• Title, Summary, Keyword: Cumulative Power of Discrimination

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Estimation of Genetic Characteristic and Cumulative Power of Breed Discrimination Using Microsatellite Markers in Hanwoo (Microsatellite Marker를 사용한 한우 품종 식별력 및 유전적 특성 분석)

  • Oh, Jae-Don;Lee, Jin-Ah;Kong, Hong-Sik;Park, Keong-Do;Yoon, Du-Hak;Jeon, Gwang-Ju;Lee, Hak-Kyo
    • Journal of Embryo Transfer
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    • v.23 no.3
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    • pp.203-209
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    • 2008
  • To estimate the genetic characteristics and cumulative power of discrimination (CPD) existing among Hanwoo (Korean cattle) and exotic foreign population (Angus, Herford, Charolais, Holstein) we used a total of 414 genomic DNAs from five breeds population (Hanwoo, Angus, Hereford, Charolais, Holstein). Genetic characteristics indices including mean allele number among loci, unbiased heterozygosity ($h_i$) within locus and polymorphic information content (PIC) and unbiased average heterozygosity (H) among loci in four breeds were calculated using the generated allele frequencies by each marker. The mean allele numbers for all loci ranged between 5 and 7 while heterozygosity (H) ranged from 0.75 (HW) to 0.64 (HF) among loci and across breeds heterozygosity (H) was 0.69. The generated unbiased average heterozygosity among loci in each breed was integrated to the global formula of CPD resulting in 99.71 % within the populations. The genetic variation of HW (Hanwoo) showed highest estimates among the analyzed breeds.

Estimation of the Cumulative Power of Discrimination in Haimen Chicken Populations with Ten Microsatellite Markers

  • Olowofeso, O.;Wang, J.Y.;Shen, J.C.;Chen, K.W.;Sheng, H.W.;Zhang, P.;Wu, R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.8
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    • pp.1066-1070
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    • 2005
  • To estimate the cumulative power of discrimination (CPD) existing within Haimen chicken populations in China, we isolated a total of 252 genomic DNAs from four chicken populations (Rugao, Jiangchun, Wan-Nan and Cshiqishi) through a saturated salt procedure. All the genomic DNAs were used in a polymerase chain reaction (PCR) with ten microsatellite markers. Amplified PCR-products with the selected markers were separated on a 12% polyacrylamide gel with pBR322DNA/MspI used as internal standard marker. Genetic diversity indices including mean allele number among loci, unbiased heterozygosity ($h_i$) within locus, effective number of alleles ($N_e$) and polymorphism information content (PIC) as well as the unbiased average heterozygosity (H) among loci in the populations were calculated using the generated allele frequencies by each marker. The mean allele number for all loci ranged between 4.00${\pm}$0.33 (Rugao) to 4.90${\pm}$0.48 (Cshiqishi) and across populations for all loci was 4.60${\pm}$0.20, while (H) ranged from 0.65${\pm}$0.03 (Rugao) to 0.69${\pm}$0.03 (Jiangchun) among loci and across populations, (H) was 0.67${\pm}$0.01. The generated unbiased average heterozygosity among loci in each population was integrated to the global formula of CPD and the result demonstrated that the CPD within the four Haimen chicken populations was 98.75%.

Estimation of Genetic Characteristics and Cumulative Power of Discrimination in Korean Native Chicken and Korean Native Commercial Chicken (토종닭 순계와 실용계의 유전적 특성 및 품종식별력 분석)

  • Oh, Jae-Don;Lee, Kun-Woo;Seo, Ok-Suk;Cho, Byung-Wook;Jeon, Gwang-Joo;Lee, Hak-Kyo;Kong, Hong-Sik
    • Journal of Life Science
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    • v.20 no.7
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    • pp.1086-1092
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    • 2010
  • To estimate the genetic characteristics and cumulative power of discrimination (CPD) within Korean native commercial chicken, we used a total of 395 genomic DNAs from six breeds population (Korean Native Red chicken: R, Korean Native Yellow chicken: Y, Korean native Commercial Chicken: C, Ogal chicken: S, Hy-Line Brown: H, White Leghorn: W). Genetic diversity indices including mean allele number among loci, unbiased heterozygosity ($h_i$) within locus, effective number of alleles ($N_e$) and polymorphism information content (PIC) as well as the unbiased average heterozygosity (H) among loci in the populations were calculated using the generated allele frequencies by each marker. Frequencies of microsatellites markers were used to estimate heterozygosities and genetic distances. The nearest distance (0.119) was observed between the C and Y strains. The generated unbiased average heterozygosity among loci in each population was integrated to the global formula of CPD and the result demonstrated that the CPD within the six chicken populations was 99.461%.

Estimation of Genetic Characteristic and Cumulative Power of Discrimination using the Microsatellite Markers in Korean Native Chicken (Microsatellite Marker를 사용한 재래 닭 품종 유전적 특성 및 개체 식별력 분석)

  • Lee, Kun-Woo;Oh, Jae-Don;Lee, Jin-Ah;Cho, Kyu-Ho;Nam, In-Sik;Lee, Jun-Heon;Seo, Ok-Suk;Jeon, Gwang-Joo;Lee, Hak-Kyo;Kong, Hong-Sik
    • Korean Journal of Poultry Science
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    • v.37 no.1
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    • pp.81-87
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    • 2010
  • To estimate the genetic characteristics and cumulative power of discrimination (CPD) Korean Native Chicken. We used a total of 195 genomic DNAs from four breeds population (Korean Native Red chicken: R, Korean Native Yellow chicken: Y, Korean Native Black chicken: L, Ogal chicken: S). Frequencies of microsatellites markers were used to estimate heterozygosities and genetic distances. The lowest distance (0.05) was observed between the R and L strains and the highest distance (0.158) between the L and S strains. Korean native chicken strains (R, Y, K) have each other comparatively near genetic distance. Cumulative power of discriminate (CPD) was 99.999% by including the 10 microsatellites loci individual identification system. And then matching probability in that two different individuals incidentally have same genotype was estimated to $0.36{\times}10^{-7}$. The system employing the 10 markers therefore provided to be applicable to individual identification in Korea native chicken.

Lower Body Somatotype Classification and Discrimination of Elderly Women According to Index (지수치를 이용한 노년 여성의 하반신 체형 유형화에 관한 연구)

  • 김수아;이경미;최혜선
    • Journal of the Korean Society of Costume
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    • v.53 no.6
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    • pp.117-130
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    • 2003
  • The purpose of this study is to provide the basic data on the development of ready-to-wear clothing for the elderly women as the population of the elderly has been constantly increasing as well as the purchasing power of the aged. The body measurements of 318 elderly women were taken. whose ages were over 60 years and enrolled in colleges for the elderly. sports centers. or business sites in Seoul and the neighboring districts. A total of 39 features in the lower body were used for the anthropometric measurement and analysis. The results of the study are as follows: 1. Indices of height and weight were used for factor analysis. cluster analysis, and discriminant analysis in order to 'classify lower body somatotype according to shape, excluding size factors. From the results of the factor analysis. the 5 factors showed the cumulative sum of square at 75.63%. 2. Somatotype were classified into two types according to a cluster analysis using height and weight dices. Type 1 is the group is relatively tall and has somewhat fat lower limbs. Type 2 is considered fat and has obesity factors around waist and abdomen area. The hit rate for the classified two groups showed the result at 95.9%.

Establishment of an Individual Identification System Based on Microsatellite Polymorphisms in Korean Cattle (Hanwoo)

  • Yoon, Du-Hak;Kong, Hong-Sik;Oh, Jae-Don;Lee, Jun-Heon;Cho, Byung-Wook;Kim, Jong-Dae;Jeon, Ki-Jun;Jo, Chang-Yun;Jeon, Gwang-Joo;Lee, Hak-Kyo
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.6
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    • pp.762-766
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    • 2005
  • This study was conducted to establish an individual identification system comprising of 19 microsatellite markers located on different bovine autosomes. The markers were typed on 257 animals from five cattle breeds. In total, 112 alleles were detected from the genotyping of 19 microsatellite markers. The average heterozygosities ranged from 0.292 to 0.824 and the polymorphic information content (PIC) ranged from 0.274 to 0.817 in Hanwoo. We found that there were differences in allele frequencies in Hanwoo when compared with other cattle breeds. The calculated cumulative power of discrimination (CPD) was 99.999% when nine microsatellite loci were used for analysis in the individual identification system. Also the matching probability, the probability that two unrelated animals would show the same genotypes, was estimated to be $0.44{\times}10^{-9}$. Therefore, the nine markers used in this study will be used for individual identification in two million Hanwoo individuals.

Establishment of Genetic Characteristics and Individual Identification System Using Microsatellite Loci in Jeju Native Horse (초위성체 DNA를 이용한 제주마 집단의 품종특성 및 개체식별 체계설정)

  • Cho, Byung-Wook;Jung, Ji-Hye;Kim, Sang-Wook;Kim, Heui-Soo;Lee, Hak-Kyo;Cho, Gil-Jae;Song, Ki-Duk
    • Journal of Life Science
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    • v.17 no.10
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    • pp.1441-1446
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    • 2007
  • This study was conducted to establish the individual identification system and to estimate the genetic characteristic of Jeju native horse (JNH) using 13 microsatellite markers located on different horse autosomes. The markers were genotyped on 191 animals from five horse breeds including Jeju native horse (JNH). In total, 138 alleles were detected from the genotypes of 13 microsatellite markers. The average heterozygosities ranged from 0.317 to 0.902 and the polymorphic information content (PIC) ranged from 0.498 to 0.799 in JNH. We found that there were significant differences in allele frequencies in JNH when compared with other horse breeds. In ATH4 marker, there were specific allele frequence pattern that some of allele only found in JNH, Mongolian horse (MONG) and Jeju racing horse (JRH). The calculated cumulative power of discrimination (CPD) was 99.9% when nine microsatellite loci were used for analysis in the individual identification system. Also, the matching probability that two unrelated animals would show the same genotypes, was estimated to be $0.60{\times}10^{-10}$. Therefore, in the nine markers used in this study can be used for individual identification in the Jeju native horse population.

Characterization of a Korean Traditional Porcine Breed Using Microsatellite Markers and the Establishment of an Individual Identification System (Microsatellite Marker를 이용한 한국재래돼지 집단의 품종특성 및 원산지 추적을 위한 개체식별체계 설정)

  • Kim, M.J.;Li, G.H.;Oh, J.D.;Cho, K.H.;Jeon, G.J.;Choi, B.H.;Lee, J.H.;Hong, Y.S.;Kong, H.S.;Lee, H.K.
    • Food Science of Animal Resources
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    • v.27 no.2
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    • pp.150-156
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    • 2007
  • This study was conducted to analyze the genetic characteristics of Korean Native Pigs(KNP), and to establish an individual identification system comprising many microsatellite markers located on different pig autosomes. Genotype data from 13 microsatellites typed in 446 animals was used to determine the validation of a method of individual identification in 4 KNP. A total of 112 alleles of the 13 microsatellites were detected and average heterozygosities(polymorphic information content) ranged from 0.286(0.423) to 0.686(0.796) in this study. Comparing the pattern of allele frequency among the KNP, Yorkshire, Landrace and Duroc breeds, there was specific differentiation between populations at multi-allelic loci. The cumulative power of discrimination(CPD) was 99.999% by including 10 microsatellite loci for the individual identification system. The probability that two different individuals incidentally have same genotype was estimated to be $0.36{\times}10^{-9}$. The system employing these 10 markers therefore proved to be applicable to the individual identification of KNP.