• Title/Summary/Keyword: Biosurfactant

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Surface-activity and Environmental Characteristics of Biosurfactant Produced by Pseudomonas aeruginosa JRT-4 (Pseudomonas aeruginosa JRT-4에 의해 생산된 Biosurfactant의 계면활성 및 환경적 특성)

  • 황경아;이정래;김상종;김윤석;안호정
    • Microbiology and Biotechnology Letters
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    • v.27 no.2
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    • pp.159-165
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    • 1999
  • Pseudomonas aeruginosa JRT-4 strain was used as a biosurfactant-producing microorganism in this study. It was one of the microorganisms isolated from the sewage sludge, the main and branch streams of Han river. The surface tension of the culture broth of P. aeruginosa JRT-4 decreased to 30mN/m. The crude biosurfactant was obtained from the culture broth by acid precipitation, solvent extraction, evaporation, and freeze drying. The CMC value of the crude biosurfactant was 0.006%(w/v). From analysis of the chemical structure of biosurfactant, it was determined as rhamnolipid 1 and 3 structures by FAB mass spectrometer. In the washing test for artificially contaminated textiles, the biosurfactant showed better bleachness than the two chemically synthesized surfactant, LAS and SLES. Finally, the biodegradation and ecotoxidolorical tests showed that the biosurfactant was readily biodegradable in the environment and a mild material for microorganisms and green algae.

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Production of Biosurfactant Using Bacillus spp (Bacillus spp.를 이용한 Biosurfactant 생산공정)

  • Hur, Sung-Ho;Yang, Ji-Seok;Hong, Jeong-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.3
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    • pp.389-393
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    • 2002
  • Among the bacterial strains isolated from chungkook-jang, Bacillus subtilis CH-1, Bacills circulans K-1 and Bacillus subtitis (natto) N-1, Bacillus subtitis CH-1 showed the highest productivity of biosufactant. A-medium was selected for the basal medium in the large scale production of biosurfactant, and modified to synthetic medium which containing 2% glucose, 0.3% soy peptone, and mineral salts. The surface tension was reduced to maximal value after 96 hr after fermentation, about the 43% of initial tension. Temperature and initial pH of medium was not critical factor for the biosurfactant production. The yield of crude biosurfactant was 6 g/L under the optimum condition.

Isolation of Biosurfactant-Producing P. aeruginosa Mi-7 and the Biosurfactant Production (Biosurfactant를 생산하는 P. aeruginosa. KK-7의 분리 및 Biosurfactant의 생산)

  • 강상모;김대원;김혜자
    • Microbiology and Biotechnology Letters
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    • v.22 no.1
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    • pp.92-98
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    • 1994
  • The bacteria which secrete surface-active agent and decrease the surface tension of culture broth were isolated from soil samples. Among them, biosurfactant producing strain KK-7 was selected and emulsification was also detected. The KK-7 produced biosurfactant not only lipid but also glucose by using carbon source. Taxonomical characterization tests have demostrated the strain KK-7 to be Pseudomonas aeruginosa. The media composition of the P. aeruginosa KK-7 for the biosurfactant production was 1% glucose, 0.5% tryptone, 0.2% yeast extract, 0.15% potas sium phosphate mono-dibasic, 0.05% MgSO$_{4}$, initial pH 8.5, at 30$\circ $C for 2 days. In this condition, the concentration of biosurfactant was reached CMC 5 in the culture broth. Surface active material was produced maximum at stationary8 phase, but emulsification power was higher at log phase than stationary phase. It was considered that P. aeruginosa KK-7 produced biosurfactant more than one type having defferent properties and each maximum production time was different. The minimun surface tension of biosurfactant in 50 mM Tris buffer (pH8.0) was 28 dyn/cm, and CMC was 1 g/L.

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Isolation and Characteristics of Biosurfactant Producing Bacterium, Bacillus sp. TBM 911-5 (Biosurfactant 생산균주 Bacillus sp. TBM 911-5의 분리 및 특성)

  • 김선희;정연주;이상철;유주순;주우홍;정수열;최시림;최용락
    • Journal of Life Science
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    • v.14 no.2
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    • pp.320-324
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    • 2004
  • The objective of this study was investigate the characteristic of biosurfactant produced from the isolated strain. The strain was isolated from soil samples and identified as Bacillus sp. TBM 911-5 by physiological characteristics and the partial nucleotide sequence analysis of 16S rDNA. We measured the surface tension every 6 hours for 80 hours. The surface tension of the culture filtrate of Bacillus sp. TBM 911-5 was decreased to 29 mN/m. Biosurfactant concentration was determined by diluting the culture filtrate until the critical micelle concentration (CMC). The biosurfactant emulsified hydrocarbons, vegetable oil and crude oil. Using soybean oil as substrate, the maximum emulsification activity and stability was obtained from the biosurfactant. The biosurfactant produced from Bacillus sp. TBM 911-5 had strong properties as an emulsifying agent and an emulsion-stabilizing agent.

Isolation and Characterization of Biosurfactant from Bacillus atrophaeus DYL,-130 (Bacillus atrophaeus DYL-130이 생산하는 biosurfactant의 분리 및 특성)

  • Kim Sun-Hee;Lee Sang-Cheol;Park In-Hye;Yoo Ju-Soon;Joo Woo-Hong;Hwang Cher-Won;Choi Young-Lark
    • Journal of Life Science
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    • v.15 no.5 s.72
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    • pp.679-684
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    • 2005
  • The objective of this study was investigate the characteristic of biosurfactant produced from the iso-lated strain. The strain was isolated from soli samples of Duck-Yu Mountain and it was identified as Bacillus atrophaeus DYL-130 by 16S rDNA and gyrA gene nucleotide sequence analysis. The surface ten-sion of culture filtrate of Bacillus atrophaeus DYL-130 decreased to 28 mN/m and its biosurfactant con-centration was determined by diluting the culture filtrate until the critical micelle concentration (CMC). The emulsifying activity and stability of crude biosurfactant was measured by using water-immiscible hydrocarbons and oils as substrate. The biosurfactant was purified by affinity chromatography and the surface activity of purified biosurfactant was measured by drop-collapsing method and it could be effectively emulsify toluene.

Cosmical Analysis and Interfacial Characterization of Biosurfactants formed by Rhodococcus. Sp. strain IGTS8 during the Biodesulfurization Process (미생물 탈황 공정 중 Rhodococcus sp. strain IGTS8에 의하여 생성되는 Biosurfactants의 성분 분석 및 계면특성)

  • 박홍우;박기돈;오성근
    • KSBB Journal
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    • v.17 no.3
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    • pp.302-306
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    • 2002
  • The chemical analysis and surface chemical properties of biosurfactant formed by Rhodococcus sp. strain IGTS8, which is widely used in biodesulfurization process, in hexadecane/water mixture have been studied. For the chemical analysis, TLC technique was employed. The surface tension, CMC, and emulsion stability of biosurfactant solution were also investigated. The major components of biosurfactant formed by Rhodococcus sp. strain IGTS8 were glucose mycolate and trehalose monomycolate. The CMC of aqueous biosurfactant solution was 0.1 ~0.15 g/100 mL of Water at pH 6.0-6.5 and pH 10~10.5. But the demulsification was faster at pH 10 than at pH 6.3.

Effect of pH and Temperature on the Production of Biosurfactant by Pseudomonas aeruginosa YPJ-80 and Its Separation (Pseudomonas aeruginosa YPJ-80에 의한 생물계면활성제 생산에 미치는 pH 및 온도의 영향과 생물계면활성제의 분리)

  • 박창호;손창규;김성훈;안도균
    • KSBB Journal
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    • v.13 no.5
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    • pp.511-517
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    • 1998
  • Temperature and pH conditions were studied for an effective biosurfactant production by Pseudomonas aeruginosa YPJ-80. Efficient methods of biosurfactant separation were also investigated. pH-uncontrolled experiments at 35$^{\circ}C$ and an initial pH of 8 resulted in the best cell growth (3.6 g/L) and biosurfactant production (0.073 g biosurfactant/g cell). Biosurfactant separation was most efficient using solvent extraction with chloroform/methanol (2:1 vol%) followed by acidification using 1N HCl.

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Cloning of Biosurfactant-Producing Gene from Bacillus subtilis KL-57 (Bacillus subtilis KL-57로부터 생산되는 생체계면활성제 합성 유전자 클로닝)

  • 강상모;이병옥;이철수
    • Microbiology and Biotechnology Letters
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    • v.22 no.6
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    • pp.593-598
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    • 1994
  • A bacterium KL-57 which exhibited biosurfactant activity was isolated. This bacterium was identified as Bacillus subtilis. The biosurfactant-producing gene of B. subtilis KL-57 was cloned into R subtilis MI113 by using plasmid pTB523. The plasmid DNA from the clone was found to carry a 18 kb PstI insert. The biosurfactant-producing gene was cleaved into 4 fragments by SmaI, 3 fragments by PvulI or EcoRl, 4 fragments by PvulI and EcoRI double digestion, 5 fragments by AccI, and 2 fragments by KpnI, HindIII or BamHI. By subcloning the 18 kb Pstl insert, a 2.3 kb EcoRl fragment conferred the biosurfactant producing activity on B. subtilis cells. The 2.3 kb had one HindIII cleave site. But Two fragments, which corresponds HindIII/EcoRl termini, exhibited no biosurfactant activity.

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Purification and Physical Proerties of Biosurfactant Produced from Rhodotorula muciloginosa (Rhodotorula muciloginosa G-1에서 생산되는 biosurfactant의 정제 및 물리적 성질)

  • 이철수;이병옥;강상모
    • Microbiology and Biotechnology Letters
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    • v.23 no.2
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    • pp.229-235
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    • 1995
  • The surface tension-decreasing biosurfactant was purified from Rhodotorula muciloginosa G-1. The purification procedure was the solvent extraction of culture broth. To ensure complete extraction, the sample was extracted twice with equal volume of ethylacetate. The crude solution was washed with n-hexane to remove unconsumed soybean oil. The crude sample of biosurfactant was applied to Silica gel column chromatography equilibrated with chloroform, and eluted with chloroform : methanol gradient. Serveral solvent system was used to developed the thin layer chromatography (TLC). The purified biosurfactant sample gave one spot (Rf 0.78). It was estimated that biosurfactant was glycolipid about having M.W.1,500 with standard of polyethyleneglycol by Sephadex LH-20 column chromatography.

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Environmental toxicity and biodegradation of Pseudomonas sp. EL-G527 producing biosurfactant

  • Mi Seon, Cha;Hong Ju, Son;Sang Jun, Lee
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2002.05b
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    • pp.452-454
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    • 2002
  • A biosurfactant-producing microorganism, .Pseudomouos sp. EL-G527 was isolated from activated sludge by enrichment culture when grown on mineral salt medium containing n-hexadecane as a carbon source. The biosurfactant from .Pseudomonar sp. EL-G527 exhibited lesser toxicity to bacterial population than synthetic surfactants and in the biodegradation test, biosurfactant was rapidly degraded and lost its activity as surface active material after 1 day incubation. In this study, the biosurfactant from Pseudomonas sp. EL-G527 was effective surface-active compound, more biodegradable and less toxic to microbial ecosystem than various synthetic surfactants.

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