• 제목, 요약, 키워드: Embryo Transfer

검색결과 1,946건 처리시간 0.028초

Embryo Collection, Transfer and Pregnancy of Riding Horses : First Successful Case in Korea

  • Park, Yong-Soo;Yang, Jae-Hyuk;Cho, Young-Jae;Oh, Dong-Yep;Cho, Gil-Jae
    • 한국수정란이식학회지
    • /
    • v.32 no.2
    • /
    • pp.59-64
    • /
    • 2017
  • Embryo transfer (ET) could be a relevant tool for genetic improvement programs in horses similar to those already underway in other species and produce multiple foals from the same mare in one breeding season. However, there have been no reports describing equine embryo transfer performed in Korea. In the present study, we performed an equine embryo collection and transfer procedure for the first time. We examined the embryo collection and pregnancy, size of embryo during the incubation period after collection, and progesterone (P4) and estradiol-$17{\beta}$ (E2) concentrations in mare's serum at embryo collection and transfer. A total of 16 donors responded to estrus synchronization; estrus was induced in 12 donors and 4 recipients, and artificial insemination was successful in 10 donors and six blastocysts were collected from donors. Of these blastocysts, we monitored the size of blastocysts for 3 day during incubation and transferred 2 blastocysts to a recipient, with 1 successful pregnancy and foal achieved. The dimensions of equine embryo at day 7 to day 9 were $409{\mu}m$, $814{\mu}m$ and $1,200{\mu}m$. The serum P4 and E2 concentrations were $7.91{\pm}0.37ng/{\mu}L$ and $45.45{\pm}12.65ng/{\mu}L$ in the donor mare, and 1$6.06{\pm}3.27ng/{\mu}L$ and $49.13{\pm}10.09ng/{\mu}L$ in the recipient mare.

소 수정란이식기술과 실용상의 문제점 (Bovine Embryo Transfer and Its Problems in Practical Use)

  • 김희석
    • 한국가축번식학회지
    • /
    • v.10 no.2
    • /
    • pp.145-160
    • /
    • 1986
  • Problems in the embryo transfer techniques and their practical a, pp.ication have been settle to some extent. However, with theorganic cooperation between universities and the related institutes for more active works on the training of embryo transfer technicians, quarantine procedures of embryos when they are imported and exported and the future researches related to the embryo transfer, the embryo transfer techniques in Korea will be more practically, a, pp.ied to animal industries. Universalization of embryo transfer techniques to the extent of artificial insemination will greatly contribute to the improvement of farm animals and multiplication of superior stocks and will save money by the import of relatively cheap embryos instead of expensive live animals.

  • PDF

돼지 수정란이식의 현황과 문제점 (Advances and Problems of Embryo Transfer in Swine)

  • 박창식
    • 한국수정란이식학회지
    • /
    • v.5 no.2
    • /
    • pp.11-22
    • /
    • 1990
  • Remarkable progress has recently been made in embryo transfer technology, resulting in the birth of IVF and nuclear transfer offsprings in swine. However, further progress of the technology to (I) make a safe, effective and economic estrual-cycle synchronization compound, (2) regulate each step of sperm capacitation (3) induce monospermic fertilization, (4) in vitro grow and mature oocytes, (5) fertilize the oocytes efficently, (6) culture the oocytes to the blastocyst stage in defined media, (7) produce multiply copies of embryos with superior genetic merit, (8) preselect the sex of these superior offsprings, and (9) preserve embryos by freezing and storage in liquid nitrogen is required before this promising technology is applied routinely to swine for practical use.

  • PDF

Successful Superovulation and Recovery of Embryos in Repeat Breeding Crossbred Cows

  • Zawar, Shyam;Mishra, U.K.;Gaswade, P.G.
    • 한국수정란이식학회지
    • /
    • v.17 no.3
    • /
    • pp.235-238
    • /
    • 2002
  • The present study was undertaken to find out possibilities for superovulation and recovery of freezable/transferable embryo from repeat breeding crossbred cows. For this study a total of 10 Holstein crossbred cows having history of failure of conception even after more than 4~5inseminations were taken and superovulated using Folltropin-V at 100 to 140% dose schedule. The results indicated that out of 10 donors, 8 responded to superovulatory treatment and yielding a total of 94 embryos, out of which 45 were of transferable/freezable quality. The mean ovulations and mean transferable embryos were 11.5$\pm$2.91 and 4.5$\pm$1.66, respectively. These observations suggests that the repeat breeding cows having conception failure after several artificial inseminations can be used as donors for production of embryos and calves through embryo transfer technology.