• Title, Summary, Keyword: Angelica dahurica

Search Result 77, Processing Time 0.031 seconds

Development of Chloroplast DNA-Based Simple Sequence Repeat Markers for Angelica Species Differentiation (당귀 종판별을 위한 엽록체 기반 SSR 마커 개발)

  • Park, Sang Ik;Kim, Serim;Gil, Jinsu;Lee, Yi;Kim, Ho Bang;Lee, Jung Ho;Kim, Seong Cheol;Jung, Chan Sik;Um, Yurry
    • Korean Journal of Medicinal Crop Science
    • /
    • v.24 no.4
    • /
    • pp.317-322
    • /
    • 2016
  • Background: In the herbal medicine market, Angelica gigas, Angelica sinensis, and Angelica acutiloba are all called "Danggui" and used confusingly. We aimed to assess the genetic diversity and relationships among 14 Angelica species collected from different global seed companies. Toward this aim we developed DNA markers to differentiate the Angelica species. Methods and Results: A total of 14 Angelica species, A. gigas, A. acutiloba, A. sinensis, A. pachycarpa, A. hendersonii, A. arguta, A. keiskei, A. atropurpurea, A. dahurica, A. genuflexa, A. tenuissima, A. archangelica, A. taiwaniana, and A. hispanica were collected. The genetic diversity of all 14 species was analyzed by using five chloroplast DNA-based simple sequence repeat (SSR) markers and employing the DNA fragment analysis method. Each primer amplified 3 - 12 bands, with an average of 6.6 bands. Based on the genetic diversity analysis, these species were classified into specific species groups. The cluster dendrogram showed that the similarity coefficients ranged from 0.77 to 1.00. Conclusions: These findings could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Angelica species. The analysis of polymorphic SSRs could provide an important experimental tool for examining a range of issues in plant genetics.

Basic Research of Development for Food of Seedling in Some Plants (몇가지 식물에 있어서 어린순의 식품학적 가치개발을 위한 기초연구)

  • 박석근
    • Korean Journal of Plant Resources
    • /
    • v.8 no.3
    • /
    • pp.335-339
    • /
    • 1995
  • This experiments were carried out to develop for food in some plants seeding. Plant materials were Raphanus sativus, Perilla frutescens var. acuta, Perilla frutescens var. japonica, Scutellaria baicalensis, Angelica dahurica, Angelica gigas, Ligusticum acutilobum, Peucedanum japonicum, Platycodon grandiflorum, Codonopsis lanceolata, Allium monanthum and Fagopyrum esculentum. The results obtained are summarized as follows ; germination rate was the highest in Raphanus sativus and germination vigor was excellent in Perilla frutescens var. acuta Total overall acceptability was the best in Perilla frutescens var. acuta

  • PDF

Morphological Characteristics of Seeds in Medicinal Plants of Umbelliferae (산형과 약용작물 종자의 형태적 특성)

  • Eun Il, Lee;Young Ok, Ko;Chu Ho, Choi;Jong Ki, Lee;Seok Hyeon, Kim
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.42 no.4
    • /
    • pp.429-434
    • /
    • 1997
  • The most typical morphological characteristics of seeds of eight species in Umbelliferae are summarized as follows: 1. The common characteristics of seeds in Umbelliferae were bi-partite fruits, compound of 2-seeds, (fruitlet) oil canals and funiculus or carpaphore. 2. Seed shape of Umbelliferae were oblong or elliptic and ovate; seed colors were yellow, pale yellow or brown, dark brown or black. 3. Seed size ranged from 2 to 7mm in length and 2∼5mm in width. Angelica dahurica bore the largest seed while Ligusticum acutilobum showed the smallest. The 1000-seed weight ranged from 1. 7g in Bupleurum falcatum to 3.9g in Angelica gigas.

  • PDF

Cytogenetic Analysis of Seven Angelica Species (Angelica속 식물 7종의 세포유전학적 분석)

  • Choi, Hae-Woon;Koo, Dal-Hoe;Lee, Woo-Kyu;Kim, Soo-Young;Sung, Jung-Sook;Seong, Nak-Sul;Suh, Young-Bae;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
    • /
    • v.13 no.3
    • /
    • pp.118-121
    • /
    • 2005
  • Karyotypes were established in seven Angelica species cultivated in Korea. The somatic chromosome numbers were 2n = 2x = 22 with the basic number of x = 11 in all Angelica plants examined. Their metaphase chromosomes ranged from 3.56 ${\mu}M$. to 8.91 x. in length. Distinctive Karyotypes were found in two species, A. tenuissima with all metacentries, K(2n) = 2x = 22m, and A. genuflexa with all subtelocentrics, K(2n) = 2x = 22st. Karyotype formulas of A. gigas, A. acutiloha, A. sinensis, A. decursiva and A. dahurica were K(2n) = 2x = 20m + 2sm, K(2n) = 2x = 12m + 10sm, K(2n) = 2x = 16m + 6sm, K(2n) = 2x = 18m + 4sm and K(2n) = 2x = 10m + 10sm + 2st, respectively. Cytological data showed that chromosomal polymorphisms within species were observed in Angelica plants compare to other regions.

Determination of Byakangelicin in Angelicae dahuricae Radix by High Performance Liquid Chromatography (고속액체크로마토그라피에 의한 백지근 중 Byakangelicin의 정량)

  • Shin, Kuk-Hyun;Kang, Sam-Sik;Chi, Hyung-Joon
    • Korean Journal of Pharmacognosy
    • /
    • v.21 no.3
    • /
    • pp.239-241
    • /
    • 1990
  • A new method for quantitative determination of byakangelicin in Angelicae dahuricae Radix by high performance liquid chromatography was established. A reversed-phase system with a ${\mu}Bondapak$ $C_{18}$ column using THF : dioxane : MeOH : HAc : 5% $H_3PO_4$ : $H_2O$=72.5 : 62.5 : 25 : 10 : 1 : 329 as a mobile phase was developed. Byakangelicin together with ter-O-byakangelicin and oxypeucedanin methanolate, and isooxypeucedanin as an internal reference were detected at 350 nm and the analysis was successfully carried out within 30 min.

  • PDF

Effect of the Constituents of Angelicae dahuricae Radix on Hepatic Drug Metabolizing Enzyme Activity (백지근(白芷根) 성분(成分)이 간(肝)의 약물대사효소활성(藥物代謝酵素活性)에 미치는 효과(效果))

  • Shin, Kuk-Hyun;Kim, Ok-Nam;Woo, Won-Sick
    • Korean Journal of Pharmacognosy
    • /
    • v.19 no.1
    • /
    • pp.19-27
    • /
    • 1988
  • The hexane and ether extracts from the roots of Angelica dahurica caused a significant inhibition of hepatic drug-metabolizing enzyme (DME) activity. Through systematic fractionation by $SiO_2\;column$ and vacuum liquid chromatography monitoring by bioassays, three furanocoumarins, phellopterin, byakangelicin and tert-O-methylbyakangelicin were isolated as active principles. These components have biphasic responses, both inhibitory and inducing effects on DME system. Tert-O-methyl byakangelicin was found to have the strongest enzyme inhibitory potency.

  • PDF

Antimicrobial Activity of Medicinal Plant Extracts against Streptococcus mutans II

  • Eum, Jin-Seong;Park, Young-Doo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • /
    • pp.865-869
    • /
    • 2008
  • This study was carried out to research antimicrobial agents from medicinal plants, Pueraria thunbergiana, Sophora flavescens, Lonicera japonica, Pinellia ternata, Dictamnus dasycarpus, Paeonia lactiflora, Angelica dahurica, Spirodela polyrhiza, Cryptotympana pustulata, Pinus densiflora, Cimicifuga heracleifolia, Bupleurum falcatum, Magnolia kobus, Artemisia princeps, Houttuynia cordata, Forsythia koreana, Arctium lappa, Castanea crenata, Aster tataricus, Citrus unshiu, Gardenia jasminoides, Ledebouriella seseloides, Hovenia dulcis, Prunus sargentii. The ethanol extracts of 24 medicinal plants were tested for the antimicrobial activity against Streptococcus mutans. The extracts of Sophora flavescens, Pinus densiflora, Cimicifuga heracleifolia, Gardenia jasminoides and Ledebouriella seseloides showed antimicrobial activity against Streptococcus mutans. The extracts of Pinus densiflora and Sophora flavescens among these medical plants showed significant antimicrobial activity against Streptcoccus mutans. These results suggested that the extracts from Pinus densiflora and Sophora flavescens could be the potential source of antimicrobial agents against S. mutans.

  • PDF

Screening of Anti-atopic Herbs Having Phosphodiesterase 4 and 7 Inhibition (Phosphodiesterase 4와 7에 대한 억제 기전을 갖는 아토피 억제 물질의 탐색)

  • Yoon, Won Ho;Lee, Keyong Ho
    • Korean Journal of Pharmacognosy
    • /
    • v.45 no.3
    • /
    • pp.268-274
    • /
    • 2014
  • The aim of this study is to explore the potent phosphodiesterase 4 and 7 inhibitor from various herbal medicines for atopy treatment. In this study, 51 kinds of each herbal medicine, which were extracted with ethanol, was carried out the screening of PDE 4 and 7 inhibition using enzyme inhibitory assay. Of these, 8 species of herbal medicines, Rubus coreanus, Duchesnea chrysantha, Alisma orientale, Rehmannia glutinosa, Angelica dahurica, Thuja orientalis, Astragalus membranaceus and Perilla frutescens were screened as potential inhibitor against PDE 4 and 7. Among 8 species, Duchesnea chrysantha showed poteinial anti-atopic effect on DNCB-induced atopic model. Duchesnea chrysantha extract decreased serum IgE and histamine release significantly.

Germanium Contents of Soil and Crops in Gyeongnam Province (경남지역의 토양 및 농작물중 게르마늄 함량)

  • Lee, Seong-Tae;Lee, Young-Han;Lee, Hong-Jae;Cho, Ju-Sik;Heo, Jong-Soo
    • Korean Journal of Environmental Agriculture
    • /
    • v.24 no.1
    • /
    • pp.34-39
    • /
    • 2005
  • To investigate the germanium content in paddy soil in Gyeongnam province, 310 paddy soil samples were collected at 19 areas in Gyeongnam, Ulsan and Busan. Contents of germanium in paddy soils were analyzed in different topographies, soil types and soil textures. Average content of germanium in Gyeongnam was 0.24 mr/kg, those of Masan, Jinju and Hadong were above 0.30 mg/kg. Germanium content with different topographies were no difference. Germanium contents in different soil types were 0.27 mg/kg in well adapted soil and 0.23 mg/kg in poorly drained soil. Germanium contents in different soil textures were 0.27 mg/kg in silt loam and 0.23 mg/kg in sandy loam. To determine germanium content on agricultural product in the field, content of germanium in cereals, vegetables and fruits were analyzed. Germanium content of agricultural product was high in the order of cereals>vegetables>fruits. In case of vegetables, germanium contents were high in the order of leaf vegetables>root vegetables>fruit vegetables. Germanium contents were high with 62 and $65{\mu}g/kg$ in lettuce and young radish, respectively. To analyze the germanium content in medicinal plant, samples were collected from 19 medicinal plants at Hamyang areas. Germanium contents in Angelica keiskei, Ligusticum chuanxiong, Panax ginseng and Atractylodes macrocephala were relatively high with $100{\mu}g/kg$ above.

Topical application of herbal formula for the treatment of ligature-induced periodontitis

  • Kim, Mi Hye;Choi, You Yeon;Lee, Hye Ji;Lee, Haesu;Park, Jung-Chul;Yang, Woong Mo
    • Journal of Periodontal and Implant Science
    • /
    • v.45 no.4
    • /
    • pp.145-151
    • /
    • 2015
  • Purpose: The aim of this study was to investigate the therapeutic effects of a herbal formula, PerioH-035, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum on the periodontal breakdown in a well-established ligature-induced periodontitis model in rats. Methods: Sprague-Dawley rats were randomly assigned to 1 of 4 groups: NL (non-ligatured), L (ligatured), P1 (ligatured and treated with 1 mg/mL PerioH-035), P100 (ligatured and treated with 100 mg/mL PerioH-035). Periodontitis was induced by placing a ligature around the mandibular first molars. PerioH-035 was topically applied to both sides of the first molar for 2 weeks. The right side of the mandibles was retrieved for micro-computed tomography (CT) and methylene blue staining to analyze alveolar bone loss. The left side of the mandibles was histologically analyzed by TRAP and H&E staining. The MMP-9 mRNA level in gingival tissue was investigated by RT-PCR. Results: Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups. The number of dense multi-nucleated cells found to be TRAP-positive by staining in the ligatured rats was markedly decreased by PerioH-035 application. In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups. Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation. Conclusions: These results suggest that PerioH-035 has therapeutic effects on periodontitis, and thus, PerioH-035 shows promise as a treatment for periodontitis.