components of Flue-cured Tobacco (기상요인과 황색종 잎담배의 화학성분과의 관계)

  • Kim Sang-Beom;Cho Soo-Heon;Chung Youl-Young;Jeong Kee-Taeg
    • Journal of the Korean Society of Tobacco Science
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    • v.26 no.2
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    • pp.93-101
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    • 2004
  • This study was conducted to investigate the relationship between weather factors during the growing season and chemical components of flue-cured tobacco. Chemical components used in this study was from 'Farm Leaf Tobacco Test' conducted at KT&G Central Research Institute from 1986 through 2003. Data of weather factors during growing season(April to July) were collected in 10 districts measured at Korea Meteorological Adminstration(KMA). Nicotine and total sugar contents, and total sugar to nicotine(TS/Nic.) ratio were increased, whereas total nitrogen to nicotine(TN/Nic.) ratio and chloride content were decreased from 1986 through 2003. Year to year variation of rainfall was the largest, followed by that of sunshine hour. Month to month variation of rainfall also was the largest, followed by that of mean daily air temperature(MDAT). Rainfall was correlated positively with relative humidity(RH), but negatively with sunshine hour. Nicotine content was correlated positively with MDAT(in July, June$\~$July, May$\~$July and average), but negatively with rainfall(in May$\~$July) and with RH(in June, July, May $\~$June, June $\~$July, April$\~$June, May $\~$July and average). Total sugar content was correlated positively with MDAT(in May), but negatively with sunshine hour(average) and RH(in June, July, June$\~$July, April$\~$June, May$\~$July and average). The positive correlation was found between total nitrogen content and sunshine hour(in April, May, April$\~$May, May$\~$July, April$\~$June and average). The negative correlation was found between TS/Nic. ratio and sunshine hour(in May$\~$July and average). TN/Nic. ratio was correlated positively with sunshine hour(in May and April$\~$May) and with RH(in July and June$\~$July), but negatively with MDAT(in July, June$\~$July, May$\~$July and average). Ether extraction content was correlated positively with MDAT(in July, June$\~$July, May$\~$July and average) and with sunshine hour(in July and June$\~$July), but negatively with rainfall(in April, July, May­July and average). Chloride content was correlated positively with sunshine hour(in April, July, April$\~$May, June$\~$July, April$\~$June, May$\~$July and average), but negatively with rainfall(in April, April$\~$June and average).

Blood Analysis for Indirect Doping Control of Erythropoietin in Sports (운동선수들의 혈액분석을 통한 Etrythropoietin 간접도핑검사)

  • 이정란;김소영;홍지연;김명수;최명자
    • YAKHAK HOEJI
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    • v.47 no.6
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    • pp.422-431
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    • 2003
  • The use of recombinant human erythropoietin (rhEPO), a stimulator of erythropoiesis, banned in sports because of the medical risk associated with thrombosis. Due to analytical difficulties to differentiate between natural human EPO (hEPO) and rhEPO, blood parameters of erythropoiesis such as contents of hemoglobin (cut-off value <17.5 g/d l for man, and < 16.0 g/dl for women), hematocrit and reticulocytes (cut-off value <2.0%) were measured to focus the misuse of rhEPO. We conducted anti-doping test for 122 blood samples of the World Cup athletes. The mean values of key parameters are as follows; 14.5$\pm$1.0 g/dl for hemoglobin, 41.7$\pm$2.8% for hematocrit, and 1.3$\pm$0.4% for reticulocyte. Blood sample was found to be stable up to 8 hours for the reticulocyte measurement. In addition, the soluble transferrin receptor and ferritin levels were measured by immunoassay methods using plasma samples (n=28) in which the mean value was 0.8$\pm$0.5 $\mu\textrm{g}$/$m\ell$ and 54.6$\pm$33.7 ng/$m\ell$, respectively. The results indicate that all samples tested were negative for the blood parameters of indirect anti-doping test for hEPO misuse. The statistical evaluation suggest that several other parameters such as red blood cell, mean corpuscular hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin and white blood cell could be considered as factors influencing hEPO function in addition to five parameters mentioned.

Effects of Various Synchronization Methods on Postpartum Reproduction in Hanwoo (다양한 발정제어 방법이 한우의 분만성적과 분만후 차기번식에 미치는 영향)

  • Lee M. S.;Park J. J.;Jung Y. H.;Park S. B.;Suh G. H.;Kang M. J.;Moon S. J.;Kim C. K.
    • Journal of Embryo Transfer
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    • v.20 no.1
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    • pp.49-53
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    • 2005
  • This study was conducted to investigate the effects of repetition usage of various estrus synchronization and seasonal breeding in Hanwoo. Body weight(kg) at birth and weaning in calves produced by seasonal breeding are 24.0kg, 75.6kg in female, 24.9kg, 78.3kg in male, respectively. Changes of birth weight(kg) by parity in Hanwoo increased gradually. Raising rates of calves by raising experience of farmer higher than in more 10 years as $88.4\%$ and in more 6 years as $84.0\%$. Delivering rates by induced estrus methods in $PGF_{2}{\alpha}$, PRID, CIDR and $GnRH-PGF_{2}{\alpha}-GnRH$ were $87.0\%$, $87.3\%$, $91.6\%$ and $96.0\%$, respectively. Conception days of post-partum following to induced estrus methods in Control, $PGF_{2}{\alpha}$ PRID, CIDR and $GnRH-PGF_{2}{\alpha}-GnRH$ were 137.1, 147.6, 141.3, 116.6 and 118.0 days, respectively. The results show that repetition usage of various estrus synchronization were not effective on postpartum reproduction in Hanwoo.