• Title, Summary, Keyword: Estradiol Benzoate

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Effects of Ovariectomy and Administration of Estradiol Benzoate on Body Growth and Development of Endocrine Glands in Female Rats (암 흰쥐에 있어서 난소척출 및 Estradiol Benzoate 투여가 체성장 및 내분비선 발육에 미치는 영향)

  • 정영호;정영채;김창근
    • Korean Journal of Animal Reproduction
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    • v.3 no.1
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    • pp.48-55
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    • 1979
  • This experiment was conducted to investigate the effects of ovariectomy and estradiol benzoate administration on body growth and development of endocrine glands in female rats. One hundred and twenty female rats, 30 days old weighing 72.0${\pm}$3.0g, were divided into control and ovariectomized groups and divided again into two groups, treated and untreated with estradiol benzoate, respectively. Each group was composed of 30 rats. Hormone was injected every two week interval. The body weight was measured weekly from the beginning of treatment to 63 days after treatment and 6 rats per group were sacrificed at 7, 21, 35, 49 and 63 days after treatment for weight change of endocrine gland. The results obtained were as follows: 1. the changes in the body weight during the experimental period were not signifilcantly different between two groups, although the body weight of ovariectomized groups were heavier than that of control groups and the body weight of treated group with estradiol benzoate was heavier than that of untreated group. 2. The body lengths in all groups were increased proportionally to body weight until 63 days after treatment and there were not significantly different among the experimental groups. 3. The weight of hypophysis treated with estradial benzoate in ovariectomized groups was continuously increased in the whole period and was highly significant at 49 and 63 days after treatment as compared with those of untreated in ovariectomized group. 4. Adrenal gland weights of the control and ovariectomized group treated with estradiol benzoate were distinctly light at 21 days after treatment, but there were not significantly different among all groups at 35 days. Adrenal gland weight of ovariectomized groups was smaller than control groups at 49 days and untreated group in ovariectomized groups was considerably smaller than the other 3 groups at 63 days after treatment. 5. In control rats, the ovary weight treated group with estradiol benzoate was larger than the untreated group at 35 days after treatment, but was smaller than the untreated group after 49 days. 6. The weight of reproductive tract in ovariectomized groups were markedly smaller than control groups till 35 days after treatment. The growth of reproductive tract untreated in ovariectomized groups was almost completely depressed.

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Studies on Artificial Control of Parturition in Korean Native Goats IV. The Effects of Dexamethasone and(or) Estradiol-Benezoate to Induction of Parturition (한국재래산양 분만의 인위적 조절에 관한 연구 IV. Dexamethasone 단독 및 Estradiol-Benzoate와의 병용투여에 의한 분만유기 효과)

  • 윤창현;성환후;오석두;장규태
    • Korean Journal of Animal Reproduction
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    • v.16 no.3
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    • pp.231-237
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    • 1992
  • The effects to the corticosteroid dexamethasone(DEX) and(or) estradiol-benzoate(E-B) treated to induction of parturition and birth weights of the young was conducted in 48 pregnant Korean native goats. The animals were divided into 4 goats per each treatment by the time(142, 145 and 148 day of pregnancy) and dosage(DEX 15, 20mg, DEX 8mg+E-B 10mg and DEX 13mg+E-B 7.5mg per goat). The results obtained were summarized as follows: 1 The time for induction of parturtion after DEX(15, 20mg) treatment on day 148 of pregnancy was significantly(P<0.01) shorter than 142 and 145 days. There was no significant difference each dosage of DEX on same day of pregnancy. 2. The addition of E-B to DEX treatment compared with DEX alone group at day 142 and 145 of pregnancy were significantly(P<0.01) shorter the time for induction of parturition. However, treatment with DEX along or with E-B on 148 day of pregnancy did not affect the time to induction(about 27 hrs) of parturition between each treatment. 3. The birth weight of kids after parturition was heaviest(P<0.01) on day 148 of pregnancy. However, development and vigor of kids were not significant different between DEX alone treatment and with E-B.

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Studies on Artificial Contorl of Parturition in Korean Native Goats III. The Effects of Prostaglandin $F_2\alpha$ and Estradiol-Benezoate (한국 재래산양 분만의 인위적 조절에 관한 연구 III. Prostaglandin $F_2\alpha$와 Estradiol-Benzoate 병용투여에 의한 분만수기 효과)

  • 윤창현;민관식;장규태;오석두
    • Korean Journal of Animal Reproduction
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    • v.16 no.2
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    • pp.109-116
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    • 1992
  • The present study was carried out to establish a practical regimen for artificial parturition induction using prostaglandin F2$\alpha$(PGF2$\alpha$) and estradiol-benezoate in Korean native goats. The effect of parturition induction and the time intervals to induced parturition after injection were investigated. The birth weight and body weight of kids at 15 days of age were measured. A total of 24 pluriparous goats were offered for this experiment. The animals were divided into 4 goats per treatment by the injection time(142, 145 or 148 day of pregnancy) and dosage(5.0$\times$10 or 7.5$\times$7.5mg). The results obtained were summarized as follows : A total of 24 pregnant goats were intramusculary treated with 5.0$\times$10 or 7.5$\times$7.5mg of PGF2$\alpha$ and estradiol-benzoate for parturition induction of Day 142, 145 or 148 of gestation. Parturition was induced in all of the goats(100%) treated. The kids produced from induced parturition were all healthy. The time intervals to induced parturition after PGF2$\alpha$ and estradiol-benezoate injection of 5.0$\times$10 or 7.5$\times$7.5mg to pregnant goats on Day 148(23.22$\pm$0.51~23.40$\pm$1.26hrs) were significantly(P<.01) shorter than those of the 142 days of the gestation(26.34$\pm$2.22~28.39$\pm$3.02hrs). No significant difference was found in the time intervals between the doses(5.0$\times$10 or 7.5$\times$7.5mg) treated for parturition induction. The birth weight of kids from induced parturition was no significant difference between on Day 148 and on Day 142 of gestation. However, the birth weight of kids from parturition induced on Day 148 was found significantly(P<.01) heavier than that of the 142 days of gestation. The body weight of kids at 15 days old was also significantly(P<.01) lighter in the parturition induced on day 142 than those on Day 142. The birth weight and body weight of kids at 15 days old were not affected by 5.0$\times$10 or 7.5$\times$7.5mg injection of PGF2$\alpha$ and estradiol-benzoate for inducing parturition. From the above results, it was concluded that the parturition induction by PGF2$\alpha$ and estradiol-benezoate injection of 5.0$\times$10 or 7.5$\times$7.5mg on Day 142 of gestation, which was correspondent to 8 days before expected spontaneous parturition, was available without any significant troubles.

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Analgesic Effects of Gyejibokryeong-hwan Aqueous Extracts on the Rat Model of Primary Dysmenorrhea (원발성 월경통에 대한 계지복령환(桂枝茯苓丸) 열수 추출물의 진통 효과)

  • Cho, Su-Yun;Kim, Dong-Chul
    • The Journal of Korean Obstetrics and Gynecology
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    • v.33 no.3
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    • pp.95-118
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    • 2020
  • Objectives: The objective of this in vivo study is to observe the analgesic effects or improvements of Gyejibokryeong-hwan aqueous extracts (GJBRHe) on the Primary dysmenorrhea (PD) in rats as compared to those of Indomethacin (IND). Methods: The rats were administered with estradiol benzoate for 10 days and oxytocin 1 hour after the last 10th administration of estradiol benzoate to make the primary dysmenorrhea rat model. Gyejibokryeong-hwan aqueous extracts 500, 250 and 125 mg/kg were orally administrated, for 10 days once a day. Then the changes on the body weights and gains during experimental periods, uterine weights and gross inspections, abdominal writhing response for analgesic activities, uterus lipid peroxidation (malondialdehyde (MDA) levels), antioxidant defense system - glutathione (GSH) contents, activities of superoxide dismutase (SOD) and catalase (CAT), Nuclear factor-κB (NF-κB) and Cyclooxygenase (COX)-2 mRNA expressions, were monitored with uterus histopathology and immunohistochemistry for tumor necrosis factor (TNF)-α and inducible nitric oxide synthase (iNOS). The results of Gyejibokryeong-hwan aqueous extracts were compared to those of Indomethacin adminstered rats. Results: As results of estradiol benzoate and oxytocin treatment, noticeable decreases of body weights and gains, uterus GSH contents, SOD and CAT activities, increases of abdominal writhing responses, uterus lipid peroxidation (MDA level), uterus weights, NF-κB and COX-2 mRNA expressions were observed with increases of TNF-α and iNOS immunolabeled cells, inflammatory cell infiltrations, congestion and enlargement of the uterus at gross and histopathological inspections. These means classic inflammatory and oxidative stress mediated primary dysmenorrhea are relatively well induced. However, these signs were favorably and dose-dependently inhibited by administration of three different dosages of Gyejibokryeong-hwan aqueous extracts, but lesser than those of Indomethacin. Conclusions: The results obtained in this study suggest that Gyejibokryeong-hwan aqueous extracts has favorable analgesic and refinement activities dose-dependently on the estradiol benzoate and oxytocin treatment-induced primary dysmenorrhea signs.

High Estradiol Differentially Affects the Expression of the Glucose Transporter Type 4 in Pelvic Floor Muscles of Rats

  • Carrasco-Ruiz, Maria de los Angeles;Hernandez-Aragon, Laura G.;Chavez-Rios, Jesus Ramses;Rodriguez-Antolin, Jorge;Pacheco, Pablo;Martinez-Gomez, Margarita;Cuevas-Romero, Estela;Castelan, Francisco
    • International Neurourology Journal
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    • v.22 no.3
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    • pp.161-168
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    • 2018
  • Purpose: To characterize the relationship between serum estradiol levels and the expression of glucose transporter type 4 (Glut4) in the pubococcygeus and iliococcygeus muscles in female rats. Methods: The muscles were excised from virgin rats during the metestrus and proestrus stages of the estrous cycle, and from sham and ovariectomized rats implanted with empty or estradiol benzoate-filled capsules. The expression of estrogen receptors (ERs) was inspected in the muscles at metestrus and proestrus. Relative Glut4 expression, glycogen content, and serum glucose levels were measured. Appropriate statistical tests were done to identify significant differences ($P{\leq}0.05$). Results: The pubococcygeus and iliococcygeus muscles expressed $ER{\alpha}$ and $ER{\beta}$. Glut4 expression and glycogen content in the pubococcygeus muscle were higher at proestrus than at metestrus. No significant changes were observed in the iliococcygeus muscle. In ovariectomized rats, the administration of estradiol benzoate increased Glut4 expression and glycogen content in the pubococcygeus muscle alone. Conclusions: High serum estradiol levels increased Glut4 expression and glycogen content in the pubococcygeus muscle, but not in the iliococcygeus muscle.

Expressional Changes of Connexin Isoform Genes in the Rat Caput Epididymis Exposed to Flutamide or Estradiol Benzoate at the Early Postnatal Age

  • Lee, Ki-Ho
    • Development and Reproduction
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    • v.21 no.3
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    • pp.317-325
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    • 2017
  • Direct communication between neighboring cells through connexin (Cx)-based gap junction is a crucial biological manner to regulate functions of a tissue consisting of multi-cell types. The present research evaluated expressional changes of Cx isoforms in the caput epididymis of adult rat exposed to estradiol benzoate (EB) or flutamide (Flu) at the early postnatal age. A single subcutaneous administration of EB at a low-dose [$0.015{\mu}g/kg$ body weight (BW)] or a high-dose ($1.5{\mu}g/kg\;BW$) or Flu at a low-dose ($500{\mu}g/kg\;BW$) or a high-dose (5 mg/kg BW) was performed to an animal at 1 week of age. Quantitative real-time PCR analysis was employed to determine expressional changes of Cx isoforms. The transcript levels of Cxs30.3 and 37 were decreased by a low-dose EB treatment, while decreases of Cxs31, 31.1, 32, 40, and 45 transcript levels were observed with a low-dose EB treatment. The treatment of a high-dose EB resulted in expressional reduction of Cxs30.3, 31, 31.1, 37, 40, 43, and 45. The Flu treatment at a low dose caused increases of Cxs26, 37, and 40 transcript levels but decreases of Cxs31.1, 43, and 45 transcript levels. Increases of Cxs30.3, 31, 37, and 40 mRNA amounts were induced by a high-dose Flu treatment. However, exposure to a high-dose Flu produced expressional decreases of Cxs31.1, 32, and 43 in the adult caput epididymis. These observations suggest that exposure to EB or Flu at the neonatal period could lead to aberrant expression of Cx isoforms in the adult caput epididymis.

Aberrant Expression of Cx Isoforms in the Adult Caput Epididymis exposed to Estradiol Benzoate or Flutamide at the Weaning

  • Lee, Ki-Ho
    • Development and Reproduction
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    • v.21 no.4
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    • pp.379-389
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    • 2017
  • Connexin (Cx) involves in the regulation of various physiological functions of tissue by forming a channel, a gap junction which allows direct cell-cell communication, between adjacent cells. The effect of a single subcutaneous treatment of estradiol benzoate (EB) or flutamide (Flu) at the weaning age on the expression of Cx isoforms in the adult caput epididymis was evaluated in this research. Using quantitative real-time PCR analysis, a low-dose of EB [$0.015{\mu}g/kg$ body weight (BW)] caused significant decreases of Cx30.3, Cx32, Cx40, Cx43, and Cx45 mRNA levels and no change of Cx26, Cx31, Cx31.1, Cx37 transcript levels. The treatment of a high-dose EB ($1.5{\mu}g/kg\;BW$) resulted in reduced expression of Cx30.3, Cx31, Cx43, and Cx45 but increased expression of Cx37 and Cx40. Expression of all Cx isoforms examined, except Cx31, was significantly increased by the treatment of a low-dose Flu ($500{\mu}g/kg\;BW$). However, the treatment of a high-dose Flu (5 mg/kg BW) led significant expressional suppression of Cx30.3, Cx31, Cx31.1, Cx32, Cx40, Cx43, and Cx45 but an increase of Cx37 transcript level. With the comparison of previous findings, the expression of Cx isoforms in the adult epididymis after the exposure to EB or Flu is likely differentially regulated in regional-specific and/or exposed postnatal age-specific manner.

Decreased Diethylnitrosamine-induced Liver Preneoplastic Lesions by Estradiol-3-benzoate Treatment

  • Kang, Jin-Seok;Park, Ki-Dae;Ahn, Byeong-Woo;Han, Beom-Seok
    • Toxicological Research
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    • v.27 no.4
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    • pp.247-251
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    • 2011
  • To clarify whether inhibitory effect of estrogen on liver tumor is associated with cell proliferation, we investigated its role in diethylnitrosamine (DEN)-induced rat preneoplastic lesions, with time sequenced manners. F344 male rats (n = 90) were divided into three groups at 5 weeks of age. The mini-osmotic pumps providing a continuous infusion of DEN was implanted into the abdominal cavity of each animal in group 1, 2 and 3 at 6 weeks of age. To see the effect of estrogen, pellet containing 1 or 10 ${\mu}g$ of estradiol-3-benzoate (EB) was implanted subcutaneously in the animals of groups 2 or 3, respectively, one week prior to DEN treatment. Ten animals of each group were euthanized at 10, 14 and 18 weeks after DEN treatment. Liver tissues at each time point were fixed in 10% phosphate-buffered formalin and were processed and embedded in paraffin and 5 ${\mu}g$ sections mounted on a silanized slide. Glutathione S-transferase placental form (GST-P) positive foci and 5-bromo-2-deoxyuridine (BrdU) labeling cells were detected at each time point. Area of GST-P positive foci in DEN+EB 1 or 10 ${\mu}g$ group was significantly decreased compared to DEN alone at 14 weeks (p < 0.01 or p < 0.05, respectively) an at 18 weeks (p < 0.05 or p < 0.01, respectively). BrdU index in DEN+EB 1 or 10 ${\mu}g$ groups was significantly decreased compared to DEN alone at 14 weeks and at 18 weeks (p < 0.01). Taken together, we conclude that EB treatment decrease the DEN-induced liver preneoplastic lesions and this may be associated with decrease of cellular proliferation.