• Title/Summary/Keyword: B16 mouse melanoma cells

Search Result 25, Processing Time 0.486 seconds

The Effects of Areca catechu Extracts on Tyrosinase Gene Expression in B16 Mouse Melanoma Cells (B16 마우스 흑색 종양 세포에서 빈랑(Areca catechu) 추출물이 티로시나아제 유전자에 미치는 효과)

  • Chin, Jong-Eon;Cho, Nam-Chul
    • The Korean Journal of Food And Nutrition
    • /
    • v.20 no.2
    • /
    • pp.240-244
    • /
    • 2007
  • In this study, we found that the methanolic extract of Areca catechu repressed expression of the tyrosinase gene in B16 mouse melanoma cells containing a tyrosinase promoter. Extract concentrations of 100 ${\mu}$g/ml and 500 ${\mu}$g/ml exhibited tyrosinase gene expression rates of aproximately 62% and 48%, respectively, compared to the control. The fraction layers consisting of ethyl acetate, butyl alcohol, and water showed repressive effects on the tyrosinase gene. In particular, the butyl alcohol fraction highly repressed at 100 ${\mu}$g/ml and 500 ${\mu}$g/ml. In the MTT assay, the methanolic extracts exhibited very low cytotoxicities at 1 ${\mu}$g/ml, 10 ${\mu}$g/ml, and 100 ${\mu}$g/ml.

Inhibitory effects of some medicinal plant extracts on the tyrosinase promoter activity on B16 mouse melanoma cells

  • Chin, Jong-Eon;Sun, Heung-Suk;Lee, Kwang-Jae;Choi, Tae-Jin;Ko, Yoo-Seung;Sohn, Hyun-Jung;Kim, Jeong-Joong;Jeon, Byung-Hoon;Blaise Lee, Hwang-Hee
    • Advances in Traditional Medicine
    • /
    • v.1 no.2
    • /
    • pp.6-13
    • /
    • 2000
  • Melanin is specifically produced in melanocytes. The pathway for melanin biosynthesis is mainly controlled by tyrosinase. To estimate the inhibitory effect of melanin biosynthesis from 31 medicinal plants extracts, we tested the inhibitory effects of the tyrosinase promoter on B16 mouse melanoma cells. The result of this study demonstrated that Mori Radicis Cortex and Castena Fractus extracts only in tested medicinal plant extracts have high inhibitory effects on tyrosinase promoters with very low cytotoxicity on B16 mouse melanoma cells. Therefore, extracts of Mori Radicis Cortex and Castena Fractus were evaluated as very effective negative regulators of tyrosinase gene expression.

  • PDF

Depigmentation Activity of Barley, Unpolished Rice, Job's-tear (보리·현미·율무의 미백효과에 관한 연구)

  • Lee, Tae-huen;Kim, Hye-jeong;Kim, Yoon-bum
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
    • /
    • v.16 no.2
    • /
    • pp.57-77
    • /
    • 2003
  • Objectives: The aim of this study was to investigate the depigmentation effects of Barley, Unpolished rice, Job's-tear. Metbods: We investigated that the extracts of Barley, Unpolished rice, Job's-tear inhibit activity of tyrosinase, the enzyme which convert ts 3-(3,4-dihydroxyphenyl) alanine to dopachrom in the biosynthetic process of melanin, the UV absorbance of the extracts in the UV-A region and UV-B region was measured by UV scanning, the effect of extracts on cell viability and melanin production in cultured B16 mouse melanoma cells were measured, and cytoprotective effects of extracts on PC12 cells injured by hydrogen peroxide measured by MTT assay. Results: The extracts of Barley, Unpolished rice, Job's-tear inhibited activity of tyrosinase in low density. The Barley, Job's-tear extracts not only showed inhibitory effects on melanin production in cultured B16 mouse melanoma cells, but also exhibited cytoprotective effects on PC12 cells injured by hydrogen peroxide in low density. Unpolished rice extract showed inhibitory effect on melanin production in cultured B16 mouse melanoma cells, but did not showed cytoprotective effect Barley, Unpolished rice, Job's-tear extracts did not showed an absorbance effect in the UV-A region and UV-8 region. Conclusions: These results suggest that Barley, Unpolished rice, Job's-tear inhibit melanin biosynthesis which is involved in hyperpigmentation and could be used as a whitening agent.

  • PDF

Inhibitory Effect and Mechanism on Melanogenesis of Radix glycyrrhizae Water Extract (감초 물추출물의 멜라닌 형성 억제효과 및 기전에 관한 연구)

  • Kim, Jin;Kwen, Il-ho;Lim, Hong-jin;Lim, Kyu-sang;Hwang, Chung-yeon
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
    • /
    • v.16 no.2
    • /
    • pp.96-118
    • /
    • 2003
  • The effect of Glycyrrhizae Radix water extract, known as depigmenting agent, on melanin biosynthesis was investigated in cellular level by using B16 mouse melanoma cells. The inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis was determined by mushroom tyrosinase assay traditionally using in vitro screening test. To determine whether Glycyrrhizae Radix water extract suppress melanin synthesis in cellular level, B16 mouse melanoma cells were cultured in the presence of different concentrations of Glycyrrhizae Radix water extract. Effects on cell proliferation, melanin biosynthesis, tyrosinase activity, DOPAchrome tautomerase activity, and expression level of mRNA for tyrosinase were examined. The maximum concentration of Glycyrrhizae Radix water extract that was not inhibitory to growth of the cells was 2 mgml. At that concentration, melanin synthesis was significantly inhibited without cytotoxicity after 5 days, compared with untreated cells. The treatment with Glycyrrhizae Radix water extract reduced tyrosinase and DOPAchrome tautomerase activity in a dose-dependent manner. However, the treatment with Glycyrrhizae Radix water extract did not affect significantly mRNA levels for tyrosinase. These results suggest that the inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis is correlated with the suppression of tyrosinase and DOPAchrome tautomerase activity more than altering mRNA levels of tyrosinase.

  • PDF

Inhibitory Effect of Lichen Metabolites and their Synthetic Analogues on Melanin Biosynthesis in Cultured B-16 Mouse Melanoma Cells

  • Matubara, H.;Miharu, K.;Kinoshita, K.;Koyama, K.;Ye, Yang;Takahashi, K.;Yoshimura, I.;Yamamoto, Y.;Miura, Y.;Kinoshita, Y.
    • Natural Product Sciences
    • /
    • v.4 no.3
    • /
    • pp.161-169
    • /
    • 1998
  • The analogues of lichen components showing anti-tyrosinase activities were synthesized. 4-Alkylresorcinol derivatives showed both the inhibitory activity and cytotoxicity in B-16 melanoma cells at the doses of 10 mM to 1.2 mM. Resorcinol and 4-methylresorcinol showed the inhibitory effect with a low cytotoxicity at the doses of 2.5 mM and $600\;{\mu}M$ among 4-alkylresorcinols, respectively. Some diphenylmethane derivatives (Type A, B, and C) had strong activities with a low cytotoxicity. While xanthine derivatives had no effect. Glucosides of 4,5-alkylresorcinol and the diphenylmethane derivative (Type B) were prepared to decrease the cytotoxicity. As a result, no effect were observed. Liposome of the diphenylmethane derivative (Type B) was prepared for the same purpose, and the latter showed a remarkable effect at the dose of $15\;{\mu}M$ with a low cytotoxicity.

  • PDF

Depigmentation activity of Kakamseosiokyong-san (가감서시옥용산의 미백효과에 관한 연구)

  • Son, Dong-Seok;Kim, Yoon-Bum
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
    • /
    • v.15 no.2
    • /
    • pp.104-117
    • /
    • 2002
  • Objective : The aim of this study was to investigate the skin-whitening effect of Kakamseosiokyong-san Method : We investigated that the extracts of Kakamseosiokyong-san inhibit activity of tyrosinase, the enzyme which converts 3-(3,4-dihydroxyphenyl)alanine to dopachrom in the biosynthetic process of melanin. the UV absorbance of the extracts in the UV - A region and UV - B region was measured by UV scanning. the effect of extracts on cell viability and melanin production in cultured B16 mouse melanoma cells was measured, and cytoprotective effects of extracts on PC12 cells injured by hydrogen peroxide was measured by MTT assay Results: The extracts of Kakamseosiokyong-san inhibited activity of tyrosinase. The extracts not only showed inhibitory effects on melanin production in cultured B16 mouse melanoma cells, but also exhibited cytoprotective effects on PC12 cells injured by hydrogen peroxide, but did not showed an absorbance in the UV - A region and UV - B region. Conclusion: These results suggest that Kakamseosiokyong-san inhibit melanin biosynthesis which is involved in hyperpigmentation and could be used as a whitening agent for the skin.

  • PDF

Melanin Biosynthesis Inhibitors from the Tubers of Gastrodia elata

  • Li Gao;Kim, Jae-Hyon;Xu, Ming-Lu;Seo, Chang-Seob;Kim, Hyo-Jin;Lee, You-Jeong;Lee, Yeun-Koung;Lee, Seung-Ho;Chang, Hyeun-Wook;Son, Jong-Keun
    • Proceedings of the PSK Conference
    • /
    • /
    • pp.206.3-206
    • /
    • 2003
  • The bioassay-guided fractionation of the methylene chloride soluble portion of a methanol extract of Gastrodia elata tubers led to the isolation of a new furfural, 5-(4-hydroxy-benzyloxymethyl)-furan-2-carbaldehyde (2), together with four known compounds (1, 3-5), which exhibited potent inhibitory activity at the concentration of 25 $\mu\textrm{g}$/ml on melanin biosynthesis in cultured B-16 mouse melanoma cells.

  • PDF

Effect of the Aqueous Extract of Astragalus membranaceous BUNGE on Melanin Formation in B16 Mouse Melanoma Cell Line (황기 물추출물이 B16F10 Mouse Melanoma세포의 멜라닌 생성에 미치는 영향)

  • Kim, Young-Ock;Lee, Eun-Mi;Ahn, Duk-Kyun;Shin, Jun-Sik;Lee, Sung-Whan
    • Korean Journal of Korean Medical Institute of Dermatology and Aesthetics
    • /
    • v.1 no.1
    • /
    • pp.5-15
    • /
    • 2005
  • The purpose of this study was to investigate the effect of the aqueous extract of the Astragalus membranaceous(AM). AM showed inhibitory effect on the tyrosinase activity using L-tyrosine as a substrate. Tyrosinase plays an important role in the process of melanin polymer biosynthesis. In vitro AM extract(1mg/ml) inhibited melanin biosynthesis and are useful for the material used in cosmetics. B16 mouse melanoma cells were cultured in different concentrations. The non-cytotoxicity of the plant extracts was confirmed by MTT assay.

  • PDF

Inhibitory Effects of Hydrolysable Tannins on Tyrosinase Activities in B16 Mouse Melanoma Cells

  • Cho, Soo-Min;Kwon, Young-Min;Lee, Jae-Hee;Lee, Min-Won
    • Natural Product Sciences
    • /
    • v.8 no.4
    • /
    • pp.183-185
    • /
    • 2002
  • To investigate skin whitening natural substances, the effects on melanogenesis by measuring the tyrosinase activity and the melanin contents of three hydrolysable tannins, $1,2,6-tri-O-galloyl-{\beta}-D-glucose$ (1), 2,3-(S)-HHDP-D-glucose (2) and pedunculagin (3) in B16 melanoma cells were examined. $1,2,6-Tri-O-galloyl-{\beta}-D-glucose$ (1), 2,3-(S)-HHDP-D-glucose (2) and pedunculagin (3) inhibited tyrosinase activity in B16 melanoma cells in a dose-dependent manner.

The Effects of Bamboo Extract on Human Melanocytes and B16 Melanoma Cells in vitro

  • Cho, Joon-Hwan;Lee, Ki-Moo;Kim, Nam-Soo;Kang, Won-Hyoung
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.25 no.4
    • /
    • pp.133-143
    • /
    • 1999
  • To identify inhibitory effect of Bamboo extract on melanogenesis, the effect was compared with arbutin, ascorbic acid, hydroquinone, and kojic acid on the melanin biosynthesis in B16 mouse melanoma cells and cultured human melanocytes. The cell viability of the agent was tested on cultured human melanocytes. We also examined its. free radical scavenging activity on 1,1-diphenyl-2-picrylhydrazyl(DPPH). Bamboo extract showed considerable effect against melanin production and did not reduce cell viability at the concentration tested. It also showed potent free radical scavenging activity.

  • PDF