• Title, Summary, Keyword: DPPH

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Effects of Meretrix Pharmacopuncture Extracts on the Elastase Activity and DPPH and NO Scavenging Activities (문합약침액(文蛤藥鍼液)의 Elastase 활성과 DPPH, NO 소거능에 미치는 영향)

  • Leem, Kang-Hyun
    • Journal of Acupuncture Research
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    • v.28 no.2
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    • pp.117-123
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    • 2011
  • 목적 : 엘라스타제는 폐의 탄성 섬유를 파괴하여 폐기종과 폐고혈압을 유발하는 역할을 한다. 이에 본 연구는 문합약침액(文蛤藥鍼液)의 엘라스타제 억제효능과 항산화효능을 측정하여 폐기종과 폐고혈압 치료의 가능성을 확인하고자 실시하였다. 방법 : 문합약침액(文蛤藥鍼液)의 엘라스타제에 대한 억제효능과 di(phenyl)-(2,4,6-trinitrophenyl) iminoazanium (DPPH) 및 nitric oxide(NO) 자유기 소거능을 측정하였다. 결과 : 문합약침액(文蛤藥鍼液)은 엘라스타제 활성을 통계적으로 유의하게 억제하였고, DPPH 및 NO 자유기 소거능이 있음을 통계적으로 유의하게 확인하였다. 결론 : 본 연구를 통해 문합약침액(文蛤藥鍼液)의 엘라스타제 억제효능과 항산화 효능이 확인되었다. 본 연구 결과를 근거로 시험관 내 실험 이외의 추가적인 연구를 통해 폐기종과 폐고혈압 치료제로서 문합약침액(文蛤藥鍼液)의 활용을 고려해 볼 수 있을 것으로 기대한다.

Radical Scavenging Effect of Methanol Extracts from Seaweeds and Their Active Compounds (해조류 추출물 및 활성성분의 라디칼 소거능)

  • So, Mi Jung;Cho, Eun Ju
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.3
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    • pp.187-191
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    • 2007
  • The radical scavenging activity of methanol extracts of seaweeds and their active compounds, alginic acid, fucoidan and phloroglucinol, were investigated under in vitro. Among methanol extracts of seaweeds (sea mustard, sea tangle, seaweed papulosa, fusiforme, sea lettuce, purple laver and chlorella), seaweed papulosa and sea tangle showed strong scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical (${\cdot}OH$). In addition, under in vitro, the scavenging activities on DPPH radical of alginic acid and fucoidan, which are active compounds of brown algae, and phloroglucinol, the active compound from Ecklonia species, were evaluated and compared. Fucoidan and phloroglucinol showed strong DPPH scavenging effect, in particular, phloroglucinol had strongest activity among the active compounds. On the other hand, alginic acid did not exert DPPH scavenging activity. From the present study, we could confirm the antioxidative activity of seaweeds and its active compounds.

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Varietal Variation in Antioxidative Activity of Rice Grain by DPPH and TBA Methods

  • Chung, Ill-Min;Kim, Kwang-Ho;Ahn, Joung-Kuk;Lee, Jin-Ohk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.4
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    • pp.261-266
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    • 2000
  • This study was to investigate antioxidative activity of rice grain using 1, 1-Diphenyl-2-picrylhydrazyl(DPPH) and thiobarbituric acid (TBA) method and germination ability for screening rice varieties with high antioxidative activities on korean native and foreign rice varieties harvested in 1998 and 1999. The average antioxidative activity of foreign rice varieties (DPPH 63.5% and TBA 55.2%) was significantly higher than that of native rice varieties (DPPH 47.2% and TBA 45.6%) on varieties harvested in 1999. The promptness index (PI) of native rice varieties was higher in stored rices for three months (mean PI=160.7) than that of stored rices for a year (mean PI=141.6). On the other hand, the PI of foreign rice varieties was higher in stored rices stored for a year (mean PI=176.7) than that of stored rices for three months (mean PI=157.5). Varieties with high redness of hulled rice (a-value) showed significant lipid peroxidation inhibitory activity to DPPH in a stored rices for a year (r=${0.5744}^**$) and stored rices for three months (r=${0.5630}^**$) . These results indicate that the pigments of hulled rice varieties may play important antioxidative roles and colored rice varieties with higher antioxidative potentials can be developed and also may provide information with rice breeder to breed rice variety with a high antioxidative activity for a rapid screening of a small amounts of a large number of samples using color value.

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오미자(Schizandra Chinesis Baillon)추출물의 분획별 항상화활성 효과

  • Kim, Ji-Eun;Hwang, Hyeon-Ik;Lee, In-Sun;Mun, Hye-Yeon
    • 한국생물공학회:학술대회논문집
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    • pp.317-320
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    • 2002
  • To search for antioxidant compounds from plant resources, ethanol extracts of 10 plants species were investigated using DPPH method and SOD activity by NBT method. The highest activity was shown in the ethanol extracts of Schizandra Chinesis Baillon. And than, antioxidant effects and total polyphenol concentration were investigated in solvent fraction of ethanol extracts from Schizandra Chinesis Baillon. The DPPH inhibitory activity and SOD activity of ethyl acetate fraction (DPPH 51.6%, SOD 66.2%) and butanol fraction(DPPH 60.7%, SOD 67.4%) showed strong biological activities.

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Development of Resources for Functional Food and Biological Activity of Lentinus edoes mycelium (표고버섯 균사체의 생리활성과 기능성식품 소재 개발)

  • Kim, Soo-Min;Kim, Eun-Ju
    • The Korea Journal of Herbology
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    • v.26 no.2
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    • pp.25-30
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    • 2011
  • Objectives : This study was carried out to investigate an antioxidant ability, the change of antioxidant ability, antimicrobial activity and anticancer in functional meat ball and sausage prepared from Lentinus edoes mycelium and texturized soy protein. Methods : This experiments was carried out to investigate antioxidant ability(TBARS, DPPH, SOD-like ability), antibacterial activity and anticancer ability using sarcoma 180 extracts from Lentinus edoes mycelium. Result : Anticancer ability of Lentinus edoes mycelium showed a 28% survival rate and 63% of inhibition rate of tumor, which showed $1.30{\pm}0.4g$ of tumor weight. These results revealed an effective Lentinus edoes mycelium resources as anticancer sources. After heating peoducts prepared from Lentinus edoes mycelium, these products doesn't showed difference between after heating and before heating in measuring of SOD-like activity and DPPH. In DPPH experiment, ethanol extracts showed a high DPPH value as 90.85%, but hot water extracts showed 82.14% in DPPH value. Conclusion : In conclusion, it is very useful resources for preparing functional food on the basis of results from antioxidant(TBARS, DPPH, SOD-like ability), antibacterial activity and anticancer ability using sarcoma 180.

Optimization for Pigment Production and Antioxidative Activity of the Products by Bacillus subtilis DC-2 (Bacillus subtilis DC-2의 색소 생성 및 그 생성물에 대한 항산화성의 최적화)

  • 정영건;최웅규;지원대;정현채;최동환
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.6
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    • pp.1039-1043
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    • 1997
  • Correlation among color intensity, electron donating ability to $\alpha$, $\alpha$-diphenyl-$\beta$-dicrylhydrazy(DPPH) and cultivation conditions by Bacillus subtilis DC-2 were tested with response surface methodology. Both of pigment generation ability and DPPH were more affected by temperature than any other factor. The highest correlation was appeared between color intensity and DPPH as 0.8364 which is significant at 1% level. After fixing cultivation time which is not significant at 10% level to 84hrs as optical cultivation time, response surface methodology was conducted in regarding temperature and color intensity. As a result of overlapped contour map of color intensity and DPPH, when cultivation temperature was in the range of 38.9~41.1$^{\circ}C$ and pH was in the range of 8.34~9.12, optical density of color intensity was predicted higher than 0.374 at 390nm and DPPH was infered higher than 1.310 at 528nm. In the range of optical culture condition, cultivation temperature, pH and cultivation time was fixed to 4$0^{\circ}C$, 8.5 and 85hrs, respectively. In resulting, observation value of color intensity and DPPH was in the range of anticipation value as 0.386 at 390nm and 1.332 at 528nm respectively.

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DPPH Free Radical Scavengers from the Aerial Part of Trigonotis peduncularis Bentham (꽃마리 지상부의 DPPH Free Radical 소거활성 성분)

  • Lee, Eun Byeol;Ahn, Dalrae;Lee, Tae Gwan;Eun, Jae Soon;Jeon, Hoon;Cha, Dong Seok;Cui, Xun;Shin, Tae-Yong;Cho, Chong Hyeon;Kim, Dae Keun
    • Korean Journal of Pharmacognosy
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    • v.44 no.3
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    • pp.230-234
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    • 2013
  • In the course of screening for antioxidant compounds by measuring the DPPH radical scavenging activity, a total extract of the aerial part of Trigonotis peduncularis (Boraginaceae) was found to show potent DPPH radical scavenging activity. Subsequent activity-guided fractionation of the methanolic extract of T. peduncularis led to the isolation of seven compounds, kaempferol (1), quercetin (2), trigonotin A (3), astragalin (4), trigonotin C (5), kaempferol-3-O-${\alpha}$-L-rhamnopyranosyl(1${\rightarrow}$6)-${\beta}$-D-glucopyranoside (6), and rutin (7) from the active ethyl acetate soluble fraction. Their structures were elucidated by spectroscopic studies. Among seven compounds, compounds 1, 2, 4, 6 and 7 were isolated for the first time from this plant, and compounds 2 and 7 showed the significant DPPH free radical scavenging activities.

Application and High Throughput Screening of DPPH Free Radical Scavenging Activity by Using 96-Well Plate (96-well plate를 이용한 DPPH free radical 소거활성 측정과 그 응용)

  • Choi, Jung-Sup;Oh, Jung-Im;Hwang, In-Taek;Kim, Sung-Eun;Chun, Jae-Chul;Lee, Byung-Hoi;Kim, Jin-Seok;Kim, Tae-Joon;Cho, Kwang-Yun
    • The Korean Journal of Pesticide Science
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    • v.7 no.2
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    • pp.92-99
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    • 2003
  • A 96-well plate was applied to determine the DPPH free radical scavenging activity using 107 plant-specific enzyme inhibitors and 100 unknown plant-originated extracts. The final optimum volume was $250{\mu}L$ containing $100{\mu}M$ DPPH ethanolic solution at pH 7.8. In this condition, the radical scavenging activities were significantly increased by two known antioxidants consisting of ascorbate and a-tocopherol in a concentration-dependent manner. Among the 107 inhibitors, ampicillin and gallic acid showed 90.2% and 92.6% antioxidant activity at $100{\mu}M$, respectively, and these results were consisted with previous findings. In the tested 100 natural materials at $50{\mu}g/mL$, antioxidant activity of AT-407 resulted in the highest of 90.1%, and 10 extracts including AT-388 and AT-443 showed over 70%. Our results suggest that the use of 96-well plate for determining DPPH free radical scavenging activity would be a suitable method to select antioxidant-like substances of both synthetic compounds and natural products.

Antioxidant Activity of Ogae Egg White Protein Hydrolysates using commercial Protease (단백질 분해효소를 이용한 오계란 가수분해물의 항산화 활성)

  • Ha, Yoo Jin;Ji, Joong Gu;Yoo, Sun Kyun
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.3
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    • pp.631-642
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    • 2017
  • Protein hydrolysates derived from plants and animals having antioxidant, suppression of hypertension, immunodulatory, alleviation of pain, and antimicrobial activity has been known as playing important role like hormone. This study was performed to hydrolysis of Ogae egg white protein using the six proteases. The antioxidant activity of the produced peptides was analyzed. As a result, the maximum value of hydrolysis was protamex(46.3%), DPPH radical scavenging was bromelain(57.23%), hydroxy radical scavenging was alcalase(30.21%), superoxide radical scavenging was alcalase(58.07%), and $Fe^{2+}$ chelation ability was alcalase(72.06%). Furthermore, the antioxidant Inhibition concentration ($IC_{50}$) of peptides was evaluated for each enzyme. As a result, the maximum value of alcalase was $Fe^{2+}$ cheating ability($IC_{50}$, 1.24 mg/mL), bromelain was DPPH radical scavenging($IC_{50}$, 2.46 mg/mL), flavourzyme was $Fe^{2+}$ cheating ability($IC_{50}$, 1.25 mg/mL), neutrase was DPPH radical scavenging($IC_{50}$, 3.64 mg/mL), papain was DPPH radical scavenging ($IC_{50}$, 3.82 mg/mL) and protamex was DPPH radical scavenging($IC_{50}$, 1.93 mg/mL). Therefore, we expect that peptides produced from Ogae egg white protein using protease enzyme are useful as an antioxidant functional food ingredients.

Study on Antioxidant Potency of Cuscutae Semen, Psoraleae Fructus, Cnidii Fructus and Epimedii Herba by DPPH Method (DPPH 방법을 통한 토사자, 보골지(補骨脂), 사상자(蛇床子), 음양곽(淫羊藿)의 항산화 활성에 대한 연구)

  • Oh, Myung-Sook;Kim, Do-Rim;Kang, Ji-Ung;Kim, San-Woong;Yu, Tae-Weon;Park, Jung-Yeul;Kim, Dong-Min;Park, Wan-Su;Chang, Mun-Seog;Park, Soo-Yeon;Park, Seong-Kyu
    • Herbal Formula Science
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    • v.13 no.2
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    • pp.101-110
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    • 2005
  • The present study was conducted to compare antioxidant activity of Cuscutae Semen, Psoraleae Fructus, Cnidii Fructus and Epimedii Herba by DPPH radical scavenging activity. The extract was studied using diphenyl-picryl-hydrazyl (DPPH) for DPPH method. DPPH radical scavenging activity was measured after. .10, 20 and 30 minutes. The extract was tested by 1, 5, 10, 50, 100, 500 and $1000{\mu}g/ml$ concentrations. The results showed that the extract scavenged DPPH radical with time-dependent manner. Also, the extract showed dose-dependent DPPH radical scavenging activity. The extract of Cuscutae Semen, Psoraleae Fructus, Cnidii Fructus and Epimedii Herba scavenged DPPH radical with the IC50 being 2.7, 3.2, 2.9 and 1.1 mg/ml, respectively. In conclusion, the extract of Epimedii Herba, Cuscutae Semen, Cnidii Fructus and Psoraleae Fructus have antioxidant activity for the treatment of male sterility.

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