• 제목, 요약, 키워드: Boar

검색결과 303건 처리시간 0.037초

GIS와 로지스틱 회귀분석을 이용한 멧돼지 서식지 모형 개발 (Wild Boar (Sus scrofa corranus Heude ) Habitat Modeling Using GIS and Logistic Regression)

  • 서창완;박종화
    • Spatial Information Research
    • /
    • v.8 no.1
    • /
    • pp.85-99
    • /
    • 2000
  • 보호동물사에 대한 서식지 분포정보는 개발압력이 심한 우리나라에서는 서식지 관리에 있어 매우 중요하다. 본 연구는 GIS와 로지스틱 회귀분석을 이용하여 멧돼지의 서식지 적합성모형을 개발하여 서식지 분포도를 작성하고 본 연구의 결과를 토대로 현재 우리나라의 멸종위기종과 야생동물보호종들의 서식지 관리를 위한 기초를 마련하고자한다. 서식지 모형을 구현하기 위한 연구내용 및 방법은 다음과 같다. 첫째, 멧돼지의 서식지 특성을 파악하기 위하여 GIS 자료를 구축하였다. 멧돼지의 위치파악을 위해 원격무선측정기법과 GPS를 이용하였다 둘째, 멧돼지의 서식지 이용과 유용성에 영향을 미치는 환경인자를 파악하기위해 원격무선측정기법과 GPS를 이용하였다. 둘째, 멧돼지의 서식지 이용과 유용성에 영향을 미치는 환경인자를 파악하기 위해 χ²검정(Chi-square test)을 실시하였다. 마지막으로, 최적 서식지의 분포를 예측하기위한 서식지 적합성모형 개발을 위해 로지스틱 회귀분석을 이용하였다. 본 연구의 결과는 다음과 같다. 첫째, 멧돼지의 서식지 이용특성 분석의 결과, 성별 그리고 활동유형별에서는 뚜렷한 차이를 볼 수 없으나, 계절별 그리고 서식지 유형별에서는 서식지 이용특성의 차이가 뚜렷한 것을 볼 수있었다. 둘째, 서식지 유용성 분석의 결과 멧돼지의 서식환경에 영향을 미치는 자연적인 환경요인으로 표고, 향, 임상 그리고 영급이 유의성이 있는 인자로 나타났다. 반면에, 경사, 능선/계곡 , 물 그리고 일사량의 경우 통계적 유의성이 적어 전반적으로 서식지를 결정하는 환경인자라고 볼 수 없었다. 마지막으로, 개발된 서식지 적합성모형의 결과 예측확률 0.5를 기준으로 전제서식지는 75.00%, 커버서식지는 84.26% 의 높은 분류정확도를 나타내었다. 모형검증의 결과 대상지 내부의 경우 전체서식지가 75.00%로 나타나 본 모형이 신뢰성이 있는 것으로 판단되었다.

  • PDF

돼지 액상정액 보존을 위한 Lactose-Egg Yolk와 $B\ddot{u}tschwiler$ 희석액의 비교 (Comparision of Preservation of Liquid Boar Semen between Lactose-Egg Yolk and $B\ddot{u}tschwiler$ Diluents)

  • 박창식;천용민;서직
    • 한국가축번식학회지
    • /
    • v.20 no.2
    • /
    • pp.101-109
    • /
    • 1996
  • 본 연구는 돼지 액상정액을 인공수정용 100ml 플라스틱 병에 보존하면서 Lactose-Egg yolk 희석액과 B tschwiler 희석액 간에 보존 온도별 차이를 조사하고, Lactose-Egg yolk 희석액에서의 글리세롤 농도의 효과를 조사하여 돼지 액상정액을 좀더 장기간 사용할 수 있는 방법을 찾고자 실시하였다. 돼지 액상정액을 5$^{\circ}C$ 냉장고에 보존하면서조사한 바에 의하면, 37$^{\circ}C$에서 0.5 및 2시간 배양 후의 정자 운동성은 전체 보존기간동안 Lactose-Egg yolk 희석액이 B tschwiler 희석액보다 유의하게 (P,0.05) 높게 나타났고, 정상첨체비율은 두 희석액간에 차이가 없었다. 돼지 액상정액을 15$^{\circ}C$에 보존하면서 조사한 바에 의하면, 3일부터 7일 보존시까지 정자 운동성과 정상첨체비율에 있어서 B tschwiler 희석액이 Lactose-Egg yolk 희석액보다 유의하데 높게 나타났다. Lactose-Egg yolk 희석액을 이용한 돼지 액상정액의 글리세롤 농도의 효과에 있어서는 최종 글리세롤 농도가 0, 1, 3 및 5%보다 2%일 때 가장 높은 정자 운동성과 정상첨체비유을 나타내었다. 분만율, 복당 생존자돈수 그리고 출생시 평균 생시체중은 Lactose-Egg yolk 희석액과 B tschwiler 희석액간에 차이가 없었다. 이상의 연구 결과를 종합해 볼 때 Lactose-Egg yolk 희석액은 5$^{\circ}C$에서 B tschwiler 희석액은 15$^{\circ}C$에서 6~7일 동안 돼지 액상정액을 보존할 수 있었다.

  • PDF

Hamster test를 이용한 가축정자의 수정능력 검정 II. 정액배지 및 X-선조사가 돼지정자의 Hamster test에 미치는 영향 (Assessment of the Fertilizing Capacity of Domestic Animal Spermatozoa by Hamster Test II. Effects of incubation medium and X-ray irradiation on hamster test for boar spermatozoa)

  • 김용준;지동범
    • 한국임상수의학회지
    • /
    • v.9 no.2
    • /
    • pp.373-390
    • /
    • 1992
  • To assay the fertilizing capacity of domestic animal spermatozoa by hamster test, semen were collected from 13 boars(Duroc. Landrace and Yorkshire) which had been proved to be fertile in the past. then, were preserved in BWW medium or in raw state at 18$^{\circ}C$ or at room temperature. The preserved semen were given each different treatment according to the experimental design and coincubated with zona-free hamster ova for 5 hours. The ova were stained by lacmoid and examined under phase contrast microscope to investigate the rates of ova bound with sperm(sperm binding). ova penetrated by sperm(penetration) and formation of a male pronucleus(pronucleus formation) and also numbers of both bound and penetrated sperm per ovum. Between BWW and TBM medium for boar sperm. no difference in the results of hamster test was obtained. The boar spermatozoa in BWW medium, BWW with caffeine, BWW with heparin, and BWW with both caffeine and heparin showed no difference in the results of hamster test. The boar spermatozoa in BWW medium containing both calcium and RSA showed considerably higher rates of sperm binding, penetration and pronucleus formation as well as higher numbers of both bound and penetrated sperm than those not containing calcium with or without BSA( p<0.01) and also the same results higher than that containing calcium without BSA( p< 0.05). The boar spermatozoa irradiated by X-ray(70 KVP, 20mA) for 3 seconds. then, maintained at 18$^{\circ}C$ for 18 hours showed considerably lower rate of sperm binding than all the other groups including the control and X-ray groups irradiated by smaller dose or maintained for shorter period(p<0.01), and also showed lower number of bound sperm than the other groups(p<0.01, p<0.05). All the control groups of both raw and diluted sperm in BWM medium showed higher rates of sperm binding, penetration and pronucleus formation as well as higher number of penetrated sperm than all the X-ray groups irradiated for 3 seconds(70KVP, 20mA) and maintained for either 3 or 18 hours (p<0.01, p<0.05). At the same time the control groups of diluted sperm showed considerably higher rates of sperm penetration and pronucleus formation than the control group of raw sperm( p<0.01). These results indicates that fertile boar sperm showed considerably lower rates In the results of hamster test, when incubated in the medium without calcium and irradiated by X-ray than when incubated in the medium with calcium and not irradiated by X-ray, respectively, to prove consequently that hamster test would be of great value in assaying the fertilizing capacity of boar spermatozoa.

  • PDF

Hamster test를 이용한 가축정자(家畜精子)의 수정능력(受精能力) 검정(檢定) 1. 돼지정자의 보존온도(保存溫度) 비교 및 돼지와 개정자의 hamster test결과 (Assessment of the fertilizing capacity of domestic animal spermatozoa by hamster test I. Comparison of storage temperatures for boar sperm and results of hamster test between boar and dog sperm)

  • 김용준
    • 대한수의학회지
    • /
    • v.32 no.3
    • /
    • pp.435-450
    • /
    • 1992
  • To evaluate the fertilizing capacity of domestic animal spermatozoa by hamster test, semen were collected from 15 boars(Duroc, Landrace, and Yorkshire) and 2 mixed dogs which had been proved to be fertile in the past then, the semen were preserved in BWW medium at $4^{\circ}C$ or $18^{\circ}C$ for about 20 hours and coincubated with zona-free hamster ova for 5 hours. The ova were stained by lacmoid and examined under phase contrast microscope to investigate the rates of sperm binding to the ova, penetration and formation of a male pronucleus, and the numbers of both bound and penetrated sperm per ovum. Both the semen preserved at $18^{\circ}C$ for about 20 hours and that treated by swim up procedure showed considerably higher rates of sperm binding and penetration as well as higher number of penetrated sperm than that preserved at $4^{\circ}C$ for about 20 hours, respectively(p<0.01). Motility of boar sperm at insemination was from 40 to 90% and no difference in hamster test was obtained according to different degree of sperm motility. Abnormality in morphology of boar sperm at insemination was from 6 to 45% and no difference in hamster test was obtained according to different degree of sperm abnormality. The sperm concentrations of $7{\times}10^7$ and $7{\times}10^6$ showed considerably higher rates of sperm binding and penetration as well as higher number of bound sperm than that of $7{\times}10^4$ (p<0.01) along with the same higher results than that of $7{\times}10^5$(0<0.05), respectively. Boar sperm showed considerably higher rates of sperm binding and penetration as well as higher numbers of both bound and penetrated sperm than dog sperm, when both semen were treated by BWW+heparin medium and swim up procedure, respectively. These results indicated that fertile boar sperm showed considerably lower rates in the results of hamster test, when preserved at $4^{\circ}C$ for about 20 hours and in lower concentration of sperm than when preserved at $18^{\circ}C$ for about 20 hours and in higher concentration of sperm, respectively, and at the same time considerably higher results than fertile dog sperm, consequently to prove that hamster test would be of great value in assaying the fertilizing capacity of boar sperm.

  • PDF

돼지 정액의 간편 동결 방법 확립과 동결 정액의 융해 후 생존성 평가 (Establishment of the Convenient Boar Semen Freezing Method and Assessment of Viability in Frozen/Thawed Boar Semen)

  • 김성곤;장현용;박동헌;박춘근;정희태;김정익;양부근
    • Reproductive and Developmental Biology
    • /
    • v.30 no.1
    • /
    • pp.59-64
    • /
    • 2006
  • 본 연구는 돼지정액의 보다 간편하고 손쉽게 동결시킬 수 있은 방법을 확립하기 위하여 수행하였다. 돼지정액은 3시간에 걸쳐 $5^{\circ}C$까지 냉각 후 Straw에 봉입하고 다양한 방법 및 step에 의해 스티로폼 용기 내에 들어있는 $LN_2$ 중에서 동결하였다. 정자의 생존성은 $LN_2$ 표면으로부터 10 cm 위에서 10분간 정치 후 침적할 경우 가장 높게 나타났다(54.0%). Straw를 $-102^{\circ}C$에서 10분간 정치시킨 처리구가 여타구보다 높은 생존성이 얻어졌다(74.0%, P<0.05). 응해 방법에 따른 동결정액의 생존성 실험에서는 $37^{\circ}C$의 융해구가 $52^{\circ}C$ 융해구보다 유의적으로 높은 결과를 나타냈다(P<0.05). 1단계 동결 방법과 3단계 동결방법으로 돼지 정액을 동결시킨 결과 정액의 일반적 특성 및 첨체의 이상 유무를 평가하는 CTC 검사에서는 커다란 차이가 인정되지 않았다. 동결정액을 이용한 체외수정 결과에서는 상실배기 이상 발육율에서 1-step이 3-step보다 높은 발육율을 나타내었다(27.5 vs 14.7%, P<0.05). 본 실험의 결과 돼지정액 동결 시 $-102^{\circ}C$에서 10분간 정치시키는 1단계 동결방법이 간편하면서 유리한 동결 방법으로 활용될 수 있음을 보여준다.

돼지의 자연성비와 정자의 전기분이에 의한 수태성적에 관한 연구 (A Study on the Natural Sex Ratio and Fertility of Galvanized Boar Semen)

  • 이용빈;오봉국;권종국;서국성;정영철;오성종
    • 한국가축번식학회지
    • /
    • v.3 no.1
    • /
    • pp.56-60
    • /
    • 1979
  • This study was carried out to find the difference between the naturaly born sex rtio among 1,242 head of pigs(120 litters) at Swine Farm, Cheil Sugar Co. and B-body a, pp.arance from their semen, and to find the conception rates which were inseminated to 40 sows with sperm from the anode and cathode after electrophoresis of boar semen. In order to the electrophoretic separation, the semen was placed into the platimum loop electrodes(105 cc) at room temperature for 30 minutes with D.C. 3V. and 350${\mu}$A. constant. The sperm fluorescent staining method was performed in accordance with Bhattacharya's(1970) method. The spermatozoa were observed through a Olympus Vanox microscope(made in Japan) using exciter filter with I heat barrier HPO 120. The results obtained were summarized as follows: 1. The natural sex ratio of 1,242 piglets(120 litters) which were born at Swine Farm, Cheil Sugar Co. was 50%, and B-body a, pp.arance of its boar semen were 49.24%. 2. With electrophoretic separation, the anode and cathode attracted 65.5${\pm}$5.03% and 29.89${\pm}$4.29% of B-body bearing sperm, respectively. 3. After electrophoresis of boar sperm, they were inseminated to 40 sows with sperm from anode and cathode. The conception rate was 92.5%.

  • PDF

$\alpha$-Tocopherol과 Selenium이 웅돈의 정액성상에 미치는 효과 (Effects of $\alpha$ -Tocopherol and Selenium on the Boar Semen Characteristics)

  • 김광현;강만종;문승주
    • 한국가축번식학회지
    • /
    • v.25 no.2
    • /
    • pp.113-118
    • /
    • 2001
  • 본 연구는 $\alpha$-tocopherol과 selenium 첨가급여가 웅돈의 정액성상에 미치는 효과를 검토하였다. $\alpha$-tocopherol과 selenium 첨가급여가 웅돈의 정액량, 정자농도, 정자의 운동성, 기형정자율 그리고 수소이온농도(pH)에 미치는 영향을 조사한 결과 정액량과 수소이온농도는 처리간에 유의차가 없었으며(P<0.05) 정자의 농도, 총정자수, 정자의 운동성은 첨가구가 대조구에 비하여 유의적으로 높았으며 기형정자율은 유의적으로 낮았다(P<0.05). 한편, 정자 저장기간별 정자의 운동성은 대조구에 비하여 처리구에서 유의적으로 높은 정자운동성을 보였다(P<0.05). 이러한 결과는 $\alpha$-tocopherol 과 selenium 의 사료내 첨가 급여는 웅돈정액성상에 영향을 미치는 것으로 사료된다.

  • PDF

Using habitat suitability model for the wild boar (Sus scrofa Linnaeus) to select wildlife passage sites in extensively disturbed temperate forests

  • Rho, Paikho
    • Journal of Ecology and Environment
    • /
    • v.38 no.2
    • /
    • pp.163-173
    • /
    • 2015
  • The occurrence of wild boars (Sus scrofa Linnaeus) and reports of wildlife-vehicle conflicts (i.e., road-kill) involving them have increased in natural forest regions of Korea. In the past few decades, many wildlife passages have been constructed to reduce vehicle collisions involving wildlife species. However, few studies have assessed the habitat suitability of target wildlife species when locating the construction sites of wildlife passages. Target species rarely use wildlife passages if built in an inappropriate location. Therefore, a quantitative habitat model is required to find suitable sites for wildlife passages that can connect the fragmented forest patches of wildlife habitats in Korea. In this study, the wild boar was selected as the target species, and six environmental variables (percentage of Quercus forest, slope aspect, distance to roads, water accessibility, forest stand age and density) were measured. The habitat model for wild boars was developed with a Delphi survey, and habitat suitability maps were delineated for the provinces of Gangwon-do and Jeollanam-do. In this study, 298 and 64 boars were observed in Gangwon-do and Jeollanam-do, respectively. Observations of wild boars derived from the second nationwide natural environmental survey were used to evaluate the habitat model. Habitat suitability maps that superimposed existing road networks suggested that wild boar habitats were severely fragmented in both provinces, particularly in Gangwon-do. To connect the fragmented habitats and prevent wildlife-vehicle collisions, this study proposes 11 and 5 wildlife passage sites in Gangwon-do and Jeollanam-do, respectively.

Seroprevalence of Trichinella sp. in Wild Boars (Sus scrofa) from Yanggu-gun, Gangwon-do, Korea

  • Lee, Hye-Jung;Chung, Ok-Sik;Kim, Jae-Lip;Lee, Seung-Ha;Yoo, Young-Bok;Seo, Min
    • The Korean Journal of Parasitology
    • /
    • v.53 no.2
    • /
    • pp.233-236
    • /
    • 2015
  • A total 7 outbreaks of trichinellosis have occurred in Korea, mostly as a result of consumption of raw wild boar (Sus scrofa) meat. Since only 1 serological survey on wild boars had yet been performed in Korea, the present study aimed to estimate the prevalence of trichinellosis in wild boars and some species of rodents by artificial digestion and serological examinations in Yanggu-gun, Gangwon-do, the endemic area of trichinellosis. Both the wild boar and rodent muscle samples revealed no Trichinella larvae by direct examination and artificial digestion method. However, serological examinations revealed that 4 wild boar sera samples out of 118 (3.4%) were positive to Trichinella antigen. Although the recovery of Trichinella larvae ended in a failure, it is proved for the first time that the sylvatic cycle of Trichinella has been maintained in wild boars of Gangwon-do, Korea.

Effect of Alpha-Linolenic Acid with Bovine Serum Albumin or Methyl-Beta-Cyclodextrin on Membrane Integrity and Oxidative Stress of Frozen-Thawed Boar Sperm

  • Lee, Won-Hee;Kim, Wook-Hwan;Cheong, Hee-Tae;Yang, Boo-Keun;Park, Choon-Keun
    • 한국발생생물학회지:발생과생식
    • /
    • v.23 no.1
    • /
    • pp.11-19
    • /
    • 2019
  • The study was conducted to investigate the effects of alpha-linolenic acid (ALA) combined with bovine serum albumin (BSA) or methyl-beta-cyclodextrin (MBCD) on plasma and acrosomal membrane damages, mitochondrial activity, morphological abnormality, motility, and oxidative stress in frozen-thawed boar sperm. In previous our study, 3 ng/mL ALA had been shown protective effect during freezing process of boar sperm. Therefore, we used 3 ng/mL ALA in present study and ALA was combined with same molar ratio of BSA or MBCD (ALA+BSA and ALA+MBCD, respectively). To confirm the effect of two carrier proteins, same volume of BSA and MBCD without ALA were added during cryopreservation. Membrane damage, mitochondrial activity, reactive oxygen species (ROS) and lipid peroxidation (LPO) levels were measured using flow cytometry, and movement of sperm tail as motility parameter and morphological abnormality were observed under light microscope. In results, all of sperm parameters were enhanced by ALA combined with BSA or MBCD compared to control groups (p<0.05). Mitochondrial activity, morphological abnormality, ROS and LPO levels in ALA+BSA or MBCD groups were no significant difference compared with ALA, BSA and MBCD treatment groups. On the other hand, plasma and acrosomal membrane intact, and sperm motility in ALA+MBCD group were higher than single treatment groups (p<0.05), whereas ALA+BSA did not differ. Our findings indicate that carrier proteins such as BSA and MBCD could improve the effect of ALA during cryopreservation of boar sperm, and treatment of ALA with carrier proteins enhance membrane integrity, mitochondrial activity through reduction of ROS-induced LPO.