• Title, Summary, Keyword: CSL

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Optimization for the Bacterial Cellulose Production of Acetobacter xylinum KJ1 by Factorial Design (Factorial design에 의한 Acetobacter xylinum KJ1의 Bacterial cellulose 생산조건의 최적화)

  • 김성준;이지은;정상기;이용운
    • KSBB Journal
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    • v.17 no.3
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    • pp.228-234
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    • 2002
  • Acetobacter xylinum KJ1 efficiently producing bacterial cellulose(BC) In shaking culture was isolated from a rotten grape. The strain was used to investigate optimum operating conditions for increasing BC production and factorial design model was employed for the optimization. The results of experiments were statistically analyzed by SAS program. Reciprocal effects of each factors(carbon source concentration, shaking speeds(rpm), oxygen pressure, and CSL concentration) and culture condition of BC production were examined by getting regression equation of the dependent variable. Comparisons between experimental results and predicted results about BC concentration were done in total 24 experiments by combination of each factors using SAS program, and the correlation coefficients of BC concentration and BC yield were 0.91 and 0.81, respectively. The agitated cultures were peformed in various operation conditions of factors which affected considerably to BC production in jar fermentor. The results showed that BC concentration was 11.67 g/L in 80 hours cultivation under the condition of carbon source concentration : shaking speeds(rpm) : oxygen pressure : CSL concentration : 4% : 460 rpm : 0.28 : 6%. On the other hand BC yield was 0.42 g/g in 80 hours cultivation under the condition of carbon source concentration : shaking speeds(rpm) : oxygen pressure : CSL concentration : 4% : 564 rpm : 0.21 : 2%. The BC production could be enhanced up to more than 2.4 times by factorial design. The result of a verifying experiment under the optimal conditions determined by the factorial design to the BC production showed that the model was appropriate by obtaining BC concentration of 11.47 g/L in the optimum condition.

CSL Computerized Speech Lab - Model 4300B Software version 5.X

  • Ahn, Cheol-Min
    • Proceedings of the KSLP Conference
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    • pp.154-164
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    • 1995
  • CSL, Model 4300B is a highly flexible audio processing package designed to provide a wide variety of speech analysis operations for both new and sophisticated users. Operations include 1) Data acquisition 2) File management 3) Graphics 4) Numerical display 5) Audio output 6) Signal editing 7) A variety of analysis functions, External module include 1) Input control B) Output control 3) Jacks, Software include 1) Wide range of speech display manipulation 2) Editing 3) Analysis (omitted)

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Study of Material Behavior of Concretes Using CSL Model (CSL 모델을 이용한 콘크리트의 재료거동 연구)

  • Zi, Goang-Seup;Jung, Jin-Kyu
    • Proceedings of the Korea Concrete Institute Conference
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    • pp.377-380
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    • 2006
  • The recently developed confinement-shear lattice model is reviewed. The procedure for generating aggregates in a given specimen and the constitutive model for on aggregate-cement strut are shown. It is suggested that the model can easily be extended for early age concretes and fiber reinforced concretes. The state-of-art of the extension and the general procedure of the extension are given in this paper.

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Application of Non-Destructive Testing Techniques to the Evaluation of Integrity of Drilled Shaft (비파괴시험을 이용한 현장타설말뚝의 건전도 평가에 관한 연구)

  • Chae, Jong-Hoon;Yu, Jae-Myung;Kim, Dae-Kyu;Lee, Woo-Jin
    • Journal of the Korean Geoenvironmental Society
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    • v.2 no.4
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    • pp.5-14
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    • 2001
  • The NDT(Non-Destructive Testing) technique, detecting defects without damaging foundations, has, lately, been a matter of concern. In this study, the applicability of the borehole methods(CSL, CT, PS) and the surface reflection methods(SE, IR) to the evaluation of integrity of drilled shaft was investigated through field test. Ten drilled shafts, 0.4 m in diameter and 7.0 m long each, were constructed, one shaft with no defect and nine shafts intentionally with the combination of the common defects such as soft bottom, necking, bulging, cave-in, and/or weak concrete. Analysing each NDP test result on the constructed drilled shafts, an optimum combination of the NDP methods as well as the applicability of each NDP method to detecting defects of drilled shaft have been investigated.

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Culture Condition for the Production of Bacterial Cellulose with Gluconacetobacter persimmonus KJ145 (Giuconacetobacter persimmonus KJ145를 이용한 Bacterial Cellulose 생산조건)

  • Lee, Oh-Seuk;Jang, Se-Young;Jeong, Yong-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.4
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    • pp.572-577
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    • 2002
  • We investigated the optimal condition for production of bacterial cellulose with Gluconacetobacter persimmonus KJ145. For bacterial cellulose production, optimal medium composition and culture conditions were conducted to determine. Apple juice (10$^{\circ}$Brix) medium was suitable than Hestrin & Schramm medium which is generally used for the bacterial cellulose production. When 1% pyruvate as carbon source was added to apple juice, bacterial cellulose production rose to high level. The effect of various nitrogen sources was investigated: CSL was found to be essential to high cellulose yields and the optimal CSL concentration was 10%. Optimal temperature and culture time for the bacterial cellulose production was 35$^{\circ}C$ and 16 days, respectively At the optimal condition Gluconacetobacter persimmonus KJ145 produced 8.96g/L of bacterial cellulose (dry weight), which was much higher than reported values.

Statistical Optimization of Medium Composition for Bacterial Cellulose Production by Gluconacetobacter hansenii UAC09 Using Coffee Cherry Husk Extract - an Agro-Industry Waste

  • Rani, Mahadevaswamy Usha;Rastogi, Navin K.;Anu Appaiah, K.A.
    • Journal of Microbiology and Biotechnology
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    • v.21 no.7
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    • pp.739-745
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    • 2011
  • During the production of grape wine, the formation of thick leathery pellicle/bacterial cellulose (BC) at the airliquid interface was due to the bacterium, which was isolated and identified as Gluconacetobacter hansenii UAC09. Cultural conditions for bacterial cellulose production from G. hansenii UAC09 were optimized by central composite rotatable experimental design. To economize the BC production, coffee cherry husk (CCH) extract and corn steep liquor (CSL) were used as less expensive sources of carbon and nitrogen, respectively. CCH and CSL are byproducts from the coffee processing and starch processing industry, respectively. The interactions between pH (4.5-8.5), CSL (2-10%), alcohol (0.5-2%), acetic acid (0.5-2%), and water dilution rate to CCH ratio (1:1 to 1:5) were studied using response surface methodology. The optimum conditions for maximum BC production were pH (6.64), CSL (10%), alcohol (0.5%), acetic acid (1.13%), and water to CCH ratio (1:1). After 2 weeks of fermentation, the amount of BC produced was 6.24 g/l. This yield was comparable to the predicted value of 6.09 g/l. This is the first report on the optimization of the fermentation medium by RSM using CCH extract as the carbon source for BC production by G. hansenii UAC09.

Logging for a Stone Column Using Crosshole Seismic Testing (크로스홀 탄성파 시험을 이용한 쇄석말뚝의 검측)

  • Kim, Hak-Sung;Mok, Young-Jin
    • International Journal of Highway Engineering
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    • v.12 no.4
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    • pp.139-145
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    • 2010
  • An integrity testing for stone columns was attempted using crosshole S-wave logging. The method is conceptionally quite similar to the crosshole sonic logging (CSL) for drilled piers. The critical difference in the logging is the use of S-wave rather than P-wave, which is used in CSL, because swave is the only wave sensing the stiffness of slower unbounded materials than water. An electro-mechanical source, which can generate reversed Swave signals, was utilized in the logging. The stone column was delineated using the S-wave travel times across the stone column, the S-wave velocity profile of the crushed stone($V_{cs}$-profile) and that of surrounding soil($V_s$-profile). In the calculation of $V_{cs}$-profile of the crushed stone, its friction angle and Ko (coefficient of lateral earth pressure at rest) are recommended to be used. The calculation of the column diameter is not much affected by the values of friction angle and Ko.

Monte Carlo Studies on an Amorphous Silicon (a-Si:H) Digital X-Ray Imaging Device (무정형 실리콘(a-Si : H) 디지털 X-선 영상기기의 개발을 위한 Monte Carlo 컴퓨터 모의실험연구)

  • 이형구;신경섭
    • Journal of Biomedical Engineering Research
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    • v.19 no.3
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    • pp.225-232
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    • 1998
  • Results of Monte Carlo simulations on amorphous silicon based x-ray imaging arrays are described. In order to investigate the characteristics of amorphous silicon x-ray imaging devices and to provide the optimum design parameter, Monte Carlo simulations were performed. Monte Carlo simulation codes for our purpose were developed and various combinations of x-ray peak voltages, aluminum filter thicknesses, CsI(TI) thicknesses, and amorphous silicon photodiode pixel sizes were tested in connection with detection efficiency and spatial resolution of the amorphous silicon based x-ray imager. With usual Csl(TI) thickness of 300${\mu}{\textrm}{m}$-500${\mu}{\textrm}{m}$, detection efficiency was in the range of 70%-95% and energy absorption efficiency was in the range of 40%-70% for 60kVp-120kVp x-ray. From the simulations it was found that amorphous silicon pixel size and Csl(TI) thickness were the most important parameters which determine the resolution of the imager. By use of our simulation results we could provide proper combinations of Csl(TI) thicknesses and pixels sizes for optimum sensitivity and resolution.

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The Effect of Tonsillectomy and Adenoidectomy on Acoustic Factors (구개편도 및 아데노이드 절제술이 음향학적 자질에 미치는 영향)

  • 임성태;손진호;유정운;강지원;이현석;신승헌;박재율
    • Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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    • v.9 no.1
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    • pp.38-42
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    • 1998
  • It has been reported that Tonsillectomy & Adenoidectomy(T & A) resulted in the change of voice by structural changes directly to the vocal track. We studied the effect of T & A on the voice of patients comparing the pre-operative to the post-operative voice. It was performed using a Computerized Speech Lab(CSL50) which is currently used as a method for voice analysis. Forty-five patients who had T&A, aging from 3 to 42 years old, took part in studies and wert evaluated for voice changes and the degree of formant changes of four basic vowels, /a/, /i/, /o/, and /u/. They were evaluated pre-operatively and post-operatively one month later using MDVP, CSL program of CSL50. The results obtained were as follows ; In using MDVP, there were some differences between pre-operative and post-operative shimmer measures within the normal range but other acoustic measures(Fo, jitter, NHR) show no significant differences(p>0.05). F3 of /a/ and /o/ were significantly decreased(p<0.05) and F2, F3 of /i/ were increased(p>0.05) in patients who only had Tonsillectomy in doing CSL spectrogram. For the patients who had T & A, Fl and F3 of /a/, F3 of /i/, Fl, F2 and F3 of /o/ were decreased with significant increase in F1 and F2 of /i/(p<0.05).

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Constitutive Expression of Bacillus stearothermophilus CGTase in Bacillus subtilis. (Bacillus subtilis에서 Bacillus stearothermophilus CGTase의 구성적 발현)

  • 허선연;김중균;권현주;김병우;김동은;남수완
    • Journal of Life Science
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    • v.14 no.3
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    • pp.391-395
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    • 2004
  • To overproduce the cyclodextrin glucanotransferase(CGTase) of Bacillus stearothermophilus NO2 in B. subtilis, the pJH-CGTl plasmid (8.14 kb) was constructed, in which the ORF of CGTase gene could be transcribed by strong constitutive promoter, P$\_$JH/. To overproduce CGTase from a recombinant B. subtilis, the effect of media on the cell growth and expression level of CGTase were investigated with various media (LB, 2${\times}$LB, 5% molasses+2% CSL, CS, LBG) in the flask culture. Among them, [5% molasses+2% CSL] medium revealed the maximum expression level of CGTase with 1.8 unit/$m\ell$ at 9 hr culture. In the batch culture on [10% molasses+5% corn steep liquor] medium the expression level of CGTase, the secretion efficiency, and plasmid stability were about 4.2 unit/$m\ell$, 90% and 90%, respectively, at 30 hr culture. The cell growth and expression level in the fermenter culture with the industrial molasses medium were increased by 2-folds over the flask culture.