• Title, Summary, Keyword: CSL

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생균제로서 Pichia anamala의 pilot scale배양조건 및 동결건조의 최적화

  • Yun, Ji-Yong;Lee, Yong-Ho;Choe, Yu-Jin;Seong, Chang-Geun;Han, Gyu-Beom
    • 한국생물공학회:학술대회논문집
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    • pp.157-160
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    • 2002
  • Probiotics can form profitab1e microorganisms in human or animal internal organs. When it is fed at domastic animals, can bring the rise of weight and prevention of disease. Pichia anonwla by new probiotics have antibiotic effect and oder reducing effect. we researched about optimal condition of pilot scale fermentation and freezed dry of pichia anonwla.

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성대폴립 환자를 대상으로 한 GRBAS 척도와 MDVP 측정치 간의 상관관계 연구

  • 표화영;최성희;임성은;심현섭;최홍식;김광문
    • Proceedings of the KSLP Conference
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    • pp.180-180
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    • 1999
  • 배경 : 음성평가시 그 음질을 파악하기 위한 검사 중 주관적 검사로서 많이 쓰이는 것은 GRBAS 척도에 의한 청인지적 4단계 평정이고, 객관적 검사로서 널리 쓰이고 있는 검사 도구는 CSL 중 MDVP이다. 이중 GRBAS 검사는 평가자의 고도의 숙련도를 요구하며, MDVP에 의한 음성평가는, 나타난 결과에 대한 정확한 해석을 요구한다. 목적 : 이에, 1차적으로 음성과용 환자를 대상으로 하여, GRBAS 척도에 의한 청인지적 음성평가 결과와 CSL 중 MDVP에 의한 객관적 음성평가 결과에 대한 상관성을 비교, 분석해 보고, 이를 통하여 청인지적 음성평가에 숙련되지 못한 평가자가 객관적 검사도구를 통하여 음성평가를 하고자 할 때 주목해야 할 변인들에 관하여 알아보고자 한다. (중략)

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Realization of Two-bit Operation by Bulk-biased Programming Technique in SONOS NOR Array with Common Source Lines

  • An, Ho-Myoung;Seo, Kwang-Yell;Kim, Joo-Yeon;Kim, Byung-Cheul
    • Transactions on Electrical and Electronic Materials
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    • v.7 no.4
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    • pp.180-183
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    • 2006
  • We report for the first time two-bit operational characteristics of a high-density NOR-type polysilicon-oxide-nitride-oxide-silicon (SONOS) array with common source line (CSL). An undesired disturbance, especially drain disturbance, in the NOR array with CSL comes from the two-bit-per-cell operation. To solve this problem, we propose an efficient bulk-biased programming technique. In this technique, a bulk bias is additionally applied to the substrate of memory cell for decreasing the electric field between nitride layer and drain region. The proposed programming technique shows free of drain disturbance characteristics. As a result, we have accomplished reliable two-bit SONOS array by employing the proposed programming technique.

Logging for a Stone Column Using Crosshole Seismic Testing (크로스홀 탄성파 시험을 이용한 쇄석다짐말뚝의 검측)

  • Kim, Hak-Sung;Park, Chul-Soo;Lee, Tae-Hee;Mok, Young-Jin
    • Proceedings of the Korean Geotechical Society Conference
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    • pp.84-90
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    • 2009
  • An integrity testing for stone columns was attempted using crosshole S-wave logging. The method is conceptionally quite similar to the crosshole sonic logging (CSL) for drilled piers. The critical difference in the logging is the use of s-wave rather than p-wave, which is used in CSL, because s-wave is the only wave sensing the stiffness of slower unbounded materials than water. An electro-mechanical source, which can generate reversed S-wave signals, was utilized in the logging. The stone column was delineated from the S-wave travel times across the stone column, and taking S-wave velocities of the crushed stone and surrounding soil into account. The volume calculated from the diametrical variance delineated is very close to the actual quantity of the stone filled.

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MULTI-SCALE MODELING AND ANALYSIS OF CONVECTIVE BOILING: TOWARDS THE PREDICTION OF CHF IN ROD BUNDLES

  • Niceno, B.;Sato, Y.;Badillo, A.;Andreani, M.
    • Nuclear Engineering and Technology
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    • v.42 no.6
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    • pp.620-635
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    • 2010
  • In this paper we describe current activities on the project Multi-Scale Modeling and Analysis of convective boiling (MSMA), conducted jointly by the Paul Scherrer Institute (PSI) and the Swiss Nuclear Utilities (Swissnuclear). The long-term aim of the MSMA project is to formulate improved closure laws for Computational Fluid Dynamics (CFD) simulations for prediction of convective boiling and eventually of the Critical Heat Flux (CHF). As boiling is controlled by the competition of numerous phenomena at various length and time scales, a multi-scale approach is employed to tackle the problem at different scales. In the MSMA project, the scales on which we focus range from the CFD scale (macro-scale), bubble size scale (meso-scale), liquid micro-layer and triple interline scale (micro-scale), and molecular scale (nano-scale). The current focus of the project is on micro- and meso-scales modeling. The numerical framework comprises a highly efficient, parallel DNS solver, the PSI-BOIL code. The code has incorporated an Immersed Boundary Method (IBM) to tackle complex geometries. For simulation of meso-scales (bubbles), we use the Constrained Interpolation Profile method: Conservative Semi-Lagrangian $2^{nd}$ order (CIP-CSL2). The phase change is described either by applying conventional jump conditions at the interface, or by using the Phase Field (PF) approach. In this work, we present selected results for flows in complex geometry using the IBM, selected bubbly flow simulations using the CIP-CSL2 method and results for phase change using the PF approach. In the subsequent stage of the project, the importance of effects of nano-scale processes on the global boiling heat transfer will be evaluated. To validate the models, more experimental information will be needed in the future, so it is expected that the MSMA project will become the seed for a long-term, combined theoretical and experimental program.

Factorial design에 의한 Acetobacter xylinum KJ1의 Bacterial cellulose 생산조건의 최적화

  • Lee, Ji-Eun;Jeong, Sang-Gi;Lee, Yong-Un;Jeong, Seon-Yong;Kim, Seong-Jun
    • 한국생물공학회:학술대회논문집
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    • pp.131-135
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    • 2002
  • Acetobacter xylinum KJ1 efficiently producing bacterial cellulose(BC) in shaking culture was isolated from a rotten grape. The strain was used to investigate optimum operating conditions for increasing BC production and factorial design model was employed for the optimization. The results of experiments were statistically analyzed by SAS program. Reciprocal effects of each factors(carbon source concentration, shaking speeds(rpm), oxygen pressure, and CSL concentration) and culture condition of BC production were examined by getting regression equation of the dependent variable. Comparisons between experimental results and predicted results about BC concentration were done in total 24 experiments by combination of each factors using SAS program, and the correlation coefficients of BC concentration and BC yield were 0.91 and 0.81, respectively. The agitated cultures were performed in various operation conditions of factors which affected considerably to BC production in jar fermentor. The results showed that BC concentration was 11.67g/ L in 80 hours cultivation under the condition of carbon source concentration shaking speeds(rpm) : oxygen pressure: CSL concentration = 4% : 460rpm : 0.28 : 6%. On the other hand BC yield was 0.42g/g in 80 hours cultivation under the condition of carbon source concentration shaking speeds(rpm) : oxygen pressure: CSL concentration = 4% : 564rpm : 0.21 : 2%. The BC production could be enhanced up to more than 65.3% by factorial design. The result of a verifying experiment under the optimal conditions determined by the factorial design to the BC production showed that the model was appropriate by obtaining BC concentration of 11.02g/L in the optimum condition

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Characterization of Ribose-5-Phosphate Isomerase B from Newly Isolated Strain Ochrobactrum sp. CSL1 Producing ʟ-Rhamnulose from ʟ-Rhamnose

  • Shen, Min;Ju, Xin;Xu, Xinqi;Yao, Xuemei;Li, Liangzhi;Chen, Jiajia;Hu, Cuiying;Fu, Jiaolong;Yan, Lishi
    • Journal of Microbiology and Biotechnology
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    • v.28 no.7
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    • pp.1122-1132
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    • 2018
  • In this study, we attempted to find new and efficient microbial enzymes for producing rare sugars. A ribose-5-phosphate isomerase B (OsRpiB) was cloned, overexpressed, and preliminarily purified successfully from a newly screened Ochrobactrum sp. CSL1, which could catalyze the isomerization reaction of rare sugars. A study of its substrate specificity showed that the cloned isomerase (OsRpiB) could effectively catalyze the conversion of $\text\tiny{L}$-rhamnose to $\text\tiny{L}$-rhamnulose, which was unconventional for RpiB. The optimal reaction conditions ($50^{\circ}C$, pH 8.0, and 1 mM $Ca^{2+}$) were obtained to maximize the potential of OsRpiB in preparing $\text\tiny{L}$-rhamnulose. The catalytic properties of OsRpiB, including $K_m$, $k_{cat}$, and catalytic efficiency ($k_{cat}/K_m$), were determined as 43.47 mM, $129.4sec^{-1}$, and 2.98 mM/sec. The highest conversion rate of $\text\tiny{L}$-rhamnose under the optimized conditions by OsRpiB could reach 26% after 4.5 h. To the best of our knowledge, this is the first successful attempt of the novel biotransformation of $\text\tiny{L}$-rhamnose to $\text\tiny{L}$-rhamnulose by OsRpiB biocatalysis.

Osmoprotective Effect of Glycine Betaine on Foreign Protein Production in Hyperosmotic Recombinant Chinese Hamster Ovary Cell Cultures Differs among Cell Lines

  • Ryu, Jun-Su;Kim, Tae-Gyeong;Jeong, Ju-Yeong;Lee, Gyun-Min
    • 한국생물공학회:학술대회논문집
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    • pp.313-316
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    • 2000
  • When 3 recombinant Chinese hamster ovary (rCHO) cell lines, CHO/dhfr-B-22-4, $CS13-1.00^{\ast}$ and $CSl3-0.02^{\ast}$, were cultivated in hyperosmolar media resulting from NaCl addition, their specific foreign protein productivity increased with medium osmolality. Glycine betaine was found to have a strong osmoprotective effect on all 3 rCHO cell lines. Inclusion of 15 mM glycine betaine in hyperosmolar medim enabled rCHO cell lines to grow at 557-573 mOsm/kg where they could not grow in the absence of glycine betaine. However, effect of glycine betaine inclusion in hyperomolar medium on foreign protein production differed among rCHO cell lines. CHO/dhfr-B22-4 cells retained enhanced specific human thrombopoietin (hTPO) productivity in the presence of glycine betaine, and thereby, the maximum hTPO titer obtained at 573 mOsm/kg was increased by 72% over that obtained in the control culture with physiological osmolality (292 mOsm/kg). On the other hand, enhanced specific antibody productivity of $CSl3-1.00^{\ast}$ and $CSl3-0.02^{\ast}$ at elevated osmolality decreased significantly in the presence of glycine betaine at a cost of the recovery of cell growth. As a result, the maximum antibody titer at 557 mOsm/kg was similar to that obtained in the control culture with physiological osmolality. Taken together, efficacy of the simultanous use of hyperosmotic pressure and glycine betaine as a means to improve foreign protein production was variable among different rCHO cell lines.

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Protective effects of Bacillus subtilis against Salmonella infection in the microbiome of Hy-Line Brown layers

  • Oh, Ju Kyoung;Pajarillo, Edward Alain B.;Chae, Jong Pyo;Kim, In Ho;Kang, Dae-Kyung
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.9
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    • pp.1332-1339
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    • 2017
  • Objective: This study investigated the effects of Bacillus subtilis CSL2 (B. subtilis CSL2) administration before Salmonella challenge on the fecal microbiota and microbial functionality of Hy-line Brown (HLB) laying hens. Methods: Fecal samples were collected from control (CON), Salmonella-infected (SAL) and Salmonella-infected, probiotic-treated (PRO) groups before and after Salmonella challenge for microbiome analysis using 16S rRNA gene pyrosequencing. Results: Infection with Salmonella led to decreased microbial diversity in hen feces; diversity was recovered with Bacillus administration. In addition, Salmonella infection triggered significant alterations in the composition of the fecal microbiota. The abundance of the phylum Firmicutes decreased while that of Proteobacteria, which includes a wide variety of pathogens, increased significantly. Bacillus administration resulted in normal levels of abundance of Firmicutes and Proteobacteria. Analysis of bacterial genera showed that Salmonella challenge decreased the population of Lactobacillus, the most abundant genus, and increased populations of Pseudomonas and Flavobacterium genera by a factor of 3 to 5. On the other hand, Bacillus administration caused the abundance of the Lactobacillus genus to recover to control levels and decreased the population of Pseudomonas significantly. Further analysis of operational taxonomic units revealed a high abundance of genes associated with two-component systems and secretion systems in the SAL group, whereas the PRO group had more genes associated with ribosomes. Conclusion: The results of this study indicate that B. subtilis CSL2 administration can modulate the microbiota in HLB laying hens, potentially acting as a probiotic to protect against Salmonella Gallinarum infection.

Isolation of Lipase Producing Yeast and Optimization of Cultivation Condition (Lipase 생산 효모균주의 분리 및 배양조건 최적화)

  • 박명훈;류현진;오경근
    • KSBB Journal
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    • v.19 no.2
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    • pp.148-153
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    • 2004
  • Lipase catalyzes the hydrolysis of glycerides into fatty acids and glycerol. The study of microbial lipases has been stimulated in resent years. It is due to the potential uses of lipases in esterification of oils to glycerol, alcohols and carbohydrates. Development of lipase producing yeast has been focused concerning to the utilization of yeast culture for animal feed. In this study, yeast like cells was isolated from a waste oil and sludge. A strain having higher lipase activity was selected by random mutagenesis using UV-radiation. The optimal cultivation conditions in submerged culture were examined in terms of lipase production. 2.0% of high fructose syrup, 1,0% of CSL, and 1.0% of olive oil were selected as the nutritional media for the production of lipase. The maximum lipase activity of 1.12 U/ml and viable cell number of 8.8${\times}$10$\^$7/ cells/mL were obtained at 27$^{\circ}C$ with an initial pH of 5.0.