• Title, Summary, Keyword: Chick

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Effects of Radioactive Phosphorus (32P) on the Growth of Chick Embryo and Young Chick (방사성(放射性) 인(燐) (32P)이 계태아(鷄胎兒) 및 초생추발육(初生雛發育)에 미치는 영향(影響))

  • Lee, Joon Sup;Yoon, Suk Bong;Ko, Kwang Doo
    • Korean Journal of Veterinary Research
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    • v.11 no.2
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    • pp.117-122
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    • 1971
  • This experiment was performed to study the effects of radioactive phosphorus($^{32}p$) on the growth of chick embryo and young chick. Radioactive phosphorus($^{32}p$) was administered into the yolk sac of chick embryo in doses of 2 uci/gm and 1 uci/gm and was administered intraperitoneally to the young chick in doses of 1 uci/gm and 0.5 uci/gm. The chick embryos were sacrificed on 6th, 7th, 8th, 9th and 10th day and the chicks were sacrificed on 1st, 3rd, 6th and 10th day after the administration and the liver, kidney, spleen, brain, eye ball and femur were weighed to observe the effects of growth inhibition on them. The results obtained were as follows. 1. A marked growth inhibitory effect was found on 8th, 9th and 10th day after the administration of $^{32}p$ in chick embryos and the same effect was found on 6 th and 10 th day after the administration in chicks. 2. The growth of the liver, kidney, spleen and femur was inhibited markedly but the brain and eye ball were not affected in chick embryos and chicks.

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Ultrastructure and Dehydrogenase activity on the Differentiation of the Cerebral Nerve Cell in the Chick Embryo (1) (계배 대뇌의 신경세포 분화에 따른 탈수소효소 활성 및 미세구조 (1))

  • Kim, Saeng-Gon
    • Applied Microscopy
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    • v.28 no.4
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    • pp.563-575
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    • 1998
  • To investigate the changes during the differentiation of the cerebral neurons of chick embryo of tne embryogenic day (ED) 7 and 8, the ultrastructural changes in the cerebral neurons, the activity of dehydronases (LDH, MDH and SDH), protein expression profile and adenosine triphosphate concentration were analyzed. In ED 7 chick embryos, relatively large nucleus, centrally located nucleolus, evenly spread chromatin over nucleoplasm, and prominent nuclear envelope were observed. Oval-shaped mitochondria with well-developed cristae were present over entire cytoplasm. In ED 8 chick embryos, evenly spread chromatin over nucleoplasm, and prominent nuclear envelope were observed. In the cytoplasm, well-developed rough endoplasmic reticulum and Golgi complex were observed. In ED 7 chick embryos and ED 8 chick embryos, 31 polypeptide bands and 34 polypeptide bands were observed, respectively. The activities of dehydrogenases were lower in ED 7 chick embryos than in ED 8 chick embryos. LDH activity was 8.16 (ED 7) and 9.28 (ED 8), MDH activity was 7.98 (ED 7) and 10.10 (ED 8), and SDH activity was 5.49 (ED 7) and 7.14 (ED 8) respectively. The ATP concentration remained unchanged over ED 7 and 8.

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Identification of Chromosomal Band Marker of Korean Native Chick by High-resolution Banding Technique (고분염분석법(High-resolution banding)에 의한 한국재래계의 염색체 분염 표지 설정)

  • 백규흠;손시환
    • Proceedings of the Korea Society of Poultry Science Conference
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    • pp.85-87
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    • 2000
  • To obtain the genetic information of Korean native livestock, the karyotyping of Korean native chick were performed by high-resolution banding technique. The chromosomes were prepared from lymphocyte culture and early embryos with 200 Korean native chick which have been raised at National Livestock Research Institute. There were no significant difference between Korean native chick and Leghorn in the number of chromosomes and chromosomal morphological pattern. Using high resolution banding technique, the yield of G-bands of prophase is much greater than that can be obtained by International System for Standardzed Avian Karyotypes(ISSAK, 1999). The G-band landmarks of Korean native chick were similar to those of ISSAK and Leghorn except some macrochromosomes. chromosome Z and 3 had C-band variants with heteromorphic patterns on distal and centromeric site. The proportion of constitutive heterochromatin, the heterochromatin ratio of Korean native chick was significantly more than that of Leghorn in all chromosomes.

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The ABC in Chick Lit: the Consumption of Asian America in The Dim Sum of All Things

  • Chung, Hyeyurn
    • English & American cultural studies
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    • v.18 no.1
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    • pp.53-92
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    • 2018
  • This essay aims to examine chick lit written within the Asian American context. For the most part, the chick lit genre has been typically regarded as a site to study contemporary white women's experiences and to debate the genres' credentials as feminist literature. Though some may disagree, there is general consensus that chick lit has fallen out of vogue after reaching its peak in the first decade of the new millenium.; nevertheless, it is being revisited by readers and critics alike as it has recently re-emerged as a location upon which to examine how race and gender inform notions of national belonging and female subject formation in the twenty-first century. To this end, this essay reads Kim Wong Keltner's The Dim Sum of All Things (2004). Keltner's protagonist Lindsey Owyang is yet another twentysomething "chick" looking for love, self, independence, and success in the huge megalopolis of San Francisco. What sets Lindsey apart from the chick prototype is that she is a third-generation ABC (American-born Chinese) and issues relevant to Asian America frequently make their way into Lindsey's narrative. Though it is generally considered as standing a "few notches above the standard chick-lit fare" (Stover n. pag), I would argue that meaningful reflections on many of the major pillars of Asian American literature, history, and cultural politics are glossed over in favor of cursory musings about the daily vicissitudes of Lindsey's life. This essay thus takes to task Ferriss's claim that a "serious" consideration of chick lit "brings into focus many of the issues facing contemporary women and contemporary culture - issues of identity, of race and class, of femininity and feminism, of consumerism and self-image" (2). I contend that a close examination of Keltner's The Dim Sum of All Things discloses that the chick lit format undermines a "serious consideration" of Asian American issues by presenting in particular a highly problematic representation of race and of Asian American femininity.

Studies on the Effect of Ginseng Extract on Chick Embryonic Nerve and Muscle Cells (인삼이 신경 및 근육 세포에 미치는 영향에 대한 연구)

  • 김영중;김은경
    • YAKHAK HOEJI
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    • v.24 no.3_4
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    • pp.143-150
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    • 1980
  • The effect of ginseng saponin on chick embryonic dorsal root ganglia organ culture and brain, spinal cord, muscle dissociation cultures was studied. The fiber outgrowth in explanted chick embryonic dorsal root ganglia was markedly induced by water and alcohol extracts of ginseng, total ginseng saponin, protopanaxadiol and protopanaxatriol glycosides as well as ginsenosides R/sub b1/, R/sub d/, R/sub 0/+R/sub a/+R/sub b1/, and R/sub b2/+R/sub c/+R/sub e/ mixtures. The life span of the cultured chick embryonic dorsal root ganglia and potentiation of nerve cell density were also observed with all of these ginseng saponins. The effect of ginseng saponin on chick embryonic dorsal root ganglia organ culture was more marked in the absence of the chick embryonic extract which was known to contain nerve growth factor-like material in the culture media. However, the ginseng saponin did not influence the cultured central nervous system such as brain and spinal cord cells and cultured skeletal muscle cells with respect to the morphological changes, maturation and life span of these cells.

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Avian Somitic Cell Chimeras Using Surrogate Eggshell Technology

  • Mozdziak, Paul E.;Hodgson, Dee;Petitte, James N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.6
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    • pp.801-806
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    • 2008
  • A classical technique to study somitic cell fate is to employ the cross-transplantation of quail somites into a chick host. The densely stained nucleoli of the quail cells makes it possible to assess the fate of the donor quail cells in the chick host. Classical somite transplantation techniques have been hampered by the necessity of a small opening in the chick eggshell, difficulty in hatching the offspring and interspecies post-hatch graft rejection. With the advent of transgenic chicken technology, it is now possible to use embryos from transgenic chickens expressing reporter genes in somite cross-transplantation techniques to remove any possibility of interspecies graft rejection. This report describes using a surrogate eggshell system in conjunction with transgenic chick:chick somitic cell cross-transplantation to generate viable chimeric embryos and offspring. Greater than 40% of manipulated embryos survive past 10 days of incubation, and ~80% of embryos successfully cultured past 10 days of incubation hatched to produce viable offspring.

Effects of Supplemention of Antibiotic, Probiotic and Yeast Culture of Performance and Meat Quality in Broiler Chicks (항생제, 생균제 및 효모제 첨가가 육계의 성장과 육질에 미치는 영향)

  • 박성진;유성오
    • Korean Journal of Poultry Science
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    • v.27 no.3
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    • pp.203-208
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    • 2000
  • The present study was conducted to investigate the effects of dietary supplementions of 0.1% probiotic and 0.1% yeast culture on the growth performance and meat quality of broiler chicks. A total of 160 Arbor Acre broiler chicks were randomly allotted to 16 pens ; four pens per treatment and 10 birds per pen. Feeding trial lasted for 6 weeks. The results obtained are summerized as follows : The body weight gain and feed intake was slightly higher in group of broiler chick supplemented antibiotic than those of other treatment groups, but were not significantly different among treatments. The feed conversion were slightly lower in groups of broiler chick supplemented antibiotic and probiotic than those of other treatment groups, but were not significantly different among treatments. The pH of thigh musle was slightly higher in group of broiler chick supplemented probiotic than those of other treatment groups. Crude protein, crude fat and crude ash of thigh muscle were lower in groups of broiler chick supplemented probiltic and yeast culture than those of other treatment groups, but were not significantly different among treatments. The saturated fatty acid content of the thigh muscle was tende to be higher in group of broiler chick supplemented yeast culture, but the unsaturated fatty acid content was tende to be lower in group of broiler chick supplemented yeast culture than those of other treatment groups.

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Cell Biological Studies of the EfEect of Serotonin on Chick Embryogenesis (초기계배 발생에 미치는 Serotonin의 영향에 관한 세포생물학적 연구)

  • 최임순;주충노
    • The Korean Journal of Zoology
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    • v.30 no.4
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    • pp.432-444
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    • 1987
  • Very early stage of chick embryo cultivated in the medium containing DLtryptophan by beaker method has been investigated in vitro morphologically using electron microscopy at cellular level and found that the development of tryptophan treated chick embryos corresponding to 18∼66 hrs incubation was impaired and york granule degradation was significantly delayed. It was also found that DNA, RNA and protein biosynthesis of tryptophan treated chick embryo was greatly lowered than those of control group. Conversion of L-tryptophan into serotonin was traced using 14C-L-tryptophan and found that 13.8cA of added radioactivity was recovered from serotonin formed during 18 hrs incubation and the amounts of serotonin formed were depend upon added amount of tryptophan in e99 yolk. It seemed that the serotonin formed from external tryptophan might inhibit the degradation of yolk granule by feedback mechanism, resulting in malformation of chick embryogenesis.

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Recombinant Human Parathyroid Hormone Related Peptide (1-34) Stimulates Osteoclastic Bone Resorption in Both Rodent and Avian Disagsresated Osteoclast Culture (파골세포배야에서 나타난 부갑상선호르몬의 설치류 및 조류 파골세포에 대한 촉진 효과)

  • 양대석;김일찬남궁용이창호
    • The Korean Journal of Zoology
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    • v.37 no.2
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    • pp.255-261
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    • 1994
  • Recombinant human pBrathyriod hormone related peptide (1-341 (rhPTHrP) has been known to stimulate bone resorption in intact bone tissue culture system. Osteoclast has been known as a primary responsible cell for bone resorption. To examine the effect of rhPTHrP on this cell, we employed disaggregated rat osteodast culture. As a result, we found that rhPTHrP sisnificBntly elevates both the number and total area of resorbed pits in this culture. On the other hand, the conflicting results between disagsregated rat osteoc13st culture and Ca2+-deficient hen osteoclast culture system have been a big obstacle for the progress of bone research. To verify the differences between rat 3nd chick osteoclast system, we performed the same experiment using chick embryonic osteoclast. Since the similar results were obtained from the disaggregated chick osteoclast culture, the discrepancy between chick and rat osteoclast culture study seemed to be due to the difference in culture method, rather due to the species-difference.

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Distribution of Doublecortin Immunoreactivities in Developing Chick Retina

  • Kim, Young-Hwa;Sun, Woong
    • Applied Microscopy
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    • v.42 no.3
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    • pp.142-146
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    • 2012
  • Doublecortin (DCX) is a microtuble-associated protein that is required for the migration of immature neuroblasts within the chick and mammalian brain. Although it is generally thought that DCX is expressed only in the neuroblasts, some mature neurons maintain DCX expression; for example, horizontal cells in adult rat retina. In this study, we demonstrate that retinal neural progenitors in the early embryonic stage of the chick also expressed DCX, as do developing ganglion cells and horizontal cells in later stages of development. These findings raise the possibility of a role for DCX in retinal neural progenitors, before they become specialized into neuroblasts in the chick.