• Title, Summary, Keyword: DEAD-box

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Identification and characteristics of DDX3 gene in the earthworm, Perionyx excavatus (팔딱이 지렁이(Perionyx excavatus) DDX3 유전자의 동정 및 특성)

  • Park, Sang Gil;Bae, Yoon-Hwan;Park, Soon Cheol
    • Journal of the Korea Organic Resources Recycling Association
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    • v.23 no.1
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    • pp.70-81
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    • 2015
  • Helicases are known to be a proteins that use the chemical energy of NTP binding and hydrolyze to separate the complementary strands of double-stranded nucleic acids to single-stranded nucleic acids. They participate in various cellular metabolism in many organisms. DEAD-box proteins are ATP-dependent RNA helicase that participate in all biochemical steps involving RNA. DEAD-box3 (DDX3) gene is belonging to the DEAD-box family and plays an important role in germ cell development in many organisms including not only vertebrate, but also invertebrate during asexual and sexual reproduction and participates in stem cell differentiation during regeneration. In this study, in order to identify and characterize DDX3 gene in the earthworm, Perionyx excavatus having a powerful regeneration capacity, total RNA was isolated from adult head containing clitellum. Full length of DDX3 gene from P. excavatus, Pe-DDX3, was identified by RT-PCR using the total RNA from head as a template. Pe-DDX3 encoded a putative protein of 607 amino acids and it also has the nine conserved motifs of DEAD-box family, which is characteristic of DEAD-box protein family. It was confirmed that Pe-DDX3 has the nine conserved motifs by the comparison of entire amino acids sequence of Pe-DDX3 with other species of different taxa. Phylogenetic analysis revealed that Pe-DDX3 belongs to a DDX3 (PL10) subgroup of DEAD-box protein family. And it displayed a high homology with PL10a, b from P. dumerilii.

DEAD/DExH-Box RNA Helicases in Selected Human Parasites

  • Marchat, Laurence A.;Arzola-Rodriguez, Silvia I.;Cruz, Olga Hernandez-de la;Lopez-Rosas, Itzel;Lopez-Camarillo, Cesar
    • The Korean Journal of Parasitology
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    • v.53 no.5
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    • pp.583-595
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    • 2015
  • DEAD/DExH-box RNA helicases catalyze the folding and remodeling of RNA molecules in prokaryotic and eukaryotic cells, as well as in many viruses. They are characterized by the presence of the helicase domain with conserved motifs that are essential for ATP binding and hydrolysis, RNA interaction, and unwinding activities. Large families of DEAD/DExH-box proteins have been described in different organisms, and their role in all molecular processes involving RNA, from transcriptional regulation to mRNA decay, have been described. This review aims to summarize the current knowledge about DEAD/DExH-box proteins in selected protozoan and nematode parasites of medical importance worldwide, such as Plasmodium falciparum, Leishmania spp., Trypanosoma spp., Giardia lamblia, Entamoeba histolytica, and Brugia malayi. We discuss the functional characterization of several proteins in an attempt to understand better the molecular mechanisms involving RNA in these pathogens. The current data also highlight that DEAD/DExH-box RNA helicases might represent feasible drug targets due to their vital role in parasite growth and development.

Cold Shock Response and Low Temperature Stable Transcript of DEAD-box RNA Helicase in Bacillus subtilis (DEAD-box RNA Helicase 유전자가 결핍된 Bacillus subtilis의 저온 충격 반응성과 저온 안정성 전사물)

  • Oh, Eun-Ha;Lee, Sang-Soo
    • Korean Journal of Microbiology
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    • v.47 no.4
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    • pp.289-294
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    • 2011
  • We investigated the cold shock sensitivity of DEAD-box RNA helicase gene deleted strains of in Bacillus subtilis CU1065. To understand cold shock effects, cells were cultivated at $37^{\circ}C$ to log phase ($O.D_{600}$=0.5-0.6) and then temperature was shifted to $15^{\circ}C$. Cold shock slow down the growth rate of wild type and deleted strains of DEAD-box RNA helicase gene (ydbR, yfmL, yqfR, deaD). The growth rate of ydbR deleted strain is 5 times severely reduced compared to that of wild type strain (CU1065). But the growth rate of other three (yfmL, yqfR, deaD) deleted strains is nearly equal to the growth rate of wild type. Compared to $37^{\circ}C$, the amount of ydbR and yqfR mRNA transcripts are increased at the growth temperature of $15^{\circ}C$. On the other hands the mRNA transcripts of yfmL and deaD are not changed at both conditions of $37^{\circ}C$ and $15^{\circ}C$. Upon cold shock treatment ydbR mRNA transcript is clearly increased. After treatment of rifampicin (bacteria transcription inhibitor) the amount of ydbR mRNA was measured. Temperature shift from $37^{\circ}C$ to $15^{\circ}C$ and rifampicin treatment showed slowly decay of ydbR mRNA. But at $37^{\circ}C$ and rifampicin treatment ydbR mRNA is rapidly reduced. These results showed that cold shock induction of ydbR mRNA resulted from the stability of ydbR mRNA and not from the transcription induction of ydbR. In relation to these results, we found the cold box element of csp (cold shock protein gene) in 5' untranslated region of ydbR gene. Cold shock induction of ydbR is caused by the stability of ydbR mRNA like the stability of csp mRNA.

Structural Design of Box Beam Header

  • Jang, Sang-Sik;Park, Young-Ran;Kim, Yun-Hui
    • Journal of the Korea Furniture Society
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    • v.18 no.4
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    • pp.287-295
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    • 2007
  • To obtain a design data for box beams used as headers in light-frame timber construction, $2{\times}6\;(38{\times}140mm),\;2{\times}8\;(38{\times}184mm),\;2{\times}10\;(38{\times}235mm)\;and\;2{\times}12\;(38{\times}286mm)$ members were built as box beam specimens for bending tests. The allowable bending stresses for box beams were obtained through bending tests of these specimens, and span tables were calculated for various loading conditions based on the allowable bending stresses obtained. The allowable bending stresses were determined as the bending stresses at 10mm deflection of specimens from the results of bending tests of box beam specimens. Span tables for box beams were obtained assuming five loading conditions for headers used in exterior walls and two loading conditions for headers used in interior walls. Among these 7 loading conditions, 5 loading conditions applied to headers in exterior walls included the dead loads, the live loads and the snow loads and 2 loading conditions applied to headers in interior walls included the dead loads and the live loads.

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Sex Determination of Cattle Meat by Polymerase Chain Reaction Amplification of the DEAD Box Protein (DDX3X/DDX3Y) Gene

  • Gokulakrishnan, P.;Kumar, R.R.;Sharma, B.D.;Mendiratta, S.K.;Sharma, D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.5
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    • pp.733-737
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    • 2012
  • Determination of sex origin of cattle meat by fast and reliable molecular methods is an important measure to ensure correct allocation of export refunds particularly in European countries and also female cattle (cow) slaughter is legally banned in India because of religious beliefs. Based on the DEAD box protein gene located on the X and Y chromosomes, 2 pair of primers were designed and the system of PCR was optimized. Upon PCR amplification, male tissue showed 2 bands, while female tissue resulted in only one band. The accuracy and specificity of the primers was assessed using DNA template extracted from cattle meat of known sex. The protocol was subjected to a blind test and showed 100% concordance, proving its accuracy and reliability.

Mirror vision for car Black box (자동차 블랙박스를 위한 미러 비전)

  • Kim, Eun-Ho;Lim, Myoung-Sub
    • Proceedings of the KIEE Conference
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    • pp.369-372
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    • 2007
  • about commercial business of car black box, mirror vision for car black box deal with analysis of Existing Car Black boxes in market to provide the objective information associated with surrounding scene of car instead of witness, we experimented on suitable structure of all direction to cover surrounding of car considering dead zone where can't see at short distance and realized simple structure of gathering scene using mirror and lens and by saving the number of camera and MUX of pre-circut

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Sensitivity Analysis by Parametric Study of Load Factor for a Concrete Box Girder Railway Bridge Using Limit State Design

  • Yeo, Inho;Sim, Hyung-Bo;Kim, Daehwan;Kim, Yonghan
    • International Journal of Railway
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    • v.8 no.1
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    • pp.5-9
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    • 2015
  • Reliability based limit state design method is replacing traditional deterministic designs such as allowable stress design and/or ultimate strength design methods in world trends. European design code(Eurocode) has adopted limit state design, and Korea road bridge design standard has also recently been transferred to limit state design method. In this trend, Korea railroad design standard is also preparing for adopting the same design concept. While safety factors are determined empirically in traditional design, load combinations as well as load factors are determined by solving limit state equations. General partial safety factors are evaluated by using AFORM(Advanced First Order Reliability Method) in the reliability based limit state design method. In this study sensitivity analysis is carried out for a dead load factor and a live load factor. Relative precisions of the dead load and the live load factors are discussed prior to the AFORM analysis. Furthermore the sectional forces of design and the material quantities required by two different design methods are compared for a PSC box girder railway bridge.

Equivalent Transverse Forces due to Longitudinal Prestressing of Box Girders (박스 거더의 종방향 프리스트레싱에 의한 횡방향 등가하중)

  • 양인환
    • Proceedings of the Korea Concrete Institute Conference
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    • pp.955-960
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    • 2003
  • For box girders in which the longitudinal tendon is profiled in the inclined webs. longitudinal prestressing force will induce transverse effects as well as longitudinal ones. In this paper. the method estimating transverse effects induced by longitudinal prestressing is proposed. The transverse effects in the slabs of box girders due to longitudinal prestressing are investigated. Numerical analyses are carried out depending on the parameters such as web inclination and ratio of girder length to tendon eccentricity. Analysis results show that when only prestressing are considered the magnitude of stresses in the slabs of box grder is not so large. However. if the other stresses due to dead and live load et al. are superposed on these stresses. it may be that the longitudinal prestressing effects are significant.

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