• Title, Summary, Keyword: EC-SOD

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The Detection of Superoxide Dismutase Activity and Isozyme Pattern of Panax ginseng C.A. Meyer Leaves (인삼엽에서 Superoxide Dismutase Activity 측정 및 Isozyme Pattern 검정)

  • Yang, Deok-Jo;Kim, Myeong-Sik;Lee, Seong-Jong
    • Journal of Ginseng Research
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    • v.11 no.1
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    • pp.24-31
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    • 1987
  • We studied a assay method on the measurement of superoxide dismutase (SOD Superoxide : superoxide oxidoreductase, EC. 1. 15. 1. 1) activity with photoreduced flavin and nitroblue tetrazolium (NBT) as superoxide (${O_2}^{-}$) source and detector, respectively. The $\Delta$E (1000 ng SOD$.$$min.)^{-1}$ of photoreduced flavin-NBT system was 0.08, whereas that of xanthine-xanthine-cytochrome system used broadly in experiments was 0.014. Therefore, the new method was regarded more simple and utilizable than xanthine-xanthine cytochrome system method. In the present paper, we also carried out to investigate the SOD activity and isozyme pattern for the parpose of study of leaf-burning disease in ginseng (Panax ginseng C.A. Meyer) leaves.

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Evaluation of Zinc and Copper Status in Korean College Women (일부 여대생의 구리와 아연 영양상태 평가)

  • 김정혜
    • Journal of Nutrition and Health
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    • v.32 no.3
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    • pp.277-286
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    • 1999
  • This study was conducted to assess dietary intake and nutritional status of zinc and copper in Korean college women. Dietary survey was conducted by 24-hour recall method and fasting serum samples were collected from 111 apparently healthy subjects. Intake levels of zinc and copper were calculated using newly developed database for Zn & Cu of Korea food. Serum levels of Zn, Cu and activities of ALP, EC-SOD were measured from fasting serum sample. Mean daily zinc and copper intakes were 6.72mg/day(56.0% RDA) and 1.11mg/day respectively. Mean values of serum ALP activity, zinc and copper concentration were 43.9U/L, 14.8umol/1, 15.5umol/1and these values were mostly within normal range. EC-SOD activitis of the subjects were low and had no correlation with intake or serum levels of Zn, Cu. In conclusion, these results show that zinc and copper intake of Koran college women are lower than those from other counties but higher than those of adults in rural area of Korea. Their serum levels of Zn, Cu, ALP are relatively normal. These results indicate that marginal deficiency of Zn and Cu may be quite prevalent in these subjects but serum indicators measured may not be sensitive enough to detect such marginal deficiency. Further study in needed to develop a biochemical index sensitive enough to evaluate Zn and Cu status.

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Antioxidant Compounds and Activities of Methanolic Extracts from Steam-Dried Allium hookeri Root (증포 삼채뿌리 메탄올추출물의 항산화 성분 및 항산화 활성)

  • Jun, Hyun-Il;Yang, Jae-Heon;Song, Geun-Seoup;Kim, Young-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.12
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    • pp.1725-1731
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    • 2016
  • This study was carried out to investigate the effects of steam-drying on antioxidant compounds and antioxidant activity of Alliun hookeri root (AHR). The yield of methanolic extracts, total phenolic content (TPC), browning intensity (280 nm and 420 nm), and organosulfur compound contents (alliin and cycloalliin) in raw and steam-dried AHRs were 10.71~37.40%, $15.53{\sim}36.36{\mu}g/mg$, 0.48~2.09, 0.01~0.25, $1.46{\sim}700.61{\mu}g/mg$, and $1,173.95{\sim}2,182.60{\mu}g/mg$, respectively. Yield, TPC, and browning intensity of AHRs increased by steam-drying, whereas organosulfur compound contents decreased. Of all methanolic extracts from raw and steam-dried AHRs, four-time steam-drying showed the lowest $EC_{50}$ values (0.43, 7.53, 0.34, and 0.48 mg/mL, respectively) for DPPH radical scavenging activity, ABTS radical scavenging activity, SOD-like activity, and reducing power, whereas four-time steam-drying resulted in the highest TPC ($36.36{\mu}g/mg$) and browning intensity (2.09 and 0.25 at 280 and 420 nm, respectively). The antioxidant activities of methanolic extracts from raw and steam-dried AHRs were closely correlated with their TPC, browning intensity, and organosulfur compound content, showing correlation determination coefficient ($R^2$) values higher than 0.82. As a result, four-time steam-drying of AHRs could be useful as potential antioxidant sources.

The Hepatoprotective Effects of Epimedii Herba through the Antioxidation (음양곽의 항산화작용에 의한 간 보호 효과)

  • Ha Bae Jin;Kim Hee Jin;Lee Sang Hun;Ha Jong-Myung;Lee Sang-Hyeon;Lee Jae-Hwa;Lee Dong-Geun;Park Eun Kyung;Nam Chun Suk
    • Journal of Life Science
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    • v.15 no.4
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    • pp.572-577
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    • 2005
  • In this study, the effect of Epimedii Herba (EH) on the antioxidative enzymatic activity was investigated. EH (100mg/kg) was intraperitoneally administered into rats for 2 weeks. On the last day, carbon tetrachloride $(50\%\;CCl_4,\;3.3ml/kg,\;i.p.)$ dissolved in olive oil was injected before 12 hours. EH-pread-ministered and $CCl_4-treated$ (EC) group showed higher inhibitory effect in aminotransferase (AST, ALT) activity compared to $CCl_4-treated (CT)$ group. Superoxide dismutase (SOD) and Catalase in EC group were increased compared to those of CT group. The activity of glutathione peroxidase (GPx) was significantly higher than those of CT group compared to EC group. These results suggest that EH has a hepatoprotective effect through scavenging the free radicals induced by $CCl_4$.

Antioxidative Effects of Solvent Fractions from Nandina domestica Fruits (남천(Nandina domestica) 열매 분획 추출물의 항산화 효과)

  • Seo, Soo-Jung;Shim, Kyu-Bong;Kim, Nam-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.10
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    • pp.1371-1377
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    • 2011
  • This study was carried out to compare the antioxidative effects of various Nandina domestica fruits extracts. Organic fractions, including n-hexane, chloroform, ethyl acetate, and butanol fractions, were obtained from the water and ethanol extracts of N. domestica fruits. The phenolic compound content of the EE fraction was 922.22 mg/g. The flavonoid compound content was highest in the EB fraction (282.49 mg/g). The electron-donating ability was highest (93%) in the WA and EH fractions at 0.1 mg/mL. The SOD-like activity was showed the highest in the EA fraction (56.36%), and EH and EC both showed higher than 50% activity. The nitrite-scavenging ability of the EC fraction at 1.0 mg/mL under pH 1.2 conditions was 82.03%. The xanthine oxidase inhibitory activities of all the fractions, except WE, were higher than 90% at 0.5 mg/mL. The effect of tyrosinase inhibition was highest in the WE fraction (46.75%). These results show that the N. domestica fruits fractions contained high levels of polyphenol and flavonoid compounds, along with excellent antioxidative effects. This suggests that N. domestica fruits can be used as a functional material.

Protective Role of Curcuma longa L. Extracts on Hydrogen Peroxide-Induced DNA Damage in Human Leukocytes (산화적 스트레스로 유도된 인체 백혈구 DNA 손상에 대한 울금 추출물의 보호효과)

  • Seo, Bo-Young;Park, Eunju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.5
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    • pp.545-551
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    • 2017
  • Curcuma longa L. (CL) is widely used as a spice and coloring agent in several foods, such as curry and mustard, as well as cosmetics and drugs. In this study, we investigated the protective effects of CL extracted with various solvents [methanol (MC), ethanol (EC), acetone (AC)] on $H_2O_2-induced$ DNA damage in human leukocytes along with total polyphenol contents (TPC) and antioxidant properties. The antioxidant effects of CL were determined by measuring 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (RSA) and superoxide dismutase (SOD)-like activity. The preventive effect of CL on oxidative stress-induced DNA damage and DNA repair capacities were assessed using comet assay. MC showed the highest TPC (11.17 g gallic acid equivalents/100 g) and antioxidant properties among the solvent extracts. The $SC_{50}$ for DPPH RSA was MC: 35.0 > AC: 45.8 > EC: $57.8{\mu}g/mL$ and SOD-like activity was MC: 46.6 > EC: 141.5 > AC: $296.4{\mu}g/mL$. In the comet assay, the $ED_{50}$ value of MC showed the highest inhibition ($86.7{\mu}g/mL$) of $H_2O_2-induced$ DNA damage, followed by AC ($110.0{\mu}g/mL$) > EC ($115.8{\mu}g/mL$). Analysis of the percentage of damaged cells showed that repair capacity significantly decreased at 4, 8, and 12 h from $H_2O_2-induced$ oxidative stress in each extract. After 12 h, level of DNA damage recovery was similar to the negative control level. These results suggest that CL has potential antioxidant activity and a protective effect against oxidation-induced DNA damage, and the methanol extract of CL was the most effective.

Effects of Kimchi Lactic Acid Bacteria Lactobacillus sp. OPK2-59 with High GABA Producing Capacity on Liver Function Improvement (GABA 생성능 우수 김치 젖산균 Lactobacillus sp. OPK2-59의 간 기능 개선 효과)

  • Bae, Mi-Ok;Kim, Hye-Jin;Cha, Youn-Soo;Lee, Myung-Ki;Oh, Suk-Heung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.11
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    • pp.1499-1505
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    • 2009
  • This study investigated the effect of improved liver function in rats administered with ethanol by kimchi lactic acid bacteria with high GABA producing capacity. Sprague-Dawley male rats were divided into four groups; normal diet control (NC), ethanol control (EC), ethanol+Lactobacillus sp. OPK2-59 normal powder (EL1), ethanol+Lactobacillus sp. OPK2-59 GABA powder (EL2) and fed for 6 weeks. Analysis showed that there were no significant differences in body weight and feed consumption among the groups during the experimental period. Also, there were no significant differences in organ weight among the groups. The test results showed total cholesterol and triglyceride in the blood concentration that were increased by ethanol administration were significantly lowered in EL2 group. Liver triglyceride was also significantly lowered in the EL2 group compared with the EC group. Serum GOT and GPT, and liver GOT levels were significantly lower in the EL2 group compared with the EC group. Serum ethanol concentration was lower in the EL1 and EL2 groups compared with the EC group. SOD activities in liver were significantly increased in the EL1 and EL2 groups compared with the EC group. These results suggest that Lactobacillus sp. OPK2-59 GABA powder improves lipid and enzyme profiles of rats administered with ethanol.

Exogenous proline mitigates the detrimental effects of saline and alkaline stresses in Leymus chinensis (Trin.)

  • Sun, Yan-Lin;Hong, Soon-Kwan
    • Journal of Plant Biotechnology
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    • v.37 no.4
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    • pp.529-538
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    • 2010
  • Proline accumulates in plants under environmental stresses including saline stress and alkaline stress. Here, we investigated the responses to two different stresses, saline stress (200 mM NaCl) and alkaline stress (100 mM $Na_2CO_3$) in two Leymus chinensis (Trin.) genotypes, LcWT07 and LcJS0107, and effects of exogenous proline on the activities of antioxidant enzymes. Both saline stress and alkaline stress significantly induced the accumulation of proline in leaves of the two genotypes after 96 h, and alkaline stress caused a transient and significant increase in LcJS0107 plants at 6 h. A reduction in the activities of catalase (CAT, EC 1.11.1.6) and ascorbate peroxidase (APX, EC 1.11.1.11), but not in the activity of superoxide dismutase (SOD, EC 1.15.1.1), was detected in plants exposed to saline and alkaline stresses. Remarkable decrease in relative water contents (RWC) was found in 144 h stressed plants. However, lipid peroxidation estimated by malonyldialdehyde (MDA) content in leaves remained relatively stable. With the addition of exogenous proline, it did not cause changes of proline levels in two genotypes, but combined with saline or alkaline stress, the exogenous application of proline significantly induced proline accumulation after even short treatment periods. Combined with salt stress, the exogenous application also increased the activities of CAT and APX. These results indicated that exogenous proline not only increases proline levels in vivo as a osmotic adjustment under stress, but mitigates the detrimental effects of saline and alkaline stresses by increasing the activities of antioxidant enzymes.

Quality Characteristics and Evaluation of Physiological Activities of Moju Made with Hovenia dulcis Thunb. (헛개나무를 이용한 모주의 품질 특성 및 생리활성(in vivo) 효능 검증)

  • Park, Yeon-Hee;Yu, Ok-Kyeong;Bae, Cho-Rong;Cha, Youn-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.11
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    • pp.1599-1606
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    • 2015
  • The purpose of this study was to assess the quality characteristics of Moju made with Hovenia dulcis Thunb. and its physiological effects on ICR mice. According to the sensory score, we selected Moju made with 1% Hovenia dulcis Thunb. among Moju made with 0, 0.5, 1, 5, and 10% Hovenia dulcis Thunb. Compared to Moju made without Hovenia dulcis Thunb., Moju made with 1% Hovenia dulcis Thunb. had higher proportions of moisture (86.77 g/100 g) and carbohydrates (11.86 g/100 g). The mean values of the physicochemical analyses were as follows: pH 4.91, acidity 0.28, $^{\circ}Brix$ 12.63, reducing sugar 68.97, alcohol content 0.1, alcohol density 0.998. Moju made with 1% Hovenia dulcis Thunb. did not have effects on DPPH radical scavenging activity; however, superoxide dismutase activity was significantly higher than that of Moju made without Hovenia dulcis Thunb. For assessing physiological activities, 4-week-old male ICR mice were divided into six groups (n=10): normal control group (NC), ethanol-administered group (EC), EC plus low-dose Moju made with 0% Hovenia dulcis Thunb. (MCL), EC plus high-dose Moju made with 0% Hovenia dulcis Thunb. (MCH), EC plus low-dose Moju made with 1% Hovenia dulcis Thunb. (MDL), and EC plus high-dose Moju made with 1% Hovenia dulcis Thunb. (MDH). Serum triglyceride (TG) level was reduced by 11.17% and 19.61% in the MDL and MDH groups, respectively, compared to the EC group. Serum total-cholesterol levels of MDL and MDH groups were significantly lower as compared to the EC group. Serum high-density lipoprotein-cholesterol levels of the MDL and MDH groups were significantly higher than those of the EC group. Liver TG levels were significantly reduced in the MCL and MDL groups. From these results, Moju made with Hovenia dulcis Thunb. demonstrated antioxidant activity and reduction of hyperlipidemia markers. Therefore, Moju made with Hovenia dulcis Thunb. can serve as a non-alcoholic beverage and functional food source.

A Study on Superoxide Dismutase from various Tissue of the Tricarboxylic acid cycle blocked Rat (Tricarboxylic acid회로를 차단한 흰쥐의 조직에서 Superoxide Dismutase에 관한 연구)

  • Kim, Yil
    • Korean Journal of Microbiology
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    • v.23 no.1
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    • pp.69-76
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    • 1985
  • This study was carried out to observe the formation of superoxide radicals and the changes in the activities of superoxide dismutase (EC.1.15.1.1.) from the various organs of a rat which was blocked tricarboxylic acid cycle. In order to block the tricarboxylic acid cycle, the beta-fluoroethylacetate was injected into peritoneal cavity of rat and removed the various tissues from the rat at internals of an hour. By tissue extracts being prepared by the method of Weigiger and Fridovich the activities of superoxide dismutase, aconitase, and contents of bliid glucose, citrates, and wuperoxide radicals were determined. The experimental results are summarized as follows: Accumulation of citrates if increased within three hours after treatment in the all tested tissues, especially, in the geart and spleen they are higher than one of other tissues as 12 and 20 times of control. The activities of aconitase are ingibited to 30-35% on an hour after beta-fluoroethylacetate treatment comparing with that of control rat. The content of blood glucose is increased to 1.6 fold of normal value after 5 hours of treatment. In all tested tissues, superoxide radicals are formed in the heart as 0.26 micromoles per gram tissue between one and three hours after treatment. The activities of total superoxide dismutase are increased between one and three hours after treatment in the all tested tissues and one of these enzymes in heart is highest. The activities of superoxide dismutase containing Mn are also increased with an increase of total superoxide dismutase activities.

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