• Title, Summary, Keyword: Amino-acid

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Studies on the Changes in Amino acid of Soybean (irradiated) during Fermentation (대두(조사)의 발효에 의한 Amino acid의 변화에 관한 연구)

  • Hur yun Haeng
    • Journal of environmental and Sanitary engineering
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    • v.2 no.3
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    • pp.13-22
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    • 1987
  • For the amino-N -ratio and N- solubity, irradiated samples were higher than non - irradiated them, that is, 15KGy(l9, 72), 7KGy(l9, 55) of amino-N-ratio were more those of 5KGy(17. 91) and l0KGy(l8, 06). during the fermentation of samples. For the Solubility of nitrogen, irradiated samples were decreased sequencely, such as 10KGy, 7KGy, 15KGy and 5KGy. For the amounts of amino acid, Content of the glutamic acid was the highest in the all samples and the main free amino acids in the Steamed Soybeans Were glutamic acid, proline, arginine, and alanine etc. However the amount of amino acid in the irradiated Sample was notably increased in Comparison with the nonirradiated $(10.8017\%)$ and 7KGy amino acid $(15.4166\%)$ was highest.

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Liquid Chromatographic Enantiomer Separation of α-Amino Acid Esters as Nitrobenzoxadiazole Derivatives Using Polysaccharide-Derived Chiral Stationary Phases

  • Islam, Md. Fokhrul;Lee, Wonjae
    • Journal of the Chosun Natural Science
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    • v.8 no.2
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    • pp.111-116
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    • 2015
  • Liquid chromatographic enantiomer separation of ${\alpha}$-amino acid esters as nitrobenzoxadiazole (NBD) derivatives was performed using several chiral stationary phases (CSPs) based on polysaccharide derivatives under fluorescence detection. For enantiomer separation by normal HPLC, the non-aqueous derivatization method of ${\alpha}$-amino acid esters for NBD analytes was introduced. Among the six CSPs used in this study, the performance of Chiralpak IA was superior for enantiomer resolution of NBD derivatives of several ${\alpha}$-amino acid methyl esters. Also the convenient analytical method using polysaccharide-derived CSPs developed in this study was applied to determine the optical purity of ${\alpha}$-amino acids esters. It was investigated that the enantiomeric impurity levels of 0.02-1.73% were found after determination of enantiomeric purities of several commercially available L-amino acid methyl esters. It is expected to be quite useful for enantiomer separation of other ${\alpha}$-amino acid esters as NBD derivatives by normal HPLC.

Studies on the Drug Development of Coicis Semen(I) -Aminoacid Contents in Coicis Semen- (의이인(薏苡仁)(Coicis Semen)의 의약품개발(醫藥品開發)에 관(關)한 연구(硏究)(I) -아미노산(酸)의 함량(含量)에 대하여-)

  • Yong, Jae-Ick
    • Journal of Pharmaceutical Investigation
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    • v.7 no.1_4
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    • pp.1-12
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    • 1977
  • Free amino acid in ethanol extracts and total amino acid hydrolysates of Coicis semen were analyzed by amino acid autoanalyzer. The sample A (unpolished Coicis Semen) and sample B(polished Coicis Semen) are used in this experiments. The results obtained from this study are as follows: 1) 17 kinds of free amino acid (Asp, Thr, Ser, Glu, Pro, Gly, Ala, Val, Cys, Met, Ileu, Lew, Try, Phe, Lys, His, Arg,) including 7 kinds of essential amino acid (Val, Lew, Ileu, Thr, Lys, Met, Phe,) as human nutrition were identified and quantified but tryptophan. 2) Total free amino acids of sample A is more than about 3 folds that of sample B. 3) The distribution of free amino acids contained in sample A, threonine is the richiest and then comes Ala, Glu, Asp, and Pro, in that order. In sample B, glutamic acid is the richiest and then comes Thr, Asp, Ala, and Gly, in that order. 4) 17 kinds of total amino acid (Asp, Thr, Ser, Glu, Pro, Gly, Ala, Val, Cys, Met, Ileu, Lew, Tyr, Pher, Lys, including 7 kinds of essential amino acid (Val, Leu, Ileu, Thr, Lys, Met, Phe,) in human nutrition except tryptophan were identified and quanified. 5) Total amino acid content of sample A is more than about 1.06 folds that of sample B. 6) Total amino acid content of sample A in acid hydrolysates is more than about 1.06 folds that of sample B in acid hydrolysates. 7) Unknown chromatogram of ethanol extracts and acid hydrolysates of Coicis Semen were identified as Ornitine.

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Comparison of the Composition of Free Sugars , Amino Acids and Minerals in Black Omija (Schizandra nigra Max) (흑오미자의 유리당, 아미노산 및 무기질 조성)

  • 신수철
    • Korean Journal of Plant Resources
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    • v.11 no.1
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    • pp.47-50
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    • 1998
  • For the quantitiative determination of chemical and taste components in black omija(Schizandra nigra Max) and omija(S.chinensis), compositions of free sugars, free amino acids, total amino acids, and minerals were analyzed. Among the total free sugars in black omija and omija, glucose and frutose were major free sugars and sucrose was little amount. The most abundant free amino acid in black imija was histidine and that in imija was serine. The major free amino acid in black omija and omija were histidine, serine, glutamic acid and aspartic acid. The major total amino acids in black omija and omija were glutamic acid, arginine , leucine and histidine. The limiting amino acid of each omija was S-containing amino acids. The abuntdant minerals in black omija and omija were K and Ca.

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Influence of the Structural Characteristics of Amino Acids on Direct Methylation Behaviors by TMAH in Pyrolysis

  • Choi, Sung-Seen;Ko, Ji-Eun
    • Bulletin of the Korean Chemical Society
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    • v.30 no.11
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    • pp.2542-2548
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    • 2009
  • Direct methylation behaviors of 20 amino acids with tetramethylammonium hydroxide (TMAH) were studied under diluted conditions with silica. Amino acid concentration was controlled by dilution with silica ($SiO_2$) and the molar ratios of amino acid/silica were 0.20, 0.50, and 2.0. The molar ratios of amino acid/TMAH (0.51 - 4.64) also varied. It was found that arginine, asparagine, aspartic acid, cysteine, glutamic acid, and glutamine did not generate any directly methylated pyrolysis products, whereas alanine, glycine, isoleucine, leucine, methionine, phenylanaline, valine, and proline generated all the directly methylated pyrolysis products. Tri- and tetra methylated products of lysine consisted of two types. Histidine and threonine hardly generated the partly methylated products. Mono- and dimethylated products of serine, tryptophan, and tyrosine were not observed. Relative intensities of the methylated products varied with the amino acid concentration, TMAH concentration, and pyrolysis temperature. Direct methylation behaviors of amino acids were explained by the structural characteristics of amino acids.

DETERMINATION OF AMINO ACIDS IN RICE(PALDAL) BY IONEXCHANGE CHROMATOGRAPHY

  • Lee, Chun-Yung;Lee, Tae-Yung;Kwon, Tai-Wan
    • Applied Biological Chemistry
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    • v.2
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    • pp.41-44
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    • 1961
  • The amino acid analysis of ground rice and the crude protein isolated from rice were performed by the most recent method of Moore, Spackman and Stein, using Amberlite IR-120. The effect of carbohydrates on the hydrolysis of rice was found to be definite in losing several amino acids nevertheless Dustin's claim that large excess of hydrolyzing acid reduces the losses of amino acid. Consequantly the removal carbohydrates as much as possible is considered to facilitate the amino acid determination. The amino acid composition of the crude rotein of rice was very similar to that previously found by other investigators.

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A Study on the Amino Acid Contents of Edible Mushrooms (식용(食用)버섯류(類)의 아미노산(酸)에 관(關)한 연구(硏究))

  • Pyo, Myoung-Yun;Ro, Ihl-Hyeob
    • Journal of Nutrition and Health
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    • v.8 no.1
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    • pp.47-59
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    • 1975
  • Free amino acids in extracts and total amino acids in hydrolysates of eleven species of edible mushrooms were analyzed by amino acid autoanalyzer (Technicon PNC-1 Type). All these 11 species of mushroom can be repesented for convenience sake as follows. S-1; Agaricus campestris Fr. S-2: Agaricus campestris S-3; Pholiota nameko(I. Ito) S. Ito et Imai S-4; Auricularia auricula-judae(Fr.) $Qu{\acute{e}}l$ S-5; Tremella fuciformis Berk. S-6; Tricholoma matsutake(S. Ito et Imai) Sing. S-7; Pleurotus ostreatus Fr. $Qu{\acute{e}}l$ S-8; Lentinus edodes Berk Sing. S-9; Ramaria botrytis (Pers.) Ricken S-10; Coprinus comatus(Fr.) S.F, Gray S-11; Gyrophora esculenta The results obtained from this study are as follows. 1) 17 kinds of amino acid, including 7 kinds of essential amino acid in human nutrition except tryptophan were identified and quantified. 2) Of all free amino acids contained in mushrooms, glutamic acid is the richest, and then comes Ala, Thr, Pro and Lys in that order. There were no found Cys'and His in S-9;His in S-1; Met and Arg in S-11; Cys and Met in S-5;Pro, Cys, Met, Lys and Arg in S-4. Of all total amino acids which are closely related with nutritional valuation, glutamic acid is the richest, and then comes Asp, Ala, Arg, Leu, Thr, Gly in that order. Especially S-1 and S-2 contain high quantity $o{\acute{i}}$ proline in both free and total amino acids. 3) Cotents of ammonia in extracts of mushrooms in decreasing order in S-1, S-10, S-8, S-2, S-7, S-6, and S-2, S-6, S-8, S-9, S-1 in hydrolysates of mushrooms. 4) Gross Contents of free amino acid in extracts is high in decreasing order in S-10, S-1, S-7, S-6, S-8, and total amino acid in hydrolysates is high in S-10, S-2, S-2, S-8, S-1, S-9, S-6. 5) Besides 17 kinds of amino acid, 5 kinds of unknown amino acid are found in extracts and hydrolysates.

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Studies on the Cellular Metabolism in Microorganisms as Influenced by Gamma-irradiation.(III) On the Changes of Protein content and Free Amino acid Pool in Yeast cells irradiated by $\gamma$-ray. (미생물의 세포생리에 미치는 전이방사선의 영향에 관한 연구(제 3보) - 효모균의 단백질함량 및 Free Amino acid Pool 에 대한 $\gamma$-ray 의 영향)

  • 김종협
    • Korean Journal of Microbiology
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    • v.5 no.2
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    • pp.79-85
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    • 1967
  • Kim, Jong Hyup., (Div. of Biology, Atomic Energy Research Institute,Korea.;) Studies on the Cellulor Metabolism in Microorganisms as influenced by Gamma-irradiation(III): On the Changes of Free Amino acid Pool and content of Protein in Yeast clls irradiated by .gamma.-ray. 1. The strain of Saccharomyces cerevisiae had been cultured synchronously in aerobic condition and irradiatel by gamma-ray from the source of cobalt-60. Drying in vacuum oven at $90^{\circ}C$ C over 12 hours, then changes of protein content (Kjeldahl) and free amino acid pool have been assayed with use of spectrophotometer. Results obtained were compared with those of unirradiated normal cells. 2. It is proved that amount of protein content in the irradiated cells increases to seven percent more than those of normal cells in the same weight of dried samples. It seems like carbohydrate breakown had been stimulated by irradiation and that relative contents of protein shows higher values than those of normal in the same weight of samples. 3. The amount of free amino acid pool in the irradiated cells shows less value about ten percent than those of normal cells, and rate of decreasing is also weak than those of standard reagent solution of amino acid. We may assume that free amino acid pool would be protected against radiation damage in living cells and more stable than in vitro. 4. The component of free amino acid pool have been assayed on second dimensional paper chromatogram, and the identified amino acids are as follows; aspartic acid, serine, glutamic acid, cystine, lysine, glycine, threonine, histidine, arginine, tyrosine, phenylalanine, valine and leucine. 5. Distributional presence of free amino acids are identical to that of normal cells except arginine, it is cosumable that radiation effect is univerlsal to all amino acid. However it is obvious that there are differences in radiolabilities of amino acids in irradiated cells.

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The Effect of Silk Amino Acid Supplementation on the Level of Blood Energy Substrates and Hormones during Prolonged Exercise

  • Zhang Seok-Am;Lee Nam-Hee;Kim Yong-Hwan
    • Proceedings of the Korean Society of Food Science and Nutrition Conference
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    • pp.171-184
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    • 2004
  • The silk amino acid supplementation is unknown to affect the release of several hormones related to energy production and metabolism during prolonged exercise. This study examined the effects of silk amino acid supplementation on the level of blood amino acid, energy substrates and hormones level during prolonged treadmill exercise in college taekwondo player. A prolonged treadmill test was carried out 60 min at 65% of maximal heart rate on 8 athletics. Blood samples were obtained form antecubital vein of subjects at rest bed 30 minute before test, after exercise and rest 1 hour. The subjects were supplemented silk amino acid (6,390 mg/day) fur 4 week. The silk amino acid supplementation did not produce significant changes on the levels of blood lactate, ammonia, amino acid, glucose, triglyceride, total cholesterol, HDL, LDL, seratonin and leptin at rest bed 30 minute before test, after exercise and rest 30 minute. The silk amino acid 4 week supplementation did not affect the levels of blood amino acid, energy substrates and hormones during prolonged treadmill exercise.

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Studies on Contents of Amino Acids in Citrus Junos Sieb (유자중(柚子中) Amino Acids에 관(關)한 연구(硏究))

  • Chung, J.H.
    • Applied Biological Chemistry
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    • v.15 no.2
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    • pp.175-180
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    • 1972
  • The chemical composition of amino acids in the rind and fresh of Citrus Junos Sieb was studied and compared with that of Citrus natsudaidai Hayate. The results were summarized as follows. 1. Beth of them contained twenty kinds of amino acids, including three kinds of unknown amino acids. 2. Proline in the rind of Citrus Junos and aspartic acid in the rind of Citrus natsudaidai were the richest of all amino acids but on the contrary. Histidine was the poorest of all amino acids in the rind of them. The content of proline amounted to 16.48 mg/100mg in the rind of Citrus Junos and the content of aspartic acid amounted to 32.18 mg/100mg in the rind of Citrus natsudaidai. 3. Aspartic acid was the richest of all amino acids in the flesh of Citrus Junos and the content of it amounted to 32.68mg/100mg. On the other hand, Proline was the richest of all amino acids in the flesh of Citrus natsudaidai and the content of it amounted to 20.93mg/100mg. But the content of histidine as 1.32 mg/100 mg in the flesh of former and tyrosine as 1.18 mg/100 mg in the flesh of latter were relatively small. 4. In the fruits of Citrus Junos and Citrus natsudaidai, aspartic acid and Proline were rich and histidine was poor in quantity. Generally, Flesh contained more amounts of all kinds of amino acids than those rind and especially glutamic acid was richer, compared with other amino acids in flesh.

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