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Signal Transduction of Eel Luteinizing Hormone Receptor (eelLHR) and Follicle Stimulating Hormone Receptor (eelFSHR) by Recombinant Equine Chorionic Gonadotropin (rec-eCG) and Native eCG

  • Byambaragchaa, Munkhzaya;Lee, So-Yun;Kim, Dae-Jung;Kang, Myung-Hwa;Min, Kwan-Sik
    • Development and Reproduction
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    • v.22 no.1
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    • pp.55-64
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    • 2018
  • Previous studies showed that recombinant equine chorionic gonadotropin ($rec-eCG{\beta}/{\alpha}$) exhibits both follicle-stimulating hormone (FSH) and luteinizing hormone (LH)-like activities in rat LHR- and FSHR-expressing cells. In this study, we analyzed signal transduction by eelFSHR and eelLHR upon stimulation with $rec-eCG{\beta}/{\alpha}$ and native eCG. The cyclic adenosine monophosphate (cAMP) stimulation in CHO-K1 cells expressing eelLHR was determined upon exposure to different doses (0-1,450 ng/mL) of $rec-eCG{\beta}/{\alpha}$ and native eCG. The $EC_{50$ values of $rec-eCG{\beta}/{\alpha}$ and native eCG were 172.4 and 786.6 ng/mL, respectively. The activity of $rec-eCG{\beta}/{\alpha}$ was higher than that of native eCG. However, signal transduction in the CHO PathHunter Parental cells expressing eelFSHR was not enhanced by stimulation with both agonist $rec-eCG{\beta}/{\alpha}$ and native eCG. We concluded that $rec-eCG{\beta}/{\alpha}$ and native eCG were completely active in cells expressing eelLHR, similar to the activity in the mammalian cells expressing LHRs. However, $rec-eCG{\beta}/{\alpha}$ and native eCG did not invoke any signaling response in the cells expressing eelFSHR. These results suggest that eCG has a potent activity in cells expressing eelLHR. Thus, we also suggest that $rec-eCG{\beta}/{\alpha}$ can induce eel maturation by administering gonadotropic reagents (LH), such as salmon pituitary extract.

Growth curve estimates for wither height, hip height, and body length of Hanwoo steers (Bos taurus coreanae)

  • Park, Hu-Rak;Eum, Seung-Hoon;Roh, Seung-Hee;Sun, Du-Won;Seo, Jakyeom;Cho, Seong-Keun;Lee, Jung-Gyu;Kim, Byeong-Woo
    • Korean Journal of Agricultural Science
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    • v.44 no.3
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    • pp.384-391
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    • 2017
  • Growth curves in Hanwoo steers were estimated by Gompertz, Von Bertalanffy, Logistic, and Brody nonlinear models using growth data collected by the Hanwoo Improvement Center from a total of 6,973 Hanwoo (Bos taurus coreanae) steers 6 to 24 months old that were born between 1996 and 2015. The data included three parameters: A, mature size of body measurement; b, growth ratio; and, k, intrinsic growth rate. Nonlinear regression equations for wither height according to Gompertz, Von Bertalanffy, Logistic, and Brody models were $Y_t=144.7e^{-0.5869e^{-0.00301t}}$, $Y_t=145.3(1-0.1816e^{-0.00284t})^3$, $Y_t=143.1(1+0.7356e^{-0.00352t})^{-1}$, and $Y_t=146.8(1+0.4700e^{-0.00249t})^1$, respectively, while those for hip height were $Y_t=144.5e^{-0.5549e^{-0.00312t}}$, $Y_t=145.0(1-0.1724e^{-0.00295t})^3$, $Y_t=143.1(1+0.6863e^{-0.00360t})^{-1}$, and $Y_t=146.2(1+0.4501e^{-0.00263t})^1$, respectively. Equations for body length $Y_t=174.1e^{-0.8342e^{-0.00289t}}$, $Y_t=175.8(1-0.2500e^{-0.00265t})^3$, $Y_t=170.0(1+1.1548e^{-0.00363t})^{-1}$, and $Y_t=180.3(1+0.6077e^{-0.00215t})^1$, respectively, for the same models. Among the four models, the Brody model resulted in the lowest mean square error, with mean square errors of 31.79, 30.57, and 42.13, respectively, for wither height, hip height, and body length. Also, an estimated birth wither height, birth hip height, and birth body length (77.98, 80.57, and 70.97 cm, respectively) were lower in the Brody model than in other models. An inflection point was not observed during the growth phase of Hanwoo steer according to the growth curves calculated using Gompertz, Von Bertalanffy, and Logistic models. Based on the results, we concluded that the regression equation using the Brody model was the most appropriate among the four growth models. To obtain more accurate parameters, however, using data from a wider production period (from birth to shipping) would be required, and the development of a suitable model for body conformation traits would be needed.

Inhibition of Cervical Cancer Cell Growth by Gene Silencing of HPV16 E6 Induced by Short-interfering RNA

  • Park, Sang-Muk;Lee, Sun-Kyung;Kim, Yoon-Sik
    • Korean Journal of Clinical Laboratory Science
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    • v.43 no.3
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    • pp.89-97
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    • 2011
  • The Human Papilloma Virus (HPV) infection has been strongly associated with pathogenesis of uterine cervix carcinoma. HPV type 16, a causative agent of uterine cervix carcinoma, encodes the E6 and E7 oncogenes, expression of which is pivotal for malignant transformation and maintenance of malignant phenotypes. To develop a gene therapy for HPV-related carcinoma, We investigated the effect of E6 short-interfering RNA (E6 siRNA) on the expression of this oncogene and on the growth of HPV 16-related uterine cervix carcinoma cells. SiHa cells, a uterine cervix carcinoma cell line, which contain a single copy of HPV 16 integrated in the chromosome and express the E6 and E7 oncogenes. Before 24 hr of transfection, cells were seeded and transfected with control plasmid or E6 siRNA-expressing plasmid. The mRNA was analysed by reverse transcriptase polymerase chain reaction (RT-PCR). The cell growth rate was investigated by MTT method. The E6 mRNA level in SiHa cells was decreased in HPV 16 E6 siRNA-expression vector transfected cells and a decrease in the growth of these cells was also observed. From these results. it is evident that E6 siRNA played a role in suppression of growth of SiHa cells and has a fair chance as a candidate for gene specific therapy for HPV related uterine cervix carcinoma.

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Effects of Science high school students' Competency through Intensive Research and Education Program in KAIST (과학기술특성화대학(KAIST)을 중심으로한 R&E 집중연구가 과학고등학교 학생에게 미치는 영향)

  • Choi, Jinsu;Kim, Young Min;Lee, Young Ju
    • Journal of The Korean Association For Science Education
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    • v.39 no.6
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    • pp.729-738
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    • 2019
  • Research and Education(R&E) program has been emphasized for scientifically gifted students since it can improve scientific research abilities and problem solving abilities because it provides scientific research experiences with professional researchers. The purpose of this study is to investigate the effect of R&E program on creative leader competency and science career orientation. For this study, 62 science high school students participated from 78 science high school students participating in an R&E program. Pre and Post surveys of creative leader competency and science career orientation were administered to the students. The results showed that R&E program improved students' creative leader competency in intellectual and affective characteristic. However, there are no differences in science career orientation because science high school students already had high science career orientation. The implications of this study were discussed.

Double Mutations in eIF4E and eIFiso4E Confer Recessive Resistance to Chilli Veinal Mottle Virus in Pepper

  • Hwang, JeeNa;Li, Jinjie;Liu, Wing-Yee;An, Song-Ji;Cho, Hwajin;Her, Nam Han;Yeam, Inhwa;Kim, Dosun;Kang, Byoung-Cheorl
    • Molecules and Cells
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    • v.27 no.3
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    • pp.329-336
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    • 2009
  • To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinal mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum 'Dempsey' containing an elF4E mutation ($pvr1^2$) and C. annuum 'Perennial' containing an elFiso4E mutation (pvr6). C. annuum 'Dempsey' was susceptible and C. annuum 'Perennial' was resistant to ChiVMV. All $F_1$ plants showed resistance, and $F_2$ individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five $F_2$ and 329 $F_3$ plants of 17 families were genotyped with $pvr1^2$ and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both $pvr1^2$ and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in elF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of $F_2$ plants revealed that all plants containing homozygous genotypes of both $pvr1^2$ and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of elF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.

e-Science 국내외 기술 동향

  • Hwang, Sun-Uk;Nam, Deok-Yun;Kim, Beop-Gyun;Yeom, Heon-Yeong
    • Korea Information Processing Society Review
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    • v.15 no.2
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    • pp.43-57
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    • 2008
  • IT기술의 눈부신 발전으로 인해 과학적 연구(scientific research)는 더 이상 한 개인 연구자의 실험실내에서 수행되는 것이 아니라 학제, 실험실, 연구기관, 심지어는 국가를 포괄한 연구자 커뮤니티의 협업 형태로 수행되고 있다. e-Science란 이처럼 IT 기반으로 과학, 공학에 있어서 새로운 문제들을 풀기 위한 자원과 연구자들의 글로벌 협업을 말한다. 우리나라도 지난 2005년부터 국가적 차원에서 e-Science 사업을 진행해 오고 있다. 유럽도 지난 2004년부터 소위 EGEE (Enabling Grid for E-SciencE)라는 e-Science 프로젝트를 전 유럽차원에서 진행해 오고 있다. 본 논문에서는 그동안 진행해온 한국과 유럽의 e-Science 환경 구축에 있어서 핵심적인 역할을 하고 있는 e-Science 기반 기술에 대해서 소개를 하고자 한다.

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Alteration of Lipid Metabolism by Ginseng Supplements With Different Levels of Vitamin E in High Cholesterol-Fed Rats

  • Do, Kyung-Min;Park, Yong-Bok;Bok, Song-Hae;Lee, Mi-Kyung;Jeong, Tae-Sook;Choi, Myung-Sook
    • Preventive Nutrition and Food Science
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    • v.6 no.1
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    • pp.66-72
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    • 2001
  • Ginseng may have antioxidant and pharmacologic effects similar to those of vitamin E. The interactive effect of ginseng and vitamin E was studied with respect to cholesterol metabolism and the antioxidant status. A ginseng supplement (0.1%, wt/wt) with comparable levels of vitamin E was provided with a high-cholesterol (1%, wt/wt) diet to rats for 5 weeks. The amount of vitamin E included in the ginseng-free and ginseng diets was either a low (low-E) or a normal (normal-E) level. The ginseng supplements significantly (p<0.05) altered the concentrations of plasma triglycerides in both the low-vitamin E group and normal-vitamin E group compared to the each ginseng-free group. The hepatic triglyceride and cholesterol content were not significantly (p>0.05) different between groups regardless of the vitamin E level in the diet. The hepatic HMG-CoA reductase activity was significantly (p<0.05) lowered by the ginseng supplement in both the low-vitamin E and the normal-vitamin E groups compared to the ginseng-free group. The HMG-CoA reductase activity was also significantly (p<0.05) lowered with in increase of the dietary vitamin E in the ginseng-free group. The excretion of fecal neutral sterol was significantly (p<0.05) lower in the normal-E ginseng group than th low-E ginseng-free group. Neither dietary ginseng nor vitamin E significantly changed the hepatic antioxidant enzymes activity. This data indicates that ginseng supplements lower the concentration of plasma triglyceride and hepatic HMG-CoA reductase activity regardless of eh dietary vitamin E level. This information may contribute to understanding the interactive effect of ginseng and vitamin E on cholesterol biosynthesis in high cholesterol-fed rats.

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An Integrated Access Control for Sharing of E-Science Grid Resources (유휴 멀티 e-Science 그리드 자원 공유를 위한 통합 자원 접근 제어)

  • Jung, Im-Y.;Jung, Eun-Jin;Yeom, Heon-Y.
    • Journal of KIISE:Computer Systems and Theory
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    • v.35 no.9_10
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    • pp.452-465
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    • 2008
  • This paper proposes a light-weight, seamless integrated access control for global e-Science resource sharing. E-Science, based on Grid Computing, was designed to help scientists to remotely control and process the Grid resources such as high-end equipments and remote machines. As many researchers engage in the e-Science Grids, the researchers in a grid often have to wait for or give up use of the Grid resources, even when there are idle resources in other Grids. In this case, provided that proper compensation is given, Grid resource sharing is helpful both for the researchers and the Grids which provide their resources. But, sharing Grid resources globally is not simple, as each e-Science Grid is especially designed for resource sharing in its Virtual Organization(VO) and already has its unique access control policy for its resources. This paper proposes a new integrated access control for e-Science Grid resource sharing. The access control is light-weight without any priori service level agreement(SLA)s among the Grids which share their resources and seamless because the users can use the resources shared as the ones belonging to their Grids without their additional registration to the other Grids.

Human Keratin 14 Driven HPV 16 E6/E7 Transgenic Mice Exhibit Hyperkeratinosis

  • Kim, Sung-Hyun;Kim, Kil-Soo;Lee, Eun-Ju;Kim, Myoung-Ok;Park, Jun-Hong;Cho, Kyoung-In;Kazuhiko-Imakawa;Hyun, Byung-Hwa;Chang, Kyu-Tae;Lee, Hoon-Taek;Ryoo, Zae-Young
    • Proceedings of the KSAR Conference
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    • pp.215-215
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    • 2004
  • Human papillomavirus type 16 (HPV16) has been known as a major causative factor for the development of uterine cervical carcinomas. To investigate the in vivo activity of HPV16 expressed in squamous epithelia, transgenic mice harboring HPV16 E6/E7 with human keratin 14 (hK14) promoter were generated. Grossly, hK14 driven HPV16 E6/E7 transgenic mice exhibited multiple phenotypes, including wrinkled skin that was apparent prior to the appearance of hair in neonates, thickened ears, and loss of hair in adults. (omitted)

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A SCORM-based e-Learning Process Control Model and Its Modeling System

  • Kim, Hyun-Ah;Lee, Eun-Jung;Chun, Jun-Chul;Kim, Kwang-Hoon Pio
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.5 no.11
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    • pp.2121-2142
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    • 2011
  • In this paper, we propose an e-Learning process control model that aims to graphically describe and automatically generate the manifest of sequencing prerequisites in packaging SCORM's content aggregation models. In specifying the e-Learning activity sequencing, SCORM provides the concept of sequencing prerequisites to be manifested on each e-Learning activity of the corresponding tree-structured content organization model. However, the course developer is required to completely understand the SCORM's complicated sequencing prerequisites and other extensions. So, it is necessary to achieve an efficient way of packaging for the e-Learning content organization models. The e-Learning process control model proposed in this paper ought to be an impeccable solution for this problem. Consequently, this paper aims to realize a new concept of process-driven e-Learning content aggregating approach supporting the e-Learning process control model and to implement its e-Learning process modeling system graphically describing and automatically generating the SCORM's sequencing prerequisites. Eventually, the proposed model becomes a theoretical basis for implementing a SCORM-based e-Learning process management system satisfying the SCORM's sequencing prerequisite specifications. We strongly believe that the e-Learning process control model and its modeling system achieve convenient packaging in SCORM's content organization models and in implementing an e-Learning management system as well.