• Title, Summary, Keyword: Dithiothreitol

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Protective Effects of Chemical Drugs on the Course of Uranium-induced Acute Renal Failure (우라늄오염에 의한 신부전증에 미치는 제염제의 방호효과)

  • Kim, Tae-Hwan;Chung, In-Yong;Kim, Sung-Ho;Kim, Kyeng-Jung;Bang, Hyo-Chang;Yoo, Seong-Yul;Chin, Soo-Yil
    • Journal of Radiation Protection and Research
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    • v.15 no.2
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    • pp.27-39
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    • 1990
  • Appreciable radiation exposures certainly were occurred in the reactor burn-up, the nuelear fall-out and the surroundings of nuclear installations with radioactive effluents. Therefore, radioactive nuclides is not only potentially hazardous to workers of nuclear power plants and related industrials, but also the wokers who handle radioactive nuclides in biochemical research and nuclear medicine diagnostics. And in the case of occurring the nuclear accidents, the early medical treatment of radiation injury should be necessary but little is established medical procedures to decontaminate the victims of internal contamination of radioactive nuclides in korea. Accordingly, to achieve the basic data for protective roles and medical treatment of radiation injury, the present studies were carrid out to evaluate the decontamination of uranium by the chemical drugs. The results observed were summarized as follows: 1. The combined treatmet group of sodium bicarbonate and saline with uranyl nitrate injection simultaneously and the dithiothreitol group that was administered 30 minutes after uranyl nitrate injection were increased significantly in the change of body weight than uranyl nitrate-only group (P<0.005). 2. All the experimental groups were increased the fluid intake and urine volume on the uranyl nitrate-induced acute renal failure. but the combined treatment group of sodium bicarbonate and saline with uranyl nitrate injection simultaneously and the dithiothreitol group that was administered 30 minutes after uranyl nitrate injection have the higher increment of fluid intake and urine volume (P<0.05). 3. When sodium bicarbonate and saline was treated with uranyl nitrate injection simultaneously. and dithiothreitol was administered 30 minutes after uranyl nitrate injection. there was significantly reduced in BUN concentration (P<0.01). 4. When dithiothreitol was administered 30 minutes after uranyl nitrate injection. there was reduced more significantly on the increment of serum creatinine concentration than that observed in uranyl nitrate-only group(P<0.01). but when the combined treatment of sodium bicarbonate and saline with uranyl nitrate simultaneously, there was still. albeit much less marked. decrease in serum creatinine concentration. 5. The sodium bicarbonate and saline was treated with uranyl nitrate simultaneously and dithiothreitol was administered at 30 minutes after uranyl nitrate were excreted markedly higher urine creatinine concentration than the uranyl nitrate-only group. 6. Uranyl nitrate has been used in experimental animals to produce hydropic degeneration and swelling of proximal tubules, disappearance of microvilli and brush border or necrosis in the kidney and centrilobular necrosis, congestion, and telangiectasia of the liver. When the sodium bicarbonate and saline was treated with uranyl nitrate simultaneously, and dithiothreitol was administered. 30 minutes after uranyl nitrate, there was more marked the protective effect than uranyl nitrate-only group. Finally, if the sodium bicarbonate and saline may administered as quickly as possible each time that some risk for internal contamination, with uranium, and dithiothreitol is administered 30 minutes after uranium contamination, there ameliorates the course of uranyl nitrate-induced acute renal failure.and this effect is assocciated with prevention of uranium (heavy metal)-induced alterations in BUN, serum creatinine, urine creatinine, fluid intake, urine volume and body weight.

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Dithiothreitol Attenuates the Pathogenic Interaction Between Pseudomonas aeruginosa and Drosophila melanogaster

  • Lee, Ji-Sun;Kim, Seol-Hee;Cho, You-Hee
    • Journal of Microbiology and Biotechnology
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    • v.14 no.2
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    • pp.367-372
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    • 2004
  • Infection of Drosophila melanogaster adults with Pseudomonas aeruginosa (PA14) can kill the flies within 48h. We found that the virulence of PA14 was significantly attenuated when treated with a reducing agent, dithiothreitol (DTT), prior to infection. Infection with DTT-treated PA14 elevated Metchnikowin expression at 22 h post-infection and the virulence of DTT-treated PA14 was not attenuated in Dif and Relish mutants. These results suggest that DTT pre-treatment of PA14 can aggravate certain virulence factors that may be required to paralyze fly immune responses, triggering Metchnikowin expression via Dif and Relish activations.

Multiplexed DNA Detection with DNA-Functionalized Silver and Silver/Gold Nanoparticle Superstructure Probes

  • Kim, Ji-Young;Lee, Jae-Seung
    • Bulletin of the Korean Chemical Society
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    • v.33 no.1
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    • pp.221-226
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    • 2012
  • DNA-functionalized silver and silver/gold bimetallic nanoparticle superstructure probes with controllable sizes and optical properties are synthesized using monothiol DNA and dithiothreitol. The superstructures exhibit a very narrow size distribution, which can be easily controlled by balancing the ratio of dithiothreitol and DNA. These superstructures assemble reversibly in a highly cooperative manner, and are SERS active. Multiplexed colorimetric detection of DNA targets using these superstructure probes has been demonstrated to identify three different DNA target sequences that are associated with three lethal diseases, respectively.

Thermostability of Polyphenol Oxidase from Potato (Solanum tuberosum L.) (감자 Polyphenol Oxidase의 열안정성)

  • 김나영;이민경;박인식;방극승;김석환
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.844-847
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    • 2001
  • Factors affecting thermostability of polyphenol oxidase (PPO) from potato were studied for the purpose of providing useful information for food processing operations. The enzyme was most stable at pH 7.0 and it was inhibited to 70% after heat treatment at 8$0^{\circ}C$ for 1 min. The z-value for the thermal inactivation of the PPO was 12.17$\pm$0.58$^{\circ}C$. The thermostability of the enzyme was reduced by addition of sodium chloride. And the activity was inhibited by addition of reducing reagents such as 2-mercaptoethanol and dithiothreitol.

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Enzymatic Determination of Glucose Using Soybean Sprouts Peroxidase. (콩나물 Peroxidase를 이용한 포도당의 효소적 분석)

  • 이민경
    • Journal of Life Science
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    • v.8 no.4
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    • pp.416-420
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    • 1998
  • Soybean sprouts peroxidase can be used for enzymatic determination of glucose. Peroxidase from soybean sprouts was purified by ammonium sulfate precipitation and DEAE Sephacel column chromatography. The glucose could be quantitatively assayed by using glucose oxidase and soybean sprouts peroxidase. The optimum pH and temperature for glucose assay were of pH 5.5 and $40^{\circ}C$, respectively. The relationship between absorbance and glucose concentration was linear. And also the relationship between absorbance and reaction time was linear. The reducing agents such as L-cysteine, dithiothreitol inhibited the glucose assay by glucose oxidase and soybean sprouts peroxidase.

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Retrospective dosimetry using fingernail electron paramagnetic resonance response

  • Noori, Abbas;Mostajaboddavati, Mojtaba;Ziaie, Farhood
    • Nuclear Engineering and Technology
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    • v.50 no.3
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    • pp.526-530
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    • 2018
  • Human fingernails were used to estimate the radiation dose via electron paramagnetic resonance measurements of radiation-induced radicals. The limiting factors in this research were mechanically induced electron paramagnetic resonance signals due to the mechanical stress during the preparation of the samples. Therefore, different treatment methods of fingernails were used to reduce the mechanically induced signals. The results demonstrate that the mechanically induced and radiation-induced signals have apparently different microwave power saturation behaviors. In addition, the mechanically induced signal shows a fading evolution over time and reaches a constant value. Chemical treatment using the different reagents showed that the minimum mechanically induced signal was obtained using the dithiothreitol reagent. The dose-response curves of the samples treated with dithiothreitol for 30 minutes demonstrated a greater linearity than those of samples treated for 5 minutes. Therefore, to find an unknown absorbed dose in a fingernail sample using a calibration curve, we recommend adopting the mentioned chemical treatment procedure to reduce the uncertainty.

Effects of Magnetic Pole on Orientation of Bull Sperm Treated by Dithiothreitol or Heparin

  • Pham, Du Ngoc;Shinjo, Akihisa;Kurnianto, Edy
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.2
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    • pp.155-162
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    • 2001
  • Effect of the north (N) or south pole (S) of a magnet and dithiothreitol (DTT) or heparin sodium (H) on the orientation of bull sperm were studied. Sperm were collected from four Japanese Black bulls of Okinawa Prefectural Livestock Experimental Station were treated with various concentrations of DTT or H during the first and sixth days and were then exposed them to N or S with a magnetic field of 3,000 Gauss for 24 hours. Experimental results showed that both N and S significantly increased the perpendicular oriented percentage of bull sperm in most treatments not treated or treated with DTT or H. Anisotropy of magnetic field of bull sperm was the main cause which induced them to orient perpendicular to the magnetic direction. The results of the experiment also showed that, in most cases, the two polarities had the same effect on the orientation of bull sperm. However, in some cases, N exerted a stronger effect on the perpendicular percentage of bull sperm than S, such as with the 20 mM DTT treatment at the first and sixth days and the 100 units H treatment at the sixth day. These cases showed that the perpendicular oriented percentage of bull sperm at N was significantly higher than that of S. It was only the 100 units heparin treatment at S both at the first and sixth days that upward perpendicular orientation was higher than that at N. This might be due to the fact that protamin-DNA structure of a small number of bull sperm nuclei slightly changed because of heparin action.

Reduction of Radiation-induced Chromosome Aberration and Apoptosis by Dithiothreitol

  • Kim, Jeong Hee;Lee, Eun Ju;Hyun, Jin Won;Kim, Sung Ho;Mar, Woongchon;Kim, Jin Kyu
    • Archives of Pharmacal Research
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    • v.21 no.6
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    • pp.683-687
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    • 1998
  • We have examined in vitro and in vivo radioprotective effects of a well-known thiol-containing compound, dithiothreitol (DTT). The treatment of both 0.5 and 1mM of DTT significantly increased clonogenic survival of ${\gamma}$-ray irradiated Chinese hamster (V79-4) cells. In order to investigate the possible radioprotective mechanism of DTT, we measured gamma-ray induced chromosome aberration by micronucleus assay. In the presence of 0.5mM or 1mM DTT, the frequencies of micronuclei were greatly reduced in all dose range examined (1.5-8 GY). Slightly higher reduction in micronucleus formation was observed in 1mM DTT-treated cells than in 0.5mM DTT-treated cells. In addition, incubation with both 0.5 and 1mM of DTT prior to gamma-ray irradiation reduced nucleosomal DNA fragmentation at about same extent, this result suggests that treatment of DTT at concentrations of 0.5 and 1mM reduced radiation-induced apoptosis. In vivo experiments, we also observed that DTT treatment reduced the incidence of apoptotic cells in mouse small intestine crypts. In irradiated control group 4.4${\pm}$0.5 apoptotic cells per crypt were observed. In DTT-administered and irradiated mice, only 2.1${\pm}$0.4 apoptotic cells per crypt was observed. In vitro and in vivo data obtained in this study showed that DTT reduced radiation-induced damages and it seems that the possible radioprotective mechanisms of action of DTT are prevention of chromosome aberration.

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Synthetic Regulatory Elements of the Nopaline Synthase Promoter in Higher Plants (고등 식물에서 Nopaline Synthase Promoter의 합성 조절 요소)

  • Kim, Young-Hee
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.4
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    • pp.201-205
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    • 1995
  • The synthetic oligomers called nos right palindrome (RP) element and left palindrome (LP) element were inserted into nos.minimal promoter nos 5'-101 deletion mutant The activity of nos promoter was measured by studying the expression pattern of gene fusion between nos promoter and reporter genes such as chloramphenicol acetyltransferase and $\beta$-glucuconidase. Analysis of transgenic tobacco plane carrying transgene showed that the activity of nos minimal promoter activity was recovered by insertion of synthetic nos RP element. Nos RP element insertion of nos minimal promoter was induced by auxin, dithiothreitol, salicylic acid and methyl jasmonate.

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Reduction of Nitrotoluenes and Simultaneous Removal of Hydrogen Sulfide and Nitrotoluenes by Co3+-centered Hematoporphyrin (포피린의 촉매작용에 의한 니트로톨루엔의 환원 및 니트로톨루엔과 황화수소의 동시 제거)

  • Cho, Jeong-Guk;Kang, An-Soo
    • Applied Chemistry for Engineering
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    • v.5 no.1
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    • pp.37-43
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    • 1994
  • Mononitrotoluenes were reduced to aminotoluenes using porphyrin as a catalyst in the presence of several types of reductants including hydrogen sulfide and 1, 4-dithiothreitol(DTT). Intermediates and final products of porphyrin-catalyzed reduction of mononitrotoluenes were identified and a pathway for the reduction of the nitro group to the corresponding amino group was proposed. The optimum pH for the reduction was determined. The catalytic activity of the porphyrin was confirmed by UV/VIS absorption spectra and basic kinetics of porphyrin-catalyzed reduction were studied. Of several types of reductants tested, DTT sodium hydrosulfite, and hydrogen sulfide were seen to give significant reduction of nitrobodies. When hydrogen sulfide was used as a reductant hydrogen sulfide and nitrotoluenes were removed simultaneously.

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