• Title/Summary/Keyword: Chryseobacterium

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Production Properties on Extracellular Protease from Chryseobacterium Novel Strain JK1 (Chryseobacterium 속 신종세균 JK1의 세포외 단백질분해효소 생산특성)

  • Lee, Yu-Kyong;Oh, Yong-Sik;Roh, Dong-Hyun
    • Korean Journal of Microbiology
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    • v.48 no.1
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    • pp.48-51
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    • 2012
  • A novel Chryseobacterium sp. JK1 strain producing extracellular protease had been isolated from soil. The largest clear zones were observed on nutrient agar plates supplemented with 1% skim milk at $30-35^{\circ}C$ along with the growth of Chryseobacterium sp. JK1. The cell growth of JK1 strain was maximal at 24 h and maximum protease activity was reached up to 560 unit/ml at the stationary phase in liquid culture. In the presence of maltose, glucose or mannitol in Nutrient broth, cells grew well, but protease were produced poorly with lower production yields of 64-77% than in NB broth only. Similarly, the addition of skim milk, beef extract, yeast extract, malt extract or tryptone showed good growth and poor enzyme production. On the contrary, the addition of $(NH_4)_2HPO_4$ or $(NH_4)_2SO_4$ gave poor growth and good enzyme production of 121-146%.

Isolation, Identification, and Characterization of a Keratin-degrading Bacterium Chryseobacterium sp. P1-3

  • Hong, Sung-Jun;Park, Gun-Seok;Jung, Byung Kwon;Khan, Abdur Rahim;Park, Yeong-Jun;Lee, Chang-Hyun;Shin, Jae-Ho
    • Journal of Applied Biological Chemistry
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    • v.58 no.3
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    • pp.247-251
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    • 2015
  • In this study, a keratin-degrading bacterium was isolated from soil contaminated with feather waste. The isolated strain was identified as Chryseobacterium sp. P1-3 on the basis of the 16S rRNA gene sequence alignment. Chryseobacterium sp. P1-3 is currently used in various biotechnological applications (e.g., in the hydrolysis of poultry feathers). It hydrolyzed the feather meal within 2 days and possesses a high level of keratinase activity (98 U/mL). The keratinase, partially purified from this strain, prefers casein as a substrate and shows optimal activity at a temperature of $30^{\circ}C$ and at a pH of 8.0.

Characterization of Chryseobacterium aquaticum Strain PUPC1 Producing a Novel Antifungal Protease from Rice Rhizosphere Soil

  • Gandhi Pragash, M.;Narayanan, K. Badri;Naik, P. Ravindra;Sakthivel, N.
    • Journal of Microbiology and Biotechnology
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    • v.19 no.1
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    • pp.99-107
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    • 2009
  • Strain PUPC1 produces an antifungal protease as well as plant growth promoting enzymes such as 1-aminocyclopropane-1-carboxylate (ACC) deaminase and phosphatase. Morphological, cultural, and physiological characteristics as well as 16S rRNA gene-sequence-based phylogenetic analysis confirmed the taxonomic affiliation of PUPC1 as Chryseobacterium aquaticum. The optimum growth of PUPC1 was observed at pH 6.0 and $30^{\circ}C$, and maximum protease production was observed in medium B amended with 1% tryptone, 0.5% sucrose, and 0.005% $MnCl_2$. The protease was purified by ammonium sulfate precipitation, Sephadex G-75 gel filtration chromatography, and electroelution from preparative SDS-PAGE. The protease had a molecular mass of 18.5 kDa. The optimum pH and temperature stability of the protease were pH 5.0-10.0 and temperature $40-70^{\circ}C$. Chryseobacterium aquaticum PUPC1 and its protease showed a broad-spectrum antifungal activity against phytopathogenic fungi. Strain PUPC1 also exhibited plant growth promoting traits. The objective of the present investigation was to isolate a strain for agricultural application for plant growth promotion and biocontrol of fungal diseases.

Two cases of Chryseobacterium meningosepticum infection in a neonatal intensive care unit (신생아 중환자실에서 발생한 Chryseobacterium meningosepticum 감염 2례)

  • Yoon, Hye Sun
    • Clinical and Experimental Pediatrics
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    • v.50 no.7
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    • pp.698-701
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    • 2007
  • We report on two premature infants who developed nosocomial infection caused by Chryseobacterium meningosepticum in a neonatal intensive care unit (NICU). One premature infant developed sepsis, meningitis, and hydrocephalus, and was treated successfully with ciprofloxacin plus trimethoprim-sulfamethoxazole combination therapy for 4 weeks and with a ventriculoperitoneal shunt. The other premature infant, who was in a chronically debilitated state, had infection that had colonized only in the respiratory tract but had no clinical signs for 66 days. Extensive environmental surveillance demonstrated that the suction bottle apparatus was the source of infection. We prevented the spread of infection by closing the NICU temporarily, isolating the patients early in their infection, and eradicating the source of infection source.

Isolation and Characterization of Keratinolytic Protein Chicken Feather-Degrading Bacteria (난분해성 케라틴 단백질을 함유하는 닭 우모 분해세균의 분리 및 특성)

  • Kim, Se-Jong;Cho, Chun-Hwi;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.46 no.1
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    • pp.86-92
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    • 2010
  • Thirty-one chicken feather-degrading bacteria were isolated from wasted feather, compost and wastewater in a chicken farm. These isolates were categorized as Firmicutes (21 strains), ${\gamma}$-proteobacteria (4 strains), Actinobacteria (4 strains), and Bacteroidetes (2 strains) by 16S rRNA gene sequence analysis. We examined the feather-degrading isolates for degradation in the 2% of chicken feather meal. The strain Chryseobacterium sp. FBF-7, Stenotrophomonas maltophilia FBS-4, and Lysinibacillus sp. FBW-3 were selected as a keratinolytic protein degrading bacteria which showed the highest feather degradation of 75-90%. The characteristics of amino acids extracted from chicken feather meal by using keratinolytic protein degrading isolates and chemical method with $Ca(OH)_2$ were analyzed. Total amino acid content of strain Chryseobacterium sp. FBF-7 was 1,661.6 ${\mu}mol$/ml, which was the highest and it was similar with chemical method. And essential amino acid content of total amino acid was thirty-seven percent (619.3 ${\mu}mol$/ml) and 596.9 ${\mu}mol$/ml for keratinolytic protein degrading isolates and chemical method, respectively. The major amino acids were valine, glutamic acid, aspartic acid, glycine, and proline by the strain Chryseobacterium sp. FBF-7 and especially, higher contents of aspartic acid, threonine, serine, cysteine, and tyrosine were detected compared with chemical method.

Characterization of Extracellular Protease Secreted from Chryseobacterium sp. JK1 (Chryseobacterium sp. JK1이 분비하는 세포외 단백질분해효소 특성)

  • Lee, Yu-Kyong;Oh, Ji-Sung;Roh, Dong-Hyun
    • Korean Journal of Microbiology
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    • v.49 no.1
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    • pp.78-82
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    • 2013
  • A novel Chryseobacterium sp. JK1 strain isolated from soil had been reported that this isolate produced large amount of extracellular protease at mesophilic temperature in previous study. The optimal temperature and pH of extracellular protease were $40^{\circ}C$ and 7.0, respectively, showing narrow range of optimal temperature and relatively broad activity from pH 6.0 to 9.0. In addition, the protease showed greatest activity against skim milk and lowest against bovine serum albumin (BSA). The protease strongly inhibited by ethylenediaminetetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA) or phenylmethylsulfonyl fluoride (PMSF), and addition of cation $Ag^+$ or $Cu^{2+}$, and slightly inhibited by $Al^{3+}$. No significant inhibition was found with pepstatin, and addition of cation, $K^+$, $Ca^{2+}$, $Na^+$, $Fe^{2+}$ or $Mg^{2+}$. On the contrary, protease was enhanced by addition of divalent cation $Mn^{2+}$ (5 mM). Zymography analysis of concentrated culture supernatant revealed two major bands at 67 and 145 kDa. These results suggest that Chryseobacterium sp. JK1 strain produced extracellular neutral serine proteases which could apply in food industry.

Complete genome sequence of Chryseobacterium sp. T16E-39, a plant growth-promoting and biocontrol bacterium, isolated from tomato (Solanum lycopersicum L.) root (토마토 뿌리에서 분리한 식물생육촉진과 생물방제 세균 Chryseobacterium sp. T16E-39 균주의 유전체 서열)

  • Lee, Shin Ae;Kim, Sang Yoon;Sang, Mee Kyung;Song, Jaekyeong;Weon, Hang-Yeon
    • Korean Journal of Microbiology
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    • v.53 no.4
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    • pp.351-353
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    • 2017
  • Chryseobacterium sp. strain T16E-39, isolated from roots of a tomato plant, promotes plant growth and suppresses phytophthora blight and bacterial wilt diseases. The complete genome of strain T16E-39 consists of a circular chromosome with 4,872,888 base pairs with a G + C content of 35.22%. The genome includes 4,289 coding sequences, 15 rRNAs, and 71 tRNAs. We detected genes involved in phosphate solubilization, phytohormone regulation, antioxidant activity, chitin degradation, and the type IX secretion system (T9SS) that may be related to growth promotion and disease suppression in plants.

Genetic Diversity of Metallo-β-lactamase Genes of Chryseobacterium indologenes Isolates from Korea

  • Yum, Jong Hwa
    • Biomedical Science Letters
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    • v.25 no.3
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    • pp.275-281
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    • 2019
  • This study was performed to characterize the chromosomal metallo-${\beta}$-lactamases (MBLs) of Chryseobacterium indologenes isolated from Korea and to propose a clustering method of IND MBLs based on their amino acid similarities. Chromosomal MBL genes were amplified by PCR from 31 clinical isolates of E. indologenes. Nucleotide sequencing was performed by the dideoxy chain termination method using these PCR products. Antimicrobial susceptibilities were determined by the agar dilution method. PCR experiments showed that all 31 E. indologenes isolates contained all $bla_{IND}$ genes. DNA sequence analysis revealed that E. indologenes isolates possessed ten types of $bla_{IND}$ gene, including seven novel variants ($bla_{IND-8}$ to $bla_{IND-14}$). The most common combination of MBL was IND-2 (n = 18). Minimum inhibitory concentrations of imipenem and meropenem for the isolates harboring novel IND MBLs were ${\geq}16{\mu}g/mL$. IND MBLs were grouped in three clusters, based on amino acid similarities.

Isolation of Chryseobacterium meningosepticum Producing $\beta$-Mannosidase from a Mudfish (미꾸라지로부터 $\beta$-Mannosidase를 생산하는 Chryseobacterium meningosepticum의 분리)

  • Kim, Hyeon-Suk;Yun, Gi-Hong
    • Microbiology and Biotechnology Letters
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    • v.32 no.4
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    • pp.371-374
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    • 2004
  • A bacterium producing the $\beta$-mannosidase was isolated from intestine of fresh fish. The isolate YB-29 has been identified as Chryseomeningosepticum on the basis on its 16S rRNA sequence, morphology and biochemical proper The $\beta$-mannosdiase activity was detected in both the culture filtrate and the cell extract of C. meningosepticum YB-29. The $\beta$-mannosidase of culture filtrate showed the maximum activity for hydrolysis of para-nitrophenyl-$\beta$-D-mannopyranoside at pH 5.0-6.0 and 55-$60^{\circ}C$. The enzyme was able to hydrolyze the oligomeric substrates such as mannobiose and mannotriose with higher activity for mannotriose than mannobiose.

Identification of an Antagonistic Bacterium, KJ1R5, for Biological Control of Phytophthora Blight of Pepper

  • Kim, Hye-Sook;Myung, Inn-Shik;Kim, Ki-Deok
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • pp.97.1-97
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    • 2003
  • An antagonistic bacterium, KJ1R5,, to Phytophthora capsici was obtained from root interior of a healthy pepper plant. To identify the bacterial antagonist, 16S rDNA sequence analysis, Biolog system, fatty acid methyl-esters (FAMEs), and physiological and biochemical characterization were conducted. The determined 165 rDNA sequence of KJ1R5, showed higher similarities to those of a group consisting of several Chryseobacterium strains with 95.2, 95.2, and 95,1% similarity to C. defluvii, Chryseobacterium sp. FR2, and C. scophthalmum, respectively, In addition, Halounella gailinarum, Bergeyella zoohelcum, and Riemerella anatipestifer are another group for KJ1R5, with 94.1, 89.7, and 87.2% similarities, respectively When identification of the antagonistic bacterium, KJ1R5, was conducted using BIOLOG system, the strain KJ1R5, was identified as Flavobacterium tirrenicum (similarity; 0.75%). Fatty acid profiles of the strain KJ1R5, were composed mainly of iso-17:0 w9c and iso-15:0 and identified as Chryseobacterium balustinum (similarity 0.524%). KJ1R5, was Gram-negative, regular short rods ranging from 0.8 $\mu\textrm{m}$ to 1.0 $\mu\textrm{m}$ and had no flagella. Phenotypic characterization of the antagonistic bacterium indicated that KJ1R5, were included in the genus Chreseobacterium, which belongs to the family Flavobacteriaceae. The strain was distinguished from these six existing species. These results indicated that strain might be placed as a new species in the genus Chryseobacterium.

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