• Title, Summary, Keyword: Contrast medium

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Replantation of a Traumatically Amputated Penis in a Dog (외상에 의해 절단된 음경의 재접합술 1례)

  • Park, Jin-Uk;Cho, Ki-Rae;Han, Tae-Sung;Choi, Seok-Hwa;Kim, Gon-Hyung
    • Journal of Veterinary Clinics
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    • v.24 no.4
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    • pp.627-630
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    • 2007
  • A mixed-breed hunting dog suffered penile amputation and lacerations in the femoral and inguinal areas while hunting wild boar. The penis was replanted by anastomoses of the urethra, cavernous body, and the left and right dorsal veins of the penis. The transected penis recovered anatomically and functionally. No evidence of postoperative necrosis or edema was detected at the distal portion of the penis. In addition, fistulas and stenosis were not found on urethrogram 20 days after the surgery. Aside from surgery, we performed experimental cavernosography to identify the importance of the anastomosis of the dorsal veins of the penis in three beagle dogs. The cavernosograms revealed that, the contrast medium, which was injected into the bulbus glandis, drained by the left and right dorsal veins of the penis, then converged into one vessel at the ischial arch and diverged into the left and right internal pudendal veins. Thus, reanastomosis of the left and right dorsal veins of the penis in cases of transected canine penis appears to be important for positive postoperative prognosis.

The usability of the MR Breast perfusion image and Time-Signal Intensity curve in Breast cancer patients (유방암 환자에서 MR Breast perfusion 영상과 시간-신호강도 곡선의 유용성)

  • Cho, Jae-Hwan;Lee, Hae-Kag
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.9
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    • pp.4068-4074
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    • 2011
  • The purpose of this study was to examine the usefulness of MR Breast perfusion image and time-signal intensity curve in patients diagnosed with breast cancer. We selected on 20 patients who were histologically diagnosed to have invasive ductal carcinoma (IDC) from March 2009 to December 2010. First, the Breast perfusion mapping image was reconstructed after obtaining the dynamic contrast enhancement image. The reconstructed image measured the slope, maximal relative enhancement, and time to peak on the detail including the lesion region, normal region, back ground region after obtaining the time-signal intensity curve. The lesion region and normal and slope of the back ground part were measured with the quantitive analytical method about the research and the average was compared and was analyze. In the qualitative analysis, the signal strength of each pixel was analyze with the macroscopic and being high it was low, the medium (2) performed the division of (a) by the three-point standard and the average was measured. The findings from the quantitative image analysis are the following: In the lesion region, the slope and maximal relative enhancement were the highestest among and the time to peak was the highestest in the back ground region. In the qualitative analysis, the breast perfusion image showed a diagnostic efficiency.

EFFECT OF DEXAMETHASONE CONCENTRATIONS ON OSTEOGENIC ACTIVITY OF CULTURED HUMAN PERIOSTEAL-DERIVED CELLS (배양된 인간 골막기원세포의 조골활성에 대한 덱사메타손 농도의 효과)

  • Kim, Jong-Ryoul;Park, Bong-Wook;Lee, Chang-Il;Hah, Young-Sool;Kim, Deok-Ryong;Cho, Yeong-Cheol;Sung, Iel-Yong;Byun, June-Ho
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.31 no.4
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    • pp.287-293
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    • 2009
  • Long-term treatment with glucocorticoid leads to the development of osteoporosis and osteonecrosis. In contrast to the marked inhibitory effect of pharmacological doses of glucocorticoids on bone formation, the relationship between physiological concentrations of glucocorticoids and osteoprogenitor cell proliferation and phenotypes has not been elucidated yet. In addition, the effects of dexamethasone treatment on the proliferation and osteoblastic differentiation of osteoprogenitor cells are also controversial. The purpose of this study was to examine the effects of dexamethasone on the proliferation and osteoblastic differentiation of periosteal-derived cells. Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the cells were further cultured for 21 days in the osteogenic induction medium with different dexamethasone concentrations of 0, 10, and 100 nM. The proliferation and osteoblastic phenotypes of periosteal-derived cells were promoted in dexamethasone-treated cells than in untreated cells. Among the dexamethasone-treated cells, cell proliferation was slightly greater in 10 nM dexamethasone-treated cells than in 100 nM dexamethasone-treated cells. Histochemical staining and the bioactivity of alkaline phosphatase (ALP) were higher in 100 nM dexamethasone-treated cells than in 10 nM dexamethasone-treated cells. Similarly, von Kossa-positive mineralization nodules and calcium content were also more evident in 100 nM dexamethasone-treated cells than in 10 nM dexamethasone-treated cells. These results suggest that dexamethasone enhances the in vitro osteoblastic differentiation of periosteal-derived cells. The present study also demonstrates that higher dexamethasone concentrations reduce the in vitro proliferation of periosteal-derived cells.

Isolation and Identification of a Streptomyces sp. that Produces Antibiotics Against Multidrug - Resistant Acinetobacter baumannii (다제내성 Acinetobacter baumannii의 생장을 억제하는 항생물질을 생산하는 방선균의 분리.동정 및 항균효과)

  • Rhee, Ki-Hyeong
    • Microbiology and Biotechnology Letters
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    • v.39 no.1
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    • pp.37-42
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    • 2011
  • I isolated the actinomycete strain KH223 from soil samples collected from the Kye Ryong mountain area. This strain is antagonistic to the multidrug-resistant Acinetobacter baumannii. KH223 was confirmed as belonging to the genus Streptomyces based on the scanning electronmicroscopy(SEM) observations of the diaminopimelicacid(DAP) type and morphological and physiological characteristics. Comparison of the 16S rDNA nucleotide sequences revealed that KH223 has a relationship with Streptomyces galbus. Production of antibiotics by KH223 was most favorable when cultured on a glucose, polypeptone, and yeast extract(PY) medium for 6 days at 27$^{\circ}C$. The supernatant was found to exhibit an antimicrobial effect on various kinds of bacteria and fungi. Particularly, butanol and ethylacetate extracts of KH223 and cyclo(trp-trp) exhibited significant activity against A. baumannii at concentration ranges of 0.8-12.5 ${\mu}g$/mL, 5.0-25 ${\mu}g$/mL and 12.5${\rightarrow}$100 ${\mu}g$/mL, respectively. Moreover, in contrast to cyclo(trp-trp) had shown to activity against Micrococcus luteus JCM 1464 at the concentration of 12.5 ${\mu}g$/mL, the butanol extract of KH223 showed significant activity against Bacillus subtilis IAM 1069 and Micrococcus luteus JCM 1464 at the concentration of 0.4 and 0.8 ${\mu}g$/mL, respectively. These results suggest that KH223 may have a great potential in the production of new antibiotics to combat multidrug-resistant pathogens and further studies may be warranted for the same.

The Contents of Emergency Treatment Practice Conducted by Emergency Medical Technician and Related Factors (일부 응급구조사들의 응급처치 시행내용과 관련 요인)

  • Jeong, Sang-Gil;Kim, Ki-Soon;Cho, Soo-Hyung;Kang, Myung-Geun;Han, Mi-Ah
    • Journal of agricultural medicine and community health
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    • v.34 no.3
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    • pp.346-358
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    • 2009
  • Objectives: A study was conducted by analysing the contents and frequency of emergency treatment practiced by emergency medical technicians(EMTs) in emergency situations and contains related factors. Methods: The study subjects included 80 EMTs who participated in ongoing education classes during the period from June 25th to June 26th, 2008. the survey included 53 EMTs employed within 119 first aid teams in fire stations in both a metropolitan city and a medium-sized city. From the survey results relation between frequency of emergency treatment and subjects characteristics explored statistically including t-test, X2 test, Pearson correlation and finally stepwise multiple regression. Results: The most frequent procedure by EMTs checking vital signs. The statistically significant related factors overall of the 23 items of emergency treatment frequency was the working area and the grading of EMT certification. In contrast, significant related factors with frequency of IV linkage were found to be the subjects gender, institution employed and the importance of recognizing the item. The statistically significant related factors of that of ECG monitoring was the gender of respondents. Conclusions: To keep the appropriate frequency of emergency treatment by EMT, active efforts to promote their individual importance and confidence cognition are necessary, especially towards males and persons who have 2nd class certification. Moreover, EMTs who work in small cities must be supported.

Supply of Tryptophan and Tryptamine Influenced the Formation of Melatonin in Viola Plants (제비꽃속(Viola) 식물에서 tryptophan과 tryptamine 공급이 멜라토닌 생성에 미치는 영향)

  • Kim, Yeo-Jae;Yoon, Young-Ha;Park, Woong-June
    • Journal of Life Science
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    • v.21 no.2
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    • pp.328-333
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    • 2011
  • Melatonin has been known as an animal hormone. However, melatonin exists in diverse organisms including higher plants. The biosynthesis and physiological roles for melatonin in plants is still largely unknown, although both dicot and monocot plants have melatonin and some medicinal plants even contain large amounts of melatonin. In this study we detected melatonin in diverse Viola plants, in which melatonin had not been examined so far, by reverse phase HPLC analysis, demonstrating the wide existence of melatonin in the genus of Viola. We then fed tryptophan (Trp) and tryptamine (TAM) to the incubation medium for Viola leaf sections to test their effects on melatonin formation. Trp is also the hypothesized starting material of melatonin in plants, and TAM is the following intermediate produced by the decarboxylation of Trp. Trp feeding did not affect the contents of melatonin. In contrast, TAM feeding clearly increased the level of melatonin in Viola leaves. Because TAM is derived from Trp, we concluded that the Trp-TAM pathway exists in Viola plants as well. Ineffectiveness of Trp feeding to the change of melatonin contents supports the hypothesis that the decarboxylation step from Trp to TAM is the rate-limiting step in plant melatonin biosynthesis.

Effects of ABA, reduced nitrogen source and osmoticum for somatic embryogenesis in Liriodendron tulipifera (백합나무의 체세포배 유도에 미치는 ABA, 환원질소원 및 삼투압제 효과)

  • Kim, Yong-Wook;Han, Mu-Seok;Moon, Heung-Kyu;Park, So-Young
    • Journal of Plant Biotechnology
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    • v.38 no.2
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    • pp.186-190
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    • 2011
  • This study was conducted to evaluate effects of various kinds or concentrations in abscisic acid (ABA), reduced nitrogen sources (casein hydrolysate, casamino acid and L-glutamine) and osmoticum for production of somatic embryos (SEs) from pro-embryogenic mass (PEM) in yellow poplar (Liriodendron tulipifera). In comparison of various concentrations of ABA, the highest number (640/10 mg PEM) of SEs was marked in the treatment of 0.5 mg/L. With higher concentration than 0.5 mg/L ABA, number of induced SEs were decreased. And the lowest number of SEs were obtained from the treatment of 20 mg/L ABA. Differences of 8 treatments of the nitrogen sources in the medium were also compared. In the experiment of 8 treatments for SEs production, the highest result showed in the treatment of 500 mg/L casamino acid (223/5 mg PEM). In comparison of different kinds/concentrations of osmotica for SEs induction, the best response was obtained from the treatment of 4% sucrose (317/5 mg PEM). In contrast, no SEs were found from the treatments supplemented with any concentrations of maltose.

Stress-induced biphasic ethylene and ROS biosynthesis are synergistically interacted in cell damage (스트레스에 의한 식물세포 손상에서 Biphasic Reactive Oxygen Species(ROS)와 Ethylene 생합성의 Synergism 효과)

  • Ji, Na-Ri;Park, Ky-Young
    • Journal of Plant Biotechnology
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    • v.38 no.1
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    • pp.22-29
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    • 2011
  • Although reactive oxygen species (ROS) are inevitable by-products of many redox reactions in eukaryotic cells, they play a crucial role as signaling molecules in many cellular processes for development and defense response to abiotic stresses. The biphasic ROS production which was peaked twice in a first transient phase and a second massive phase was occurred after treatment of abiotic stress such as oxidative stress, high salinity. This biphasic generation of ROS was followed by the biphasic production of stress hormone, ethylene. The mechanism of interactions between ROS and ethylene biosynthesis is studied in tobacco (Nicotiana tabaccum L.) plants under the abiotic stresses. The stress-induced ethylene production was significantly inhibited in RbohD-AS and RbohF-AS, in which antisense expression of NADPH oxidase genes was performed. The accumulation of ROS, which was determined by DAB and DCFH-DA staining, was significantly decreased after abiotic stresses in transgenic plants. The suppression of signaling with ethylene and ROS induced more tolerance in response to abiotic stress. The transgenic plants were more tolerant in MS medium supplemented with salinity stress in contrast with wild-type. Stress-induced cell damage determined by DNA fragmentation was decreased at phase II in those transgenic plants. Therefore, the first burst of ROS is more responsible for making a role as a signaling molecule during stress-induced response. These results suggested that ethylene and ROS act in a positive feedback cycle that results in mutual enhancement of ethylene and ROS production during stress-induced cell death.

Procambium differentiation and shoot apical meristem development in somatic embryos of soybean (Glycine max L.) (대두 체세포배에서 전형성층 분화와 경단분열조직의 발달)

  • Choi, Pil Son;Kwon, Suk Yoon
    • Journal of Plant Biotechnology
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    • v.40 no.1
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    • pp.55-58
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    • 2013
  • Immature embryos of Glycine max L. was cultured on Murashige and Skoog's (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). After 6 to 8 weeks of culture, immature embryos produced somatic embryos. Of somatic embryos, two cotyledonary embryo (14%), one cotyledonary embryo (37%), fused cotyledonary embryo (43%), and stunted globular embryos (6%) were observed. The procambial strand of cotyledons originated from circular procambial tissues of lower hypocotyl. The circular procambial tissues were independently divided into one or two procambial strand at the edge of cotyledonary-node, and then connected to each cotyledon to form somatic embryos with one or two cotyledons. When cotyledon was a fused type, the circular procambial strand in lower hypocotyl was continuously connected to the cotyledon. Also, somatic embryos with two cotyledons developed a functional shoot apex with the tunica-corpus structure. In contrast, somatic embryos with one or fused cotyledon formed an abnormal shoot apex without the tunica-corpus structure or with non-dome shape in the inter-cotyledonary area. These results indicated that the variation of cotyledon in somatic embryos is closely related to procambial differentiation and shoot apical meristem development.

Effect of Deep Seawater on Expression of μ-Opioid Receptor in Cultured Rat Hippocampal Neurons (배양된 쥐 해마신경세포에서 μ-아편양 수용체의 발현에 대한 해양심층수의 영향)

  • Moon, Il-Soo;Kim, Seong-Ho
    • Journal of Life Science
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    • v.21 no.2
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    • pp.176-182
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    • 2011
  • Deep seawater (DSW) generally refers to seawater at depths equal to or greater than 200 meters. DSW is rich in inorganic materials which have attracted attention for its various applications. In this study we investigated the effects of the DSW upwelled from the East Sea, offshore Yang Yang (KangWon-do, Korea), on the expression of ${\mu}$-opioid receptor (MOR) of cultured rat hippocampal neurons. Neurons were grown in a minimal essential medium containing 10% (v/v) fetal bovine serum and either 25% (v/v) distilled water, or hardness (H) 800, or H 1000 DSW. Cultures grown in the presence of DSW with H 800 and H 1000 exhibited robust MOR immunoreactive signals in both neurons and astrocytes. Interestingly, the increase in MOR immunoreactive signals was more dramatic in astrocytes than in neurons. Statistical analysis revealed that the relative intensities for MOR clusters increased approximately 4-fold in astrocytes cultured in H 800 and H 1000 media. These increases were statistically very significant (p<0.001). In contrast, the increase in intensities for MOR immunoreactive signals was relatively less dramatic in neurons, where only the increase in the H 1000 culture was statistically very significant (p<0.001). These results indicated that DSW promotes expression of MOR in both neurons and astrocytes, and more significantly in the latter.