• Title/Summary/Keyword: Bubble Column

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The Growth of Transgenic Tobacco′s Suspension Culture and the Production of β-Glucuronidase in Bubble Column Bioreactor (Bubble column bioreactor에서 형질전환된 담배세포의 성장양상 및 β-Glucuronidase의 생산)

  • 김석우;이동근;현진원;이상현;하종명;하배진;이재화
    • Journal of Life Science
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    • v.12 no.5
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    • pp.577-583
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    • 2002
  • The growth kinetics and the production of $\beta$-glucuronidase from transgenic tobacco's suspension culture was investigated in the flask culture and a 2.5 L bubble column reactor. The growth of bubble column reactor was similar to that of flask culture. However, in the bubble column reactor, the production of $\beta$ -glucuronidase reached 2850 U/mg (85-fold higher than that of flask culture). In both case, the production level of $\beta$ -glucuronidase was fluctuated, which was resulted from periodical degradation of the protein. Sucrose is important component in plant culture medium. Twice addition of sucrose in bubble column reactor could not improve cell growth, since other components in a medium were already depleted. However, the addition of sugar decreased cell size, which facilitated the operation of bioreactor. The production of $\beta$ -glucuronidase was continuously increased, however final concentration of $\beta$ -glucuronidase was similar to that without sucrose addition.

Effects of Various Bioreactors on Growth and Ginsenoside Accumulation in Ginseng Adventitious Root Cultures(Panax ginseng C.A. Meyer) (다양한 생물반응기 형태가 인삼(Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 생산에 미치는 영향)

  • Kim, Yun-Soo;Hahn, Eun-Joo;Paek, Kee-Yoeup
    • Journal of Plant Biotechnology
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    • v.31 no.3
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    • pp.249-253
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    • 2004
  • The type of air lift bioreactor affected the root growth in ginseng adventitious root cultures. Among bioreactors used in this experiment, bulb type bubble bioreactor (BU) was the best to increase root growth (41.92 g dry weight). The kLa value representing the oxygen transfer capacity from medium to explants (6.98 h$^{-1}$ ) in BU with 5 cm bubble column was higher than other bioreactors. On the other hand, cylindric tube bioreactor (CT) without bubble column resulted in minimum root growth (38.55 g dry weight) and kLa value (5.25 h$^{-1}$ ). Furthermore, the root growth (50.30 g dry weight) in BU with 10 cm bubble column more increased than 5 cm bubble column. However, the kLa value do not affected the secondary metabolite such as ginsenosides. These results show that the bubble column in air lift bioreactor increase kLa value and increased kLa value stimulate the growth of ginseng adventitious roots.

Bubble-column Photobioreactor에서의 Astaxanthin 유도배양의 연구

  • Choe, Su-Rim;Seo, In-Su;Lee, Cheol-Gyun
    • 한국생물공학회:학술대회논문집
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    • pp.220-223
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    • 2002
  • This study investigated a lab-scale inducing method for efficient astaxanthin accumulation. As a model system. Haematococcus pluvialis was cultivated in 2 liter bubble-column photobioreactors. The astaxanthin - inducing results using high light irradiation were compared with that of the control experiment under standard irradiation (40 ${\mu}E/m^2/s$). After the late linear growth phase (> 20 days). high light energy (230 ${\mu}E/m^2/s$) was supplied to the culture broth for astaxanthin induction. As a result. the dr γ cell weight and the astaxanthin productivity were increased up to 68% and 215%, respectively. higher than those of the control experiment. This result indicates that bubble-column type photobioreactor is a good candidate for mass cultivation of H. pluvialis and high light irradiation is an efficient induction method for astaxanthin accumulation in lab-scale bubble-column photobioreactors.

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Effect of Aspergillus niger Pellets on Citric Acid Production in a Bubble Column Bioreactor

  • Kim, Seung-Hwan;Yoo, Young-Je;Kim, Eui-Yong;Kim, Min-Hong
    • Journal of Microbiology and Biotechnology
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    • v.5 no.3
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    • pp.172-176
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    • 1995
  • Citrate is mainly produced from fungi and oxygen transfer has been known as one of the important factors in citric acid production. A bubble column bioreactor was used for citrate production after pellet was initially made using a stirred bioreactor for the inoculation. The relationship between the pellet size of Aspergillus niger and the oxygen transfer was elucidated by considering morphological characteristics of the pellet. The pellet size was determined by adjusting the impeller speed in the stirred bioreactor and the optimum diameter of the pellet was observed to be 2.2 mm under the experimental conditions. Pellet was maintained quite stable in the bubble column bioreactor and production of citric acid was significantly improved by maintaining optimal pellet conditions in the bubble column bioreactor.

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Comparative Study of Mass Transfer and Bubble Hydrodynamic Parameters in Bubble Column Reactor: Physical Configurations and Operating Conditions

  • Sastaravet, Prajak;Chuenchaem, Chomthisa;Thaphet, Nawaporn;Chawaloesphonsiya, Nattawin;Painmanakul, Pisut
    • Environmental Engineering Research
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    • v.19 no.4
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    • pp.345-354
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    • 2014
  • In this paper, effects of physical configurations and operating conditions on bubble column performance were analyzed in terms of bubble hydrodynamic and mass transfer parameters. Bubble column with 3 different dimensions and 7 gas diffusers (single / multiple orifice and rigid / flexible orifice) were applied. High speed camera and image analysis program were used for analyzing the bubble hydrodynamic parameters. The local liquid-side mass transfer coefficient ($k_L$) was estimated from the volumetric mass transfer coefficient ($k_La$) and the interfacial area (a), which was deduced from the bubble diameter ($D_B$) and the terminal bubble rising velocity ($U_B$). The result showed that the values of kLa and a increased with the superficial gas velocity (Vg) and the size of bubble column. Influences of gas diffuser physical property (orifice size, thickness and orifice number) can be proven on the generated bubble size and the mass transfer performance in bubble column. Concerning the variation of $k_L$ coefficients with bubble size, 3 zones (Zone A, B and C) can be observed. For Zone A and Zone C, a good agreement between the experimental and the predicted $K_L$ coefficients was obtained (average difference of ${\pm}15%$), whereas the inaccuracy result (of ${\pm}40%$) was found in Zone B. To enhance the high $k_La$ coefficient and absorption efficiency in bubble column, it was unnecessary to generate numerous fine bubbles at high superficial gas velocity since it causes high power consumption with the great decrease of $k_L$ coefficients.

Continuous rapid Production of Soy Sauce by Coimmobilized Mixed Culture system of Zygosaccharomyces rouxii and Candida versatilis using Air Bubble Column Reactor (Zygosaccharomyces rouxii와 Candida versatilis의 동시 고정화에 의한 Air Bubble Column Reactor에서 간장의 연속적 속성 생산)

  • 류병호
    • KSBB Journal
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    • v.18 no.6
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    • pp.455-460
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    • 2003
  • This study was designed to find out the rapid fermentation of soy sauce from koji hydrolyzates using air bubble column reactor packed with coimmobilized mixed culture system. Continuous ripid production was performed by coimmobilized Z. rouxiii BH-90 and C. versatilis BH-91. Coimmobilized cells of Zygosaccharomyces rouxii BH-90 and Candida versatilis BH-91 mixture cells in the column reactor produced 2.8% ethyl alcohol and 18mg/L 4-ethylguaiacol over 96 hours under the optimal conditions. Coimmobilized cells produced 2.30∼2.4% ethyl alcohol during 30 days, and decreased gradually from 40 days to 70 days. Also coimmobilized cells produced 4-ethylguaiacol at the constant rate of 16∼18mg/L and decreased gradually after 40 days. Final product of soy sauce contained 2.4% ethyl alcohol and 18mg/L 4-ethylguaiacol. However, amino acid compositions of soy sauce were consisted of predominantly glutamic acid, leucin, arginine, aspartic acid, Iysine and valine, which were more than 50% of total amino acid.

Characteristics of Gas-liquid Mass Transfer and Interfacial Area in a Bubble Column

  • Lim, Dae Ho;Yoo, Dong Jun;Kang, Yong
    • Korean Chemical Engineering Research
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    • v.53 no.3
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    • pp.315-320
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    • 2015
  • Characteristics of gas-liquid mass transfer and interfacial area were investigated in a bubble column of diameter and height of 0.102 m and 2.5 m, respectively. Effects of gas and liquid velocities on the volumetric gas-liquid mass transfer coefficient ($k_La$), interfacial area (a) and liquid side true mass transfer coefficient ($k_L$) were examined. The interfacial area and volumetric gas-liquid mass transfer coefficient were determined directly by adopting the simultaneous physical desorption of $O_2$ and chemical absorption of $CO_2$ in the column. The values of $k_La$ and a increased with increasing gas velocity but decreased with increasing liquid velocity in the bubble column which was operated in the churn turbulent flow regime. The value of $k_L$ increased with increasing gas velocity but did not change considerably with increasing liquid velocity. The liquid side mass transfer was found to be related closely to the liquid circulation as well as the effective contacting frequency between the bubbles and liquid phases.

Design Parameters Estimations for Bubble Column Reactors to Remove Toxic Gases (독성가스 제거용 기포탑 반응기의 설계기법)

  • Oh, Junghwan;Hong, Min Sun
    • Korean Journal of Hazardous Materials
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    • v.6 no.2
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    • pp.99-108
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    • 2018
  • Gas-liquid bubble column reactors are extensively used in industrial processes. A detailed knowledge of bubble size distribution is needed for determining the mass transfer in gas-liquid film. Experimental data on bubble size distribution and liquid-side mass transfer coefficient($k_L$) were used to calculate the estimated time to saturation in bubble column reactor. Also, the gas flux was evaluated to the liquid-side mass transfer coefficient($k_L$) and solubility data for hydrogen sulfide($H_2S$) and chlorine($Cl_2$) absorption into water. Simulation results show that $H_2S$ absorption time to 50 % of saturation concentrations are 611 sec and 1,329 sec when bubble diameters are 0.5 mm and 4.5 mm, while absorbing 1 % $H_2S$ gas. In case of $Cl_2$, absorption time range 657 to 1,400 sec when bubble size range 0.5 mm to 4.5 mm, while absorbing 1 % $Cl_2$ gas. Calculated simulation results can be used in the design of emergency relief bubble reactors.

The Effect of Partitioning Porous Plate on Bubble Behavior and Gas Hold-up in a Bench Scale (0.36 m × 22 m) Trayed Bubble Column (벤치스케일(0.36 m × 22 m) 다단형 기포탑에서 다공판이 기포의 거동 및 기체 체류량에 미치는 영향)

  • Yang, Jung Hoon;Hur, Young Gul;Lee, Ho-Tae;Yang, Jung-Il;Kim, Hak-Joo;Chun, Dong Hyun;Park, Ji Chan;Jung, Heon
    • Korean Chemical Engineering Research
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    • v.50 no.3
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    • pp.505-510
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    • 2012
  • The gas hold-up has a strong relationship with the size distribution and rising velocities of bubbles in a bubble column. Therefore, many previous researchers have studied on the hydrodynamics focusing on the bubble size variation in bubble column. In this study, the bubble behavior was influenced by partitioning porous plates installed at a certain height in a trayed bubble column. The gas hold-up was increased in non-sparging region (H/D > 5) as well as sparging region. We identified the effect of the partitioning porous plate using three trayed bubble columns with different reactor geometries. Furthermore, the bubble break-up frequency and size distribution were observed before and after individual bubbles penetrated through the plate. The arrangement of the plates was also investigated using a 0.15-m-in-diameter bubble column. Based on the result, we applied this design concept to a 0.36-m-in-diameter, 22 m tall trayed bubble column and identified the effect of the partitioning porous plate on the gas hold-up increase.