• Title, Summary, Keyword: BPH

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Screening of Resistance Genes Linked to Brown Planthopper Using STS Marker in Aromatic Rice Germplasm

  • Kim, Jeong-Soon;Ahn, Sang-Nag;Hong, Sung-Jun;Park, Jong-Ho;Lee, Min-Ho;Han, Eun-Jung;Damodaran, P.N.;Kim, Yong-Ki;Jee, Hyeong-Jin;Shim, Chang-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.2
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    • pp.167-176
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    • 2011
  • Brown planthopper (BPH) is a serious insect pest of rice crop throughout rice growing countries, and yield loss due to its infection can be up to 60%. This study aimed to evaluate efficiency of molecular markers for screening BPH resistance accessions among 86 aromatic rice germplasm Eighty-six accessions of aromatic rice germplasm included two accessions of Tongil type (bred in Korea), 28 accessions of japonica type and 56 accessions of indica type. We applied eight STS markers (pBPH9, pBPH19, pBPH20, pBPH21, AJ09-b, RG457L, RG457B, and 7312.T4A) which were linked to four of BPH resistance genes, Bph1, Bph13(t), Bph10, and Bph18(t) respectively. One japonica type accession, 415XIr352, and six indica type accessions possessed one or four positive bands when tested with four STS markers linked to Bph1 gene. One indica type aromatic rice, Basmati9-93, showed the target bands linked to the Bph10 gene. The other accessions did not show same fragments as the respective resistant lines. Bph13(t) is the most widely introduced resistance gene and only one accession showed positive bands implying that this accession might harbor Bph10 and Bph18(t) genes. Three aromatic accessions, Domsiah, Khao Dawk Mali 105 and 415XIr352 showed gene pyramiding of Bph1 and Bph13(t). Two indica aromatic rice, Ds 20 and Basmati 9-93, possessed at least two BPH resistance genes, Bph1, Bph18(t) and Bph13(t), Bph18(t), respectively. These results indicates that aromatic rice germplasm have narrow diversities of BPR resistance genes.

Benign Prostatic Hyperplasia in Community-Dwelling Elderly in Korea

  • Lee Eun-Hyun;Chun Ki-Hong;Lee Yunhwan
    • Journal of Korean Academy of Nursing
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    • v.35 no.8
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    • pp.1508-1513
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    • 2005
  • Background. This study was done to identify the prevalence of benign prostatic hyperplasia(BPH) and BPH-related symptoms among community-dwelling elderly men in Korea. In addition, quality of life and health care-seeking behavior were explored. Methods. A total of 417 elderly men were surveyed using the IPSS (International Prostatic Symptom Score) and a structured questionnaire on health care-seeking behaviors. Results. The prevalence of BPH was $19.7\%$. Of those with BPH, $80.3\%$ reported mild symptoms, $13.2\%$ moderate symptoms, and $6.5\%$ severe symptoms. The severity of BPH-related symptoms was significantly correlated with quality of life. Among those with BPH, $42.7\%$ had never consulted with anyone about their symptoms. Conclusions. BPH has emerged as a serious public health problem in elderly men. Elderly people who experience worse symptoms of BPH have a lower quality of life. Many elderly with BPH do not seek health care, mainly due to misconceptions about BPH. The provision of educational programs for BPH may significantly improve the quality of life of elderly men.

Cloning and Phylogenetic Analysis of Two Different bphC Genes and bphD Gene From PCB-Degrading Bacterium, Pseudomonas sp. Strain SY5

  • Na, Kyung-Su;Kim, Seong-Jun;Kubo, Motoki;Chung, Seon-Yong
    • Journal of Microbiology and Biotechnology
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    • v.11 no.4
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    • pp.668-676
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    • 2001
  • Pseudomonas sp. strain SY5 is a PCB-degrading bacterium [24] that includes two different enzymes (BphC1 and BphC2) encoding 2,3-dihdroxybiphenyl 1,2-dioxygenase and BphD encoding 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase. The bphC1 and bphC2 genes were found to consist of 897 based encoding 299 amino acids and 882 bases encoding 294 amino acids, respectively, whereas the bphD gene consisted of 861 bases encoding 287 amino acids. According to a homology search, a 50% and 39% similarity between the bphC1 and bphC2 genes at the nucleotide and amino acid level was shown, respectively. The bphC1 gene showed a 38% and 45% similarity at the amino acid level to Alcaligenes eutrophus A5 and Rhodococcus rhodochrous, respectively, whereas, bphC2 showed a 95% and 43% similarity, respectively. A comparison of the deduced amino acid sequence of the bphD product of Pseudomonas sp. SY5 with that of A. eutrophus A5, Pseudomons sp. KKS102, and LB400 showed a sequence identity of 92, 92, and 79%, respectively. Strain SY5 was originally isolated from municipal sewage containing recalcitrant organic compounds an found to have a high degradability of various aromatic compounds [23]. The current study found that strain SY5 had two extradiol-type dioxygenases, which did not hybridize with each other as they had a low similarity, yet a similar structure of evolutionarily conserved amino acids residues for catalytic activity between BphC1 and BphC2 was observed.

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Biological Response of Resistant Genes to Korean Brown Planthopper, Nilaparvata lugens Stål (벼멸구 저항성 유전자에 대한 국내 벼멸구의 생물적 반응 연구)

  • Choi, Nak Jung;Kim, Gwang-Ho;Baik, Chai-Hun;Lee, Bong-Choon
    • Journal of Life Science
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    • v.29 no.2
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    • pp.202-208
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    • 2019
  • Brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacidae), is one of the most important migratory pests damaging rice in Korea. It invades annually from tropical and subtropical areas via continental air streams. It is necessary to determine the resistance levels of rice varieties in order to control efficiency. The honeydew excretion, development, and reproduction of the migratory BPH were studied by region in a laboratory at $25{\pm}2^{\circ}C$ and $65{\pm}5%\;RH$ and a 16L: 8D photoperiodism conducted on three BPH resistant genes: Bph1, Bph2, and Bph18. The information obtained was reported using the jackknife method, and we created life table statistics accordingly. The feeding amount of Bph1 resistant gene was lower than that of resistant genes. The developmental periods of immature stages ranged from $13.7{\pm}0.10d$ on Bph2 (Namhae, 2015) to $18.5{\pm}1.06d$ on Bph2 (Sacheon, 2016). Reproductive period and female longevity were longest on the non-resistant genes, Bph2 and Bph18 (except 1980s), and the highest fecundity of N. lugens was observed on the two BPH resistant genes. Highest net reproductive rates ($R_0$) were calculated on Bph2 by region. Intrinsic rates of population increase ($r_m$) showed a difference in resistant genes by region. These population parameters showed that migratory regions and biological characteristics of N. lugens vary annually.

Studies on the Anti-bacterial, Anti-inflammatory and Anti-oxidant Effect of BPH (비피(鼻皮) 증류액의 항균, 소염, 항산화 작용에 대한 연구)

  • Kim, Ho-Seon;Lee, Young-Jong
    • The Korea Journal of Herbology
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    • v.29 no.5
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    • pp.45-53
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    • 2014
  • Objectives : The purpose of the study is to test the antibacterial, anti-inflammatory and antioxidant effects of BPH, which is composed of Pini Densiflorae Nodi Lignum and Querci Acutissimae Fructus, Angelicae Gigantis Radix, Cnidii Rhizoma, Angelicae Dahuricae Radix, Angelicae Tenuissimae Radix. Method : Antibacterial and anti-inflammatory effects of BPH on Propionibacterium acnes, one of anaerobic bacteria species were evaluated by measuring the levels of 2,2-diphenyl-1-picrylhydrazyl (DPPH) elimination and lipid peroxidation. Result : When BPH was applied to CCD-986sk (Human normal fibroblast) to confirm the level of cytokine(tumor necrosis factor-alpha, interleukin-8), its level increased in proportion to that of BPH's concentration, which indicated dose-dependent relationship. Using the Disk diffusion to measure the bacterial growth inhibition zone varying BPH concentration, it was found that the antibacterial effect of BPH was less than that of erythromycin, the control group, but was higher than that of saline, and it increased with higher concentrations. In a liquid culture medium containing BPH, the growth rate of Propionibacterium acnes was decreased by more than 10% at 25% BPH. After adding P. acnes to THP-1 monocyte, and treated it with BPH, and measuring the concentration of TNF-a and IL-8, it was observed that the amount of TNF-alpha and IL-8 significantly decreased depending on the level of BPH concentration. The ability to eliminate DPPH increased with higher BPH concentration. The inhibition of lipid peroxidation was increased by BHT treatment in a dose-dependent manner. Conclusion : Using Propionibacterium acnes, an anaerobic bacteria, we confirmed that BPH has antibacterial, anti-inflammatory and antioxidant effects.

Changes in the Fitness of Brown Planthopper, Nilapawata lugens Stal (Homoptera: Delphacidae) to Several Resistant Rice Varieties after Multi-generational Selection (누대도태에 의한 벼멸구의 품종적응성의 변화)

  • 송유한;황인철;김진호
    • Korean journal of applied entomology
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    • v.41 no.2
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    • pp.113-121
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    • 2002
  • This study investigated the changes in the fitness of brown planthopper, NilapaHata lugens, to several rice varieties with different resistance background, after multi-generational selection on a resistant rice variety. A susceptible strain of brown planthopper (Dongjin-5) had been reared on the Chungchungbyeo with Bph1 resistance gene for three generations (Chungchung-G3) and six generations (Chungchung-G6), then the fitness change was evaluated by measuring their longevity, fecundity, preferences, and survivorships on the varieties with various background of resistance. After being selected three to six generations on Chungchungbyeo, feeding preference, adult longevity, and fecundity increased, where as nymphal period reduced when they were reared on various varieties with Bph1 gene. The egg Periods were not much different among the varieties fed on, except for the Chungchung-G6 on the rice varieties of Milyang63 (bph2 gene) and Gayabyeo (Bph1+bph2 gene). These results suggest that the susecptible Donajin-5 (Biotype-1) can be easily converted to a resistant biotype-2 capable of overcoming the resistant varieties with Bph 1 gene.

Relations between Brown Planthopper (BPH) Resistance and Sucking Inhibitors of BPH in Rice

  • Kim, Sung-Eun;Kim, Young-Doo;Nam, Jeong-Kwon;Kim, Bo-Kyeoung;Ko, Jae-Kwon;Kim, Jung-Gon;Lee, Jin-Ho;Chun, Jae-Chul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.6
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    • pp.513-518
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    • 2006
  • This study was conducted to investigate the relations between brown planthopper (BPH, Nilapavata lugens) resistance and specific organic acids (oxalic acid, silicic acid, and trans-aconitic acid) known as BPH sucking inhibitors on different rice varieties and/or lines. There were no specific relations between BPH resistance and the contents of oxalic and silicic acids in the rice plant tissues. However, the stronger the BPH resistance was occurred, the higher the content of trans-aconitic acid was contained in the rice plants. The relations between the injury rate of rice plant by BPH and the content of trans-aconitic acid in the rice plants were negatively correlated, which were -0.84 and -0.82 at 30 and 60 days after seeding, respectively. Therefore, the content of trans-aconitic acid in rice plant tissues might be utilized as an index for improving BPH resistance of rice varieties.

Molecular Mapping of Resistant Genes to Brown Planthopper, Bphl and bph2, in Rice

  • Cha, Young-Soon;Cho, Yong-Gu;Shin, Kyeong-Og;Yeo, Un-Sang;Choi, Jae-Eul;Eun, Moo-Young
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.4
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    • pp.345-349
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    • 1999
  • This study was carried out to map Bphl and bph2 gene in Mudgo and Sangju13 (Oryza sativa L.) respectively conferring resistance to brown plan-thopper (BPH) and to establish the marker-assisted selection (MAS) system. Bulked seedling (grown for 20 days) test was conducted with the 73 F4 lines derived from a cross between Nagdongbyeo and Mudgo for Bphl and with 53 BC3F5 lines derived from the Milyang95/Sangju13 cross for bph2. Bph1 was mapped between RG413 and RG901 on chromo-some 12 at a distance of 7.5 cM from RG413 and 8.4 cM from RG90l. A recessive gene bph2 was located near RZ76 on chromosome 12 at a distance of 14.4 cM. Bphl and bph2 were linked to each other with a distance of about 30 cM. An RFLP marker, RG413 linked to Bphl, was converted to an STS marker to facilitate the marker-assisted selection. BPH resistant genotypes could be selected with 92% accuracy in a population derived from a line of NM47-B-B.

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Effect of Brown Planthopper Resistance Gene, Bph18 to Yield Components in Rice (벼멸구 저항성 Bph18 유전자가 벼의 수량구성요소에 미치는 영향)

  • Shin, Mun-Sik;Kim, Woo-Jae;Shin, Woon-Chul;Park, Hyun-Su;Seo, Chun-Sun;Choi, In-Bae;Ha, Ki-Yong;Kang, Hyun-Jung;Ko, Jae-Kwon
    • Korean Journal of Breeding Science
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    • v.43 no.1
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    • pp.56-61
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    • 2011
  • Brown planthopper(BPH) is one of the most destructive insect pests of rice. The use of genetically resistant cultivars has proven to be a more economical, efficient and environment friendly means to combat this pest. This study was carried out to investigate the relationship between BPH resistant gene, Bph18 and yield components of rice using DH(doubled haploid) lines derived from 'Saenuri'/SR30071-3-7-23-6-1-1-1. SR30071-3-7-23-6-1-1-1 line has Bph18 gene derived from wild species, Oryza australiensis. BPH resistant gene, Bph18 shortened heading days, enlarged culm length and panicle length and reduced ratio of ripened grains of rice. The results indicate that backcrossing breeding is necessary to develop elite cultivars carrying Bph18 gene.

Versatile Catabolic Properties of Tn4371-encoded bph Pathway in Comamonas testosteroni (Formerly Pseudomonas sp.) NCIMB 10643

  • Kim, Jong-Soo;Kim, Ji-Hyun;Ryu, Eun-Kyeong;Kim, Jin-Kyoo;Kim, Chi-Kyung;Hwang, In-Gyu;Lee, Kyoung
    • Journal of Microbiology and Biotechnology
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    • v.14 no.2
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    • pp.302-311
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    • 2004
  • Comamonas testosteroni (formerly Pseudomonas sp.) NCIMB 10643 can grow on biphenyl and alkylbenzenes $(C_2-C_7)$ via 3-substituted catechols. Thus, to identify the genes encoding the degradation, transposon-mutagenesis was carried out using pAG408, a promoter-probe mini-transposon with a green fluorescent protein (GFP), as a reporter. A mutant, NT-1, which was unable to grow on alkylbenzenes and biphenyl, accumulated catechols and exhibited an enhanced expression of GFP upon exposure to these substrates, indicating that the gfp had been inserted in a gene encoding a broad substrate range catechol 2,3-dioxygenase. The genes (2,826 bp) flanking the gfp cloned from an SphI-digested fragment contained three complete open reading frames that were designated bphCDorfl. The deduced amino acid sequences of bphCDorfl were identical to 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC), 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), and OrfI, respectively, that are all involved in the degradation of biphenyl/4-chlorobiphenyl (bph) by Ralstonia oxalatica A5. The deduced amino acid sequence of the orfl revealed a similarity to those of outer membrane proteins belonging to the OmpW family. The introduction of the bphCDorfl genes enabled the NT-l mutant to grow on aromatic hydrocarbons. In addition, PCR analysis indicated that the DNA sequence and gene organization of the bph operon were closely related to those in the bph operon from Tn4371 identified in strain A5. Furthermore, strain A5 was also able to grow on a similar set of alkylbenzenes as strain NCIMB 10643, demonstrating that, among the identified aromatic hydrocarbon degradation pathways, the bph degradation pathway related to Tn4371 was the most versatile in catabolizing a variety of aromatic hydrocarbons of mono- and bicyclic benzenes.