• Title, Summary, Keyword: Bacillus thuringiensis subsp. morrisoni PG-14

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Characterization of Synechocystic PCC6803 transformed with cryIVD gene of Bacillus thuringiensis subsp. morrisoni PG-14 and its mosquitochidal effect on Anopheles sinensis (Bacillus thuringiensis subsp. morrisoni PG-14 cryIVD 유전자로 형질전환된 Synechocytis PCC6803의 특성과 학질모기에 대한 살충효과)

  • 이대원;박현우;김호산;진병래;유효석;김근영;강석권
    • Korean journal of applied entomology
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    • v.35 no.1
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    • pp.66-73
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    • 1996
  • For the effective control of mosquito larvae, Anopheles sinensis, the expression vector pCYASK5-1 containing cryIVD gene of Bacillus thuringiensis subsp. morrisoni PG-14 was constructed and transformed into the cyanobacterium Synechocystis PCC6803. The transformants were selected on BG-11 medium containing kanamycin. The expression of cryIVD gene in transformant was confirmed by SDS-PAGE and Western blot analysis. The mortality of A. sinensis larvae was scored for 3 days. Furthermore, growth and distribution rate of transformant were examined. The results showed that Synechocystis PCC6803 transformed with cryIVD gene of B. thuringiensis subsp. morrisoni PG-14 was highly toxic to A. sinensis larvae, demonstrating that it will be a potential agent for mosquito control.

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Comparison of Characteristics between Insecticidal and Noninsecticidal Basillus thuringiensis Strains belonging to Serotype H8a8b

  • Roh, Jong-Yul;Je, Yeon-Ho;Park, Hyun-Woo;Chang, Jin-Hee;Jin, Byung-Rae;Lee, Dae-Weon;Ziwen Yang;Kang, Seok-Kwon
    • Journal of Sericultural and Entomological Science
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    • v.40 no.2
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    • pp.126-130
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    • 1998
  • A noninsecticidal strain, Bacillus thuringiensis NTB-88, isolated from Korean soil, had a typical bipyramidal parasporal inclusion and its serotype is identical to B. thuringiensis subspmorrisoni (H8a8b). To elucidate differences between insecticidal and noninsecticidal strains, we compared strain NTB-88 to other toxic B. thuringiensis subsp. morrisoni strains (HD-12 and PG-14). Restriction endonucleases digested plasmid DNA patterns showed that strain NTB-88 was different from lepidopteran-toxic strain, HD-12, but it was similar to dipteran-toxic strain, PG-14. The gene type of strain NTB-88 was different from those of other insecticidal strains, Furthermore, the NH2-terminal amino acid sequence of crystal protein of strain NTB-88 had no relation to those of the previously known $\delta$-endotoxins in other toxic strains as well as HD-12 and PG-14 strains. Therefore, the noninsecticidal crystal protein in strain NTB-88 is novel and its property is different from insecticidal ones.

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Characterization of Two Type Crystal Proteins Produced by Transformed Bacillus thuringiensis NT0423 (형질전환된 Bacillus thuringiensis NT0423 균주에 의해 생산된 두가지 형태의 내독소 단백질의 특성)

  • 박현우;김호산;유용만;김상현;서숙재;강석권
    • Microbiology and Biotechnology Letters
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    • v.21 no.5
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    • pp.428-434
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    • 1993
  • Cloning and expression of two different crystal protein genes from transformed Bacillus thuringiensis were investigated. B. thuringiensis NT0423 is toxic to both Lepidopterous and Dipte-rous larvae. The pCG5 vector carrying crystal protein genes (mosquitocidal and hemolytic activity) of B. thurigiensis subsp. morrisoni PG-14 was transformed into B. thurigiensis NT0423. Transformant has expressed two type crystals of bipyramid from NT0423 and ovoid from pCG5 in one cell.

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Synergistic Effect in Mosquitocidal Activity of Crystal Proteins from Bacillus thuringiensis NT0423 Transformed with cryIVD Gene (cryIVD 유전자로 형질전환된 Bacillus thuringiensis NT0423 균주 내독소 단백질의 모기 유충에 대한 독성의 상승효과)

  • 박현우;유용만;오현우;진병래;김인기;강석권
    • Korean journal of applied entomology
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    • v.35 no.1
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    • pp.85-90
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    • 1996
  • Bacillus thuringiensis NT0423 produces quite a typical bipyramidal crystals of a common major band of ca. 130 kDa, and has dual specificity against Lepidoptera and Diptera. To enforce the Diptera-toxicity of B. thuringiensis NT0423, cryND gene was transformed 30 B. thuringiensis NT0423. The transfonnant B. thuringiensis PT1227 was obtained from introduction of pCGl0 into B. thuringiensis NT0423 by electroporation. The result showed that cryND and resident crystal protein genes in transformant were stably expressed with its own shape. Furthermore, the toxicity of B. thuringiensis PT1227 against Diptera was highly enforced, suggesting that the enforced toxicity of B. thuringiensis PT1227 was due to synergistic effect of both introduced and resident crystal proteins in transformant.

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Construction of shuttle vectors expressing the cry11Aa gene and their mosquitocidal activity (모기살충성 cry11Aa 유전자를 발현하는 벡터의 구축과 모기살충효과)

  • Lee, Dae-Weon;Kim, Ho-San;Je, Yeon-Ho;Kim, Joo-Up;Yu, Hyo-Sok;Kang, Seok-Kwon
    • The Korean Journal of Pesticide Science
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    • v.2 no.1
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    • pp.91-96
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    • 1998
  • For the expression of the cry11Aa gene highly toxic to dipteran insects, we constructed two cyanobacteria-Escherichia coli(E. coli) shuttle vectors, pCYASK5-l and pCYASK5-2. These vectors were transformed into E. coli and selected with kanamycin. The expression of the cry11Aa gene in E. coli was characterized by SDS-polyacrylamide gel electrophoresis and Western blot analysis. Two E. coli transformants harboring pCYASK5-1 and pCYASK5-2 expressed the cry11Aa gene in size of 72 kDa and 64 kDa, respectively and showed over 89% mortality against Culex pipiens larvae.

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