• Title, Summary, Keyword: Breast

Search Result 6,369, Processing Time 0.055 seconds

Panduratin A Inhibits Cell Proliferation by Inducing G0/G1 Phase Cell Cycle Arrest and Induces Apoptosis in Breast Cancer Cells

  • Liu, Qiuming;Cao, Yali;Zhou, Ping;Gui, Shimin;Wu, Xiaobo;Xia, Yong;Tu, Jianhong
    • Biomolecules & Therapeutics
    • /
    • v.26 no.3
    • /
    • pp.328-334
    • /
    • 2018
  • Because of the unsatisfactory treatment options for breast cancer (BC), there is a need to develop novel therapeutic approaches for this malignancy. One such strategy is chemotherapy using non-toxic dietary substances and botanical products. Studies have shown that Panduratin A (PA) possesses many health benefits, including anti-inflammatory, anti-bacterial, anti-oxidant and anticancer activities. In the present study, we provide evidence that PA treatment of MCF-7 BC cells resulted in a time- and dose-dependent inhibition of cell growth with an $IC_{50}$ of $15{\mu}M$ and no to little effect on normal human MCF-10A breast cells. To define the mechanism of these anti-proliferative effects of PA, we determined its effect critical molecular events known to regulate the cell cycle and apoptotic machinery. Immunofluorescence and flow cytometric analysis of Annexin V-FITC staining provided evidence for the induction of apoptosis. PA treatment of BC cells resulted in increased activity/expression of mitochondrial cytochrome C, caspases 7, 8 and 9 with a significant increase in the Bax:Bcl-2 ratio, suggesting the involvement of a mitochondrial-dependent apoptotic pathway. Furthermore, cell cycle analysis using flow cytometry showed that PA treatment of cells resulted in G0/G1 arrest in a dose-dependent manner. Immunoblot analysis data revealed that, in MCF-7 cell lines, PA treatment resulted in the dose-dependent (i) induction of $p21^{WAF1/Cip1}$ and p27Kip1, (ii) downregulation of Cyclin dependent kinase (CDK) 4 and (iii) decrease in cyclin D1. These findings suggest that PA may be an effective therapeutic agent against BC.

A Survey on Understanding and Actual Condition of Breast-feeding of Woman at Oriental Medicine Hospital Postpartum Care Center (한방병원 산후조리센터 산모를 대상으로 한 모유수유 실태 및 인식조사)

  • Jung, Ji-Ho;Kim, Mi-Ki;Park, Jin-Soo;Shin, Kwon-Sung;Park, Eun-Jung
    • The Journal of Pediatrics of Korean Medicine
    • /
    • v.24 no.2
    • /
    • pp.13-21
    • /
    • 2010
  • at Jeonju Wonkwang University Oriental Medicine hospital postpartum care center. Results The majority of women(79.2%) had planned breast-feeding. However, only 32.1% of women currently have fed breast-feeding. 58% of mothers who did partial breast-feeding and breast-feeding currently have planned to breast-feed more than 7 months. The main reason of bottle-fed is concern of insufficient amount of breast milk(72.2%). Also the main reason of breast-feeding is good for baby's health(90%). The average score on the test of the understanding about the methods and advantages of breast-feeding was $13.98{\pm}1.92$ out of total 18.49.1% of women thought that taking herbal medicine would affect baby during breast-feeding. Also 71.7% of women thought that herbal treatments (acupuncture, herbal medicine) would be helpful when the amount of breast milk is insufficient. Conclusions This survey has showed that in spite of full breast-feeding plan, the percentage of actual breast-feeding rate is low. Timely education is needed to increase breast-feeding rate. It is worth to consider herbal treatment to increase breast milk amount. In addition to that it is necessary to create a hospital environment in which mothers can easily begin breast-feeding as soon as possible after delivery. Moreover, beyond the medical field, political and social supports for breast-feeding are needed.

Effects of Breast Massage on Breast Pain, Breast-milk Sodium, and Newborn Suckling in Early Postpartum Mothers (유방마사지가 산욕초기 어머니의 유방 통증, 유즙 소디움, 신생아 흡유 횟수에 미치는 효과)

  • Ahn, Suk-Hee;Kim, Jin-Hee;Cho, Jung-Suk
    • Journal of Korean Academy of Nursing
    • /
    • v.41 no.4
    • /
    • pp.451-459
    • /
    • 2011
  • Purpose: In this study the effects of breast massage on breast pain, breast-milk sodium, and newborn suckling in early postpartum mothers were investigated. Methods: The design was a non-synchronized nonequivalent control group pretest-post-test design. Sixty postpartum mothers who were admitted to a postpartum care center and had problems with breastfeeding were recruited. Of these mothers, 44 were assigned to the intervention group and received two 30-minute breast massages within 10 days of postpartum period. The others were assigned control group and received only routine care. Breast pain was measured using a numeric pain scale and number of times newborns suckled was observed throughout breastfeeding. Breast milk was self-collected to evaluate breast-milk sodium. Results: Mean age of postpartum mothers was 30 years old. Compared to the control group, women in the intervention group reported significant decreases in breast pain (p<.001), increases in number of times newborns suckled after the first and second massage (p<.001), and a decrease in breast-milk sodium after the first massage (p=.034). Conclusion: Breast massage may have effects on relieving breast pain, decreasing breast-milk sodium, and improving newborn suckling. Breast massage can be used to solve breast problems. Further research is needed to validate our findings.

BreastLight Apparatus Performance in Detection of Breast Masses Depends on Mass Size

  • Shiryazdi, Seyed Mostafa;Kargar, Saeed;Taheri-Nasaj, Hossein;Neamatzadeh, Hossein
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.16 no.3
    • /
    • pp.1181-1184
    • /
    • 2015
  • Background: Accurate measurement of breast mass size is fundamental for treatment planning. We evaluated performance of BreastLight apparatus in detection breast of masses with this in mind. Materials and Methods: From July 2011 to September 2013, a total of 500 women referred to mammography unit in Yazd, Iran for screening were recruited to this study. Performance of BreastLight in detection breast masses regard their sizeing, measured with clinical breast examination (CBE), mammography and sonography, was assessed. Sonographic and mammography examinations were performed according to breast density among women in two groups of women younger (n=105) and older (n=395) than 30 years. Size correlations were performed using Spearman rho analysis. Differences between mass size as assessed with the different methods (mammography, sonography, and clinical examination) and the BreastLight detection were analyzed using $X^2$-trend test. Results: Performance of the BreastLight in detection of lesions smaller than or equal to 1 cm assessed by CBE, mammography and sonography was 4.4%,7.7% and 12.5% and for masses larger than 4 cm was 65%, 100% and 57.1%, respectively. The performance of BreastLight in detection was significantly increased with larger masses (p<0.001). Conclusions: We conclude that clinical measurement of breast cancer size is as accurate as that from mammography or ultrasound. Accuracy can be improved by the use of a simple formula of both clinical and mammographic measurements.

Effects of Oketani Breast Massage on Breast Discomfort, Breast Pumping Time and Breast-milk compositions in Preterm Infants' Mothers (미숙아를 분만한 산모에게 적용한 오케타니 유방 마사지가 유방 불편감, 모유 유축시간과 모유 성분에 미치는 효과)

  • Kim, Hee-Young;Kim, Kyeong-Uoon
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.13 no.2
    • /
    • pp.701-709
    • /
    • 2012
  • The purpose of this study was to measure breast discomfort, breast pumping time, and breast milk compositions, specially lipid, calorie, and creamatocrit, after Oketani breast massage program. This study is a single group pretest-posttest design. Seventeen mothers who have preterm babies were received Oketani breast massage program. To evaluate the effects of Oketani massage program, data were measured the breast discomfort by the visual analogue scale, breast pumping time, and breast milk lipid, calorie, creamatocrit by a Creamatocrit plus machine, the standard centrifuge with digital calipers. The breast discomfort was significantly reduced after two Oketani breast massage programs(p<.05). In terms of composition of breast milk, lipid, calorie, and crematocrit were significantly increased after second Oketani massage program(p<.05). The Oketani breast massage program applied to mothers who have preterm babies could reduce breast discomfort and increase lipid, calorie, and creamatocrit of breast milk.

Development of a Breast Cancer Awareness Scale for Thai Women: Moving towards a Validated Measure

  • Rakkapao, Nitchamon;Promthet, Supannee;Moore, Malcolm A;Hurst, Cameron P
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.17 no.2
    • /
    • pp.851-856
    • /
    • 2016
  • Background: Breast cancer is a major health problem among women around the world. Recent developments in screening and treatment have greatly improved the prognosis of patients with breast cancer in developed countries. However, in developing countries breast cancer mortality remains high.Breast cancer awareness is a first and important step in reducing breast cancer mortality. The development of a validated instrument to measure breast cancer awareness is crucial for the understanding and implementation of suitable health education programs to facilitate early deletion and minimize mortality. Objective: The objective of this study was to develop an instrument for the assessment of breast cancer awareness in Thai women. Materials and Methods: This methodological study was conducted in two stages: (1) literature searches and semi-structured interviews were conducted to generate items of the breast cancer awareness scale (B-CAS) which were subsequently examined for content and face validity, and (2) an exploration of the factor structure of the resulting instrument and an examination of its reliability. Data were collected using a self-administered questionnaire in Thai women aged 20-64 in August, 2015. Results: A total of 219 women (response rate 97.4 %) participated in this validation study. The B-CAS contains five domains with 53 items on breast cancer awareness: 1) knowledge of risk factors, 2) knowledge of signs and symptoms, 3) attitude to breast cancer prevention, 4) barriers of breast screening, and 5) health behavior related to breast cancer awareness. Items with a content validity index < 0.80 were excluded, and factor structure for the remaining items reflected the hypothesized five factor model. The scales based on all retained items was shown to have strongly internal consistency reliability (Cronbach's ${\alpha}=0.86$). Conclusions: The B-CAS provides good psychometric properties to assess breast cancer awareness in women. It can be used to examine breast cancer awareness in Thai women and it could lead to the development and evaluation of suitable educational interventions for raising breast cancer awareness. Future research should focus on further validating the B-CAS including an assessment of construct and criterion-based validity.

A Study on the Usefulness of Breast Shielding Apron for Reducing Exposure Dose in Mammography (유방X선촬영 시 피폭선량 감소를 위한 유방촬영용 차폐복의 유용성에 관한 연구)

  • Koo, Bon-Yeoul;Kim, Ji-Won
    • Journal of radiological science and technology
    • /
    • v.42 no.2
    • /
    • pp.99-104
    • /
    • 2019
  • Mammography, conducted every two years, causes cancer due to regular exposure to radiation while reducing rate of death caused by breast cancer. The study evaluates the effect of breast shielding apron made to shield off scattered radiation that occurs to the breast when the opposite side breast is mammogramed. AGD was measured using ACR phantom, composed of 50% mammary glands and 50% fat, and radiation was measured before and after wearing the apron on the breast when the opposite side of the breast is mammogramed. When CC direction mammography was conducted to a breast, the AGD was 1.84 mGy. When CC direction and MLO direction mammography were done to a breast, the average dose detected from the opposite side breast from four directions(top to bottom and medial to lateral) was $140{\mu}Gy$ with maximum dose of $256{\mu}Gy$ at medial side. After putting on the apron, the dose, caused by scattered radiation, was not detected from any of the four directions. Using of breast shielding apron is expected to minimize the radiation exposure by blocking scattered radiation to the breast shielded, when mammography is done to the opposite side breast.

Brassiere Pattern Development for Augmentation Mammaplasty Patients (유방 확대수술 환자용 브래지어 패턴 개발)

  • Sohn, Boo-hyun;Yi, Kyong-Hwa
    • Journal of the Korean Society of Clothing and Textiles
    • /
    • v.41 no.4
    • /
    • pp.646-660
    • /
    • 2017
  • This study provides basic data to develop a brassiere pattern that can cover the big breast of breast enlargement patients. In this study, we also showed areas of the brassiere cup pattern and the body surface of the breast on a breast enlargement patient. The results of the study are as follows. Correlation analysis was obtained between volume and body surface area and breast detail dimensions. After the correction process, we proposed a research bra pattern for breast augmentation patients. The cup-boundary in bra patterns of breast enlargement surgery patients is longer than the bra patterns of the general breast; therefore, the height of the inner and outer edges of the upper cups is higher. Also, it is necessary to set the new breast upper point when measuring the upper length in patients with breast augmentation surgery because the points of circumference of the breast are marked higher than chest circumference.

Correlation of Breast Tissue Density and Body Mass Index (유방조직의 밀도와 체질량지수와의 상관관계)

  • Yang, Jung-Hak;Lee, Taik-Jong
    • Archives of Plastic Surgery
    • /
    • v.37 no.6
    • /
    • pp.732-735
    • /
    • 2010
  • Purpose: In performing breast reconstruction, making symmetrical breast is still a challenge. A precise estimate of the volume of the breast specimen is necessary to reconstruct a symmetrical and aesthetically pleasing breast. This study aims at finding out the relationship with breast tissue density and body mass index designed to apply for breast reconstruction. Methods: By using the Archimedes' principle, the authors calculated the volume of the breast specimen and drew a correlation between the density of breast specimen and BMI. From October 2002 to November 2004, this method was used on 197 patients to predict breast volume for TRAM flap reconstruction. Results: The mean density was 0.9954g/cc and had no correlation with BMI (p-value=0.069). There was no difference between denstiy of breast tissue after skin spared mastectomy and that of breast tissue after nipple spared mastectomy. Conclusion: These data will be helpful to predict the needed volume for breast reconstruction.

Possible role of Pax-6 in promoting breast cancer cell proliferation and tumorigenesis

  • Zong, Xiangyun;Yang, Hongjian;Yu, Yang;Zou, Dehong;Ling, Zhiqiang;He, Xiangming;Meng, Xuli
    • BMB Reports
    • /
    • v.44 no.9
    • /
    • pp.595-600
    • /
    • 2011
  • Pax 6, a member of the paired box (Pax) family, has been implicated in oncogenesis. However, its therapeutic potential has been never examined in breast cancer. To explore the role of Pax6 in breast cancer development, a lentivirus based short hairpin RNA (shRNA) delivery system was used to knockdown Pax6 expression in estrogen receptor (ER)-positive (MCF-7) and ER-negative (MDA-MB-231) breast cancer cells. Effect of Pax6 silencing on breast cancer cell proliferation and tumorigenesis was analyzed. Pax6-RNAi-lentivirus infection remarkably downregulated the expression levels of Pax6 mRNA and protein in MCF-7 and MDA-MB-231 cells. Accordingly, the cell viability, DNA synthesis, and colony formation were strongly suppressed, and the tumorigenesis in xenograft nude mice was significantly inhibited. Moreover, tumor cells were arrested at G0/G1 phase after Pax6 was knocked down. Pax6 facilitates important regulatory roles in breast cancer cell proliferation and tumor progression, and could serve as a diagnostic marker for clinical investigation.