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Characteristics on Growth and Amino Acids composition of Quality Protein Maize lines (Quality Protein Maize 계통의 생육 특성 및 아미노산 조성)

  • Bae, Hwan-Hee;Son, Beom-Young;Kim, Jung-Tae;Lee, Jin-Seok;Baek, Seong-Bum
    • Journal of the Korean Society of International Agriculture
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    • v.29 no.3
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    • pp.282-288
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    • 2017
  • To date, many researches have been done for maize QPM lines, there is little maize QPM varieties in Korea. In this reason, to develop maize QPM cultivars, investigation of the growth characteristics and amino acid composition among QPM inbred lines was carried out. For this study, QPM inbred lines were donated from CIMMYT. Analyses of growth characteristics changes for each line were investigated. The range of siliking days were ranged from 60 to 69 days. Silking days for 4 QPM lines such as CML140, CML141, CML152, and CML493 were 60 days among donated QMP lines, CML557 was 69 days. In plant height, CML492 was shortest(152 cm) while CML143 was the longest(295 cm). The ear height of CML492 was 66 cm and 175 cm for CML143. Protein content of each maize QPM grains was from 9.07% to 12.67%. CML556 have the highest proteins content, while CML493 was the lowest among maize QPM grains. Content of fat was from 3.38% to 5.25%, and CML141 was the lowest and CML147 was the highest. The content of ash ranged from 1.22% to 1.78%, CML555 was the least and CML142 was the highest. The content range of carbohydrate was from 71.7% to 75.5%, CML557 was the least and CML493 was the highest. The calories of each inbred line ranged from 346 to 365 Kcal, CML140 was the least and CML492 was the highest. Major amino acid composition in maize QPM grain is glutamic acid (16.34%), proline (9.80%). The mean of lysine composition was 3.64%, and CML140 showed the highest ratio of 4.34%.

Growth Characteristics and Variation in Amino Acids Composition of Quality Protein Maize Lines (고라이신(QPM) 주요 계통과 교잡계의 생육특성 및 아미노산 조성 변이)

  • Bae, Hwan-Hee;Son, Beom-Young;Go, Young-Sam;Park, Hye-Young;Yi, Gibum;Ha, Jun Young;Kim, Mi-Jung;Kim, Sun-Lim;Baek, Seong-Bum
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.3
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    • pp.222-230
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    • 2020
  • Maize grain quality can be improved by raising lysine content, which is an essential amino acid present in insufficient quantities in normal maize. Maize varieties with such modifications are known as quality protein maize (QPM). To date, no Korean maize cultivars contain high amounts of lysine. To introduce quality protein maize to Korean cultivars, we crossed QPM CIMMYT maize lines (CML) with KS140, an elite inbred line used as a parent of several cultivars such as 'Gangdaok' and 'Pyeonganok'. We analyzed the phenotypic characteristics of F1 plants as well as the protein contents, amino acids, and fatty acids profiles of the self-pollinated seeds of the F1 hybrids, and evaluated the feasibility of CML as a source of QPM. Days to anthesis of the CML ranged from 78 to 90 days after planting (DAP), whereas a range of 81~87 was recorded for F1 hybrids. The average days to anthesis was 85 for KS140, CML, and the F1 hybrids. The protein content of the CML was measured to be between 9.1 and 12.1%, with the highest and lowest values being recorded in CML153 and 191, respectively. The F1 hybrids had protein contents of 9.1~11.1%, and the highest content was observed in KS141/CML188. The fatty acids profiles were very similar across all analyzed maize samples, and linoleic acid (C18:2) composed the greatest proportion. Glutamic acid made up the largest proportion of amino acids in all maize samples. Lysine composition was highest in CML155 (6.92% of all amino acids), with an average composition of 4.83% across the CML. In contrast, KS140 showed a lysine content of 2.51%. In F1 hybrids, the average lysine composition was 3.46%, and KS140/CML164 (4.18%) and KS140/CML163 (4.99%) contained more lysine than either parent. Taken together, these results indicate that CML could become promising QPM sources to improve grain quality in Korean maize cultivars.

Evaluation of Drought Tolerance using Anthesis-silking Interval in Maize

  • Kim, Hyo Chul;Moon, Jun-Cheol;Kim, Jae Yoon;Song, Kitae;Kim, Kyung-Hee;Lee, Byung-Moo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.1
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    • pp.24-31
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    • 2017
  • We screened the drought tolerant maize using seventeen maize genotypes from different sources, nine inbred genotypes from United States Department of Agriculture (USDA) (B73, CML103, CML228, CML277, CML322, CML69, Ki3, Ki11, and NC350), three Southeast Asian genotypes (DK9955, LVN-4, and 333), and five Korean hybrids (Cheongdaok, Gangdaok, Ilmichal, Kwangpyeongok, and Pyeonganok). We evaluated anthesis-silking interval (ASI), leaf senescence (LS), ears per plant (EPP), tassel length (TL), and fresh weight (FW) at silking date. According to ASI and LS examination, CML103 and Kill were drought tolerant genotypes, wheareas Ki3 and 333 were drought susceptible. EPP, TL, and FW differed according to drought resistance. Grain yield was correlated strongly with ASI, but moderately with LS. Difference in ASI between drought-stressed (DS) and well-watered (WW) conditions was less than three days in CML228, CML103, Cheongdaok, NC350, B73, Ki11, CML322, and Kwangpyeongok, whereas that of Ki3, Pyeonganok, and Gangdaok was more than 6.5 days. We concluded that CML228, CML103, Cheongdaok, NC350, B73, Ki11, CML322, and Kwangpyeongok are drought tolerant genotypes, whereas Ki3, Pyeonganok, and Gangdaok are drought susceptible.

Clinical Significance of BCR-ABL Fusion Gene Subtypes in Chronic Myelogenous and Acute Lymphoblastic Leukemias

  • Ye, Yuan-Xin;Zhou, Juan;Zhou, Yan-Hong;Zhou, Yi;Song, Xing-Bo;Wang, Jun;Lin, Li;Ying, Bin-Wu;Lu, Xiao-Jun
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.22
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    • pp.9961-9966
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    • 2014
  • Background: Some reports have suggested that chronic myeloid leukemia (CML) patients have a higher prevalence of M-bcr than acute lymphoblastic leukemia (ALL) patients, which show a higher prevalence of m-bcr. However, the relationship between BCR-ABL subtypes and progression of CML and ALL remains unclear. Materials and Methods: 354 CML chronic phase (CML-CP) patients, 26 CML blastic phase (CML-BP) patients and 72 ALL patients before treatment with BCR-ABL positive were recruited for blood routine examination and bone marrow smear cytology. Some 80 CML-CP and 32 ALL patients after imatinib (IM) treatment were followed-up for BCR-ABL relative concentrations detected after treatment for 3, 6 and 9 months and 1 year. Results: Before treatment, CML-CP patients showed lower BCR-ABL relative concentrations with a higher proportion of M-bcr (42.7%) compared to CML-BP and ALL patients while ALL patients had a higher BCR-ABL relative concentration with high expression of m-bcr (51.4%). Patients with M-bcr demonstrated higher WBC counts than those with m-bcr and the mixed group and higher PLT counts were noted in the CML-CP and ALL groups. After imatinib (IM) treatment, patients with m-bcr showed higher BCR-ABL relative concentrations in both CML-CP and ALL groups. Conclusions: This study identified the BCR-ABL gene as an important factor in CML and ALL cases. The M-bcr subtype was associated more with CML while the m-bcr subtype was associated more with ALL. Patients with m-bcr seem to have a poorer response to IM in either CML or ALL patients compared to M-bcr patients.

Web-based Chart Generating System Using CML (CML을 이용한 웹 기반 차트출력시스템)

  • Yoon, Hyun-Nim;Kim, Yang-Woo
    • Journal of Internet Computing and Services
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    • v.9 no.5
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    • pp.47-58
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    • 2008
  • Charts can propagate information more effectively by visualizing various types of information. Due to this reason, many web developers often use charts when they display information on the web. However, using a chart requires a special dedicated software to install and it is difficult to share the finished charts since they are usually in raster format. The raster format images are fixed in size, therefore, experience image distortion problem when their sizes are changed. In this paper, we propose a web-based chart generating system using CML(Chart Markup Language) to solve the current compatibility and sharing problems. The proposed chart generating system first analyzes XML, Text, or HTML documents to extract chart information in the documents, and then it converts collected chart information to CML. The converted CML documents are displayed on the web browser using a vector method. The vector method has an advantage of protecting its images from distortion even when image sizes are scaled. The CML, proposed in this paper, is a chart markup language based on XML for modeling charts Information. If CML is used for presenting a chart in a web, it makes easier to share and to convert the chart information.

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Design Method of Current Mode Logic Gates for High Performance LTPS TFT Digital Circuits (LTPS TFT 논리회로 성능향상을 위한 전류모드 논리게이트의 설계 방법)

  • Lee, J.C.;Jeong, J.Y.
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.44 no.9
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    • pp.54-58
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    • 2007
  • Development of high performance LTPS TFTs contributed to open up new SOP technology with various digital circuits integrated in display panels. This work introduces the current mode logic(CML) gate design method with which one can replace slow CMOS logic gates. The CML inverter exhibited small logic swing, fast response with high power consumption. But the power consumption became compatible with CMOS gates at higher clock speed. Due to small current values in CML, layout area is smaller than the CMOS counterpart even though CML uses larger number of devices. CML exhibited higher noise immunity thanks to its non-inverting and inverting outputs. Multi-input NAND/AND and NOR/OR gates were implemented by the same circuit architecture with different input confirugation. Same holds for MUX and XNOR/XOR CML gates. We concluded that the CML gates can be designed with few simple circuits and they can improve power consumption, chip area, and speed of operation.

Low Level of TERC Gene Amplification between Chronic Myeloid Leukaemia Patients Resistant and Respond to Imatinib Mesylate Treatment

  • Mohamad Ashari, Zaidatul Shakila;Sulong, Sarina;Hassan, Rosline;Husin, Azlan;Sim, Goh Ai;Wahid, S. Fadilah Abdul
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.4
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    • pp.1863-1869
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    • 2014
  • The amplification of telomerase component (TERC) gene could play an important role in generation and treatment of haematological malignancies. This present study was aimed to investigate copy number amplification status of TERC gene in chronic myeloid leukaemia (CML) patients who were being treated with imatinib mesylate (IM). Genomic DNA was extracted from peripheral blood of CML-IM Resistant (n=63), CML-IM Respond (n=63) and healthy individuals (n=30). TERC gene copy number predicted (CNP) and copy number calculated (CNC) were determined based on $Taqman^{(R)}$ Copy Number Assay. Fluorescence in situ hybridization (FISH) analysis was performed to confirm the normal signal pattern in C4 (calibrator) for TERC gene. Nine of CML patients showed TERC gene amplification (CNP=3), others had 2 CNP. A total of 17 CML patients expressed CNC>2.31 and the rest had 2.31>CNC>1.5. TERC gene CNP value in healthy individuals was 2 and their CNC value showed in range 1.59-2.31. The average CNC TERC gene copy number was 2.07, 1.99 and 1.94 in CML-IM Resistant patients, CML-IM Respond and healthy groups, respectively. No significant difference of TERC gene amplification observed between CML-IM Resistant and CML-IM Respond patients. Low levels of TERC gene amplification might not have a huge impact in haematological disorders especially in terms of resistance towards IM treatment.

The rs61764370 Functional Variant in the KRAS Oncogene is Associated with Chronic Myeloid Leukemia Risk in Women

  • Gutierrez-Malacatt, Humberto;Ayala-Sanchez, Manuel;Aquino-Ortega, Xochitl;Dominguez-Rodriguez, Jacqueline;Martinez-Tovar, Adolfo;Olarte-Carrillo, Irma;Martinez-Hernandez, Angelica;Cecilia, Contreras-Cubas C;Orozco, Lorena;Cordova, Emilio J
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.4
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    • pp.2265-2270
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    • 2016
  • Background: Chronic myeloid leukemia (CML) is one of the most frequent hematopoietic malignancies in the elderly population; however, knowledge is limited regarding the genetic factors associated with increased risk for CML. Polymorphisms affecting microRNA (miRNA) biogenesis or mRNA:miRNA interactions are important risk factors in the development of different types of cancer. Thus, we carried out a case-control study to test the association with CML susceptibility of gene variants located in the miRNA machinery genes AGO1 (rs636832) and GEMIN4 (rs2740348), as well as in the miRNA binding sites of the genes BRCA1 (rs799917) and KRAS (rs61764370). Materials and Methods: We determined the genotype of 781 Mexican-Mestizo individuals (469 healthy subjects and 312 CML cases) for the four polymorphisms using TaqMan probes to test the association with CML susceptibility. Results: We found a borderline association of the minor homozygote genotype of the KRAS_rs61764370 polymorphism with an increased risk for CML susceptibility (P = 0.06). After gender stratification, this association was significant only for women (odds ratio [OR] = 13.41, P = 0.04). The distribution of the allelic and genotypic frequencies of the four studied SNPs was neither associated with advanced phases of CML nor treatment response. Conclusions: To the best of our knowledge, this study is the first to show a significant association of the KRAS_rs61764370 SNP with CML. To further determine such an association of with CML susceptibility, our results must be replicated in different ethnic groups.

Chronic Myeloid Leukemia with a Complex Variant 'Ph' Translocation That Develops in Breast Carcinoma, Postchemotherapy: A Rare but Treatable Entity

  • Tikku, Gargi;Jain, Monica;Shukla, Pragya
    • Journal of Breast Cancer
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    • v.20 no.2
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    • pp.208-211
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    • 2017
  • We report a case of chronic myeloid leukemia (CML) that developed after postoperative chemotherapy with cyclophosphamide, doxorubicin and 5-fluorouracil (CAF) for breast cancer. A 55-year-old woman was diagnosed with invasive ductal carcinoma which was treated with a modified radical mastectomy followed by six cycles of CAF chemotherapy. Nine years later, she developed CML and locoregional recurrence. Her breast recurrence showed strong estrogen receptor, weak progesterone receptor and strong human epidermal growth factor 2 (score 3+) expression. Her secondary CML in the chronic phase showed a complex variant translocation (CVT) involving chromosomes 9, 22, and 17. Considering that the HER2/neu gene is also located on chromosome 17, this secondary CML in chronic phase with CVT is indeed a rare occurrence. We discuss the associated genetic factors and the possible role of breast cancer chemo/radiotherapy in the development of such CML as well as its treatment and prognosis compared with de novo CML.

Determination of Nε-(carboxymethyl)lysine in Stir-fried Anchovy Under Different Cooking Conditions (멸치볶음의 조리방법에 따른 Nε-(carboxymethyl)lysine 함량 변화)

  • Jeon, Hyeonjin;Park, Ho-Young;Lee, Sang Hoon;Kim, Yoonsook
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.5
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    • pp.481-486
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    • 2017
  • The content of $N^{\varepsilon}$-(carboxymethyl)lysine (CML), an advanced glycation endproduct, in stir-fried anchovy prepared under different cooking conditions was determined, along with their sensory properties. The aim of the study was to optimize cooking conditions of stir-fried anchovy for minimal CML production by using different sugars, sugar concentrations, pH levels, and cooking times. The results showed that CML content increased with increasing sugar concentration. Cooking time did not affect CML content but lowering the pH with lemon juice decreased it significantly. The CML content of samples with a high sugar concentration, and without lemon juice was $4.67{\mu}g/mL$, and that of samples with both a low sugar concentration and lemon juice was $2.49{\mu}g/mL$, indicating a 47% reduction in the CML content of sir-fried anchovy. We conclude that stir-fried anchovy cooked with less sugar and the addition of lemon juice can reduce CML content while maintaining sensory characteristics.