• Title/Summary/Keyword: Boar

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Effects of Pyruvate and Taurine for In Vitro Preservation in Boar Semen and the Developmental Rates of Embryos Fertilized by Semen Treated with Antioxidant

  • Jang H. Y.;Cheong H. T.;Kim C. I.;Park C. K.;Yang B. K.
    • Reproductive and Developmental Biology
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    • v.29 no.2
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    • pp.133-139
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    • 2005
  • Oxidative stress is one of the major causes of failure in in vitro storage of boar semen. Reactive oxygen species (ROS) are known to be important mediators of such stress. The present study examined the effects of pyruvate and taurine on sperm motility and expression of BAD, Cytochrome c, Caspase-3 and Cox-2 protein in in vitro storage of boar semen, and tested the effect of semen treated with antioxidant with or without hydrogen peroxide on the development of IVM/IVF porcine embryos. Semen samples were transported to the laboratory at $17^{\circ}C$ within 2 hr after collection and were treated with different concentration of pyruvate $(1\~10mM)$ and taurine $(25\~100mM)$ with or without 250uM $H_2O_2$ respectively. The supplementation of pyruvate and taurine increased sperm motility in boar semen during in vitro incubation at $37^{\circ}C$. Expression of apoptosis protein (BAD, cytochrome c, caspase-3 and cox-2) were reduced in the group of boar semen treated with pyruvate and taurine when compared to the other groups. The developmental rates of IVM/IVF porcine embryos fertilized by semen treated with pyruvate and taurine were significantly increased when compared to control (P<0.005). These results indicate that supplementation of pyruvate and taurine as antioxidants in boar semen extender can improve the semen quality and increase in vitro development of porcine IVM/IVF embryos when boar semen treated with antioxidants was used for in vitro fertilization.

Diet of The Wild boar (Sus scrofa) in Agricultural Land of Geochang, Gyeongnam Province, Korea (경남 거창 농경지 멧돼지(Sus scrofa)의 식이물 분석)

  • Lee, Seong-Min;Lee, Woo-Shin
    • Journal of Korean Society of Forest Science
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    • v.103 no.2
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    • pp.307-312
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    • 2014
  • Wild boar have increased in recent years in Korea where damage to agricultural crops is considered as a social issue. The ecological studies on wild boar are essential for the effective management in the future. During May and December 2012, We collected 79 wild boar stomach samples from culling program implementation to reduce agricultural damage in Geochang County and analyzed stomach contents to figure out wild boar diet habit and which crop was preferred. There was seasonal variation in the diet. Plant foods represented up to 93.5% of the diet and were consumed more frequently than animal foods. Invertebrates occurred with high frequency but were underestimated in dry weight because they are rapidly digested. Agricultural crops consumed comprised mainly chestnuts(33%), rice(33%), and apples(24%). Agricultural crops are also a potentially important food resources for wild boar. The frequency of occurrence of earthworms was 50% of diet. Earthworm consumption generally increased in summer, and then decreased in winter. Our results show that wild boar can be considered an omnivore whose diet consists of plant and animal foods. To reduce wild boar agricultural damages, We have to focus on the prevention effort to rice paddy adjacent to chestnut forest and to apple orchard.

Prevalence of Emergency Traumatic Injuries in 100 Wild Boar Hunting Dogs (100마리의 야생 멧돼지 사냥견에서 응급 외상성 질환의 분류)

  • Ko, Jae-Jin;Kim, Se-Hoon;Ji, Joong-Ryong;Shim, Kwan-Seob;Kim, Nam-Soo
    • Journal of Veterinary Clinics
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    • v.27 no.6
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    • pp.718-722
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    • 2010
  • To investigate patterns and severity of hunting-related emergency traumatic injuries in wild boar hunting dogs. One hundred wild boar hunting dogs with emergency traumatic injuries sustained during wild boar hunting as a result of wild boar attack and accidental shooting of ensnarement in a trap. The retrospective study involved 100 dogs brought to CAMC for treatment of emergency traumatic injury sustained during wild boar hunting in Jeon-buk province from August 2007 to April 2008. Medical information obtained from the medical records included signalment; cause of injury; number, location and severity of injuries; and mortality. The 100 patients displayed 136 injuries (single injury in 71 dogs and multiple injuries in 29 dogs). Causes of the emergency traumatic injuries were wild boar attack (n = 92), accidental shooting (n = 7), and entrapment (n = 1). The thoracic area was the most common site of injury. The most common injury severity score (ISS) was code 2. The mortality rate was 9%, and all deaths involved thoracic injury. Emergency traumatic injuries sustained during wild boar hunting are most commonly thoracic injuries caused by prey attack. The nature of the injuries can differ from those typically encountered by small animal veterinarians.

Ameliorative Effect of Chitosan Complex on Miniature Pig Sperm Cryopreservation

  • Hong, Hye-Min;Sim, Ga-Young;Park, So-Mi;Lee, Eun-Joo;Kim, Dae-Young
    • Journal of Embryo Transfer
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    • v.33 no.4
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    • pp.337-342
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    • 2018
  • Cryopreservation is mainly used for preservation of boar sperm. However, this method stresses the sperm by reactive oxygen species (ROS), and the conception rate and the litter size are not more efficient than the liquid preservation of spermatozoa. Therefore, we use chitosan which is a natural product derived antioxidant compound. We used GnHA (chitosan+hyaluronic acid) and GnHG (chitosan hydrogel) as chitosan complexes to cryopreserve boar sperm for improve sperm metabolism and function. Sperm parameter (sperm motility, progressive motility, path velocity, straight-line velocity, curvilinear velocity) is measured by computer-assisted sperm analysis (CASA) using frozen sperm with GnHA or GnHG (0, 0.25, 0.5, 1 mg/mL), respectively. Also, lipid peroxidation analysis using malondialdehyde (MDA) is performed to confirm the antioxidative effect of chitosan in frozen spermatozoa. CASA analysis showed GnHA and GnHG are effective against cryopreserved boar sperm. And antioxidant effect is measured by lipid peroxidation analysis. GnHA and GnHG, which is chitosan complex are effective for boar sperm cryopreservation by antioxidant effect.

Estimates of Genetic Correlations between Production and Semen Traits in Boar

  • Oh, S.H.;See, M.T.;Long, T.E.;Galvin, J.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.2
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    • pp.160-164
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    • 2006
  • Currently, boars selected for commercial use as AI sires are evaluated on grow-finish performance and carcass characteristics. If AI sires were also evaluated and selected on semen production, it may be possible to reduce the number of boars required to service sows, thereby improving the productivity and profitability of the boar stud. The objective of this study was to estimate genetic correlations between production and semen traits in the boar: average daily gain (ADG), backfat thickness (BF) and muscle depth (MD) as production traits, and total sperm cells (TSC), total concentration (TC), volume collected (SV), number of extended doses (ND), and acceptance rate of ejaculates (AR) as semen traits. Semen collection records and performance data for 843 boars and two generations of pedigree data were provided by Smithfield Premium Genetics. Backfat thickness and MD were measured by real-time ultrasound. Genetic parameters were estimated from five four-trait and one five-trait animal models using MTDFREML. Average heritability estimates were 0.39 for ADG, 0.32 for BF, 0.15 for MD, and repeatability estimates were 0.38 for SV, 0.37 for TSC, 0.09 for TC, 0.39 for ND, and 0.16 for AR. Semen traits showed a strong negative genetic correlation with MD and positive genetic correlation with BF. Genetic correlations between semen traits and ADG were low. Therefore, current AI boar selection practices may be having a detrimental effect on semen production.

Phelligridin D from Phellinus baumii Reduces Boar Sperm Viability

  • Yi, Young-Joo;Lee, In-Kyoung;Seo, Dong-Won;Yun, Bong-Sik;Lee, Sang-Myeong
    • The Korean Journal of Mycology
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    • v.44 no.2
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    • pp.122-125
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    • 2016
  • Phelligridin D (Phe D) is a compound isolated from Phellinus baumii, which is known for various biological activities. In this study, the authors examined the effect of Phe D on boar spermatozoa for its potential application in assisted reproductive technology for mammals. Sperm motility and deubiquitinylating activity significantly decreased when boar spermatozoa were incubated with Phe D (>$0.5{\mu}M$). The fluorescence intensities of dead sperm, and reactive oxygen species production increased after sperm incubation in the presence of Phe D. Although Phe D is associated with antioxidant and free radical scavenging activity, sperm viability deteriorated after its addition. This could lead to fertilization failure, including that following artificial insemination or in vitro fertilization. Phe D might have other biological functions in spermatozoa, and therefore requires additional studies in the future.

Spargana in a Weasel, Mustela sibirica manchurica, and a Wild Boar, Sus scrofa, from Gangwon-do, Korea

  • Lee, Seung-Ha;Choe, Eun-Yoon;Shin, Hyun-Duk;Seo, Min
    • The Korean Journal of Parasitology
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    • v.51 no.3
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    • pp.379-381
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    • 2013
  • To know the status of sparganum (plerocercoid of Spirometra erinacei) infection in the Korean wild life, several species of wild animals were captured in Gangwon-do and examined for their status of infection with spargana. From February to December 2011, a total of 62 wild boars, 5 badgers, 1 weasel, 1 Siberian chipmunk, and 53 wild rodents were captured, and their whole muscles were examined with naked eyes for the presence of spargana worms. From the weasel and 1 wild boar, a total of 5 spargana specimens were extracted. The weasel was for the first time recorded as an intermediate or paratenic/transport host of S. erinacei in Korea, and both the weasel (Mustela sibirica manchurica) and wild boar (Sus scrofa) were added to the list of wild animals carrying spargana.

Prevalence of antibodies against hepatitis E virus in wild boars (Sus scrofa) in Korea

  • Park, Yu-Ri;Park, Ji-Young;Kang, Dae-Young;Han, Do-Hyun;Yoon, Hachung;Jeong, Wooseog;An, Dong-Jun;Yeo, Sang-Geon;Park, Choi-Kyu
    • Korean Journal of Veterinary Service
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    • v.39 no.4
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    • pp.267-270
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    • 2016
  • We determined the nationwide seroprevalence of hepatitis E virus (HEV) infection in the wild boar population in Korea. Enzyme-linked immunosorbent assay (ELISA) results showed that 42% of the 528 wild boars that were hunted between 2013 and 2014 were anti-HEV antibody positive. Furthermore, all Korean provinces showed an HEV seroprevalence between 9.8% and 51.1%, suggesting that wild boar HEV infection occurs throughout the country. Importantly, infected wild boar could act as a potential reservoir for HEV and could aid transmission to other animals and humans.

Effects of α-Linolenic Acid and Bovine Serum Albumin on Frozen-thawed Boar Sperm Quality during Cryopreservation

  • Lee, Won-Hee;Hwangbo, Yong;Lee, Sang-Hee;Cheong, Hee-Tae;Yang, Boo-Keun;Park, Choon-Keun
    • Reproductive and Developmental Biology
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    • v.40 no.4
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    • pp.33-37
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    • 2016
  • This study was conducted to evaluate effect of ${\alpha}$-linolenic acid (ALA) and bovine serum albumin (BSA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryopreserved using freezing extender containing 3 ng/mL ALA and/or $20\;{\mu}g/mL$ BSA. Cryo-preserved boar sperms were thawed in $37^{\circ}C$ water-bath for 45 sec to analysis. Viability, acrosome reaction, and mitochondrial intact were analyzed using flow cytometry. In results, viability of frozen-thawed boar sperm was significantly higher in only ALA+BSA supplement group than control group (p<0.05), whereas there was no difference either in ALA or BSA supplement. However, acrosome reacted sperm in both of live and all sperm population were significantly decreased in all treatment groups than control (p<0.05). Interestingly, mitochondrial intact of boar sperm was enhanced in ALA and ALA+BSA groups compared with control (p<0.05). In this study, we showed that supplementation of ALA and BSA in freezing extender enhanced the sperm viability, mitochondrial intact and decrease acrosomal membrane damage. In conclusion, our findings suggest that quality of frozen-thawed sperm in mammalians could improve by using of ALA and BSA.

Effect of L-carnitine on sperm quality during liquid storage of boar semen

  • Yang, Kang;Wang, Na;Guo, Hai-Tao;Wang, Jing-Ran;Sun, Huan-Huan;Sun, Liang-Zhen;Yue, Shun-Li;Zhou, Jia-Bo
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.11
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    • pp.1763-1769
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    • 2020
  • Objective: This study was conducted to investigate the effect of L-carnitine on the pig semen characteristics during storage. Methods: Spermatozoa samples were examined for spermatozoa quality and then randomly divided into 5 groups: 0 (control), 12.5, 25, 50, and 100 mM L-carnitine. Sperm motility, plasma membrane integrity and antioxidant parameters (total reactive oxygen species, total antioxidant capacity, and malondialdehyde) were evaluated after 0, 3, 5, and 10 day cooled-storage at 17℃. Moreover, ATP content, mitochondria activity as well as sperm-binding and in vitro fertilizing ability of preserved boar sperm were also investigated. Results: Supplementation with 50 mM L-carnitine could effectively maintain boar sperm quality parameters such as sperm motility and membrane integrity. Besides, we found that L-carnitine had positive effects on boar sperm quality mainly through improving antioxidant capacities and enhancing ATP content and mitochondria activity. Interestingly, by assessing the effect of L-carnitine on sperm fertility and developmental potential, we discovered that the extender containing L-carnitine could improve sperm quality and increase the number of sperms bounding to zona pellucida, without improving in vitro fertility and development potential. Conclusion: These findings suggested that the proper addition of L-carnitine to the semen extender improved boar sperm quality during liquid storage at 17℃.