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The Effect of Bovine Plasma for the Preparation of Mayonnaise on Quality Characteristics (Bovine Plasma의 Mayonnaise 제조 적성에 관한 연구)

  • 이진영
    • The Korean Journal of Food And Nutrition
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    • v.15 no.4
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    • pp.350-356
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    • 2002
  • This study was carried out to investigate the effect of bovine plasma for the preparation of mayonnaise on quality characteristics through the tests of viscosity, color, emulsion stability and sensory evaluation. In the case of standard mayonnaise preparation with the bovine plasma addition from 0.01% to 0.1%, The result of viscosity, color, emulsion stability and acceptability are improved. When the bovine plasma(5%) was only used to Prepare mayonnaise without e99 yolk, the result of color and acceptability were reduced, whereas viscosity, emulsion stability were observed as the characteristic of standard mayonnaise. The sample mayonnaise containing the e99 yolk(50%) and bovine Plasma (0.5~1.0%) was observed as the characteristics of standard mayonnaise without color.

Investigation of Bovine Pericardial Heterograft[III] - Experimental Evaluation of Calcification in Glutaraldehyde-preserved Bovine Pericardium - (우심낭을 이용한 이종이식 보철편의개발[III]: Glutaraldehyde에 보존한 우심낭의 석회화에 관한 실험적 연구)

  • 김기봉
    • The Korean Journal of Thoracic and Cardiovascular Surgery
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    • v.24 no.9
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    • pp.837-842
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    • 1991
  • Calcification is a major problem in glutaraldehyde-preserved bioprosthetic valves. We have used bovine pericardium processed in a solution containing 0.625% glutaraldehyde, 0.05M HEPES buffer and 0.26% magnesium chloride in saline. And, we also treated the glutaraldehyde-preserved bovine pericardium with a surfactant, Triton X - 100 to reduce calcification. To evaluate the degree of calcification. 4 kinds of pericardial xenografts, group I [Xenomedica, equine pericardial xenografts], group II [0.625% glutaraldehyde-preserved bovine pericardiums], group III [0.5% Triton X - 100 treated bovine pericardiums], and group IV [1.2% Triton X - 100 treated bovine pericardiums] were implanted in subcutaneous layer of growing rabbits, and they were explanted about 3 months later. The mean calcium contents[%/mg of dry tissue] of 0.5% and 1.2% Triton X - 100 treated bovine pericardiums [80.0$\pm$27.1%: 78.6$\pm$47.0% respectively] were lower than those of glutaraldehyde-preserved bovine pericardiums[126.2$\pm$29.8] [p=0.05]. Thus, under the conditions of subcutaneous implantation in rabbits, Triton X - 100 was efficient in calcification mitigation.

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In vitro Development of Interspecies Somatic Cell Nuclear Transfer Embryos Derived from Murine Embryonic Fibroblasts and Bovine Oocytes

  • Yun, J.I.;Koo, B.S.;Yun, S.W.;Lee, Chang-Kyu
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.11
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    • pp.1665-1672
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    • 2008
  • Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studies of iSCNT using murine cells and bovine oocytes have been reported, the development of murine-bovine iSCNT embryos beyond the 8-cell stage has not been successful. In this paper, we examined the developmental potential of embryos reconstructed with a murine embryonic fibroblast as the nuclear donor and a bovine oocyte as the cytoplasm recipient. The reconstructed embryos were cultured in CZB (murine medium) or CR1aa (bovine medium). In addition, for the development of a murine-bovine iSCNT blastocyst, the antioxidant ${\beta}$-mercaptoethanol (${\beta}ME$) was supplemented to CR1aa medium. Furthermore, to verify the mouse genome activation in murine-bovine iSCNT embryos, RT-PCR analysis of murine Xist was performed. The development of the murine-bovine iSCNT embryos cultured in CR1aa was significantly higher than that in CZB (p<0.05). With respect to the effect of BME on the development of the murine-bovine iSCNT blastocyst, addition of BME produced a significant increase in blastocyst development (p<0.05). Karyotype analysis confirmed that the reconstructed embryos were derived from murine cells (40XX). The Xist gene was gradually increased from the 8-cell stage to the blastocyst stage. This is the first report of blastocyst development of iSCNT embryos derived from murine somatic cells and bovine oocytes. These results demonstrate that bovine cytoplasm can support the development of later stages of a preimplantation embryo from murine-bovine iSCNT.

An Observation on the Outbreak Patterns of Zoonoses in Chonnam Area (인축공통 전염병의 발생양상에 관한 고찰 -전남지역을 중심으로-)

  • 정희곤
    • Journal of Environmental Health Sciences
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    • v.15 no.2
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    • pp.117-121
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    • 1989
  • An observation on the outbreak patterns of zoonoses was carried out on 261,862 cattle, 1,967 swine, 91,500 fowl samples in Chronnam Province. And it was classified by ppsitive.reaction from numbers of request examined and diagnosis to Animal Health Center of Chonnam Province from 1983 to 1988. The results are summarized as follows: Incidence rate of zoonoses in Chonnam area was observed in the order of alveolar sarcoma of fowl (75.0%), bovine facioliasis (47.6%), bovine mastitis (19.0%), bovine salmonellosis (12.6%), salmonellosis of swine (11.0%), salmonellosis of fowl (8.9%), bovine streptogenes (6.7%), tetanus of bovine (2.0%), streptogenes of swine (1.7%), pullorum disease (0.6%), bovine tubercullosis (0.02%), and bovine brucellosis (0.01%). And, especially bovine facioliasis was observed highest outbreaks (89.4%).

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Effects of bovine antisperm antibodies on fertilizing capacity of bovine spermatozoa (소 항정자항체가 소 정자의 수태능력에 미치는 영향)

  • Kim, Kye-seong;Roh, Sang-ho;Lee, Kang-nam;Lee, Byeong-chun;Hwang, Woo-suk
    • Korean Journal of Veterinary Research
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    • v.37 no.4
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    • pp.925-934
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    • 1997
  • This study was directed at inducing the production of antibodies by immunizing heifers with bovine sperm antigen and on measuring the serum antibodies using indirect immunofluorescence assay(IFA) and agglutination test. The effect of antisperm antibodies on fertilizing capacity of bovine spermatozoa was evaluated. 1. Three heifers between 12- and 15- month old were immunized with bovine spermatozoa or phosphate-buffered saline. In heifers immunized with bovine spermatozoa serum IgG level was highest between 3 weeks and 5 weeks postimmunization detected by IFA. The antibody levels persisted through week 7 and slowly declined until week 20 and then antisperm antibodies were localized on spermatozoa. The fluorescent antisperm antibodies were detected at 2~20 weeks and at 6~9 weeks postinoculation on acrosome and tail, respectively. Among 21 sera from repeat breeder cows, only one cow has shown positive antisperm antibody response detected by IFA. 2. In spite of vital rate of bovine sperm after swim-up was not significantly affected by different concentration of antisperm antibodies in sera, the numbers of bovine sperm after swim-up were significantly reduced in proportion to the increased concentration of antibodies. Above 1/512 dilution of antibody neither influence on vital rate and numbers of bovine sperm nor sperm agglutination after swim-up. The study has also shown that the vital rate and number of sperm after swim-up and capacitation were also significantly reduced by the addition of antisperm antibodies. Although antisperm antibodies did not influence on the acrosome reaction rate of sperm during swim-up, did significantly reduce the sperm acrosome reaction rate after capacitation. The studies have resulted that the bovine antisperm antibodies can prevent the sperm motility by agglutination and block the capacitation and acrosome reaction of bovine sperm.

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Investigation of Bovine Pericardial Heterograft (II0) : Clinical applications of glutaraldehyde-preserved bovine pericardium (우심낭을 이용한 이종이식 보철편의 개발 (II) - 0.625% Glutaraldehyde 에 보존한 우심낭의 임상 적용 -)

  • 김기봉
    • The Korean Journal of Thoracic and Cardiovascular Surgery
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    • v.23 no.3
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    • pp.465-473
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    • 1990
  • Glutaraldehyde have been used as the most effective cross-linking agent for stabilizing collagen fibers and preventing biodegradation. We processed bovine pericardium in a solution containing 0.625% glutaraldehyde,0.05M HEPES buffer and 0.26% magnesium chloride in saline. The glutaraldehyde-preserved bovine pericardium was implanted in 36 patients at Seoul National University Hospital during a 11-month period between May 1989 and March 1990. 24 were males and 12 females, with ages ranging from 6 months to 168 months [mean age of 43 months]. In 12 patients, the glutaraldehyde-preserved bovine pericardium was used for orthotopic reconstruction of the pericardial sac. In 24 patients. the glutaraldehyde-preserved bovine pericardium was heterotopically implanted.; pulmonary monocusp implant and RVQT [right ventricular outflow tract] patch widening were performed in 10 patients, pulmonary monocusp implant in 6, RVOT patch widening in 4, valved conduit in 2, conduit and pulmonary angioplasty in 1, and ventricular septation in l. With vascular suture techniques, the anastomoses were immediately tight. There was no bleeding from the needle holes and no oozing through bovine pericardium itself. During the follow-up period of up to 10 months, no infections of the glutaraldehyde-preserved bovine pericardium occurred and no bovine pericardium-related complications were observed in this series.

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Study on Freezability of In Vitro-Matured Bovine Follicular Oocytes (체외성숙 소 난포란의 동결성에 관한 연구)

  • Kim, S.;Chung, Y. C.;Kim, C. K.;Yoon, J. T.;Lee, J. W.
    • Journal of Embryo Transfer
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    • v.10 no.3
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    • pp.219-227
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    • 1995
  • This study was investigated to test in vitro-maturation rate of bovine follicular oocytes freezability of in vitro-matured bovine follicular oocytes with different stock solution in Glycerol and Propanediol, freezability of in vitro-rnatured bovine follicular oocytes on cryoprotectants, the viability of in vitro-rnatured bovine follicular oocytes by morphologically normal and FDA staining method. 1. The maturation rates of bovine follicular oocytes classified as grade A, B and C was 88, 63 and 21%, respectively. 2. Freezability of in vitro-matured bovine follicular oocytes on stock solution, TCM-199+5% FCS and m-PBS + 5% FCS was 61%(n=105), 48%(n=62) in $_1$M Glycerol and freeability of in vitro-matured bovine follicular oocytes on stock solution, TCM-199 +5% FCS and m-PBS + 5% FCS was 68%(n=112), 42%(n=57) in 1~2 Propanediol. The results indicate that freezability of in vitro-matured bovine follicular oocytes with different stock solution is important. 3. Freezability of in vitro-matured bovine follicular oocytes on cryoprotectants was Glycerol and PROH was 56%(n=167), 57%(n=169). The results indicate that PROH was superior to Glycerol. 4. The rates of morphologically normal IVM oocytes after thawing of cryopreserved oocytes with Glycerol and PROH were 39%(n=$_1$8), 65%(n=39), respectively. The results indicate that PROH was superior to Glycerol. 5. The fluorescent light intensity after thawing of cryopreserved oocytes classified with Positive, Partial-I, Partial-II, Negative with Glycerol and PROH. The results of FDA-positive 24%, 42%, Partial-I 17%, 10%, Partial- H 20%, 12%, FDA-negative 39%, 37%, and Partial-I, II, respectively.

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Epidemiological survey of Bovine Tuberculosis in Kyonggi province (1987-1996) (경기지역의 우결핵 발생에 관한 역학적 고찰(1987-1996))

  • 국정희;심항섭;고태오;우종태;조중현;박유순
    • Korean Journal of Veterinary Service
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    • v.21 no.1
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    • pp.1-12
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    • 1998
  • As the result of epidemiological analysis on bovine tuberculosis in Kyonggi province during the last ten years(1987-1996), 1. The annual incidence number of bovine tuberculosis positive farms in Kyonggi province fell down from 81 in 1987 to 14 in 1989. But the incidence is increased since 1990, in 1995 the incidence number was 102 farms of the most incidence. Total number of bovine tuberculosis positive farms were 456 farms(56.7%) in Kyonggi province during the 1987-1996 period. 2. The developmental trends of bovine tuberculosis positive heads was similiar to that of positive farms. Since 1993, the incidence was suddenly increased. Total number of bovine tuberculosis positive heads were 1,015 head(64.3%) in Kyonggi province during the 10 years. 3. Average incidence for rate during the last ten years(1987-1996) was 0.10%. The positive rate was suddenly increased since 1993 and in 1996, the positive rate was 0.23% of the highest incidence. 4. Regional Incidence number of bovine tuberculosis positive heads in Kyonggi province during the last seven years(1990-1996) was the most in Hwasung, Pochon, Pyungtek in order of Incidence and bovine tuberculosis positive density was 1.297% of the highest in Buchon. 5. In 244 farms(82.4%), bovine tuberculosis first occurred during the last seven years (1990-1996) but in farms over second occurrence, the number of bovine tuberculosis positive heads were 520 heads(58.2%).

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Effects of Glucose on the Cleavage and Further Development of Early Bovine Embryos (Glucose가 소 초기배의 분할 및 발육에 미치는 영향)

  • 노상호;이병천;황우석
    • Journal of Embryo Transfer
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    • v.12 no.2
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    • pp.161-169
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    • 1997
  • This study was conducted to compare the insemination time of bovine oocytes and determine the effects of glucose(1.5 mM) on the development of bovine embryos at early cleavage stage. Oocytes were matured for 24 h, followed by exposure to sperm and cultured in modified Tyrode's media drops or with bovine oviduct epithelial cell monolayer prepared in TCM199(BOECM). Insemination time and culture system were varied in each experiment. In experiment 1, to investigate the developmental capacity of bovine embryos after different time of exposure to sperm, bovine ova and sperm were co-incubated for 18, 30 or 54 h, respectively. The development to blastocysts of 30 and 54 h insemination groups were significantly higher(P<0.05) than 18 h group, and in case of blastocysts of cleaved embryos, 30 h group were significantly higher(P<0.05) than other groups. In experiment 2, we investigated the effect of glucose on early bovine embryos. After 18 h insemination, in vitro fertilized oocytes were separated following 3 groups ; G+0, C+24 and C+48. Oocytes of G+0 group were cultured in glucose added Tyrode's medium after fertilization, oocytes in C+24 and C+48 groups were cultured in glucose free Tyrode's medium after fertilization. After 24 h culture, G+24 group was moved to glucose added medium. All oocytes of 3 groups were moved to BOECM after 48 h culture. The rates of cleavage and development to blastocysts in G+0 group were significantly lower than other groups. In experiment 3, we determined the effects of glucose exposure from 8 to 20 h after insemination on the cleavage and development of oocytes. The oocytes in glucose added group had high capacity of cleavage and further development. This study shows that in bovine oocytes, the optimal exposure to sperm is 30 h and glucose exposure to bovine one-cell embryos is detrimental to their first cleavage and further development in vitro but there has no evidence of detrimental effect of glucose(1.5 mM) exposure to bovine embryos over the two-cell stage in vitro.

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Effects of Heat-treated Bovine Lactoferrin on the Growth of Lactococcus lactis subsp. cremoris JCM 20076

  • Kim, Woan-Sub
    • Journal of Dairy Science and Biotechnology
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    • v.37 no.2
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    • pp.129-135
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    • 2019
  • This study investigated the effects of heat-treated and non-heat-treated bovine lactoferrin on the growth of Lactococcus lactis subsp. cremoris JCM 20076. The addition of heat-treated and non-heat-treated bovine lactoferrin in adjusted MRS medium stimulated the growth of Lc. cremoris JCM 20076. Heat-treated bovine lactoferrin had a greater impact on the growth of Lc. cremoris JCM 20076 compared to that with non-heat-treated bovine lactoferrin. Bovine lactoferrin heated at $65^{\circ}C$ for 30 min stimulated the growth of the bacteria more than that heated at $80^{\circ}C$ for 5 min. Furthermore, the growth of Lc. cremoris JCM 20076 increased substantially with heat-treated bovine lactoferrin at a concentration of 1 mg/mL.