• Title, Summary, Keyword: Candidemia

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Late Onset Candida albicans Spondylodiscitis Following Candidemia: A Case Report

  • Lee, Jung Hwan;Chough, Chung Kee;Choi, Su Mi
    • Journal of Mycology and Infection
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    • v.22 no.3
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    • pp.117-121
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    • 2017
  • Candida albicans infections of the spine are relatively uncommon in spite of the increasing frequency of predisposing factors. Moreover, late onset spondylodiscitis after bloodstream candidiasis is extremely rare. A 66-year-old woman to have been underwent chemotherapy was diagnosed with candidemia. Antifungal agent was administrated until two weeks after no detection of fungus in the blood culture. The chemotherapy was continued. But, she was hospitalized due to abdominal pain and diarrhea. Pseudomembranous colitis was diagnosed. After metronidazole administration, she was improved and discharged. However, she revisited because of back pain and fever. Spondylitis and discitis on the 10th~11th thoracic spine was shown in magnetic resonance images. Open curettage and spinal stabilization was performed. C. albicans was identified. Antifungal agent was administrated and the patient improved well postoperatively. We present a rare case of late onset Candida spondylodiscitis after candidemia with review of the literatures.

Molecular Epidemiological Analysis of Bloodstream Isolates of Candida albicans from a University Hospital over a Five-Year Period

  • Shin Jong Hee;Og Yu Gyung;Cho Duck;Kee Seung Jung;Shin Myung Geun;Suh Soon Pal;Ryang Dong Wook
    • Journal of Microbiology
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    • v.43 no.6
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    • pp.546-554
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    • 2005
  • We assessed the genetic relations and epidemiological links among bloodstream isolates of Candida albicans, which were obtained from a university hospital over a period of five years. The 54 bloodstream isolates from the 38 patients yielded 14 different karyotypes, 29 different patterns after digestion with SfiI (REAG-S), and 31 different patterns after digestion with BssHII (REAG-B) when analyzed using three different pulsed-field gel electrophoresis (PFGE) typing methods. In 11 patients with serial blood stream isolates, all strains from each patient had the same PFGE pattern. The dendrograms for all of the strains revealed that the distribution of similarity values ranged from 0.70 to 1.0 in the REAG-S patterns, and from 0.35 to 1.0 in the REAG-B patterns. Overall, the combination of the three different PFGE methods identified 31 distinct types, reflecting the results obtained using the REAG-B alone different. different Five PFGE types were shared among 22 isolates from 12 patients. These types of strains were more frequently associated with central venous catheter-related fungemia than the other 26 type strains $(92\%\;versus\;31\%;\;P<0.005)$. Of five PFGE types, four isolates were determined to be epidemiologically related: each of these types was primarily from two or three patients who had been hospitalized concurrently within the same intensive care unit. Our results suggest that the REAG-B constitutes perhaps the most useful PFGE method for investigating C. albicans candidemia and also shows that a relatively high proportion of C. albicans candidemia may be associated with exogenous acquisition of clonal strains.

High-dose caspofungin salvage in a very-low-birth-weight infant with refractory candidemia (극소 저체중 출생아의 난치성 칸디다혈증에서 고용량 Caspofungin 구제요법)

  • Seo, Eun Sun;Park, Geun Hwa;Kim, Sung Mi;Jung, Hye An;Kim, Byoung Kuk
    • Korean Journal of Pediatrics
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    • v.53 no.2
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    • pp.239-243
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    • 2010
  • Candidiasis is one of the most common causes of late-onset infection among very-low-birth-weight infants (VLBW) in most neonatal intensive care units and is associated with significant morbidity and mortality. Standard therapy consists of the administration of amphotericin B, amphotericin B complex, and fluconazole. In many cases, candidiasis is not easily eradicated, despite the administration of these drugs. We report our experience of the addition of high-dose caspofungin to the conventional antifungal drugs in a VLBW infant with refractory candidemia.

Candida non albicans with a High Amphotericin B Resistance Pattern Causing Candidemia among Cancer Patients

  • Kalantar, Enayatollah;Assadi, Mojan;Pormazaheri, Helen;Hatami, Shiva;Barari, Maryam Agha;Asgari, Esfandiar;Mahmoudi, Elaheh;Kabir, Kourosh;Marashi, Seyed Mahmoud Amin
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.24
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    • pp.10933-10935
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    • 2015
  • Background: Many scientists have reported Candida species to be of great concern because of the high frequency that they colonize and infect human hosts, particularly cancer patients. Moreover, in the last decades Candida species have developed resistance to many antifungal agents. Based on this, we aimed to identify and determine the prevalence of Candida spp from blood culture bottles among cancer patients and their antifungal resistance pattern. Materials and Methods: From the blood culture bottles isolation and identification of the Candida spp were performed by conventional microbiological techniques. The in vitro antibiotic resistance pattern of the isolates was determined by CLSI guidelines. Genomic DNA was isolated and amplified. Each gene was separated by agar gel electrophoresis. Results: Identification of Candida spp was based on the presence of yeast cells in direct examination, culture and DNA extraction. Of the 68 blood samples collected during the study period (April 2013 to October 2013), five (7.35%) were positive for the presence of Candida spp, 2 (40%) of which were identified as Candida albicans and 3 (60%) were Candida non-albicans. Conclusions: High resistance to amphotricin B was observed among all the Candida non-albicans isolates. Regular investigations into antifungal resistance will help us to get an updated knowledge about their antibiotic resistance pattern which may help the physician in selecting the antibiotics for empirical therapy.

Infected Thoracic Aortic Aneurysm Concurrent with Endophthalmitis by Candida albicans: a Case Report (Candida albicans에 의한 칸디다 안구내염에 병발한 감염성 흉부 대동맥류 1예)

  • Kwon, Hyun Hee
    • Journal of Mycology and Infection
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    • v.22 no.4
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    • pp.172-177
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    • 2017
  • Candida species cause various invasive fungal diseases, including candidemia, endocarditis, endophthalmitis, peritonitis, osteomyelitis and arthritis, but infected (mycotic) aortic aneurysms caused by Candida species are very rare. So, we report a case of infected thoracic aortic aneurysm concurrent with endophthalmitis by Candida albicans in a 42-year-old man. The patient initially was diagnosed with candidial endophthalmitis and hospitalized for vitrectomy. On admission, he had chest CT taken and infected thoracic aortic aneurysm was detected. He treated with antifungal agent and resection and patch repair of aortic aneurysm. Two months later, a new aneurysm on the patch repair site was detected and thoracic endovascular aortic repair (TEVAR) was performed. After TEVAR and long-term antifungal therapy, his infected aortic aneurysm has been successfully treated.

Purification of Enolase from Candida albicans KNIH10 Isolated in Korea and Application of Immunological Diagnosis (Candida albicans KNIH10으로부터 Enolase의 분리 및 면역진단의 응용)

  • Park, Yong-Chjun;Yoo, Jae-Il;Lee, Yeong-Seon;Shin, Jong-Hee;Kim, Bong-Su
    • The Journal of the Korean Society for Microbiology
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    • v.35 no.2
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    • pp.141-147
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    • 2000
  • We purified enolase from Candida albicans KNIH10 strain which was isolated from a clinical specimen in Korea. The purified enolase was used to detect anti-Candida antibodies in sera of patients with invasive candidiasis. For purification of enolase from the crude extract prepared by French pressure at 20,000 PSI, the fast performance liquid chromatography (FPLC) using DEAE-sepharose column was used. The elutes at $0.3{\sim}0.4\;M$ NaCl in FPLC was purified with homogenity in SDS-PAGE and its enzymatic activity was confirmed in sera of invasive candidiasis with candidemia patient by immunoblotting. The purified enolase indicated no signal (100% specificity) in 40 normal human sera and 75% (6/8) sensitivity in sera of candidemic patients with suspicious invasive candidiasis by immunoblotting.

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Differentiation of Candida albicans and Candida dubliniensis using Latex Agglutination Test (라텍스 응집 검사를 이용한 Candida albicans와 Candida dubliniensis의 감별)

  • Kim, Tae-Hyoung;Lee, Mi-Kyung
    • Journal of Mycology and Infection
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    • v.22 no.1
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    • pp.15-20
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    • 2017
  • Background: Candida dubliniensis is phenotypically similar to Candida albicans that may be underdiagnosed in clinical laboratory. In 2010, C. dubliniensis was first recovered from blood of a candidemia patient in Seoul, Korea. Also, a simple commercial latex agglutination (LA) test is available. Objective: The aim of the present study was to investigate the prevalence of C. dubliniensis among isolates in our stocks during 2-years period (2010-2011) and to evaluate the ability of LA test (Bichro-Dubli $Fumouze^{(R)}$) for the differentiation of C. albicans and C. dubliniensis. Methods: A total 509 isolates including 504 C. albicans and 5 C. dubliniensis were examined for LA test, the presence of "spiking" on blood agar plate, and the germ tube test. Also all isolates were tested using the VITEK 2 ID-YST system. Results: No C. dubliniensis was found in 504 isolates of initially identified as C. albicans. The LA test was positive only in 5 clinical isolates and 2 type strains of C. dubliniensis. Conclusions: The data show that the prevalence of C. dubliniensis in Korea is still expected to be extremely low and LA test is very rapid, simple, and reliable tool for the differentiation of C. albicans and C. dubliniensis.

Genetic Variations of Candida glabrata Clinical Isolates from Korea using Multi-locus Sequence Typing (Multi-locus sequence typing을 이용한 한국에서 분리한 Candida glabrata 임상균주의 유전자 유형 분석)

  • Kang, Min Ji;Lee, Kyung Eun;Jin, Hyunwoo
    • Journal of Life Science
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    • v.30 no.2
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    • pp.122-128
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    • 2020
  • Although Candida albicans is the major fungal pathogen of candidemia, severe infections by non-albicans Candida (NAC) spp. have been increasing in recent years. Among NAC spp., C. glabrata has emerged as the second most common pathogen. However, few studies have been conducted to investigate its structure, epidemiology, and basic biology. In the present study, multi-locus sequence typing (MLST) was performed with a total of 102 C. glabrata clinical isolates that were isolated from various types of clinical specimen. For MLST, six housekeeping genes-FKS, LEU2, NMT1, TRP1, UGP1, and URA3-were amplified and sequenced. The results were analyzed using the C. glabrata database. Out of a total of 3,345 base-pair DNA sequences, 49 variable nucleotide sites were found, and the results showed that 12 different sequence types (ST) were identified from the 102 clinical isolates. The data also demonstrated that the undetermined ST1 was the most predominant ST in Korea. Further, seven undetermined STs (UST) containing UST2-8 were classified at specific loci. The data from this study may provide a fundamental database for further studies on C. glabrata, including its epidemiology and evolution. The data may also contribute to the development of novel antifungal agents and diagnostic tests.