• Title, Summary, Keyword: Drosophila

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On Drosophil quelpartiensis sp.nov. With Supplementary Notes on the Female of Drosophila pseudonokogiri Kang, Lee and Bahng (초파리의 일신종과 Drosophila pseudonokogiri의 암컷에 대하여)

  • 강영선
    • The Korean Journal of Zoology
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    • v.10 no.2
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    • pp.13-14
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    • 1967
  • Drosophila 속의 Dichaetophora 아속은 1940년 Duda 에 의하여 설정된 후 Duda, Burla 등에 의하여 4 종이 기록되어 있고, 동양에서는 하기의 3 종이 기재되어 있다. 즉 Drosophila raridentata Okada & Chung, 1960(분포 : 한국·일본) Drosophila magnidentata Lee, 1964(분포 : 한국) Drosohphila surukella Okada, 1965(분포 ; Okinawa) 저자들은 제주도 한수봉에서 채집된 정리분류한 결과 Dichaetophora 아속의 1 신종을 얻었기에 이에 기재하였다. 이 밖에 저자들이 1965년에 발표한 Drosophila pseudonokogiri 의 암컷에 대하여 기재하였다.

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Two New Species of the Genera Microdrosophila and Drosophila (Drosophilidae) from Korea

  • Kim, Nam-Woo;Joo, Eun-Young
    • Animal cells and systems
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    • v.6 no.2
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    • pp.85-87
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    • 2002
  • Two new species belonging to the genera Microdrosophila and Drosophila, collected from Daegwallyeong and Mt. Halla in Korea are described with illustrations of the male genitalia. Microdrosophila (Microdrosophila) gangwonensis n. sp. seems close to M. (M.) purpurata Okada, 1956, but the new species has different phallic organ. Drosophila (Drosophila) taekjuni is close to D. (D.) brevitabula Zhang and Toda, 1992, in form, but differs in the shape of aedeagus. Eight species of the genus Microdrosophila and five species in the D. immigrans species-group of genus Drosophila, including the new species are known to be distributed in Korea.

Comparative analyses of susceptibility to chemicals associated with fermentation between Drosophila melanogaster and Drosophila suzukii

  • KIM, YiSeul;LEE, Sungho;KIM, Yeong Ho;KIM, Young Ho
    • Entomological research
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    • v.48 no.6
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    • pp.514-521
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    • 2018
  • Drosophila melanogaster Meigen and Drosophila suzukii Matsumura are taxonomically close Drosophila species belonging to the family Drosophilidae and melanogaster group. D. melanogaster is thought to be evolutionarily adapted to overripe, decaying, and fermented fruits, in which large amounts of chemicals such as ethanol, acetic acid, and 2-phenylethanol are produced, whereas, D. suzukii is attracted to fresh ripening fruit. Considering the distinct habitats of the two flies, D. suzukii is hypothesized to exhibit higher susceptibility to these chemicals than D. melanogaster. Therefore, in this study, we investigated the survival rate of the flies at various concentrations of three chemicals (2-phenlyethanol, acetic acid, and ethanol) and calculated the lethal concentration (LC) values to compare the tolerance and susceptibility of D. melanogaster and D. suzukii to the chemicals. Our results revealed that D. melanogaster exhibited higher tolerance than D. suzukii to all chemicals, supporting the hypothesis of different evolutionary adaptations to distinct habitats of the two flies.

Drosophila blood as a model system for stress sensing mechanisms

  • Shim, Jiwon
    • BMB Reports
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    • v.48 no.4
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    • pp.223-228
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    • 2015
  • The Drosophila lymph gland is the hematopoietic organ in which stem-like progenitors proliferate and give rise to myeloid-type blood cells. Mechanisms involved in Drosophila hematopoiesis are well established and known to be conserved in the vertebrate system. Recent studies in Drosophila lymph gland have provided novel insights into how external and internal stresses integrate into blood progenitor maintenance mechanisms and the control of blood cell fate decision. In this review, I will introduce a developmental overview of the Drosophila hematopoietic system, and recent understandings of how the system uses developmental signals not only for hematopoiesis but also as sensors for stress and environmental changes to elicit necessary blood responses. [BMB Reports 2015; 48(4): 223-228]

Analysis of Two Promoters that Control the Expression of the GTP cyclohydrolase I Gene in Drosophila melanogaster

  • Byun, Jaegoo;Yoon, Jaeseung;Baek, Kwanghee
    • Molecules and Cells
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    • v.27 no.5
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    • pp.583-589
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    • 2009
  • GTP cyclohydrolase I (GTPCH) is a key enzyme in the de novo synthesis of tetrahydrobiopterin. Previously, the Drosophila melanogaster GTPCH gene has been shown to be expressed from two different promoters (P1 and P2). In our study, the 5'-flanking DNA regions required for P1 and P2 promoter activities were characterized using transient expression assay. The DNA regions between -98 and +31, and between -73 and +35 are required for efficient P1 and P2 promoter activities, respectively. The regions between -98 and -56 and between -73 and -41 may contain critical elements required for the expression of GTPCH in Drosophila. By aligning the nucleotide sequences in the P1 and P2 promoter regions of the Drosophila melanogaster and Drosophila virilrs GTPCH genes, several conserved elements including palindromic sequences in the regions critical for P1 and P2 promoter activities were identified. Western blot analysis of transgenic flies transformed using P1 or P2 promoter-lacZ fusion plasmids further revealed that P1 promoter expression is restricted to the late pupae and adult developmental stages but that the P2 promoter driven expression of GTPCH is constitutive throughout fly development. In addition, X-gal staining of the embryos and imaginal discs of transgenic flies suggests that the P2 promoter is active from stage 13 of embryo and is generally active in most regions of the imaginal discs at the larval stages.

Drosophila as a model for unfolded protein response research

  • Ryoo, Hyung Don
    • BMB Reports
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    • v.48 no.8
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    • pp.445-453
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    • 2015
  • Endoplasmic Reticulum (ER) is an organelle where most secretory and membrane proteins are synthesized, folded, and undergo further maturation. As numerous conditions can perturb such ER function, eukaryotic cells are equipped with responsive signaling pathways, widely referred to as the Unfolded Protein Response (UPR). Chronic conditions of ER stress that cannot be fully resolved by UPR, or conditions that impair UPR signaling itself, are associated with many metabolic and degenerative diseases. In recent years, Drosophila has been actively employed to study such connections between UPR and disease. Notably, the UPR pathways are largely conserved between Drosophila and humans, and the mediating genes are essential for development in both organisms, indicating their requirement to resolve inherent stress. By now, many Drosophila mutations are known to impose stress in the ER, and a number of these appear similar to those that underlie human diseases. In addition, studies have employed the strategy of overexpressing human mutations in Drosophila tissues to perform genetic modifier screens. The fact that the basic UPR pathways are conserved, together with the availability of many human disease models in this organism, makes Drosophila a powerful tool for studying human disease mechanisms. [BMB Reports 2015; 48(8): 445-453]

Transient Receptor Potential Ion Channels and Animal Sensation: Lessons from Drosophila Functional Research

  • Kim, Chang-Soo
    • BMB Reports
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    • v.37 no.1
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    • pp.114-121
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    • 2004
  • Ion channels of the transient receptor potential (TRP) superfamily are non-selective cationic channels with six transmembrane domains. The TRP channel made its first debut as a light-gated $Ca^{2+}$ channel in Drosophila. Recently, research on animal sensation in Drosophila disclosed other members of the TRP family that are required for touch sensation and hearing as well as the sensation of painful stimuli.

On a New Species, "Drosophila Trilineata" sp. nov. (한국산초파리 일신종에 대하여)

  • 정용재
    • The Korean Journal of Zoology
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    • v.3 no.2
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    • pp.9-12
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    • 1960
  • Drosophila (Drosophila) trilineata sp.nov. : 국명 세줄등초파리 . 1. 본 종은 1958년 8월 6일에 울릉도의 성인봉에서 그리고 1958년 9월 5일 광릉에서 채집된바, 외형이 Drosophila trivittata Strobl 과 혹사하기에 비종으로 잘못 동정발표하였던 것이다. 연이나 1959년 10월 영국 Edinburgh 대학의 E.B. Basden이 이 본종의 표본을 관찰한 다음 저자가 채집한 상기종은 Strobl 종이 아니고 신종인 듯 생각한다는 암시를 받았기에 그 후세밀히 조사한 바 신종으로 신정할 수있었기에 여기서 발표하는 바이다. 2. 본종은 외형 특히 중배흉부의 stripe의 모양으로 보아 Drosophila trivittata Strobl 과 혹사하나 무미 plate 의 측부에 뚜렷한 knob가 있다는 점, genital arch와 clasper의 기리의 관계, phallic organ 의 형태, egg-guide 의 강모등에 뚜렷한 차이가 있다. 본종은 명백한 fungus feeder 로서 laboratory breeding 은 불가능이었다.

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Upstream paths for Hippo signaling in Drosophila organ development

  • Choi, Kwang-Wook
    • BMB Reports
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    • v.51 no.3
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    • pp.134-142
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    • 2018
  • Organ growth is fundamental to animal development. One of major mechanisms for growth control is mediated by the conserved Hippo signaling pathway initially identified in Drosophila. The core of this pathway in Drosophila consists of a cascade of protein kinases Hippo and Warts that negatively regulate transcriptional coactivator Yorkie (Yki). Activation of Yki promotes cell survival and proliferation to induce organ growth. A key issue in Hippo signaling is to understand how core kinase cascade is activated. Activation of Hippo kinase cascade is regulated in the upstream by at least two transmembrane proteins Crumbs and Fat that act in parallel. These membrane proteins interact with additional factors such as FERM-domain proteins Expanded and Merlin to modulate subcellular localization and function of the Hippo kinase cascade. Hippo signaling is also influenced by cytoskeletal networks and cell tension in epithelia of developing organs. These upstream events in the regulation of Hippo signaling are only partially understood. This review focuses on our current understanding of some upstream processes involved in Hippo signaling in developing Drosophila organs.

Expression of Enhanced Green Fluorescent Protein from Stably Transformed Drosophila melanogaster S2 Cells

  • Lee, Jong-Min;Park, Jong-Hwa;Chung, In-Sik
    • Journal of Microbiology and Biotechnology
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    • v.10 no.1
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    • pp.115-118
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    • 2000
  • Recombinant plasmids harboring a heterologous gene coding for the enhanced green fluorescent protein (EGFP) were transfected and expressed in Drosophila melanogaster S2 cells. A stable transformation of polyclonal cell populations expressing EGFP were isolated after 4 weeks of selection with hygromycin B. The recombinant EFGP expressed in transformed S2 cells consisted of a molecular weight of 27 kDa. EGFP expression was also confirmed by fluorometric measurement. The maximum EGFP concentration was about 9.3 mg/I. The present findings demonstrate not only the successful stable expression of EGFP in Drosophuila was about 9.3 mgI. The present findings demonstrate not only the successful stable expression of EGFP in Drosophila S2 cells, but also the use of EGFP as a reporter to analyze gene expression, with its potential of a Drosophila cell expression system for recombinant protein production being an alternative to a baculovirus-insect cell expression system.

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