• Title/Summary/Keyword: Capsicum annuum

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Effects of some edible plants on changing chromosomal morphology, and apoptosis of MCF-7 cell (식용식물들이 MCF-7 cell의 chromosome형태에 미치는 영향)

  • 정용자;곽수영
    • Journal of Life Science
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    • v.12 no.3
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    • pp.340-348
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    • 2002
  • Equisetum arvense L. and Lactuca dentata Makino. var. Flaviflora Makino. of samples relatively showed anticancer effects on MCF-7 mammary gland adenocarcinoma cell. The most active plant among the samples was Capsicum annuum L. var angulosum Mill. We studied that MCF-7 cells were changing chromosomal morphology and apoptosis on these samples. Capsicum annuum L. var. angulosum Mill. of samples relatively showed good anticancer effects. The cells became vague after 2 days and then destroyed. The supernatant of the cells including medium was measured by UV absorbance. The results showed that Capsicum annuum L. var. angulosum Mill also exerted high level. We also used electrophoresis in order to observe apoptic characterization of DNA fragmentation. The cells treated with Capsicum annuum L. var. angulosum Mill showed the apoptotic characterization. The chromosome of the cells were observed on those samples. The cells treated with Capsicum annuum L. var. angulosum Mill among them were shown the fastest changes. The cells were aggregated and destroyed by treatment with some edible plants. Especially, the case of Capsicum annuum L. var. angulosum Mill, it led MCF-7 cell to apoptosis faster than others. And we can observe chromosomal changes and dispersion by PI staining. These results showed that each sample exerted anticancer effects on MCF-7 cells. Especially Capsicum annuum L. var. angulosum Miff exerted significant anticancer effects.

Comparison Study of Three Varieties of Red Peppers in Terms of Total Polyphenol, Total Flavonoid Contents, and Antioxidant Activities (품종별 고추(꽃고추, 홍고추 및 홍피망)의 총폴리페놀, 총플라보노이드 함량 및 항산화 활성 비교)

  • Byun, Eui-Baek;Park, Woo-Young;Ahn, Dong-Hyun;Yoo, Yung-Choon;Park, Chulhwan;Park, Won-Jong;Jang, Beom-Su;Byun, Eui-Hong;Sung, Nak-Yun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.5
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    • pp.765-770
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    • 2016
  • This study compared the extracts of three varieties of red peppers in terms of their total polyphenol and flavonoid contents as well as antioxidant activities. In this study, two edible red peppers (Capsicum annuum var. angulosum and Capsicum annuum L.) and one flowering grass red pepper (Capsicum annuum var. abbreviatum), were individually extracted by using ethanol. Among three varieties of red peppers, the highest levels of total polyphenols and total flavonoids were observed in Capsicum annuum var. abbreviatum extract. Similarly, antioxidant activities resulted in ${\alpha},{\alpha}$-diphenyl-picrylhydrazyl, 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities, and reducing power significantly increased in extract of Capsicum annuum var. abbreviatum. Therefore, our results strongly suggest that Capsicum annuum var. abbreviatum can be used as a new natural antioxidant source.

Anti-Oxidative and Anti-Proliferative Effect of 70% Ethanol Extracts from Green Pepper (Capsicum annuum L. cv. DangZo) (당조고추 70% 에탄올추출물의 항산화 및 항암활성측정)

  • Lee, Youn Ri
    • The Korean Journal of Food And Nutrition
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    • v.30 no.5
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    • pp.1127-1131
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    • 2017
  • To evaluate the anti-oxidant and anti-tumor potential of the green pepper (Capsicum annuum L. cv. DangZo), total polyphenol content, radical scavenging activities and anti-tumor properties were measured. The total polyphenol content of the 70% ethanol extracts from green pepper (Capsicum annuum L. cv. DangZo) was 30.29 mg gallic acid equivalent/g extract. The DPPH radical and hydroxyl radical scavenging activities of 70% ethanol extracts of green pepper (Capsicum annuum L. cv. DangZo) were documented at 2.87 and 10.55, respectively. For ${\alpha}$-glucosidase and ${\alpha}$-amylase inhibitory activity, 70% ethanol extracts of green pepper (Capsicum annuum L. cv. DangZo) were documented at 35.67% and 58.41% respectively. The green pepper (Capsicum annuum L. cv. DangZo) demonstrated greater capability in terms of anti-neoplastic activity vis-a-vis colon cancer cell lines when compared to other cancer cell lines.s. er (Capsicum annuum L. cv. DangZo) higher activities of anticancer activities on colon cancer cell lines compared to other cancer cell lines.

The Ozone Stress Transcriptome of Pepper (Capsicum annuum L.)

  • Lee, Sanghyeob;Yun, Sung-Chul
    • Molecules and Cells
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    • v.21 no.2
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    • pp.197-205
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    • 2006
  • We used cDNA microarrays to monitor the transcriptome of ozone stress-regulated genes (ORGs) in two pepper cultivars [Capsicum annuum cv. Dabotop (ozone-sensitive) and Capsicum annuum cv. Buchon (ozone-tolerant)]. Ozone stress up- or down-regulated 180 genes more than three-fold. Transcripts of 84 of these ORGs increased, transcripts of 88 others diminished, and those of eight either accumulated or diminished at different time points in the two cultivars or changed in only one of the cultivars. 67% (120) of the ORGs were regulated differently in ozone-sensitive and ozone-tolerant peppers, most being specifically up-regulated in the ozone-sensitive cultivar. Many were also represented in the plant defense transcriptome against non-host pathogen infection, and some in the transcriptomes for cold, drought, and salinity stresses.

Apoptotic Effects of Some Plants on MCF-7 Mammary Gland Adenocarcinoma Cells (수종식물의 MCF-7 세포에 대한 세포사 및 항ㆍ증식효과)

  • 정용자
    • Journal of Life Science
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    • v.14 no.1
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    • pp.61-66
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    • 2004
  • Many studies have been widely carried out to find out new compound having anti-cancer activity from animals and plants. Some plants have been reported to have anti-lancer effects. However, the anti-cancer effect of edible plants were seldomly evaluated. Therefore we investigated the anticancer effects of edible plants (10 samples) easily available around us by measuring number of survival cancer cells after treatment with direct cell counting and MTT analysis, and by examining the morphological change under the electromicroscope. Of the 10 samples tested, Equisetum arvense L., Lactuca dentata Mokino. var. faviflora Makino. showed moderate anti-cancer effects even at the concentration of 10 $\mul/ml$ against MCF-7 adenocarcinoma cell line. Of them, Capsicum annuum L. had most potent anti-cancer activity against MCF-7 adenocarcinoma cell line showing proliferation inhibited, morphological change and apoptosis at the concentration of 2 $\mul/ml$.

Development of Macaronè with Korean Red Peppers (Capsicum annuum L.) and GABA rice and Evaluation of Physiological Characteristics (고추와 가바쌀을 첨가한 기능성 마카롱 개발에 관한 연구)

  • Yoo, Kyung-Mi
    • The Korean Journal of Food And Nutrition
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    • v.28 no.3
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    • pp.351-357
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    • 2015
  • The aims of this study were to develop $macaron{\grave{e}}$ with Korean red pepper (Capsicum annuum L.) and GABA (${\gamma}$-aminobutyric acid) rice powder and analyze their physico-chemical and physiological characteristics. Korean red pepper (Capsicum annuum L.) was added to $macaron{\grave{e}}$ at a weight percentage of 0, 2.5 and 4.5%. Color values (L-value, redness, and yellowness), total sugar content, total phenolics, total carotenoids, ABTS radical scavenging activity, textures, and sensory characteristics with varying various Korean red pepper were measured. As the ratio of the powders in the $macaron{\grave{e}}s$ increased, total phenolic content, total carotenoid content, and radical scavenging activity increased. There were significant differences in total phenolic and total carotenoid content of $macaron{\grave{e}}s$ (p<0.05). Sensory evaluation indicated significant differences (p<0.05) in color, sweetness, texture, and overall acceptability depending on the addition of Korean red pepper. KR30 $macaron{\grave{e}}$, showed higher overall acceptability, as compared to other $macaron{\grave{e}}$.

Stem Rot of Capsicum annuum Caused by Sclerotium relfsii in Korea (Sclerotium rolfsii에 의한 고추 흰비단병 발생)

  • Kwon, Jin-Hyeuk;Park, Chang-Seuk
    • Research in Plant Disease
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    • v.10 no.1
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    • pp.21-24
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    • 2004
  • A destructive stem rot of pepper (Capsicum annuum) was found from the often field sporadically in Jingyemyon, Hadong-gun in July 2002 and vinyl houses in Moonsan-eup, Jinju City in October 2003. The same fungus also caused collar and crown rot and systemic wilt or blight of whole plant. White mycelium spread over stems of infected plants and sclerotia formed on the old lesions and near the soil surface. The fungus showed maximum mycelial growth around 3$0^{\circ}C$. The mycelial color is white and width of hyphae ranges 3.6∼10.2 ${\mu}{\textrm}{m}$, and formed clamp connection. Numerous sclerotia were farmed in artificial media such as PDA at 3$0^{\circ}C$. The shape of sclerotia were sphere and 1.0∼2.1 mm in diameter, The fungus was isolated repeatedly from the infected tissues and the pathogenecity of fungus to pepper (Capsicum annuum) was confirmed, and identified as Sclerotium rolfsii. This is the first report on the stem rot of pepper (Capsicum annuum) caused by Sclerotium rolfsii in Korea.

Optimized Protocols for Efficient Plant Regeneration and Gene Transfer in Pepper (Capsicum annuum L.)

  • Mihalka, Virag;Fari, Miklos;Szasz, Attila;Balazs, Ervin;Nagy, Istvan
    • Journal of Plant Biotechnology
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    • v.2 no.3
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    • pp.143-149
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    • 2000
  • An Efficient in vitro regeneration system and an optimized Agrobacterium mediated transformation protocol are described, based on the use of young seedling cotyledons of Capsicum annuum L. Optimal regeneration efficiency can be obtained by cultivating cotyledon explants on media containing 4 mg/L benzyladenine and 0.1 mg/L indolacetic acid. The effect of antibiotics used to eliminate Agrobacteria, as well as the toxic level of some generally used selection agents (kanamycin, geneticin, hygromycin, phosphinotricin and methotrexate) in regenerating pepper tissues were determined. To enable the comparison of different selection markers in identical vector background, a set of binary vectors containing the marker genes for NPTII, HPT, DHFR and BAR respectively, as well as the CaMV 35S promoter/enhancer-GUS chimaeric gene was constructed and introduced into four different Agrobacterium host strains.

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Molecular and Morphological Characterization of Endophytic Heterobasidion araucariae from Roots of Capsicum annuum L. in Korea

  • Paul, Narayan Chandra;Deng, Jian Xin;Shin, Kyu-Seop;Yu, Seung-Hun
    • Mycobiology
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    • v.40 no.2
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    • pp.85-90
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    • 2012
  • A species of Heterobasidion was encountered during a diversity study of endophytic fungi from healthy root tissues of chili pepper (Capsicum annuum L.) in Korea. The fungal species (CNU081069) was identified as Heterobasidion araucariae based on phylogenetic analyses of the internal transcribed spacer and translation elongation factor gene sequences. Morphological descriptions of the endophytic isolate matched well with the previous references and supported the molecular identification. The fungus Heterobasidion araucariae CNU081069 is new to Korea.