• Title, Summary, Keyword: CD248

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Increased Expression of P2RY2, CD248 and EphB1 in Gastric Cancers from Chilean Patients

  • Aquea, Gisela;Bresky, Gustavo;Lancellotti, Domingo;Madariaga, Juan Andres;Zaffiri, Vittorio;Urzua, Ulises;Haberle, Sergio;Bernal, Giuliano
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.5
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    • pp.1931-1936
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    • 2014
  • Background: Gastric cancer (GC) ranks as one of the major causes of mortality due to cancer worldwide. In Chile, it is currently the leading cause of cancer death. Identification of novel molecular markers that may help to improve disease diagnosis at early stages is imperative. Materials and Methods: Using whole-genome DNA microarrays we determined differential mRNA levels in fresh human GC samples compared to adjacent healthy mucosa from the same patients. Genes significantly overexpressed in GC were validated by RT-PCR in a group of 14 GC cases. Results: The genes CD248, NSD1, RAB17, ABCG8, Ephb1 and P2RY2 were detected as the top overexpressed in GC biopsies. P2RY2, Ephb1 and CD248 showed the best sensitivity for GC detection with values of 92.9%, 85.7% and 64.3% (p<0.05), respectively. Specificity was 85.7%, 71.4% and 71.4% (p<0.05), for each respectively.

Spectral Response of the n-CdS/n-CdTe/p-CdTe Solar Cells (n-Cds/n-CdTe/p-CdTe 태양전지의 분광반응도)

  • Im, H.B.;Kim, S.J.
    • Proceedings of the KIEE Conference
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    • pp.248-250
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    • 1987
  • Transparent CdS films with low electrical restivity on glass substrates were prepared by coating a CdS slurry which contained 10 wt.% $CdCl_2$, and sintering in a nitrogen atmosphere at $600^{\circ}C$ for 2hr. All-polycrystalline CdS/CdTe solar cells were fabricated by coating CdTe slurries, which contained 1.0 or 4.5 wt.% $CdCl_2$, on the sintered CdS films and sintering at $700^{\circ}C$ for various periods of sintering. The spectral responses of the sintered CdS/CdTe solar cells were measured and compared with theoretically calculated quantum efficiency. The spectral responses of the sintered CdS/CdTe solar cells in the short-wavelength region decreases with-increasing sintering time. The poor response in this region is attributed to the existence of the Cd-S-Te solid solution in the compositional junction. The decrease in the maximum response in the long-wavelength region as the sintering exceeds certain time appears to be caused by the increase in the depth of the buried homo junction and by the increase in the series resistance. The $CdCl_2$ in the CdTe layer during sintering enchances the interdiffusion of S, Te or donor impurities across the metallurgical Junction causing the formation of deeper n-p junction in the CdTe layer.

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Effects of low-level exposure to manganese and lead on immune function (저 농도의 망간과 납 노출이 면역기능에 미치는 영향)

  • Kim, Ki-Woong;Park, SangHwoi;Won, Yong Lim;Lee, Sung Kwang
    • Analytical Science and Technology
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    • v.27 no.5
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    • pp.248-253
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    • 2014
  • This study aimed to evaluate the effects of exposure to manganese (Mn) and lead (Pb) on immune system. The subjects were 42 male workers, among whom 13 office workers (Group I) had never been occupationally exposed to heavy metals, 21 were worked in manufacturing factories (Group II) and 8 were welders (Group III). The mean blood Mn and Pb level by groups were significantly different. The numbers of CD19+ and total lymphocytes in Group I were significantly higher than those in other groups, but no significant differences were found in other T lymphocytes subpopulation. Mn and Pb concentrations showed negative correlation with T lymphocytes subpopulation, but Mn concentrations were statistical significances with T lymphocytes subpoplation except CD4+CD45RO+ and natural killer cell. Pb concentration was only statistical significance with total lymphocytes. Our results suggest that occupationally exposed to Mn and Pb can affect the cellular immune response.

Function and dysfunction of leucine-rich repeat kinase 2 (LRRK2): Parkinson's disease and beyond

  • Bae, Jae Ryul;Lee, Byoung Dae
    • BMB Reports
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    • v.48 no.5
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    • pp.243-248
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    • 2015
  • Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common cause of familial Parkinson's disease (PD). As such, functions and dysfunctions of LRRK2 in PD have been the subject of extensive investigation. In addition to PD, increasing evidence is suggesting that LRRK2 is associated with a wide range of diseases. Genome-wide association studies have implicated LRRK2 in Crohn's disease (CD) and leprosy, and the carriers with pathogenic mutations of LRRK2 show increased risk to develop particular types of cancer. LRRK2 mutations are rarely found in Alzheimer's disease (AD), but LRRK2 might play a part in tauopathies. The association of LRRK2 with the pathogenesis of apparently unrelated diseases remains enigmatic, but it might be related to the yet unknown diverse functions of LRRK2. Here, we reviewed current knowledge on the link between LRRK2 and several diseases, including PD, AD, CD, leprosy, and cancer, and discussed the possibility of targeting LRRK2 in such diseases. [BMB Reports 2015; 48(5): 243-248]

The effect of intracellular trafficking of CD1d on the formation of TCR repertoire of NKT cells

  • Shin, Jung Hoon;Park, Se-Ho
    • BMB Reports
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    • v.47 no.5
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    • pp.241-248
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    • 2014
  • CD1 molecules belong to non-polymorphic MHC class I-like proteins and present lipid antigens to T cells. Five different CD1 genes (CD1a-e) have been identified and classified into two groups. Group 1 include CD1a-c and present pathogenic lipid antigens to ${\alpha}{\beta}$ T cells reminiscence of peptide antigen presentation by MHC-I molecules. CD1d is the only member of Group 2 and presents foreign and self lipid antigens to a specialized subset of ${\alpha}{\beta}$ T cells, NKT cells. NKT cells are involved in diverse immune responses through prompt and massive production of cytokines. CD1d-dependent NKT cells are categorized upon the usage of their T cell receptors. A major subtype of NKT cells (type I) is invariant NKT cells which utilize invariant $V{\alpha}14-J{\alpha}18$ TCR alpha chain in mouse. The remaining NKT cells (type II) utilize diverse TCR alpha chains. Engineered CD1d molecules with modified intracellular trafficking produce either type I or type II NKT cell-defects suggesting the lipid antigens for each subtypes of NKT cells are processed/generated in different intracellular compartments. Since the usage of TCR by a T cell is the result of antigen-driven selection, the intracellular metabolic pathways of lipid antigen are a key in forming the functional NKT cell repertoire.

Electrokinetics를 이용한 김에서의 중금속 제거

  • 이현호;서성준;최관영;양지원
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • pp.245-248
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    • 1998
  • Electrokinetic tests are conducted on laver contaminated with heavy metals to investigate the efficiency of the process in removing Cd, Pb, and Zn. The tests were operated at constant current, and operating time were 0 - 24 hours. The removal efficiency in electro-osmosis with open electrodes is time-dependent. The absorbed Cd, Pb and Zn were removed 50.94-95.75%, 80.78-81.96%, and 48.10-83.83% by the process.

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Dark Conductivity in Semi-Insulating Crystals of CdTe:Sn

  • Makhniy, V.P.;Sklyarchuk, V.M.;Vorobiev, Yu.V.;Horley, P.P.
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.15 no.2
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    • pp.243-248
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    • 2015
  • We prepared semi-insulating CdTe for radiation detectors by isothermal annealing of single crystals grown by Bridgeman technique in a sealed quartz container filled with Sn vapor. The resistivity of CdTe:Sn samples thus obtained was of order of $10^{10}Ohm{\cdot}cm$ at room temperature with electrons lifetime of $2{\times}10^{-8}$ s, which is appropriate for the applications desired. Analysis of electric transport characteristics depending on temperature, sample thickness and voltage applied revealed the presence of traps with concentration of about $(4-5){\times}10^{12}cm^{-3}$ with the corresponding energy level at 0.8 - 0.9 eV counted from the bottom of conduction band. The conductivity was determined by electron injection from electrodes in space charge limited current mode.

Emerging Surgical Strategies of Intractable Frontal Lobe Epilepsy with Cortical Dysplasia in Terms of Extent of Resection

  • Shin, Jung-Hoon;Jung, Na-Young;Kim, Sang-Pyo;Son, Eun-Ik
    • Journal of Korean Neurosurgical Society
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    • v.56 no.3
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    • pp.248-253
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    • 2014
  • Objective : Cortical dysplasia (CD) is one of the common causes of epilepsy surgery. However, surgical outcome still remains poor, especially with frontal lobe epilepsy (FLE), despite the advancement of neuroimaging techniques and expansion of surgical indications. The aim of this study was to focus on surgical strategies in terms of extent of resection to improve surgical outcome in the cases of FLE with CD. Methods : A total of 11 patients of FLE were selected among 67 patients who were proven pathologically as CD, out of a total of 726 epilepsy surgery series since 1992. This study categorized surgical groups into three according to the extent of resection : 1) focal corticectomy, 2) regional corticectomy, and 3) partial functional lobectomy, based on the preoperative evaluation, in particular, ictal scalp EEG onset and/or intracranial recordings, and the lesions in high-resolution MRI. Surgical outcome was assessed following Engel's classification system. Results : Focal corticectomy was performed in 5 patients and regional corticectomy in another set of 5 patients. Only 1 patient underwent partial functional lobectomy. Types I and II CD were detected with the same frequency (45.45% each) and postoperative outcome was fully satisfactory (91%). Conclusion : The strategy of epilepsy surgery is to focus on the different characteristics of each individual, considering the extent of real resection, which is based on the focal ictal onset consistent with neuroimaging, especially in the practical point of view of neurosurgery.

Platelet-Derived Growth Factor Receptor-Positive Pericytic Cells of White Adipose Tissue from Critical Limb Ischemia Patients Display Mesenchymal Stem Cell-Like Properties

  • Kim, Eo Jin;Seo, Sang Gyo;Shin, Hyuk Soo;Lee, Doo Jae;Kim, Ji Hye;Lee, Dong Yeon
    • Clinics in Orthopedic Surgery
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    • v.9 no.2
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    • pp.239-248
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    • 2017
  • Background: The pericytes in the blood vessel wall have recently been identified to be important in regulating vascular formation, stabilization, remodeling, and function. We isolated and identified pericyte-like platelet-derived growth factor receptor betapositive ($PDGFR{\beta}+$) cells from the stromal vascular fraction (SVF) of adipose tissue from critical limb ischemia (CLI) patients and investigated their potential as a reliable source of stem cells for cell-based therapy. Methods: De-identified subcutaneous fat tissues were harvested after amputation in CLI patients. Freshly isolated SVF cells and culture-expanded adipose-derived stem cells (ADSCs) were quantified using flow cytometry. A matrigel tube formation assay and multi-lineage differentiation were performed to assess pericytic and mesenchymal stem cell (MSC)-like characteristics of $PDGFR{\beta}+$ ADSCs. Results: $PDGFR{\beta}+$ cells were located in the pericytic area of various sizes of blood vessels and coexpressed mesenchymal stem cell markers. $PDGFR{\beta}+$ cells in freshly isolated SVF cells expressed a higher level of stem cell markers (CD34 and CXCR4) and mesenchymal markers (CD13, CD44, CD54, and CD90) than $PDGFR{\beta}-$ cells. In vitro expansion of $PDGFR{\beta}+$ cells resulted in enrichment of the perivascular mesenchymal stem-like ($PDGFR{\beta}+$/CD90+/CD45-/CD31-) cell fractions. The Matrigel tube formation assay revealed that $PDGFR{\beta}+$ cells were located in the peritubular area. Conclusions: $PDGFR{\beta}+$ ADSCs cells demonstrated a good multilineage differentiation potential. Pericyte-like $PDGFR{\beta}+$ cells from the SVF of adipose tissue from CLI patients had MSC-like characteristics and could be amplified by in vitro culture with preservation of their cell characteristics. We believe$PDGFR{\beta}+$ cells in the SVF of adipose tissue can be used as a reliable source of stem cells even in CLI patients.

Comparing Bioavailability of Cadmium and Arsenic in Agricultural Soil Under Varied pH Condition

  • Oh, Se Jin;Kim, Sung Chul;Ok, Yong Sik;Oh, Seung Min;Lee, Bup Yeol;Lee, Sang Hwan;Yang, Jae E.
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.1
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    • pp.57-63
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    • 2015
  • Bioavailability of cadmium (Cd) and arsenic (As) can be different depending on soil pH. For this reason, main purpose of this research was to compare bioavailability of Cd and As in agricultural field under varied soil pH and different extractants. Bioavailable fraction of Cd and As in soil was extracted with $CaCl_2$, $NaNO_3$, DTPA, EDTA, and low molecular weight organic acids (LMWOAs). Soil samples and cultivated crops were collected at the range of soil pH 4.5-8.5 and correlation analysis was conducted between bioavailable fraction of Cd and As in soil and total concentration of Cd and As in crops. Results showed that concentration of Cd and As in acidic soil was ranged $0.002-0.462mg\;kg^{-1}$ and $0.041-4.903mg\;kg^{-1}$ respectively. In alkaline condition, concentration of Cd and As were ranged $0.006-0.351mg\;kg^{-1}$ and $0.039-2.807mg\;kg^{-1}$ respectively. Comparing bioavailable fraction of Cd and As in acidic and alkaline soil condition, higher concentration was measured in acidic condition. Similarly, higher average concentration of Cd and Asin crops was observed in acidic condition (0.398 and $0.751mg\;kg^{-1}$ respectively) than alkaline condition (0.248 and $0.264mg\;kg^{-1}$). Among different extractants, LMWOAs method showed higher correlation ($r^2=0.545$) for Cd in acidic condition indicating that LMWOAs method could be applied for evaluating bioavailability of Cd in acidic soil. However, no high correlation was observed for As in both acidic and alkaline condition. Overall, bioavailable fraction of Cd and As can be higher in acidic condition of soil than alkaline condition resulting higher uptake of Cd and As from soil to crops. Therefore, efficient best management practice (BMPs) for Cd and As in acidic soil should be conducted for minimizing uptake of Cd and As into crops.