• Title, Summary, Keyword: DPPH

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Effects of Alismatis Rhizoma Pharmacopuncture Extracts on the Elastase Activity and DPPH and NO Scavenging Activities (택사(澤瀉) 약침액(藥鍼液)의 elastase 활성과 DPPH, NO 소거능에 미치는 영향)

  • Lee, Se-Na;Kim, Myung-Gyou;Kim, Myoung-Hee;Kim, Hyung-Jun;Jo, Hak-Jun;Kim, Ee-Hwa;Leem, Kang-Hyun
    • Korean Journal of Acupuncture
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    • v.28 no.1
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    • pp.15-22
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    • 2011
  • 엘라스타제는 폐의 탄성 섬유를 파괴하여 폐기종과 폐고혈압을 유발하는 역할을 한다. 이에 본 연구는 택사(澤瀉) 약침액(藥鍼液)의 엘라스타제 억제효능과 항산화효능을 측정하여 폐기종과 폐고혈압 치료의 가능성을 확인하고자 실시 하였다. 방법 : 택사(澤瀉) 약침액(藥鍼液)의 엘라스타제에 대한 억제효능과 di(phenyl)-(2,4,6-trinitrophenyl) iminoazanium (DPPH) 및 nitric oxide (NO) 자유기 소거능을 측정하였다. 결과 : 택사(澤瀉) 약침액(藥鍼液)은 엘라스타제 활성을 통계적으로 유의하게 억제하였고, DPPH 및 NO 자유기 소거능이 있음이 통계적으로 유의하게 확인되었다. 결론 : 본 연구를 통해 택사(澤瀉) 약침액(藥鍼液)의 엘라스타제 억제효능과 항산화 효능이 확인되었다. 본 연구 결과를 근거로 시험관내 실험이외의 추가적인 연구를 통해 폐기종과 폐고혈압 치료제로서 택사(澤瀉) 약침액(藥鍼液)의 활용을 고려해 볼 수 있을 것으로 기대한다.

Effects of Astragali Radix Extracts on the Elastase Activity and DPPH and NO Scavenging Activities (황기(黃芪)의 elastase 활성과 DPPH, NO 소거능에 미치는 영향)

  • Mou, Jong-Cheng;Lee, Se-Na;Kim, Myung-Gyou;Kim, Myoung-Hee;Kim, Hyung-Jun;Jo, Hak-Jun;Leem, Kang-Hyun
    • The Korea Journal of Herbology
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    • v.26 no.1
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    • pp.59-63
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    • 2011
  • Objectives : Elastic fibers are found in the skin, lungs, arteries, veins and other structures. The defects of elastic matrix aggravate hypertension which is associated with alteration in the great arteries, arteries, and arterioles. The elastase inhibitors were undergoing in clinical studies about emphysema and pulmonary hypertension. This study was designed to investigate the effect of Astragali Radix extracts (AR) on elastase activity and anti-oxidative effects. Methods : The elastase inhibitory activity and DPPH (1,1-diphenyl-2-picrylhydrazyl) and NO free radical scavenging activities of AR were measured. Results : The elastase activity was significantly inhibited by AR. The significant DPPH and NO free radical scavenging activities were observed in AR as well. Conclusion : AR showed the anti-elastase effects and anti-oxidative activities in vitro. These results suggest that AR may be a possible drug for the treatment of pulmonary emphysema and pulmonary hypertension.

Effects of $Angelica$ $Gigantis$ Pharmacopuncture Extracts on the Elastase Activity and DPPH and NO Scavenging Activities (당귀약침액의 Elastase 효소 활성과 DPPH, NO 소거능에 미치는 영향)

  • Lee, Se-Min;Lim, Seong-Chul;Lee, Yun-Kyu;Kim, Jae-Soo
    • Journal of Acupuncture Research
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    • v.28 no.6
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    • pp.35-42
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    • 2011
  • 목적 : Elastin 섬유는 피부, 폐, 동맥, 정맥 등 조직에서 발견된다. Elastin 섬유의 손실은 대동맥, 동맥, 세동맥의 탄력 등과 연관되어 폐기종, 혈액순환 부전 및 폐동맥 고혈압을 악화시킬 수 있다. 본 연구는 당귀약침액의 elastase 효소활성에 대한 효과와 항산화 효과에 대하여 연구하고자 계획되었다. 방법 : 당귀약침액의 elastase 억제효과와 DPPH, 및 NO 소거능을 측정하여 항산화 효과를 측정하였다. 결과 : 당귀약침액에서 통계적으로 유의한 elastase 활성 억제효과를 관찰할 수 있었다. 또한 통계적으로 강한 DPPH free radical 소거 효과가 관찰되었다. 그리고 NO 소거효과를 관찰할 수 있었으며, 그 효능은 pH 6.0에서 가장 강한 효능을 보였다. 결론 : 본 연구 결과에 의하면, 당귀약침액에는 유효한 elastase 활성 억제효과와 강한 항산화 효과가 있어, 폐기종 및 폐동맥 고혈압 치료에 유효한 작용이 있을 것으로 사료된다.

Measurement of Antioxidation Substances in Basil (바질의 항산화 물질 측정에 관한 연구)

  • Suh, Bong-Soon;Park, Myung-Hee
    • Journal of the East Asian Society of Dietary Life
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    • v.20 no.1
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    • pp.54-59
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    • 2010
  • Basil is known to contain six types of polyphenols that engage in physiological activation; protocateuic acid, caffeic acid, chlorogenic acid, courmaric acid, rosmarinic acid and quercetin. In this study, the antioxidants in eight types of basil were evaluated. Specifically, the antioxidative activation of basil was evaluated based on the relationship between active oxygen scavenging (DPPH radical-scavenging), which was used as an index showing the content and functionality of the polyphenol compounds in basil, and $Fe^{2+}$/ascorbate (FTC) and 2-thiobarbituric acid (TBA). The total polyphenol content of the different types basil occurred in the following order: Dark-opal> Lettuce> Bush> Greek> Lemon> Sweet> Geno> Holy. The highest content Dark-opal was 173.3 mg, which was about three times greater than the lowest content Holy, which was 49.85 mg. In addition, DPPH radical-scavenging by Dark-opal 51%, which was the highest scavenging activity occurred in the folling order: Dark-opal> Lettuce> Bush> Greek> Lemon> Sweet> Geno> Holy. The antioxidative activation values measured using the FTC and TBA were the same as the value obtained using the DPPH method. Finally, the level of antioxidative activation measured using FTC, TBA and DPPH methods showed that the higher the content of polyphenol substance was, the stronger the antioxidative activation became.

DPPH Free Radical Scavenging Effect of the Aerial Parts of Desmodium oldhami (큰도둑놈의갈고리 지상부의 DPPH Free Radical 소거효과)

  • Yang, Seo-Kwon;Park, Sae-Rom;Ahn, Dal-Rae;Yang, Jae-Heon;Cho, Chong-Hyeon;Hwang, Yong-Hun;Park, Jong-Han;Kim, Dae-Keun
    • Korean Journal of Pharmacognosy
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    • v.41 no.3
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    • pp.180-184
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    • 2010
  • The antioxidant activity of Desmodium oldhami (Leguminosae) was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl). The methanolic extract of D. oldhami showed the strong antioxidant activity. Five compounds, kaempferol-3-O-rhamnopyranoside (afzeline) (1), quercetin-3-O-[$\alpha$-L-rhamnopyranosyl($1{\rightarrow}6$)-$\beta$-Dglucopyranoside] (rutin) (2), kaempferol-3-O-glucopyranoside (astragalin) (3), genistein-7-O-$\beta$-D-glucopyranoside (genistin) (4), kaempferol-3-O-rutinoside (5) were isolated from the active ethyl acetate soluble fraction of D. oldhami through repeated silica gel and Sephadex LH-20 column chromatography. Among them, compound 2 showed the most significant antioxidative effect on DPPH free radical scavenging test. Compounds 1-5 are reported for the first time from this plant.

Inhibitory Effect on NO, Scavenging Effect on DPPH Radical in Whallak-tang (활락탕(活絡湯)의 NO 억제, DPPH 소거 및 Microarray상 유전자 발현에 미치는 영향)

  • Kim, Cheol-hong;Youn, Hyoun-min;Jang, Kyung-jeon;Song, Choon-ho;Ahn, Chang-bum
    • Journal of Acupuncture Research
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    • v.21 no.5
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    • pp.69-78
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    • 2004
  • Objective : This study was undertaken to determine the inhibitory effect of Whallak-tang against Nitric Oxide, the scavenging effect of Whallak-tang on DPPH radical. Methods : Both nitric concentration 1, 3, 6 and 24 hours after treatment with Vit. C and Whallak-tang at 0.1, 1, 10 mg/ml and scavenging effect 10, 20, 30 and 60 minute after treatment with Vit. C and Whallak-tang at 0.1, 1, 10 mg/ml were estimated. Results : The inhibitory effect of Whallak-tang against Nitric Oxide was dose-dependent. The scavenging effect of Whallak-tang on DPPH radical was dose-dependent. Conclusion : These results would provide important basic data on the possibility of the clinical treatment of Whallak-tang in musculoskeletal disease.

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DPPH Radical Scavenging Effect of the Aerial Parts of Fagopyrum esculentum and Isolation of Bioactive Flavonoids (메밀 지상부의 DPPH 라디칼 소거작용과 활성 플라보노이드의 분리)

  • Kim Sung-Ja;Kim Hyun-Joo;Park Jong-Cheol
    • Herbal Formula Science
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    • v.12 no.1
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    • pp.255-262
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    • 2004
  • The inhibitory effect of the aerial parts of Fagopyrum esculentum on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical was examined. The n-butanol fraction from the methanol extract of title plant showed stronger inhibitory effect than other fractions on DPPH radical. Two flavonoids were isolated from n-butanol fraction having the potent activity and elucidated as quercetin-3-O-${\alpha}$-L-rhamnoside and quercetin-3-O-rutinoside on the basis of spectral evidence. The $IC_{50}$ values of these compounds on DPPH radical were 6.56 ${\mu}M$ and 8.37 ${\mu}M$, respectively.

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Antioxidant Activity of Rosa rugosa (해당화의 항산화 효과)

  • 서영완;이희정;안종웅;이범종;문성기
    • KSBB Journal
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    • v.19 no.1
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    • pp.67-71
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    • 2004
  • An antioxidant activity of Rosa rugosa extract and its solvent-partitioned fractions was determined not only by measuring lipid peroxide produced when a mouse liver homogenate was exposed to the air at 37$^{\circ}C$, using thiobarbituric acid (TBA) but also by evaluating the free radical scavenging effect against DPPH radical, authentic peroxynitrite, and 3-morpholinsydnonimine (SIN-1). All its partitioned fractions including crude extract showed potent scavenging effect against DPPH radical, peroxynitrite, and lipid peroxidation. n-BuOH fraction, in particular, was found to be the most effective in DPPH radical scavenging ability as well as inhibition against lipid peroxidation. The 15% aqueous MeOH fraction also showed a strong potency which was slightly lower than n-BuOH fraction. Based on these results, we suggest that Rosa rugosa could be useful for preventing an oxidative damage.

Screening of Functional Compound of Abeliophyllum distichum Nakai for Optimize Extraction Condition and Efficacy Evaluation (미선나무의 추출조건 확립 및 효능평가를 통한 기능성 탐색)

  • Cho, Seok-Cheol;Kim, Myong-Ki
    • Journal of Convergence for Information Technology
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    • v.7 no.6
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    • pp.61-67
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    • 2017
  • To evaluate optimum conditions of extract active components, extraction of Abeliophyllum distichum Nakai was performed with various ethanol and water mixture at ethanol concentration of 0, 20, 40, 60, 80 and 100%(v/v) and extraction methods. Higher polyphenol and flavonoids contents of extract achieved at 2 hours of reflux extraction in 80% ethanol. The highest DPPH free radical scavenging activity of the stem extracts by soaking method and butanol fractions of leaf extracts are 71.3% and 103.3% respectively. The results suggested that Abeliophyllum distichum Nakai can be used for valuable natural resource.

Convergence study of oxidative stress from fraction of Xanthium strumarium L. (도꼬마리 추출물의 산화적 스트레스에 대한 융합연구)

  • Min, Young-Sil
    • Journal of the Korea Convergence Society
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    • v.8 no.12
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    • pp.215-220
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    • 2017
  • Xanthium strumarium L. is an annual plant belongs to the family Asteraceae which is is called a 'Cocklebur' that is used for medicinal purposes. Convergent phyto-activity of various extracts of Xanthium strumarium L. (Asteraceae) was examined. We estimated antioxidant activity from ground part and fruit extract of X. strumarium using 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) and ABTS assay. The extract of X. strumarium was separated each fraction that of ethanol, petroleum ether, and ethyl acetate. It showed potent radical scavenging effect against the DPPH radical and ABTS. The study revealed that X. strumarium could be used as a potential source of natural antioxidant.