• Title, Summary, Keyword: DE

Search Result 8,432, Processing Time 0.122 seconds

Promoter Polymorphisms of ST3GAL4 and ST6GAL1 Genes and Associations with Risk of Premalignant and Malignant Lesions of the Cervix

  • de los Angeles Rivera-Juarez, Maria;Rosas-Murrieta, Nora Hilda;Mendieta-Carmona, Victoriano;Hernandez-Pacheco, Raquel Esneidy;Zamora-Ginez, Irma;Rodea-Avila, Carlos;Apresa-Garcia, Teresa;Garay-Villar, Onix;Aguilar-Lemarroy, Adriana;Jave-Suarez, Luis Felipe;Diaz-Orea, Maria Alicia;Milflores-Flores, Lorena;Reyes-Salinas, Juan Salvador;Ceja-Utrera, Francisco Javier;Vazquez-Zamora, Victor Javier;Vargas-Maldonado, Tomas;Reyes-Carmona, Sandra;Sosa-Jurado, Francisca;Santos-Lopez, Gerardo;Reyes-Leyva, Julio;Vallejo-Ruiz, Veronica
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.15 no.3
    • /
    • pp.1181-1186
    • /
    • 2014
  • Sialyltransferase gene expression is altered in several cancers, including examples in the cervix. Transcriptional regulation of the responsible genes depends on different promoters. We aimed to determine the association of single-nucleotide polymorphisms in the B3 promoter of the ST3GAL4 gene and the P1 promoter of the ST6GAL1 gene with cervical premalignant lesions or cervical cancer. A blood sample and/or cervical scrapes were obtained from 104 women with normal cytology, 154 with premalignant lesions and 100 with cervical cancer. We also included 119 blood samples of random donors. The polymorphisms were identified by sequencing from PCR products. For the B3 promoter, a fragment of 506 bp (from nucleotide -408 to +98) was analyzed, and for the P1 promoter a 490 bp (-326 to +164) fragment. The polymorphism analysis showed that at SNP rs10893506, genotypes CC and CT of the ST3GAL4 B3 promoter were associated with the presence of premalignant lesions (OR=2.89; 95%CI 1.72-4.85) and cervical cancer (OR=2.23; 95%CI 1.27-3.91). We detected only one allele of each polymorphism in the ST6GAL1 P1 promoter. We did not detect any genetic variability in the P1 promoter region in our study population. Our results suggest that the rs10893506 polymorphism -22C/T may increase susceptibility to premalignant and malignant lesions of the cervix.

Behaviour of axially loaded RC columns strengthened by steel angles and strips

  • Adam, J.M.;Ivorra, S.;Gimenez, E.;Moragues, J.J.;Miguel, P.;Miragall, C.;Calderon, P.A.
    • Steel and Composite Structures
    • /
    • v.7 no.5
    • /
    • pp.405-419
    • /
    • 2007
  • This paper presents the development of some numerical models based on the results of laboratory tests performed on axially loaded RC columns strengthened with steel angles and strips. These numerical models consider the nonlinearity of the building materials and the effects of the contact interfaces between different materials. The results of the finite element models accurately describe the general behaviour of the strengthened columns. This study allows engineers to assess the relative importance of the mechanisms acting on the strengthened RC columns. Constructive recommendations are also provided in this paper.

Presence of Proboscipedia and Caudal Gene Homologues in a Bivalve Mollusc

  • Carpintero, Pablo;Pazos, Antonio Juan;Abad, Marcelina;Sanchez, Jose Luis;De La Luz Perez-Paralle, Maria
    • BMB Reports
    • /
    • v.37 no.5
    • /
    • pp.625-628
    • /
    • 2004
  • Homeobox genes encode a family of transcription factors that have essential roles in regulating the development of eukaryotes. Although they have been extensively studied in different phyla, relatively little is known about homeobox-containing genes and their function in molluscs. In this study, we used a polymerase chain reaction to investigate homeobox genes in the bivalve mollusc Pecten maximus. Four different homeobox sequences were identified; two were homologues of the non-Hox cluster gene caudal and the two remaining sequences had a significant homology to the ANT-C gene proboscipedia. These sequences represent the first cad and pb homologues isolated from a member of the class Bivalvia, phylum Mollusca.

Purification, and Biochemical and Biophysical Characterization of Cellobiohydrolase I from Trichoderma harzianum IOC 3844

  • Colussi, Francieli;Serpa, Viviane;Da Silva Delabona, Priscila;Manzine, Livia Regina;Voltatodio, Maria Luiza;Alves, Renata;Mello, Bruno Luan;Nei, Pereira Jr.;Farinas, Cristiane Sanches;Golubev, Alexander M.;Santos, Maria Auxiliadora Morim;Polikarpov, Igor
    • Journal of Microbiology and Biotechnology
    • /
    • v.21 no.8
    • /
    • pp.808-817
    • /
    • 2011
  • Because of its elevated cellulolytic activity, the filamentous fungus Trichoderma harzianum has a considerable potential in biomass hydrolysis applications. Trichoderma harzianum cellobiohydrolase I (ThCBHI), an exoglucanase, is an important enzyme in the process of cellulose degradation. Here, we report an easy single-step ion-exchange chromatographic method for purification of ThCBHI and its initial biophysical and biochemical characterization. The ThCBHI produced by induction with microcrystalline cellulose under submerged fermentation was purified on DEAE-Sephadex A-50 media and its identity was confirmed by mass spectrometry. The ThCBHI biochemical characterization showed that the protein has a molecular mass of 66 kDa and pI of 5.23. As confirmed by smallangle X-ray scattering (SAXS), both full-length ThCBHI and its catalytic core domain (CCD) obtained by digestion with papain are monomeric in solution. Secondary structure analysis of ThCBHI by circular dichroism revealed ${\alpha}$- helices and ${\beta}$-strands contents in the 28% and 38% range, respectively. The intrinsic fluorescence emission maximum of 337 nm was accounted for as different degrees of exposure of ThCBHI tryptophan residues to water. Moreover, ThCBHI displayed maximum activity at pH 5.0 and temperature of $50^{\circ}C$ with specific activities against Avicel and p-nitrophenyl-${\beta}$-D-cellobioside of 1.25 U/mg and 1.53 U/mg, respectively.

Simulation of Electric Vehicles Combining Structural and Functional Approaches

  • Silva, L.I.;Magallan, G.A.;De La Barrera, P.M.;De Angelo, C.H.;Garcia, G.O.
    • Journal of Electrical Engineering and Technology
    • /
    • v.9 no.3
    • /
    • pp.848-858
    • /
    • 2014
  • In this paper the construction of a model that represents the behavior of an Electric Vehicle is described. Both the mechanical and the electric traction systems are represented using Multi-Bond Graph structural approach suited to model large scale physical systems. Then the model of the controllers, represented with a functional approach, is included giving rise to an integrated model which exploits the advantages of both approaches. Simulation and experimental results are aimed to illustrate the electromechanical interaction and to validate the proposal.

Self-sustained n-Type Memory Transistor Devices Based on Natural Cellulose Paper Fibers

  • Martins, Rodrigo;Pereira, Luis;Barquinha, Pedro;Correia, Nuno;Goncalves, Goncalo;Ferreira, Isabel;Dias, Carlos;Correia, N.;Dionisio, M.;Silva, M.;Fortunato, Elvira
    • Journal of Information Display
    • /
    • v.10 no.4
    • /
    • pp.149-157
    • /
    • 2009
  • Reported herein is the architecture for a nonvolatile n-type memory paper field-effect transistor. The device was built via the hybrid integration of natural cellulose fibers (pine and eucalyptus fibers embedded in resin with ionic additives), which act simultaneously as substrate and gate dielectric, using passive and active semiconductors, respectively, as well as amorphous indium zinc and gallium indium zinc oxides for the gate electrode and channel layer, respectively. This was complemented by the use of continuous patterned metal layers as source/drain electrodes.