• Title, Summary, Keyword: Bubble Column

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Effect of Inorganic Salts and Various Bioreactors on the Production of Clavulanic Acid (무기염과 생물반응기의 종류가 Clavulanic acid의 생산에 미치는 영향)

  • Kim, Il-Chul;Kim, Seung-Uk
    • KSBB Journal
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    • v.14 no.4
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    • pp.440-444
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    • 1999
  • For the effecient production of clavulanic acid., a mutant strain Streptomyces clavuligerus KK was selected from Streptomyces clavuligerus ATCC 27064 through mutation with NTG. S. clavuligerus ATCC 27064 produced about 200 mg/L of calvulanic acid when the medium was composed of 1%(W/V) glycerol, 1.5%(W/V) soybean flour, 0.1%(W/V) $KH_2PO_4$, 0.2%(V/V) soybean oil. A selected mutant, S. clavuligerus KK, produced about 1150 mg/L of clavulanic acid in the same medium. After the addition of $MgSO_4$ to the basal medium, S. clavuligerus KK produced about 1550 mg/L of clavulanic acid, with shows about 1.3 times higher than that produced in the basal medium. In order to select the proper bioreactor for the production of clavulanic acid, a batch culture was performed in an airlift, a bubble column and an stirred tank bioreactors. In an airlift bioreactor, about 1350 mg/L of clavulanic acid was produced, in a bubble column bioreactor, about 1550 mg/L, in a stirred tank bioreactor, about 2200 mg/L, respectively. The production of clavulanic acid in stirred tank bioreactor was about 50% higer than that by an airlift and a bubble column bioreactors. According to this result, the stirred tank bioreactor was selected as a proper bioreactor.

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Development of a Novel Bioreactor System for the Treatment of Gaseous Benzene

  • Yeom, Sung-Ho;Daugulis, Andrew J.;Yoo, Young-Je
    • 한국생물공학회:학술대회논문집
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    • pp.73-76
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    • 2000
  • A novel, continuous bioreactor system combining a bubble column (absorption section) and a two-phase bioreactor (degradation section) has been designed to treat a gas stream containing benzene. The bubble column contained hexadecane as an absorbent for benzene, and was systemically chosen considering physical, biological, environmental, operational and economic factors. This solvent has infinite solubility for benzene and very low volatility. After absorbing benzene in the bubble column, the hexadecane served as the organic phase of the two-phase partitioning bioreactor, transferring benzene into the aqueous phase where it was degraded by Alcaligenes xylosoxidans Y234. The hexadecane was then continuously recirculated back to the absorber section for the removal of additional benzene. All mass transfer and biodegradation characteristics in this system were investigated prior to operation of the integrated unit, and these included: the mass transfer rate of benzene in the absorption column, the mass transfer rate of benzene from the organic phase into the aqueous phase in the two-phase bioreactor, the stripping rate of benzene out of the two-phase bioreactor, etc. All of these parameters were incorporated into model equations, which were used to investigate the effects of operating conditions on the performance of the system. Several experiments were conducted to show the feasibility of this system. This process is believed to be very practical for the treatment of high concentrations of gaseous pollutants.

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Effects of Operating Variables on the Conversion of Meta Boric Acid Formation in a Bubble Column Reactor (기포탑 반응기에서 조작변수가 meta 붕산 생성반응 전환율에 미치는 영향)

  • Cho, Soo-Haeng;Do, Jae-Bum;Kang, Y.
    • Applied Chemistry for Engineering
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    • v.7 no.3
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    • pp.573-579
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    • 1996
  • Effects of operating variables on the conversion of the formation reaction of meta boric acid from ortho boric acid in a laboratory-scale bubble column reactor were investigated to obtain the basic data which are indispensable for the design, scale-up, control, development and operation of industrial bubble column reactors. Reaction time and pressure, particle size of reactant and gas flow rate were chosen as experimental variables. Effects of the experimental variables on the gas holdup in the bubble column reactor were also discussed in relation to the conversion of reaction. From the results of this study, the optimum conditions were drawn as follows : Reaction time ; 35~40(min), reaction pressure ; 92~95(kPa), particle size ; under $0.6{\times}10^{-3}(m)$, gas flow rate ; 0.07~0.08(m/s).

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Methane Recovery and Carbon Dioxide Stripping by MEA Solution the Autocirculation Bubble Lift Column Reactor (내부순환식 기포탑 반응기 상에서 MEA (monoethanolamine) 용액에 의한 이산화탄소 분리 및 메탄회수)

  • Lee, In-Hwa;Kim, Sun-Yil;Park, Ju-Young
    • Applied Chemistry for Engineering
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    • v.18 no.3
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    • pp.239-244
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    • 2007
  • For the simultaneous methane recovery and $CO_2$-stripping, we have been developed dual vent auto circulation bubble lift column reactor, and evaluate optimum conditions for monoethanolamine (MEA) solutions as a $CO_2$ absorbent. At the 5 wt% MEA solution, we investigated the pH change during $CO_2$-stripping and absorption reaction, $CO_2$-stripping rate with reaction time, methane recovery efficiency for various inflow rates of air, $CO_2$-stripping rate for flow liquid over flow height, and $CO_2$-stripping dependency on the temperature of absolvent solutions. The suggested optimum conditions for $CO_2$ recovery with MEA in the dual vent auto circulation bubble lift column reactor were 40 mm over flow liquid height, 1.5 L/min of air inflow rate, and $25^{\circ}C$ of absorbent solution temperature.

Design of Recycle Bubble Column Reactor for Continuous Enzymatic Hydrolysis of Cellulose (섬유소의 연속 효소 가수분해를 위한 순환식 기포탑 반응기의 설계)

  • 김춘영;홍석표정봉우이태원
    • KSBB Journal
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    • v.5 no.1
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    • pp.59-67
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    • 1990
  • Enzymatic hydrolysis of insoluble cellulose was performed in a bubble column with tangential flow ulrafiltration membrane unit. The reactor was operated in a batch mode as well as semi-continuous and continuous with continuous removal of products through the tangential flow ultrafiltration membrane. The optimum superficial gas velocity was 1-3cm / sec so as to avoid bubble coalescence and enzyme denaturation. In continuous and selni-cotinuous process, the conversion was gradually increased but the total reduced sugar concentration was drcastically dereased with the dilution rate. It was concluded that the bubble column attaching tangential flow ultrafiltration membrane unit was effective on continuous hydrolysis of cellulose and recovery of enzyme.

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Fractional gas hold-up in trayed bubble column (Trayed 기포탑 반응기에서 높이에 따른 기포입자의 거동분석)

  • Yang, Jung Hoon;Hur, Young Gul;Yang, Jung-Il;Kim, Hak-Joo;Chun, Dong Hyun;Kim, Byung Kwon;Lee, Ho-Tae;Jung, Heon
    • 한국신재생에너지학회:학술대회논문집
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    • pp.77.1-77.1
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    • 2011
  • 슬러리 기포탑 반응기는 열 및 물질 전달의 용이성, 낮은 운전비용 및 장치의 간단성의 장점을 가지고 있어서 Fischer-Tropsch 반응, bio-reaction 등에 많이 응용되고 있다. 특히, 반응물은 기체 상태로 반응기에 투입이 되는데, 이 기포입자의 상승하는 힘을 바탕으로 기상/액상/고상이 균일하게 혼합되게 된다. 많은 연구자들이 이러한 기포탑 반응기의 성능을 개선하고자, 다양한 반응기 디자인에 대하여 보고하고 있다. 특히 반응기 내부에 tray를 설치함으로써, 기포 포집율을 증진시기고 액상의 역류를 최소화시키는 연구가 활발히 진행되고 있다. 본 연구에서는, 다양한 기공크기를 갖는 tray를 활용함으로써 높이에 따른 기포 포집율의 변화 및 반응기 내에 기포 입자의 거동 특성에 대하여 살펴보았다.

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Bubble Properties in Bubble Columns with Electrolyte Solutions (전해질용액 기포탑에서 기포특성)

  • Yoo, D.J.;Lim, D.H.;Jeon, J.S.;Yang, S.W.;Kang, Y.
    • Korean Chemical Engineering Research
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    • v.54 no.4
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    • pp.543-547
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    • 2016
  • Bubble properties such as size (chord length) and rising velocity were investigated in a bubble column with electrolyte solutions, of which diameter was 0.152m and 2.5m in height, respectively. The size and rising velocity of bubbles were measured by using the dual electrical resistivity probe method. Effects of gas and liquid velocities and ionic strength of liquid phase on the size and rising velocity of bubbles were determined. The bubble size increased with increasing gas velocity but decreased with increasing liquid velocity or ionic strength of liquid phase. The rising velocity of bubbles increased with increasing gas velocity and decreased with increasing ionic strength of liquid phase, however, it showed a slight maximum value with varying liquid velocity. The size and rising velocity of bubbles were well correlated with operating variables.

Study on Effect of gas superficial velocity on particle behavior in three phased Slurry Bubble Column Reactor (3상 Slurry Bubble Column Reactor에서 기체유속에 따른 고체입자의 거동에 대한 연구)

  • Yang, Jung-Hoon;Yang, Jung-Il;Lee, Ho-Tae;Kim, Hak-Joo;Chun, Dong-Hyun;Jung, Heon
    • 한국신재생에너지학회:학술대회논문집
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    • pp.876-879
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    • 2009
  • Fischer-Tropsch 합성 반응과 같은 slurry bubble column reactor에서는 반응 속도를 증진시키기 위해서는 서로 다른 상간의 접촉 면적을 최대화함으로써 물질 전달을 원활하게 유지하여야 한다. 특히 Fischer-Tropsch 합성 반응에서는 반응물인 기체가 촉매로서 기능하는 고체 표면으로의 external mass transfer가 효과적으로 이루어져야 하기 때문에 반응기 내의 기체의 거동뿐만 아니라 고체인 촉매의 분포에 대한 연구가 활발하게 이루어지고 있다. 따라서 본 연구에서는 반응기 내에 기체의 superficial velocity를 변화시키면서 기체의 hold up 뿐만 아니라 고체 입자의 분포특성에 대하여 관찰하였다. Superficial velocity가 증가함에따라 gas hold up의 경우, 일정하게 증가하다가 6 cm/sec 이상에서 그 증가폭이 감소하였다. 즉 6 cm/sec이상에서 turbulent flow regime을 형성하였다. 또한 고체입자의 분포 역시 기체의 superficial velocity가 증가함에따라 보다 균일하게 되는 것을 확인할 수 있었다.

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Evaluation and modelling of the separation of anthracite in the hindered-settling column

  • Kim, Jeong-Yun;Cho, Hee-Chan
    • 한국지구물리탐사학회:학술대회논문집
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    • pp.611-617
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    • 2003
  • This study was performed to develop the method for producing industrial coal sources by cleaning Korean anthracite. Laboratory hindered-settling separation column was set and three coal samples were used for tests. Tests were conducted to evaluate the effects of the major operating variables, teeter water flow rate and relative column pressure (set point). Additional tests were performed to elevate the yield and properties of the products using air bubble injecting process. In results, nice products were obtained with high teeter water flow rate and air bubble injection. Also, model of continuous hindered-settling separation process was established to assist the evaluation of the equipment and several operating variables, such as dispersion, teeter water flow rate, feeding rate, etc.

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Heat Transfer Model and Energy Dissipation Rate in Bubble Columns with Continuous Operation (연속조작 기포탑에서 열전달 모델 및 에너지 소멸 속도)

  • Jang, Ji Hwa;Seo, Myung Jae;Lim, Dae Ho;Kang, Yong;Jung, Heon;Lee, Ho Tae
    • Korean Chemical Engineering Research
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    • v.47 no.5
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    • pp.587-592
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    • 2009
  • Heat transfer model and energy dissipation rate were investigated to examine the heat transfer mechanism in bubble columns with continuous operation. The energy dissipation rate($E_D$) obtained from the unsteady state heat transfer model based on the surface renewal theory was significantly small, comparing with the hydrodynamic energy dissipation rate($P_v$) calculated from the overall hydrodynamic energy balance based on the behaviors and holdups of gas and liquid phases in the column. It was found from these results that the energy dissipation rate based on the surface renewal theory is independent of the hydrodynamic energy dissipation rate obtained from the overall hydrodynamic energy balance in the bubble column, in considering their mechanism. The different two energy dissipation rates were correlated in terms of operating variables within this experimental conditions, respectively.