• Title, Summary, Keyword: Boar

Search Result 303, Processing Time 0.039 seconds

The Reduction of Hydrogen Peroxide in Viable Boar Sperm Cryopreserved in the Presence of Catalase (Catalase 첨가에 따른 돼지 정액 동결 및 융해 후 생존 정자에서 Hydrogen Peroxide의 감소)

  • Kim, Su-Hee;Lee, Young-Jun;Kang, Tae-Woon;Kim, Yong-Jun
    • Journal of Veterinary Clinics
    • /
    • v.28 no.1
    • /
    • pp.13-19
    • /
    • 2011
  • Semen cryopreservation induces the formation of reactive oxygen species (ROS), and the ROS cause sperm damage. We aimed to investigate the effects of the antioxidative enzyme catalase (CAT) on sperm quality and ROS during cryopreservation. Sperm rich fractions collected from five Duroc boars were cryopreserved in freezing extender with (200 or 400 U/mL) or without CAT (control). After thawing, sperm motility, viability, normal morphology, plasma membrane integrity, mitochondrial function and intracellular ROS were evaluated. CAT significantly improved total sperm motility at a concentration of 400 U/mL (P < 0.05), but didn't improve progressive sperm motility, viability, morphological defects, plasma membrane integrity and mitochondrial function in frozen-thawed boar sperm. In evaluation of ROS, CAT had no effect on reduction in ${\cdot}O_2$, but scavenged $H_2O_2$ in viable frozen-thawed boar sperm at concentrations of 200 and 400 U/mL (P < 0.05). In conclusion, CAT was not enough to improve quality of frozen-thawed sperm, but can reduce $H_2O_2$ generation in viable boar sperm during cryopreservation.

A Study on Efficiency of Boar Semen Extender KpA for Swine AI Aspects of Storage Temperature and pH Change (돼지 인공수정용 국산 희석액 KpA 의 효용성에 관한 연구 : 보존온도와 pH 변화 측면)

  • 정구민;김선의;신영수;김인철;이장희;임경순
    • Korean Journal of Animal Reproduction
    • /
    • v.23 no.3
    • /
    • pp.257-262
    • /
    • 1999
  • This study was carried out to evaluate the effect of pH and storage temperature on the viability of boar sperm diluted with extender KpA for swine artificial insemination. The results obtained in this experiment were summarized as follows : 1. The viability of boar sperm diluted with KpA was higher than that with BTS during storage and especially more than 60% of sperm viability in KpA was maintained through 6 days. The pH values of all extenders were kept during storage of semens following dilution. 2. The sperm diluted with acidic (pH 6.3~6.8) or alkalic (pH 7.8~8.0) KpA and stored at 4$^{\circ}C$ or 37$^{\circ}C$ were more sensitive in viability than that with neutral pH (6.8~7.3) and at 17$^{\circ}C$ storage. But pH values of all conditions were not increased rapidly. Especially acidic and alkalic diluents were more stable in pH after dilution. Conclusively, extender pH and storage temperature was the important factors for sperm viability. The KpA setting up around neutral pH was the optimal boar semen extender for maintaining liquid semen at 17$^{\circ}C$.

  • PDF

Effects of Feeding Mugwort Pelleted Diet on the Meat Quality in Pigs (쑥 펠렛사료 급여가 돼지의 육질에 미치는 영향)

  • Kim Byung-Ki;Kim Young-Jik;Kim Soo-Min
    • Food Science of Animal Resources
    • /
    • v.24 no.4
    • /
    • pp.393-398
    • /
    • 2004
  • To investigate the effect of dietary mugwort pellet on the growing performance and meat quality barrow (T1) and boar (T2) were alloted into six treatments : 1) commercial feed, 2) T1-1 and T2-1 (commercial feed supplemented with 3.0% mugwort pellet), 3) T1-2 and T2-2 (commercial feed supplemented with 5.0% mugwort pellet). They were fed experimental diets for 60 days before slaughtered. Meat samples were taken in wrap package and stored at 4$\pm$1$^{\circ}C$. Daily gain in both groups (T1 and T2) were higher than those of the control (p<0.05). Feed conversion tended to be lower in barrow group than boar group and tended to be decreased according to supplementation of mugwort pelleted diet in barrow group. In both barrow and boar groups, proximate compositions of pork were not sigificantly different, except for crude fat. Crude fat content was tended to be low in barrow when fed mugwort pelleted diet. Shear force value and sensory properties were siginificantly higher in barrow group than in boar group (p<0.05) and these results were also seemed due to great fed mugwort pelleted aiet(p<0.05). In the barrow group, values of tenderness and flavor were the highest in pigs fed diet supplemented with 3.0% mugwort. The catechin content of pork tended to be higher in boar group than in barrow group and catechin tended to increase with supplementation of mugwort in the diet.

Carcass Yields and Meat Quality by Live Weight of Korean Native Black Pigs (재래돼지의 출하체중별 도체수율 및 육질특성)

  • Cho, S.H.;Park, B.Y.;Kim, J.H.;Kim, M.J.;Seong, P.N.;Kim, Y.J.;Kim, D.H.;Ahn, C.N.
    • Journal of Animal Science and Technology
    • /
    • v.49 no.4
    • /
    • pp.523-530
    • /
    • 2007
  • A total of 127 Korean native black pigs(gilt 30, boar 97) were used to investigate the carcass properties, yields of retail cut and meat quality. The average live weight, chilled carcass percentage and retail cut percentage used in this study were 74.69kg, 71.14% and 61.09% for boar, and 75.5kg, 73.88% and 56.25% for gilt, respectively. Although gilts were higher in live weight and chilled carcass percentage whereas lower in retail cut percentage when compared to boars, there was no significantly difference between gender(p<0.05). Gilts had higher fat contents than boars regardless of weight range groups. Protein contents increased as slaughter weight increased for gilt and boar groups. Gilts contained high intramuscular fat contents had significantly lower Warner-Bratzler shear force(WBS) and water holding capacity(WHC) when compared to boar in the same weight range group(p<0.05). There was no significantly different in meat color(p>0.05) although gilts had higher L(lightness), a(redness) and b(yellowness) values when compared to the same group of boar. From the result of this study can be used for the grading guideline and quality evaluation standard of Korean native black pigs by gender and live weight.

Comparison of Extraction Methods of Chondroitin Sulfate from Meat By-products (식육부산물에서의 Chondroitin Sulfate 추출방법간의 비교)

  • Lim, D.G.;Oh, D.H.;Seol, K.H.;Lee, Moo-Ha
    • Journal of Animal Science and Technology
    • /
    • v.49 no.4
    • /
    • pp.531-538
    • /
    • 2007
  • A total of 127 Korean native black pigs(gilt 30, boar 97) were used to investigate the carcass properties, yields of retail cut and meat quality. The average live weight, chilled carcass percentage and retail cut percentage used in this study were 74.69kg, 71.14% and 61.09% for boar, and 75.5kg, 73.88% and 56.25% for gilt, respectively. Although gilts were higher in live weight and chilled carcass percentage whereas lower in retail cut percentage when compared to boars, there was no significantly difference between gender(p<0.05). Gilts had higher fat contents than boars regardless of weight range groups. Protein contents increased as slaughter weight increased for gilt and boar groups. Gilts contained high intramuscular fat contents had significantly lower Warner-Bratzler shear force(WBS) and water holding capacity(WHC) when compared to boar in the same weight range group(p<0.05). There was no significantly different in meat color(p>0.05) although gilts had higher L(lightness), a(redness) and b(yellowness) values when compared to the same group of boar. From the result of this study can be used for the grading guideline and quality evaluation standard of Korean native black pigs by gender and live weight.

Selection of the Rubbing Trees by Wild Boar (Sus scrofa) and its Ecological Role in a Mixed Forest, Korea (혼효림에서의 멧돼지(Sus scrofa) 비빔목 선택과 생태적 역할)

  • Lee, Seong-Min;Lee, Woo-Shin
    • Journal of Korean Society of Forest Science
    • /
    • v.103 no.3
    • /
    • pp.510-518
    • /
    • 2014
  • Conflicts between humans and wild boars (Sus scrofa) have increased because of causing crop damage and appearing in downtown. Management to reduce population size has mostly been implemented on pests, but knowledges about ecological values are very rare. Rubbing trees by wild boar was investigated to figure out characteristics and for management ecologically from July to October 2013 in a mixed forest, Geochang, Gyeongnam Province, Korea. Pinus rigida was most frequently founded, and rubbing trees, both conifers and deciduous, were mainly located in 200~600 m above sea level. DBH was no significant difference between rubbing and control trees. The use intensity of conifers was higher than that of deciduous. The coverage of foliage layers and the number of woody plants within a radius of 2.5 m of trees (rubbing and control) were also investigated and compared to determine ecological characteristics. There were 1,332 individuals founded from 25 families. In the coverage of foliage layers, the understory and midstory showed statistically significant, but not in the subover and overstory. The number of Shrubs and saplings are higher in rubbing trees than those of control, but no difference in stems. Total individuals, the number of species, species diversity index(H') within a radius 2.5 m is considerably higher in rubbing trees with high intensity than those of low intensity and control. Our results show that wild boar mainly selected and used conifers more frequently as rubbing trees. Wild boar also modified the physical environments around rubbing trees. Consequently, the positive effects such as species diversity is increase. Thus the conservation policies is required after the intensive population reduction every 3-4 years for forest ecological management rather than annual sustainable hunting.

Secondary Dispersion of Several Broadleaved Tree Seeds by Wildlife in Mt. Jungwang, Pyeongchang-gun, Korea (중왕산 몇 활엽수 종자의 야생동물에 의한 2차 분산)

  • Kim, Gab-Tae;Kim, Hoi-Jin
    • Korean Journal of Environment and Ecology
    • /
    • v.27 no.1
    • /
    • pp.64-70
    • /
    • 2013
  • To examine the proportion of secondary seed dispersion by wildlife and to identify the related wildlife, We measured seed removal rates and related wildlife at the natural mixed broadleaved forest in mountain Jungwang, Pyeungchang-gun in middle part of Korean peninsula, seed-tray contained four kind of tree seeds were setted from mid-september to late-October for three years. Mean seed removal rates(MSRR) of acorn(Q. mongolica)is shown the highest values 87.2%, next MSRR of samara(A. pseudosieboldianum) does 33.1%, MSRR of samara(A. pictum subsp. mono) does 29.2%. and then SRR of samara(A. mandshuricum) does only 13.6%. Acorns are removed more faster than samaras. Among the related wildlife, Siberian chipmunks most freqently visit seed tray, and ordered Korean squirrel, Wild boar, and Yellow-throated marten. Most secondary dispersed acorns might be consumed by Wild boar, and small portions does comsumed or scatter-hoarded by rodents. Most secondary dispersed samaras might be consumed by rodents, and small portions does scatter-hoarded by rodents. A few sound seeds are remained on the forest floor in late October owing to the seed consunption and seed hoarding by wildlife. We observe seedling establishment of four broadleaved tree species. From these results, We proved Wild boar is seed comsummer, and rodents are seed consummer and seed disperser. Further studies on secondary seed dispersion, seed consumption and hoarding and related wildlife should be needed.

The Effects of Cryoprotectants on Motility and Viability Kinetics of Liquid Boar Semen at $4^{\circ}C$ (액상 보존액 내 동결 보호제가 $4^{\circ}C$에 보관된 액상 돼지 정액의 운동성과 생존성에 미치는 효과)

  • Jeon, Yu-Byeol;Park, Kwang-Woo;Kwak, Seong-Sung;Jeong, Seung-A;Yoon, Jun-Chul;Hyun, Sang-Hwan
    • Journal of Embryo Transfer
    • /
    • v.27 no.1
    • /
    • pp.51-55
    • /
    • 2012
  • The objective of this study was to investigate the motility and kinematics of boar sperm that while stored at 4C. The samples of fresh boar semen were place into an extender, Androhep, and stored at $4^{\circ}C$. In three of these samples, cryoprotectants were added. The sperm's motilities and kinematics were evaluated by using microscope (${\times}400$) and the viability status was evaluated by using with eosin staining method. The 5 sample groups are; Goup A:Androhep (extender), stored at $17^{\circ}C$. Group B:Androhep (extender), stored at $4^{\circ}C$. Group C:Androhep (extender), + 3% glycerol (cryoprotectant), stored at $4^{\circ}C$. Group D:Androhep (extender), + 3% DMSO (cryoprotectant), stored at $4^{\circ}C$. Group E:Androhep (extender), + 3% ethylene glycol (cryoprotectant), stored at $4^{\circ}C$. In group A, the sperm's motility was reduced. On day one the sperm's motility was ($85.7{\pm}2.3$) and day 5 the motility was ($43.9{\pm}3.3$). In group B, C and D the sperm's motility were reduced to 0 on day 5. In group E the sperm's percentage of motility decreased. On day one the sperm's motility was ($42.0{\pm}0.5$) and day 5 the motility was ($2.3{\pm}0.3$). When comparing cryoprotectant in samples of boar sperm there is a slight improvement in the results when the use of Androhep Lite (extender), + 3% ethylene glycol (cryoprotectant), stored at $4^{\circ}C$ are used. Based on these results, ethylene glycol can protect sperm from heat shock at $4^{\circ}C$, but not satisfactory level. However, it showed the possibilities of liquid semen preservation at $4^{\circ}C$ by using cryoprotectant.

Improvement of Boar Semen Quality by Sperm Selection Using Magnetic Nano-particles (마그네틱 나노비드를 이용한 돼지 정자 품질의 향상)

  • Chung, KI-Hwa;Son, Jung-Ho
    • Journal of Life Science
    • /
    • v.26 no.8
    • /
    • pp.943-947
    • /
    • 2016
  • The objective of this study was to see if fairly simple magnetic nano-particle treatment enhances boar semen qualities. Boar semen samples were prepared from the swine AI center and samples were divided by 4 different motility groups (1, >90%; 2. 80~90%; 3. 70~80%; 4. <70%) using computer assisted sperm analysis (CASA) evaluation. Boar semen was extended using BTS extender and same number of magnetic nano-particles as total number of spermatozoa in each sample was treated for 20 min and collected for 5 min at room temperature. Sperm qualities such as motility and viability were evaluated by the CASA before and after treatment. Sperm abnormality and degree of agglutination were also evaluated under the microscopic examination before and after treatment. There were significant changes (p<0.05) on sperm motility from all 4 different groups in the average of 7.11% after treatment. The enhancement of sperm motility changes was more clear in the groups of lower sperm motile groups (<70% and 70~80%; 19.12±1.08% and 5.67±0.71%, p<0.05). The sperm motility character in terms of curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP) and linearity (LIN, %) showed also similar pattern but motility enhancement wear more clear in below 70% motile group. Average sperm viability was increased to 4% by magnetic nano-particles (p<0.05). The percentage of sperm abnormality was also reduced significantly (p<0.05) to the range of 3.7~4.5% before after treatment. The degree of sperm agglutination was also reduced in lower motility groups by the magnetic nano-particle purification.

Effect of Cryodiluents, Cryoprotectants, Pre-freezing Method and Total Time Required for Freezing on Post-thaw Viability of Boar Spermatozoa (돼지정자의 동결융해 후 활력 및 생존성에 대한 보존액, 동해보호제, 예비동결 및 동결처리시간의 영향)

  • 이장희;김인철
    • Korean Journal of Animal Reproduction
    • /
    • v.23 no.2
    • /
    • pp.165-174
    • /
    • 1999
  • Boar semen can be frozen successfully. However, there is a large variability in the extent of damage boar semen samples experiences during cryopreservation. This experiment was undertaken to find out factors that affect a post-thaw viability of boar spermatozoa. For this purpose, cryodiluents(BF5, LEY, Soejima and M-Soejima), cryoprotectants(glycerol. ethylene glycol, and propylene glycol), pre-freezing method(dryice-pellet, dryice-straw and L$N_2$vapour-st-raw) and total time required for freezing(2. 5, and 7 h) were compared as a factors. To investigate quality of semen during freezing process, motility(%), normal apical ridges(%, NAR), and proportion of living sperm(%) by flow cytometic analysis were assessed after collection, cooled, pre-frozen and post-thawing. Post-thaw motility of semen diluted with M-Soejima was 52.0%, respectively. When heparin, caffeine or heparin+caffeine was added to 2nd cryodiluent of M-Soejima during freezing process, the highest motility after thawing was shown at the addition of caffeine (2mM), with 61.7$\pm$2.9% of motility. M-Soejima with heparin or caffeine was significantly higher than that of controI(p<0.05). The result using glycerol(Gly), ethylene glycol(EG), propylene glycol(PG), and their mixture (Gly+EG and Gly+PG) as cryoprotectants, the highest motility was shown at the mixture treatment with Gly plus PG. However, the highest proportion of live spermatozoa was shown at Gly+EG, there was no significantly difference among treatments(p>0.05). When semen was pre-frozen with three manners(dryice-pellet, dryice-straw, and L$N_2$ vapor-straw), motility(%) of post-thaw spermatozoa was the highest in the L$N_2$ vapor-straw pre-freezing method of M-Soejima cryodiluent with 57.5% of motility, For a simple, economical and timesaving approach to freezing boar semen, total time required for freezing were 2, 5, and 7 hours, post-thaw motility were 43.8, 45.0 and 38.8%, NAR were 19.5, 22.7 and 28.5%, and viability were 20.8, 19.9 and 22.1%, respectively. This data suggests that boar semen diluted with M-Soejima cryodiluent contained caffeine, using mixture of glycerol and propylene glycol or ethylene glycol as cryoprotectants, frozen with 2 hours, can be taken better motility, NAR, and proportion of live spermatozoa.

  • PDF