• Title, Summary, Keyword: EISA

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Monoclonal antibody-based enzyme immuno-slide assay (EISA) in the rapid diagnosis of Peste des petits ruminants of goats

  • Das, Kamol K.;Rahman, M.B.;Shil, N.K.;Rahman, Md Siddiqur;Jang, Hyung-Kwan;Song, Hee-Jong
    • Korean Journal of Veterinary Service
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    • v.33 no.1
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    • pp.1-6
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    • 2010
  • Monoclonal antibody (mAb)-based enzyme immune-slide assay (EISA) was used for the detection of Peste des petits ruminants (PPR) virus from field samples collected from a natural outbreak. The clinicopathological study was undertaken to diagnose the case primarily of PPR. Antigen was detected from discharges and faeces of infected goats and swabs of postmortem lesions prepared on glass slide or glass plate using acetone fixation. Nasal discharge collected at the early stage of disease course or lung is an appropriate ante- or postmortem sample for this technique, respectively. Convalescent polyclonal sera collected from recovered animals which were diagnosed as PPR by EISA showed high antibody titer against PPR by C-ELISA, demonstrating the satisfactory specificity of the test. Therefore, EISA is a sensitive and specific assay to confirm PPR infection both in field and laboratory conditions and especially suitable for developing country.

Derivation of Cubic and Hexagonal Mesoporous Silica Films by Spin-coating

  • Pan, Jia-Hong;Lee, Wan-In
    • Bulletin of the Korean Chemical Society
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    • v.26 no.3
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    • pp.418-422
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    • 2005
  • By introducing spin-coating method to the evaporation induced self-assembly (EISA) process, a simple and reproducible route in controlling the mesophase of silica thin films has been developed for the first time in this work. When a comparatively solvent-rich Si-sol (The atomic ratio of TEOS : F127 : HCl : $H_2O$ : EtOH = 1 : 0.006 : 0.2 : 9.2 : 30) was used as coating solution, the mesophase of resultant silica films was selectively controlled by adjusting the spin-on speed. The cubic mesophase has been obtained from the coating at a low rpm, such as 600 rpm, while the 2-D hexagonal mesophase is formed at a high rpm, such as 2,500 rpm. At a medium coating speed, a mixture of cubic and hexagonal mesophase has been found in the fabricated films. The present results confirm that the evaporation rate of volatile components at initial step is critical for the determination of mesopore structures during the EISA process.

Design of Traffic Data Acquisition System with Loop Defector and Piezo-Electric Sensor (루프검지기와 피에조 센서를 이용한 차량정보 수집 시스템 설계)

  • 한경호;양승훈
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.16 no.6
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    • pp.102-108
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    • 2002
  • This paper handles the design of a real time traffic data acquisition system using loop detector and piezo-electric sensor to acquire the vehicle information EISA compatible parallel I/O interface card is designed to sample 30 I/O channels at variable rates for raw traffic data acquisition. The control software is designed to generate the traffic data informations from the raw data. The traffic data information provides vehicle length, speed, number of axles, etc. Vehicle types are detected and categorized into eleven types from the vehicle length, axles positions and axle counts information. The traffic information is formed into packet and transferred to the remote hosts through serial communications for ITS applications.

QoS Implementation on a Clustering Web Server (클러스트링 웹서버에서의 QoS 구현에 관한 연구)

  • Park, Jong-Gyu;Lee, Do-Young;Chang, Whie;Kim, Hag-Bae
    • Proceedings of the KIEE Conference
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    • pp.2980-2982
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    • 2000
  • 본 연구에서는 리눅스 기반의 클러스터링 웹서버를 구성하고, 이 클러스터링 웹서버를 하나의 서버인 것처럼 관리하는 개발툴을 만들었다. 그리고 커널 패치를 통하여 로드밸런서가 다양한 시스템 정보를 밸런싱에 이용하도록 하였다. 각 리얼서버에는 응답 데이터의 양 혹은 각 패킷의 길이에 따라 전송의 순서를 결정하는 QoS를 구현하였다.

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A Distributed File System for Guaranteeing High Availability of a Clustering Web Server (웹 전용 리눅스 클러스터 서버의 고가용성을 위한 분산 파일 시스템에 대한 연구)

  • Park, Ji-Hyun;Ryu, Sang-Woo;Chang, Whie;Kim, Hag-Bae
    • Proceedings of the KIEE Conference
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    • pp.2965-2967
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    • 2000
  • 다양한 인터넷 응용 프로그램들이 웹 기반으로 통합되고 여러 방면에서 business-critical한 경우가 많아짐에 따라 웹서버의 고가용성과 안정성이 갈수록 강조되고 있고. 이를 보장하기 위한 리눅스 기반의 클러스터링 환경에서는 다양한 조건하에서도 데이터의 손실 없이 파일 입출력을 효과적으로 지원할 수 있는 분산 파일 시스템이 필수적이다. 본 논문에서는 리눅스 클러스터 환경에 적합한 분산 파일 시스템의 하나로서 카네기 멜론 대학에서 제안되어 개발 중에 있는 네트워크 분산 파일 시스템인 Coda 에 대하여. 가용성 및 효율성, 확장성 등에 대한 장단점을 간단히 소개하고, Coda을 적용한 고가용성 웹 서버의 구현 결과와 향후 개선 방향에 대해서 설명하도록 하겠다.

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Synthesis of Certain Mercapto and Aminopyrimidine Derivatives as Potential Antimicrobial Agents

  • El-Kerdawy, M.M.;Eisa, H.M.;El-Emam, A.A.;Massoud, M.A.;Nasr, M.N.
    • Archives of Pharmacal Research
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    • v.13 no.2
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    • pp.142-146
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    • 1990
  • Reaction of ethyl 4-chloro-2-phenylpyrimidine-4-carboxylate (4) with 5-chloro-2-methylthiophenol or 3-aryl-4-phenyl-1, 2, 4-triazole-5 thiol yielded the corresponding thioethers (5) and (8a, b), respectively. Careful alkaline hydrolysis of (5) yielded the corresponding carboxylic acid (6). Reaction of (4) with p-aminoacetophenone yielded compound (10) which was reacted with certain aromatic aldehyde to afford the$\alpha,\beta$-unsaturated ketones (11a-d). Condensation of (11a-d) with malononitrile or phenylhydrazine yielded the 2-amino-3-cyanopyridines (12a-f) or the 2-pyrazolines (13a, b) respectively. Seven representative compounds were tested for their in vitro antimicrobial activity against some pathogenic micro-organisms, some of them were proved to be active.

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Development of a Kernel Thread Web Accelerator (SCALA-AX) (커널 쓰레드 웹가속기(SCALA-AX) 개발)

  • Park, Jong-Gyu;Min, Byung-Jo;Lim, Han-Na;Park, Jang-Hoon;Chang, Whi;Kim, Hag-Bae
    • The KIPS Transactions:PartA
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    • v.9A no.3
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    • pp.327-332
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    • 2002
  • Conventional proxy web cache, which is generally used to caching server, is a content-copy based system. This method focuses on speeding up the phase delivery not improving the webserver performance. However, if immense clients attempt to connect the webserver simultaneously, the proxy web cache cannot achieve the desired result. In this paper, we propose the web accelerator called the SCALA-AX, whitch improves web server performance by accelerating the delivery contents. The SCALA-AX is built in the Linux-based kernel as a kernel modulo and works in combination with the conventional webserver program. The SCALA-AX speeds up the processing rate of the webserver, because it processes the requests using the kernel thread. The SCALA-AX also applies the well-developed cache algorithm to the processing, and thus it obtains the advantage of the caching server without installing additional hardware. A banchmarking test demonstrates that the SCALA-AX improves webserver performance by up to 500% for content delivery.

Soft-template Synthesis of Magnetically Separable Mesoporous Carbon (자성에 의해 분리 가능한 메조포러스 카본의 소프트 주형 합성)

  • Park, Sung Soo;Ha, Chang-Sik
    • Journal of Adhesion and Interface
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    • v.18 no.2
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    • pp.75-81
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    • 2017
  • In this study, we synthesized mesoporous carbon (Carbonized Ni-FDU-15) containing nanoporous structures and magnetic nanoparticles. Carbonized Ni-FDU-15 was synthesized via evaporation-induced self-assembly (EISA) and direct carbonization by using a triblock copolymer (F127) as a structure-directing agent, a resol precursor as a carbon-pore wall forming material, and nickel (II) nitrate as a metal ion source. The mesoporous carbon has a well-ordered two-dimensional hexagonal structure. Meanwhile, nickel (Ni) metal and nickel oxide (NiO) were produced in the magnetic nanoparticles in the pore wall. The size of the nanoparticles was about 37 nm. The surface area, pore size and pore volume of Carbonized Ni-FDU-15 were $558m^2g^{-1}$, $22.5{\AA}$ and $0.5cm^3g^{-1}$, respectively. Carbonized Ni-FDU-15 was found to move in the direction of magnetic force when magnetic force was externally applied. The magnetic nanoparticle-bearing mesoporous carbons are expected to have high applicability in a wide variety of applications such as adsorption/separation, magnetic storage media, ferrofluid, magnetic resonance imaging (MRI) and drug targeting, etc.