• Title, Summary, Keyword: Formulation changes

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Aqueous Extract of Anticancer Drug CRUEL Herbomineral Formulation Capsules Exerts Anti-proliferative Effects in Renal Cell Carcinoma Cell Lines

  • Verma, Shiv Prakash;Sisoudiya, Saumya;Das, Parimal
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.18
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    • pp.8419-8423
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    • 2016
  • Purpose: Anti-cancer activity evaluation of aqueous extract of CRUEL (herbomineral formulation) capsules on renal cell carcinoma cell lines, and exploration of mechanisms of cell death. Materials and Methods: To detect the cytotoxic dose concentration in renal cell carcinoma (RCC) cells, MTT assays were performed and morphological changes after treatment were observed by inverted microscopy. Drug effects against RCC cell lines were assessed with reference to cell cycle distribution (flow cytometry), anti-metastatic potential (wound healing assay) and autophagy(RT-PCR). Results: CRUEL showed anti-proliferative effects against RCC tumor cell lines with an IC50 value of ${\approx}4mg/mL$ in vitro., while inducing cell cycle arrest at S-phase of cell cycle and inhibiting wound healing. LC3 was found to be up-regulated after drug treatment in RT-PCR resulting in an autophagy mode of cell death. Conclusions: This study provides the experimental validation for antitumor activity of CRUEL.

Evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers: implications on zootechnical parameters, serum biochemistry and liver histopathology

  • Selvam, Ramasamy;Saravanakumar, Marimuthu;Suresh, Subramaniyam;Chandrasekeran, CV;Prashanth, D'Souza
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.11
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    • pp.1795-1806
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    • 2018
  • Objective: The study was designed to establish choline deficiency model (CDM) in broilers for evaluating efficacy of polyherbal formulation (PHF) in comparison with synthetic choline chloride (SCC). Methods: A total of 2,550 one-day-old Cobb 430 broiler chicks were randomly assigned to different groups in three experiments. In experiment 1, G1 and G2 served as normal controls and were fed a basal diet with 100% soybean meal (SBM) as a major protein source supplemented with and without SCC, respectively. In G3, G4, G5, and G6 groups, SBM was replaced at 25%, 50%, 75%, and 100% by soy protein isolate (SPI) to induce a graded level of choline deficiency. In experiment 2, PHF (500 and 1,000 g/ton) in comparison with SCC (1,000 g/ton) were evaluated. In experiment 3, dose-response of PHF (200, 400, and 500 g/ton) with SCC (400 g/ton) was determined. Results: Replacement of SBM by SPI produced a linear decrease in body weight gain (BWG) with a poor feed conversion ratio (FCR). 25% SBM replacement by SPI yielded an optimum negative impact on BWG and FCR; hence, it is considered for further studies. In experiment 2, PHF (500 and 1,000 g/ton) and SCC (1,000 g/ton) showed a similar performance in BWG, FCR and relative liver weight. In experiment 3, PHF produced an optimum efficacy at 400 g/ton and was comparable to SCC in the restoration of serum aspartate aminotransferase activity, abdominal fat, breast muscle lipid content and liver histopathological abnormalities. Conclusion: Replacement of SBM by SPI caused choline deficiency characterised by worsening of BWG, FCR, elevation in liver enzymes and histopathological changes indicating fatty liver. CDM was found valid for evaluating SCC and PHF. It is concluded that PHF has the potential to mimic biological activities of SCC through the restoration of negative effects caused by CDM.

Effects of Retinyl Palmitate and Cholecalciferol Added Recombinant BST formulation Treatment on Milk Production and Health in Dairy Cows (Retinyl palmitate와 cholecalciferol이 첨가된 재조합 BST 제제 투여가 젖소의 산유량과 건강에 미치는 영향)

  • Kim, Nam-Joong;Chang, Byoung-Sun;Kim, Doo
    • Journal of Veterinary Clinics
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    • v.19 no.1
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    • pp.43-48
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    • 2002
  • This study was performed to investigate the effects of recombinant BST formulation treatment on the milk yield, milk components, mastitis, and general cow health condition when the formulations of retinyl palmitate, cholecalciferol and rBST were administered after the peak period of milk production. The milk yields of treatment groups (Group I, II, III and IV) were increased from 21.5% to 29.0% than that of control group. There was significant difference in milk production between treatment group II, IV and control group (P<0.05). However, there was no significant difference in milk production between treatment group I, III and control group (P<0.05). And the addition of retinyl palmitate and cholecalciferol into rBST formulation did not increase the milk yield. The milk of treatment groups with sustained-release rBST did not show significant difference in milk components (milk fat, protein, lactose, and solid not fat). However, there were minor changes, primarily in fat content of milk, during the first few weeks of rBST administration. There was no incidence of clinical mastitis between rBST treatment groups and control group. Addition of high and medium concentration of retinyl palmitate and cholecalciferol into sustained-release rBST formulation was efficient in reduction of somatic cell count in milk. There was great energy deficit in all treatment groups compared with control group during the early study period. Thus, the body condition score of all treatment groups showed lower value than control group. No evidences of metabolic health problems, such as ketosis, milk fever, and downer cow were observed. Incidence of general lameness did not appear on all treatment groups during 140 days of this study.

Solubilization of poorly water-soluble drugs using solid dispersions

  • Kim, Tae-Wan;Choi, Choon-Young;Cao, Qing-Ri;Lee, Beom-Jin
    • Proceedings of the PSK Conference
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    • pp.412.2-413
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    • 2002
  • Purpose. To prepare polymer based physical mixtures or solid dispersions containing solubilizing compositions using a spray-dryer. Methods. Lovastatin.simvastatin.aceclofenac and cisapride were selected as poorly water-soluble drugs. Dextrin. poly(vinylalcohol). poly(vinylpyrrolidone)and polyethylene glycol were chosen as solubilizing carriers for solid dispersions. The solid dispersions containing solubilizing compositions without drug were prepared without using organic solvents or tedious changes of formulation compositions. (omitted)

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DDM Rotordynamic Design Sensitivity Analysis of an APU Turbogenerator Having a Spline Shaft Connection

  • Lee, An-Sung;Ha, Jin-Woong
    • Journal of Mechanical Science and Technology
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    • v.17 no.1
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    • pp.57-63
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    • 2003
  • An eigenvalue design sensitivity formulation of a general nonsymmetric-matrix rotor-bearing system is devised. using the DDM (direct differential method). Then, investigations on the design sensitivities of critical speeds are carried out for an APU turbogenerator with a spline shaft connection. Results show that the dependence of the rate of change of the critical speed on the stiffness changes of bearing models of spline shaft connection points is negligible, and thereby their modeling uncertainty does not present any problem. And the passing critical speeds up to the 4th critical speed are not sensitive to the design stiffness coefficients of four main bearings. Further, the dependence of the rate of change of the critical speed on the shaft-element length changes shows quantitatively that the spline shaft has some limited influence on the 4th critical speed but no influence on the 1st to 3rd critical speeds. With no adverse effect from the spline shaft, the APU system achieves a critical speed separation margin of more than 40% at a rated speed of 60,000 rpm.

Finite Element Analysis of Damage Evolution in Drawing of Hardening Viscoplastic Metals (변형경화성을 갖는 점소성재의 인발공정에서 결함성장의 유한요소해석)

  • 함승연;이용신
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • pp.71-79
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    • 1994
  • Strip drawing of strain-hardening, viscoplastic materials with damage is analyzed by a rigid plastic finite element method. A process model is formulated using two state variables, one for strain hardening from slip dominated plastic distortion and the other for damage from growth of microvoids. Application of the model to steady state drawing is given via implementation in a consistent penalty finite element formulation. The predicted density changes as a result of void growth are compared to those from experiments reported in the literature. The effects of drawing conditions such as drawing speed and die angle on the mechanical property changes are studied.

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Trip-Chaining Behavior and Trip Distribution Model (연쇄통행행태분석과 통행분포모형)

  • 김형진
    • Proceedings of the KOR-KST Conference
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    • pp.58-82
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    • 1995
  • This study providesd an empirical analysis of trip-chaining behavior and its application to transportation planning. In the empirical analysis, changes in trip-chaining patterns since 1970 have been examined and details of current trip-chaining behavior as they describe shopping trip-chaining behavior has changed. Individual trip-chaining has become longer and complex. It appears that the average number of trips per chains has substantially increased over the past 20 years. An increased number of trips in chains means fewer home-based trips. Changes in trip-chaining behavior have several consequences. Important consequences are for transportation and land-use planning. Up to now trips have been treated as if they are independent clusters of home-to-destination-to-home; this approach has not usually taken into account the trip-chaining behavior of individuals. this calls for a different approach to at least the trip generation and trip distribution part of transportation planning. In this study, application of trip-chaining behavior to trip distribution model formulation is proposed and its calibration results are presented.

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The Study on the Aeroelastic Stability of Hingeless Helicopter Rotor in Hover Considering Parametric Angle Changes (파라메타 각 변화를 고려한 힌지없는 헬리콥터 로우터의 공력탄성학적 안정성)

  • 한창헌;김승조
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • pp.386-391
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    • 1998
  • The effect of the changes in parameter angles(precone, droop, sweep) on the lead-lag damping was focused on. Experiment was made with hingeless 4-blade rotors and NACA 0012 airfoil. For the measurement of the rotating natural frequencies and lead-lag damping, non-rotating swash plate was oscillated at the regressing lag mode frequency and the data were acquired after the excitation was cut off. Analysis was made using a finite element formulation based on Hamilton's principle. The main blade is assumed as elastic beams. Quasi-steady strip theory is used to obtain aerodynamic forces, and non-circulatory forces are also included.

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Acute Oral Toxicity of A Novel Combined Antibiotic(Cefatrizine / Clavulanic Acid) in Rats

  • Kwon, Jong-Won;Kang, Kyung-Koo;Hyun Cho;Baik, Nam-Gi;Ahn, Byoung-Ok;Kim, Gye-Won;Kim, Won-Bae
    • Toxicological Research
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    • v.14 no.4
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    • pp.501-505
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    • 1998
  • The acute toxicity study of combined antibiotic (Cefatrizine / Clavulanic Acid), a formulation consisting of cafatrizine and clavulanic acid in a ratio of 2 : 1, was evaluated in rats. The antibiotic was orally administered with single dose in dose levels up to 5 g/kg (0, 1.25, 2.5, 5 g/kg). Treatment-related effects were limited to soft stool excretion and caecal dilatation, but histologically no morphological changes could be detected in caecum. In hematology, serum-chemistry parameters and histopathology, no drug-related changes were found. The results of the present study indicate that cefatrizine / clavulanic acid has a low toxic potential and the oral $LD_{50}$values exceed 5 g / kg in rats

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Monitoring and Scheduling Methods for MIMO-FIFO Systems Utilizing Max-Plus Linear Representation

  • Goto, Hiroyuki;Masuda, Shiro
    • Industrial Engineering and Management Systems
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    • v.7 no.1
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    • pp.23-33
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    • 2008
  • This paper proposes an approach to monitoring and scheduling methods for repetitive MIMO-FIFO DESs. We use max-plus algebra for modeling and formulation, known as an effective approach for controller design for this type of system. Because a certain type of linear equations in max-plus algebra can represent the system's behavior, the principal concerns in past researches were how to solve the equations. However, the researches focused mainly on analyses of the relation between inputs and outputs of the system, which implies that the changes or the slacks of internal states were not clarified well. We first examine several properties of the corresponding state variables, which contribute to finding and tracing the float times in each process. Moreover, we provide a rescheduling method that can take into account delays or changes of the internal states. These methods would be useful in schedule control or progress management.