• Title/Summary/Keyword: Amino-acid

Search Result 6,357, Processing Time 0.067 seconds

Amino Acid Composition of Human and Cow's Milk (모유 및 우유의 아미노산 조성에 관한 비교연구)

  • 고영수
    • Journal of the Korean Home Economics Association
    • /
    • v.24 no.4
    • /
    • pp.51-55
    • /
    • 1986
  • The composition of human milk was compared with that of cow's milk. The contents of amino acids were analyzed by amino acid autoanalyzer. The content of glutamic acid in cow's milk was three times as much as that in human milk. The content of essential amino acid in human colostrum was twice as much as that in mature milk.

  • PDF

AMINO ACID DIGESTIBILITY TO PIGS IN VARIOUS FIBER SOURCES 2. TRUE DIGESTIBILITY OF AMINO ACIDS IN ILEAL DIGESTA AND FECES

  • Nongyao, A.;Han, In K.;Choi, Y.J.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.4 no.3
    • /
    • pp.211-218
    • /
    • 1991
  • The effects of dietary fiber on true digestibility of amino acids by growing pigs were studied, using semi-purified diets formulated from alfalfa meal, cassava leaf meal, rubber seed meal and leucacna meal at 20% level. A protein-free diet including 5% cellulose was formulated for correcting the endogenous amino acid loss. Across all the diets, arginine was the most digestible while the least at ileal level was threonine; methionine and/or histidine at fecal level respectively. The true digestibility value of amino acids at ileal level were higher than at fecal level except control diet (cellulose). The true digestibility values at ileal level were similar for all diets but differed at fecal level in different magnitude. These results indicate that undigestible compound in individual feedstuff might confound. True digestibility should be studied together for accurate diet formulation as apparent digestibility decreased when their amino acid concentration in the diet was reduced.

Prediction of Metal Ion Binding Sites in Proteins from Amino Acid Sequences by Using Simplified Amino Acid Alphabets and Random Forest Model

  • Kumar, Suresh
    • Genomics & Informatics
    • /
    • v.15 no.4
    • /
    • pp.162-169
    • /
    • 2017
  • Metal binding proteins or metallo-proteins are important for the stability of the protein and also serve as co-factors in various functions like controlling metabolism, regulating signal transport, and metal homeostasis. In structural genomics, prediction of metal binding proteins help in the selection of suitable growth medium for overexpression's studies and also help in obtaining the functional protein. Computational prediction using machine learning approach has been widely used in various fields of bioinformatics based on the fact all the information contains in amino acid sequence. In this study, random forest machine learning prediction systems were deployed with simplified amino acid for prediction of individual major metal ion binding sites like copper, calcium, cobalt, iron, magnesium, manganese, nickel, and zinc.

Terminal Amino Acid Sequences of Alkaline Amylase from Alkalophilic Bacillus sp. MB 809 and Their Homology (호알카리성 Bacillus sp. MB 809의 알카리성 아밀라제의 말단 아미노산 서열과 그 상동성)

  • Moo, Bae;Kang, Kyung
    • Korean Journal of Microbiology
    • /
    • v.31 no.2
    • /
    • pp.175-178
    • /
    • 1993
  • Alkaline .alpha.-amylase expressed in the transformant, Baciollus subtills MB809, containing alkaline amylase gene cloned from alkalophilic Bacillus sp. AL-8, was purified through for step separation processes. The purified alkaline .alpha.-amylase had molecular weight of app[roximately 59, 000 daltons on SDS-PAGE and Sephaex G-100 gel filtration. Amino acid sequence of terminal portion of the enzyme was analyzed with pure amylase eluted form the SDS-PAGE gel. N-terminal amino acid sequence of .alpha.-amylase was determined by the Edman degradation method and resulted in $NH_{2}$-ser-thr-ala-pro-ser-(ile)-lys-ala-gly-thr-(ile)-leu. For C-terminal amino acid sequencing, purified .alpha.-amylase was digested with carboxypuptidase A and B, and reverse-phase HPLC gradient elution system resulted in -thr-trp-pro-lys-COOH.

  • PDF

Effects of Amino Acid Balance of Dietary Casein and Soy Protein on the Plasma and Liver Cholesterol Levels in Young Chicks (사료중 Casein 및 대두단백질의 아미노산 균형이 병아리의 혈액 및 간조직내의 Cholesterol함량에 미치는 영향)

  • 김현숙;지규만;최인숙
    • Korean Journal of Poultry Science
    • /
    • v.22 no.1
    • /
    • pp.43-54
    • /
    • 1995
  • Effects of dietary protein sources and their amino acid compositions on the liver and plasma cholesterol levels in growing Single Comb white Leghorn male chicks were studied. A diet containing isolated soy protein (21% cp) was supplemented with 0.5% DL-methionine and 0.3% L-glycine. and another diet containing casein(21% cp) was supplemented with 1.5% L-arginine HCl, 0.4% DL-methionine. and 1.0% L-glycine. Plasma cholesterol level was markedly lower in groups force-fed the diets containing either soy protein or casein supplemented with amino acids compared to those found in birds fed corresponding diets without amino acids supplementation. The cholesterol lowering effect of the casein diet. when balanced with various supplemental amino acids appeared to be due to arginine instead of methionine or glycine. It is likely that amino acid balance rather than the composition of a specific amino acid is one of the major factors determining the effect of dietary protein sources on the blood cholesterol levels in chicks.

  • PDF

Electron Capture Dissociation Mass Spectrometry for Gaseous Protonated Melittin Ions and Its Single Amino Acid Substituted Variants

  • Yu, Seonghyun;Jang, Hwa-yong;Oh, Han Bin
    • Mass Spectrometry Letters
    • /
    • v.10 no.4
    • /
    • pp.117-122
    • /
    • 2019
  • This study demonstrated the sensitivity of electron capture dissociation mass spectrometry (ECD-MS) to probe subtle conformational changes in gaseous melittin ions induced by the substitution of an amino acid. ECD-MS was performed for triply and quadruply-protonated melittin and its variants obtained by a single amino acid substitution, namely, D-Pro14, Pro14Ala, and Leu13Ala. Although native triply-protonted melittin showed only a few peptide backbone cleavage products, the D-Pro14 and Pro14Ala variants exhibited extensive backbone fragments, suggesting the occurrence of a significant structural or conformational change induced by a single amino acid substitution at Pro14. On the contrary, the substitution at Leu13, namely Leu13Ala (+3), did not cause significant changes in the ECD backbone fragmentation pattern. Thus, the sensitivity of ECD-MS is demonstrated to be good enough to probe the aforementioned conformational change in melittin.

Chemical composition and Stabilities of Invertase from Korean Ginseng, Panax ginseng (고려인삼(Panax RiwenR) Invertase의 화학조성과 안정성)

  • 김용환;김병묵
    • Journal of Ginseng Research
    • /
    • v.14 no.1
    • /
    • pp.21-26
    • /
    • 1990
  • The chemical composition and stabilities of the purified ginseng invertase were investigated. The purified enzyme was found to be a glycoprotein composed of 80.2% protein and 19.7% total sugar. The protein component of the enzyme was composed of acidic amino acid (9.3%), basic amino acid (48.9%), nonpolar amino acid (21.4%), polar amino acid (20.4%) and 6.1% S-containing amino acid. It showed especially high contents of histidine and serine. The enzyme was inactivated almost completely by the treatment with some proteases (papain, pepsin. trypsin, pancreatin and microbial alkaline pretense) and protein denatllrants (8M urea and 6M guanidine-HC1), bolt not with glyrosidase (${\alpha}$-amylase, ${\beta}$-amylase. glcoamylese and cellullase). btonosaccharides sllch as glilrose, fructose, galactose and mannose did not exert any influence on the enzyme activity. The activity of the enzyme was inhibited by Ag+, Mn2+, Hg2+, Zn2+ and Al3+, whereas Ca2+, Mg2+, Ba2+ and Fe3+ gave rather activating effects on the enzyme activity. The enzyme was relatively stable in the VH range of VH 6 and 8, and at the temperatures below 35$^{\circ}C$.

  • PDF

Studies on the Chemical Composition of Korean Wheat Flour Proteins (II). Molecular Weight of the Main Gliadin Protein and Its Terminal Amino Acid Determination (한국산 밀가루 단백질의 화학적 조성에 관한 연구 (제2보) 주 Gliadin 단백질의 분자량 측정 및 말단아미노산의 검출)

  • Sung Hye Cho;Jun Pyong Kim
    • Journal of the Korean Chemical Society
    • /
    • v.21 no.3
    • /
    • pp.215-218
    • /
    • 1977
  • We have investigated for amino acid composition, molecular weight and terminal amino acid of the main gliadin protein which was purified by Sephadex column. The results were obtained as follows: 1. The highest content of amino acid was glutamic acid for the main gliadin protein. 2. The molecular weight was estimated 60,200 ${\pm}$200 for the main gliadin protein. 3. Identified N-terminal and C-terminal amino acid of the main gliadin protein were phenylalanine and methionine, respectively.

  • PDF

Phylogenetic study of penicillium chrysogenum based on the amino acid sequence analysis of chitin synthase

  • Park, Bum-Chan;Lee, Dong-Hun;Sook, Bae-Kyung;Park, Hee-Moon
    • Journal of Microbiology
    • /
    • v.35 no.3
    • /
    • pp.159-164
    • /
    • 1997
  • The phylogenetic study of Penicilium chrysogenum was performed based on amino acid sequence comparison of chitin synthase. Phylogenetic trees were constructed with the deduced amino acid sequences of the highly conserved region of chitin synthease gene fragments amplified by PCR. The BlasP similarity searcch and the bootstrap analysis of the deduced amino acid sequences of chitin synthase from P. chrysogenum with those form other fungi showed a close evolutionary relationship of Penicillium to ascomycetous fungi, especially to genus Aspergilus. The result from bootstrap analysis of the deduced amino acid sequences of the Class II chitin synthase from ascomyceteous fungi supported the usefulness of the Class II chitin synthease for phylogenetic study of filamentous fungi.

  • PDF

Screening and Taxonomic Charactrization of D-Amino Acid Aminotransferase-producing Thermophiles (D-Amino Acid Aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구)

  • 곽미선;이승구;정상철;서승현;이재흥;전영중;김영호;성문희
    • Microbiology and Biotechnology Letters
    • /
    • v.27 no.3
    • /
    • pp.184-190
    • /
    • 1999
  • To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acid aminotransferase (D-AAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two thermophiles, Bacillus sp. Lk-1 and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-01, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.

  • PDF