• Title, Summary, Keyword: Cell counting method

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음성인식용 DTW PE의 IC화를 위한 ADD 및 ABS 회로의 설계

  • 정광재;문홍진;최규훈;김종교
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.15 no.8
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    • pp.648-658
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    • 1990
  • There are many methods for speed up counting in speech recongition. A multiple processing method is the one way to achieve the aim using systolic array. This arithmetic operation by the array is achieved pipelining skill. And the operation is multiprocessing by processing element(PE) that is incresing counting efficiencies. The DTW PE cell is seperated into three large blocks. "MIN" is the one block for counting accumulated minimum distance, "ADD" block calculated these minimum distances, and "ABS" seeks for the absolut values to the total sum of local distances. We have accomplished circuit design and verification about the "ADD" and "ABS" blocks, and performed total layout '||'&'||' DRC(design rule check) using 3um CMOS N-Well rule base.le check) using 3$\mu$m CMOS N-Well rule base.

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Optical Microscope Image Processing for Automated Cells Counting (세포 자동 계수를 위한 광학현미경 이미지 처리)

  • Cho, Mi-Gyung;Moon, Sang-Jun;Shim, Jae-Sool
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.15 no.11
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    • pp.2493-2499
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    • 2011
  • With growth of nano-bio industry, it is of significant importance to develop an automated system to exploit cell behaviors, including migration, mitosis, apoptosis, shape deformation of individual cells and their interactions among cells in the process of cell growth. In this paper, we proposed preprocessing techniques, a classification method which classifies clusters (overlapping multiple cells) from cells and an automated method which counts the number of cells and clusters in order to analyze 2D or 3D deformations of the cells in the real-time images from microscope in the cell culture. We conducted the 3T3 cell images taken from each thirty-minute interval. It showed the average 99.8% accuracy automatically for separating cells and clusters.

Simple Method for a Cell Count of the Colonial Cyanobacterium, Microcystis sp.

  • Joung, Seung-Hyun;Kim, Choong-Jae;Ahn, Chi-Yong;Jang, Kam-Yong;Boo, Sung-Min;Oh, Hee-Mock
    • Journal of Microbiology
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    • v.44 no.5
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    • pp.562-565
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    • 2006
  • The cell counting of colonial Microcystis spp. is a rather difficult and error-prone proposition, as this genus forms irregularly-shaped and irregularly-sized colonies, which are packed with cells. Thus, in order to facilitate a cell count, four methods of dividing the colonies into single cells were compared, including vortexing, sonication, $TiO_2$ treatment, and boiling. As a result, the boiling method was determined to generate the greatest number of single cells from a colony, and all colonies were found to have divided completely after only 6 min of treatment. Furthermore, no significant cell destruction, which might alter the actual cell density, was detected in conjunction with the boiling method (P=0.158). In order to compute the cell number more simply, the relationship between the colony size and the cell number was determined, via the boiling method. The colony volume, rather than the area or diameter was correlated more closely with the cell number ($r^2=0.727$), thereby suggesting that the cell numbers of colonial Microcystis sp. can also be estimated effectively from their volumes.

Bio-functionalization of the Single Layer Graphene for Detecting the Cancer Cell

  • Oh, Hyung Sik;Park, Wanjun
    • Proceedings of the Korean Vacuum Society Conference
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    • pp.429.1-429.1
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    • 2014
  • We present a method of surface functionalization of a single layer graphene for linking and detecting MDA-MB-231 human breast cancer cell. The methodology is done by utilizing 1-pyrenebutanoic acid and succinimidyl ester for immobiling CD44 antibodies. This work shows that the single layer graphene is an efficient fixing substance to capture the MDA-MB-231 human breast cancer cell, selectively. The immobilization method of the cancer cell on the graphene layer will be an effective cell counting system. Moreover usage of the linking with non-covalent bonding is expected to develope a sensor scheme of electrical cell-detecting diagnosis system.

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Development of Rapid Somatic Cell Counting Method by Using Dye Adding NIR Spectroscopy (색소첨가 NIR을 이용한 우유 체세포수 측정법 개발)

  • Kim, Ke-Sung;Noh, Hae-Won;Lim, Sang-Dong;Choi, Chang-Hyun;Kim, Yong-Joo
    • Food Science of Animal Resources
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    • v.28 no.1
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    • pp.63-68
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    • 2008
  • To develop the somatic cell counting NIR Spectrum method within a range of 400-2500 nm, eosin-Y, methyl red, methylene blue, resazurin and amido black 10B were tested at 0.01% in raw milk. The PLS (Partial Least Square) results are summarized as follows: Correlation coefficients of the calibration model measurements by NIR spectroscopy in raw milk for eosin-Y, methyl red, methylene blue, resazurin and amido black 10B were 0.78, 0.65, 0.63, 0.65, 0.98 and 0.99, respectively. The correlation coefficients of the prediction model measurements by NIR spectroscopy in raw milk for eosin-Y, methyl red, methylene blue, resazurin and amido black 10B were 0.49, 0.21, 0.36, 0.47, 0.95 and 0.98 respectively. Based on these results, amido black 10B was the best additive for the NIRS somatic cell count method.

A New Design of Blood Cell Counter using DSP chip and Optimal Discrimination Method (DSP 칩과 최적분별법을 이용한 새로운 혈구입자 계수기 설계)

  • Kim, G.H.;Kim, J.W.;Kim, K.S.;Hong, W.H.;Kim, S.H.
    • Proceedings of the KOSOMBE Conference
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    • v.1991 no.05
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    • pp.89-93
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    • 1991
  • The purpose of this reserch is to design the blood cell counting instrument which can measure the number of RBC(Red Blood Cell) and WBC(White Blood Cell) including many other blood component. The proposed method uses the electrical impedence method and the new discrimination method wi th DSP chip and software algorithm. The system consist of control unit, blood cell discrimination unit, hemoglobin spectrometer, post detect ion processor unit, and IBM-PC interface unit. In this paper, the discrimination system has been implemented using digital signal processor, which result in the reduction of system hardware and cost. The system is helpful in providing necessary clinical test for screen test and quality control of hematology.

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Studies on the Protoplast Formation of Cellulomonas flavigena and its Observations under Scanning Electron Microscope (Cellulomonas flarigena의 원형질체 형성과 주사전자현미경적 연구)

  • Bae, Moo;Lee, Eun-Ju
    • Microbiology and Biotechnology Letters
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    • v.14 no.2
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    • pp.175-179
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    • 1986
  • In order to develope a protoplast fusion of the genus Cellulomonas having high assimilibility of cellulose, the optimum conditions for the protoplast formation of Cellulomonas flavigena NCIB 12901 was investigated and observed by means of Scanning Electron Microscope. The results suggested that the susceptibility of the cell wall by lysozyme treatment on protoplast formation was considerably depend on the cultural periods of the cells. Cells of C. flavigena at mid exponential phase could more efficiently convert to protoplast cells than those at late exponential phase did. The rate of the protoplast formation was 95%, even though the rate was over 99.9% on counting by indirect method after osmotic shock treatment, when cells of the organism at mid exponential phase were treated with lysozyme (400$\mu\textrm{g}$/$m{\ell}$) for 6 hours and observed by SEM. In the evaluation of protoplast formation of the genus Cellulomonas, direct method of the observation under Scanning Electron Microscope was much more reliable than the counting method of protplasts after osmotic shock treatment. Because defferences between the number of spheroplast and protoplast were not able to be figured out on counting the number of protoplast after osmotic shock treatment.

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Culturing the Uncultured in the Ocean

  • Cho, Jang-Cheon
    • Proceedings of the Microbiological Society of Korea Conference
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    • pp.28-32
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    • 2005
  • Epifluorescence microscopy and direct viable counting methods have shown that only 0.01-0.1% of all the microbial cells from marine environments form colonies on standard agar plates. To culture novel marine microorganisms, high throughput culturing (HTC) techniques were developed to isolate cells in very low nutrient media. This approaches was designed to address microbial metabolic precesses that occur at natural substrate concentrations and cell densities, which are typically about three orders of magnitude less than in common laboratory media. Approximately 5000 cultures of pelagic marine bacteria were examined over the course of 3 years. Up to 14% of cells from coastal seawater were cultured using this method, a number that is 1400 to 140-fold higher than obtained by traditional microbiological culturing techniques. Among the cultured organisms are many unique phylogenetic lineages that have been named as new phyla (7), orders (2, 5, 12), families (3), and genera (1, 4, 6). Over 90% of the cells recovered by this method do not replicate in standard agar plating, the most common method of microbial cell cultivation.

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A STUDY OF THE CYTOTOXICITY OF ROOT CANAL SEALER IN VITRO (생체외 실험을 이용한 근관충전용 Sealer의 세포독성에 관한 연구)

  • Lee, Sang-Tag;Lee, Chung-Sik
    • Restorative Dentistry and Endodontics
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    • v.16 no.2
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    • pp.62-84
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    • 1991
  • The purpose of this study was to evaluate the cytotoxicity of four root canal sealers(Tubliseal, AH26, Apatite Root Canal Sealer I, Apatite Root Canal Sealer II) in Vitro. The root canal sealers were mixed and filled in molds which were $14{\times}1.25mm$ in diameter, in height to use for cell counting and agar overlary method, and $7{\times}1.25mm$ for millipore filter method and set for 7 days to use for experiment. Silicone and copper plate were used for negative and positive control respectively. Using the culture of L929 fibroblast, total cell number and vital cell number were counted and the ratio of vital cell number to total cell number was calculated on 2 nd, 4 th, 6 th experimental day, and the change of cell membrane permeability was tested by agar overlay method, and the succinate dehydrogenase activity was tested by millipore filter method. The obtained results were as follows. 1. In ail experimental groups, the mitotic activity of fibroblast was reduced when compared with that of negative control group, so ail experimental groups showed cytotoxicity. Apatite Root Canal Sealer I group exhibited mild cytotoxicity, and Tubliseal, AH26, Apatite Root Cenal Sealer II groups exhibited severe cytotoxicity. 2. In the test of the change of cell membrane permeability by agar overlay method, all experimental groups showed cytotoxicity. AH26 group exhibited mild cytotoxicity, and Apatite Root Canal Sealer I group exhibited moderate cytotoxicity, and Tubliseal and Apatite Root Canal Sealer II group exhibited severe cytotoxicity. 3. In the test of SDH activity by millipore filter method, there was no cytotoxicity in Apatite Root Canal Sealer I and Apatite Root Canal Sealer II group, but Tubliseal and AH26 group showed mild cytotoxicity.

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Acceleration of Cell Proliferation and Gene Expression in Human Chondrosarcoma Cells Stimulated by Strong Pulse Magnetic Field

  • Shin, Sung Chul;Chung, Eui Ryong;Hwang, Do Guwn
    • Journal of Magnetics
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    • v.18 no.1
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    • pp.14-20
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    • 2013
  • For the treatment of osteoarthritis, pulsed electromagnetic field stimulus has been suggested as a useful therapeutic method in rehabilitative medicine. Most studies have been performed under low-frequency and low-energy to find out biological properties for stimulating chondrocyte with pulsed magnetic field. In this study, the effect of strong pulse magnetic field on the human chondrosarcoma cells (SW-1353) has been investigated by means of cell counting, morphologies, and gene expression of cartilage extracellular matrix genes. The SW-1353 cells were exposed under the field intensities of 270, 100, 55, 36, and 26 mTesla during 6 hours a day in 5 consecutive days. The pulse magnetic field with an LRC oscillating signal has the pulse width of 0.126 msec and stimulation period of 1 sec. For the 270 and 100 mTesla stimulation, the cell proliferation significantly increased in 21-24% as compared with the non-stimulated cells. Gene expression of cartilage extracellular matrix genes (ACAN, COMP and COL2A1) was assayed by quantitative real time-PCR method. The ACAN gene expression showed a significant brightness, which means the increase on gene expression, compared with the non-stimulated cells. Our results suggest that the strong pulse magnetic field stimulation can be utilized to accelerate cell proliferation and gene expression on human chondrosarcoma cells.