• Title, Summary, Keyword: Cortisol

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An Exploratory Study on Occupational Stress and Anxiety Through Salivary Cortisol and Self-Report Scale in Korean Nurses on Shift and Regular Work (근무형태별 간호사의 스트레스와 불안 조사: 타액 코티솔과 자가보고 척도를 이용한 탐색적 연구)

  • Suh, Minhee
    • Journal of Korean Biological Nursing Science
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    • v.19 no.3
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    • pp.206-213
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    • 2017
  • Purpose: The purpose of the study was to compare salivary cortisol levels, self-reported occupational stress, and anxiety before duty to those after duty in the shift and regular work nurses, and the relationships among them. Methods: Thirty nurses working in a tertiary hospital participated in the study. Salivary specimens were collected before and after daytime duty for 2 days. The occupational stress and state anxiety were also evaluated using self-report questionnaires. Results: The average level of salivary cortisol was $0.40{\mu}g/dL$ before duty and $0.20{\mu}g/dL$ after duty in Korean nurses. Overall levels of salivary cortisol, self-reported occupational stress, and anxiety were higher in shift working nurses than regular working nurses. In shift working nurses, the salivary cortisol gap between before and after duty was attenuated on the first day of daytime duty. Significant positive correlation was found between the level of cortisol before duty and anxiety after duty. Conclusion: Strategies to alleviate occupational stress and anxiety are needed for shift working nurses, especially for those with elevated levels of cortisol. It seems to require 3days to recover from attenuated cortisol rhythm during nighttime duty in shift working nurses.

Simultaneous quantitative analysis of salivary cortisol and cortisone in Korean adults using LC-MS/MS

  • Lee, Sang-Hoo;Kwon, Soon-Ho;Shin, Hye-Jin;Lim, Hwan-Sub;Singh, Ravinder J.;Lee, Kyoung-Ryul;Kim, Young-Jin
    • BMB Reports
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    • v.43 no.7
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    • pp.506-511
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    • 2010
  • The levels of salivary cortisol and cortisone in Korean adults were measured for the first time using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The salivary cortisol and cortisone were separated within 10 min. The regression coefficients (r) of the calibration curves were greater than 0.999 for the two steroids. The limits of quantitation (LOQ) were 0.2 ng/ml for cortisol and 1 ng/ml for cortisone. The intra-day precisions of the assay were <3.9% and 8.6% for cortisol and cortisone respectively, and the inter-day precisions were <1.9% and 4.3% for cortisol and cortisone, respectively. The salivary cortisone concentrations were approximately 4-9 times higher than those of salivary cortisol during the daytime. Diurnal rhythms, during which the cortisol and cortisone concentrations were higher in the morning than in the afternoon, were also observed. The present assay may be useful for the diagnosis of several adrenal dysfunctions in clinical biochemistry.

Effects of Cortisol on the Steroidogenesis and the Apoptosis of Human Granulosa-Lutein Cells (Cortisol이 사람 과립-황체화 세포의 스테로이드 생성과 세포자연사에 미치는 영향)

  • Kim, Jin-Hee;Yang, Hyun-Won
    • Development and Reproduction
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    • v.13 no.4
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    • pp.329-339
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    • 2009
  • Cortisol is present in high concentration in the ovary and its receptor is expressed in the ovarian cells. Moreover, cortisol is known to have a role in steroid synthesis and cell metabolism in human granulosa and lutein cells. However, little is known of the role of cortisol presenting in high concentration in the follicles after LH surge on the granulosa-lutein cells. Therefore, the this study we evaluated the apoptosis and the production of progesterone $(P_4)$ and estradiol $(E_2)$ in the granulosa-lutein cells that are obtained during oocyte-retrieval after treatment with 5, 50, and $500{\mu}g/m\ell$ cortisol and 1 IU/$m\ell$ FSH. Results of DNA fragment analysis and TUNEL assay demonstrated that DNA fragmentation and the rate of apoptotic cells were increased in a dose-dependent manner showing a significant increase in 50 and $500{\mu}g/m\ell$ cortisol treated cells. We found, however, that FSH did not suppress the apoptosis of the cells induced by cortisol. In the results of chemiluminescence assay for $P_4$ and $E_2$, $P_4$ production was decreased by cortisol treatment, whereas $E_2$ was not changed. We also demonstrated that FSH did not inhibit the suppressive effect of GnRH on $P_4$ production as the result of apoptosis. The present study suggests that cortisol of high concentration could cause the apoptosis of human granulosa-lutein cells by suppressing the production of $P_4$. However, we need more studies to elucidate the mechanism by which cortisol induces apoptosis in human granulosa-lutein cells in view of the fact that our results are inconsistent with previous reported data.

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The Effect of Exam Stress and Vitamin B Complex on Plasma ACTH, Cortisol and Prolactin Level (시험 스트레스와 Vitamin B 복합제가 혈장 ACTH, Cortisol 및 Prolactin치에 미치는 영향)

  • Koh, Kyung-Bong
    • Korean Journal of Psychosomatic Medicine
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    • v.4 no.2
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    • pp.207-214
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    • 1996
  • The study was designed to find out the effect of exam stress and vitamin B complex on hormones such as plasma ACTH, cortisol and prolactin. 21 medical students completed the whole period of the study. Global assessment of recent stress(GARS) scale and SCL-90-R were used to measure stress perception and psychopathology. Radioimmunoassay was used to assess plasma ACTH, cortisol and prolactin. Plasma ACTH level was significantly higher 2 weeks prior to examination and exam period, respectively, than 4 weeks prior to the exam. However, there were no significant differences in plasma cortisol and prolactin level among the three periods. No significant differences were also found in plasma ACTH, cortisol and prolactin level between vitamin and non-vitamin groups during each period. Scores of stress perception in economic area significantly had a positive correlation with plasma ACTH and prolactin level, respectively, 2 weeks prior to the exam. In psychopathology, scores of hostility subscale significantly had a Positive correlation with plasma ACTH level. There were no significant differences in change of each of the hormones over time as well as between vitamin and non-vitamin groups. In conclusion, it was found that ACTH was more sensitive to exam stress than cortisol or prolactin, and that vitamin B complex had no significant influence on ACTH, cortisol and prolactin level.

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Effects of Short-term Thermal Stress on the Mouse Serum Concentrations of Cortisol and Dehydroepiandrosterone Sulphate (단기 고온 스트레스가 마우스 혈청 Cortisol, Dehydroepiandrosterone Sulphate 농도에 미치는 영향)

  • 차정호;최광수;최형송
    • Korean Journal of Animal Reproduction
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    • v.24 no.1
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    • pp.109-113
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    • 2000
  • This study was carried out to investigate the effect of short-term thermal stress on the serum concentrations of cortisol and DHEAS in BALB / c male mice. Cortisol and DHEAS concentrations in serum were measured by radioimmunoassay(RIA). We found there were significantly increased in the cortisol levels in 30 min-stressed group(T30) compared with control group(p<0.01), and then declined without significance in 120 min-stressed group (T120) compared with T30. By contrast, DHEAS levels were decreased without significance in both T30 and T120 compared with control group. Though short-term thermal stress, the continuous decline of DHEAS levels were observed. These results show that short-term thermal stress affects the serum levels of cortisol and DHEAS in mice. Furthermore, we found that DHEAS is a stress-related hormone and will be able to utilize as a stress marker.

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Children's Cortisol Patterning at ChildCare Centers (보육의 특성에 따른 유아의 코티솔 패턴)

  • Park, Kyung Ja;Choi, Jungyoon;Kwon, Yeon Hee;Kim, Jihyun
    • Korean Journal of Child Studies
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    • v.28 no.6
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    • pp.201-215
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    • 2007
  • This study examined cortisol patterning in 160 children(79 boys, 81 girls; aged 4-5) attending twelve childcare centers in Seoul and Kyunggi Province. Saliva samples for the assay of cortisol were collected twice a day at 10 : 30 am and 3 : 30 pm. Saliva samples were collected again within a week after the first collection. Data were analyzed with descriptive statistics and multiple regression analysis. The cortisol level of the afternoon showed neither increase nor decrease compared to morning. Regression analysis indicated that time children spent at childcare centers accounted for increased afternoon cortisol levels; that is, higher levels of afternoon cortisol were associated with more hours per day at childcare centers.

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In vitro Change of IgG Production by Administration of Cortisol Lipopolysaccharide Treated Lymphocyte of Cat (In vitro에서 cortisol투여에 의한 LPS 전처치 고양이 림프구의 IgG생산변화)

  • 나기정
    • Journal of Veterinary Clinics
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    • v.15 no.1
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    • pp.8-13
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    • 1998
  • Change of IgG production of feline mononuclear cell(MNC) was evalual vitro. MNC was treated with lipopolysaccharide(LPS) before cortisol administration. tisol induced change of B cell subpopulation with surface IgG and reduced IgG prods against virus. However, before treatment o$\ulcorner$ MNC with LPS induced increasement of subpopulation with surface IgG and IgG production against virus. These results impel: diminution of IgG production by cortisol is well again by LPS treatment.

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Changes of Stress Hormone Cortisol After Visiting the Gotjawal Forest in Jejudo (제주 교래곶자왈 숲 탐방객의 스트레스 호르몬인 Cortisol량의 변화에 대한 연구)

  • Sin, Bangsik;Im, Dongho;Lee, Keun Kwang
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.10
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    • pp.471-479
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    • 2017
  • The purpose of this study was to investigate changes in salivary cortisol content in 35 subjects before and after visiting Gotjawal Forest in Jeju. Cortisol raw samples were collected before and after visiting Gotjawal Forest and were analyzed by salivary cortisol enzyme immunoassay. The period of the study was from May 1, 2017 to June 30, 2017. There was no significant difference in cortisol content between the control and experimental groups before the visit, but the value of the experimental group significantly decreased afterwards. The mean value of cortisol in male subjects was lower than that of the control group. In the case of women, the value of the experimental group was lower than that of the control group. The post-visit cortisol content of the experimental group was significantly lower than that of the control group. In the post-visit analyses according to gender and group, there were significant differences between genders, groups, and combined effects of gender and group. This study provides basic data to prove the effects of forest bathing.

The Proliferative and Apoptotic Properties of Nile Tilapia (Oreochromis niloticus) sIg+ Lymphocytes by Cortisol Treatment

  • Park, Kwan-Ha;Choi, Sang-Hoon
    • Journal of Animal Science and Technology
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    • v.53 no.6
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    • pp.563-569
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    • 2011
  • The effects of cortisol on proliferation and apoptosis of tilapia surface immunoglobulin positive ($sIg^+$) lymphocytes isolated from different tissues were investigated. $sIg^+$ lymphocytes from the tilapia head kidney (HK) and spleen showed a higher proliferation and lower intracellular calcium ($Ca^{2+}{_i}$) level to Ig-crosslinking compared with peripheral blood $sIg^+$ lymphocytes. Peripheral blood $sIg^+$ lymphocytes stimulated with lipopolysaccharide (LPS) showed high levels of apoptosis in the presence of cortisol. HK and to a lesser extent spleen $sIg^+$ lymphocytes, although less sensitive than their equivalent in peripheral blood, showed cortisol-induced apoptosis irrespective of LPS stimulation of control levels. Compared to plasma values measured during stress conditions, proliferation regardless of LPS stimulation was apparently suppressed by cortisol that is effective in inducing a significant increase in apoptosis in all three different cell populations of $sIg^+$ cells, suggesting the immunoregulatory effect of cortisol in both LPS stimulated and non-stimulated conditions. Different sensitivity of $sIg^+$ cells to the cortisol, in regard to developmental stage and activity, could be related in inhibiting excessive and continuing depletion of $sIg^+$ lymphocytes.

Acupuncture and Cortisol Levels: a Systematic Review

  • Lim, Jin-Woong;Song, Ki-Tae;Lee, Sang-Hoon
    • The Journal of Korean Medicine
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    • v.31 no.6
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    • pp.21-28
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    • 2010
  • Objective: This study was undertaken to systematically assess and summarize the effects of acupuncture on cortisol secretion. Materials and methods: We searched articles published up to May 2010 in six electronic databases (PubMed, Cochrane Library, KISS, KISTI, DBPIA, Kyobo Scholar). Randomized clinical trials (RCTs) which met all the inclusion criteria were reviewed. Eight RCTs were finally selected for this systematic review and assessed by three reviewers. The risk of bias was also estimated by using the Cochrane criteria. Results: Six RCTs reported no distinct difference of cortisol levels between control and experimental groups. Two RCTs reported significant differences of cortisol levels between groups; one reported the acupuncture group with markedly higher concentrations of cortisol while the other reported the opposite result. Conclusion: There are some difficulties in clearly identifying the effects of acupuncture on cortisol levels in this systematic review due to inconsistent results. Therefore, more rigorous trials with larger scales need to be conducted to clarify the effects of acupuncture on cortisol levels.