• Title, Summary, Keyword: Air lift bioreactor

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Exopolysaccharide Production and Mycelial Growth in an Air-Lift Bioreactor Using Fomitopsis pinicola

  • Choi, Du-Bok;Maeng, Jeung-Moo;Ding, Ji-Lu;Cha, Wol-Suk
    • Journal of Microbiology and Biotechnology
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    • v.17 no.8
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    • pp.1369-1378
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    • 2007
  • For effective exopolysaccharide production and mycelial growth by a liquid culture of Fomitopsis pinicola in an air-lift bioreactor, the culture temperature, pH, carbon source, nitrogen source, and mineral source were initially investigated in a flask. The optimal temperature and pH for mycelial growth and exopolysaccharide production were $25^{\circ}C$ and 6.0, respectively. Among the various carbon sources tested, glucose was found to be the most suitable carbon source. In particular, the maximum mycelial growth and exopolysaccharide production were achieved in 4% glucose. The best nitrogen sources were yeast extract and malt extract. The optimal concentrations of yeast extract and malt extract were 0.5 and 0.1%, respectively. $K_2HPO_4\;and\;MgSO_4{\cdot}7H_2O$ were found to be the best mineral sources for mycelial growth and exopolysaccharide production. In order to investigate the effect of aeration on mycelial growth and exopolysaccharide production in an air-lift bioreactor, various aerations were tested for 8 days. The maximum mycelial growth and exopolysaccharide production were 7.9 g/l and 2.6 g/l, respectively, at 1.5 vvm of aeration. In addition, a batch culture in an air-lift bioreactor was carried out for 11 days under the optimal conditions. The maximum mycelial growth was 10.4 g/l, which was approximately 1.7-fold higher than that of basal medium. The exopolysaccharide production was increased with increased culture time. The maximum concentration of exopolysaccharide was 4.4 g/l, which was about 3.3-fold higher than that of basal medium. These results indicate that exopolysaccharide production increased in parallel with the growth of mycelium, and also show that product formation is associated with mycelial growth. The developed model in an air-lift bioreactor showed good agreement with experimental data and simulated results on mycelial growth and exopolysaccharide production in the culture of F. pinicola.

Citric Acid Production by Succharomycopsis lipolytica in Air-lift and Membrane Recycle Bioreactors (기포탑 및 막 재순환 생물반응기에서의 Saccharomycopsis lipolytica에 의한 구연산 생산)

  • 조대철;정봉현;장호남
    • Microbiology and Biotechnology Letters
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    • v.17 no.6
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    • pp.624-628
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    • 1989
  • A study on the citric acid production using Saccharomycopsis lipolytica (NRRL Y7576) was carried out in shake-flasks, air-lift and membrane recycle bioreactors. The cells entrapped in Ca-alginate beads were used in shake-flasks and air-lift reactor. Repeated batch fermentation in shake-flasks was successfully performed for 34 days and resulted in a yield of 54%. Increased yield (63%) was obtained in the air-lift reactor operation using nitrogen deficient medium (NDM). In the membrane recycle bioreactor operation, the maximal dry cell mass concentration was 39 g/1 at a dilution rate of 0.02 h$^{-1}$ and the yield with NDM was higher than that with growth medium. In addition, the yield and volumetric productivity with pure oxygen supply were greatly improved compared with those with air supply.

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Effects of Various Bioreactors on Growth and Ginsenoside Accumulation in Ginseng Adventitious Root Cultures(Panax ginseng C.A. Meyer) (다양한 생물반응기 형태가 인삼(Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 생산에 미치는 영향)

  • Kim, Yun-Soo;Hahn, Eun-Joo;Paek, Kee-Yoeup
    • Journal of Plant Biotechnology
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    • v.31 no.3
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    • pp.249-253
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    • 2004
  • The type of air lift bioreactor affected the root growth in ginseng adventitious root cultures. Among bioreactors used in this experiment, bulb type bubble bioreactor (BU) was the best to increase root growth (41.92 g dry weight). The kLa value representing the oxygen transfer capacity from medium to explants (6.98 h$^{-1}$ ) in BU with 5 cm bubble column was higher than other bioreactors. On the other hand, cylindric tube bioreactor (CT) without bubble column resulted in minimum root growth (38.55 g dry weight) and kLa value (5.25 h$^{-1}$ ). Furthermore, the root growth (50.30 g dry weight) in BU with 10 cm bubble column more increased than 5 cm bubble column. However, the kLa value do not affected the secondary metabolite such as ginsenosides. These results show that the bubble column in air lift bioreactor increase kLa value and increased kLa value stimulate the growth of ginseng adventitious roots.

Cultural conditions and growth characteristics of indigo (Polygonum tinctorium) cells in an air-lift bioreactor (공기부양 생물반응기에서의 쪽 (Polygonum tinctorium) 세포배양의 생육조건 및 생육특성)

  • 신중한;이형주
    • KSBB Journal
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    • v.8 no.3
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    • pp.193-199
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    • 1993
  • To find out the optimum conditions for indigo cell culture in air-lift bioreactor, effects of media composition including nutrients and precursors of the indigo colorants on the cell growth and characteristics of the cell growth under various cultural conditions were analyzed. Optimum cultural conditions were tested and the growth characteristics were analyzed in external and internal loop type air-lift bioreactors during 14-day culture. Better cell growth was obtained when the inoculum size was higher in the range of 0.5∼2.5% packed cell volume tested. In the sucrose concentration of 2 to 4%, the cell growth was better when the sucrose concentration was 4% (w/w) in both types of reactors. Sucrose was used up in the early stage of exponential phase of growth At the optimum concentration of a Precursor tryptophan at 1 U UW was 3.8 g/l in internal loop bioreactor, and 3.5 g/l in external one after 14 days of cultivation. Addition of indole showed negative effect on cell growth of suspension culture in air-lift biorector culture and cell mass of 2.5 g/l and 2.2 g/l were obtained in external and internal loop bioreactor, respectively. Selected inorganic nitrogen source potassium nitrate showed about 110% increase in cell growth than that of control. DCW was 16.34 g/l under optimum conditions during 14-day cultivation in internal loop bioreactor.

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Lincomycin Production in the culture of Streptomyces lincolnensis using crude soybean oil in air lift bioreactor

  • Cho, Ki-An;Cho, Hoon
    • Journal of environmental and Sanitary engineering
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    • v.23 no.2
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    • pp.27-33
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    • 2008
  • Using crude soybean oil as the sole carbon source, the lincomycin production from Streptomyces lincolnensis LC 345 was investigated in the air lift bioreactor. When 30 g/L of crude soybean oil was used, the maximum lincomycin concentration reached 0.89 g/L, after 5 days of culture. When CSL concentration was increased from 10 to 30 g/L, Lincomycin concentration was increased from 0.6 to 1.2. On the other hand, when CSL concentration was increased from 40 to 60 g/L, it was decreased from 1.15 to 0.7 g/L. Using these results, fed batch cultures for comparing the use of crude soybean oil and glucose as a conventional carbon source were carried out in a 5 L air lift bioreactor. When crude soybean oil was used as the sole carbon source, the maximum lincomycin concentration was 2.0 g/L, which was about 2.0 fold higher than that of glucose medium after 7 day of culture. The product yield from olive oil was 0.042 g/g consumed carbon source, which was about 3.8 fold higher than that of glucose.

Optimization of Propagation of Anagrapha falcifera Nuclear Polyhedrosis Virus in Spodoptera Frugiperda 21 Cells

  • Lee, Jong-Min;Chang, Kyung-Hwa;Park, Jin-O;Park, Jong-Hwa;Hwang, In-Sook;Lee, Youn-Hyung;Yang, Jai-Myung;Chung, In-Sik
    • Journal of Microbiology and Biotechnology
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    • v.10 no.5
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    • pp.728-732
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    • 2000
  • Propagation of Anagrapha falcifera nuclear polyhedrosis virus(AfNPV) was investigated using well-plates and split-flow air-lift bioreactors. In well-plate experiments, the effects of pH, cell density at a point of infection, serum concentration, DEAE-dextran, and lipid on virus propagation were all closely examined. The AfNPV titer in well-plates was optimal at pH 6.8 and $3{\times}10^6$ cells/$cm^2$. The virus titer was not dramatically affected when the fetal bovine serum concentration was reduced from 10% to 5%. The addition of cholesterol at AfNPV infection of Sf21 cells enhanced the virus titer, whereas the addition of DEAE-dextran did not improve the titer. The AfNPV titer ($3.8{\times}10^7$ $TCID_{50}/ml$) at optimized conditions for well-plate experiments was 2.5-fold higher than for the control. In bioreactor experiments, the AfNPV titer showed its maximum level at air flow rates of 20-40 ml/min. In a split-flow air-lift bioreactor, AfNPV titer ($2.3{\times}10^7\;TCID_{50}/ml$) was 1.5-fold higher than the control when the culture was at pH 6.8 and supplemented with 0.34 mM cholesterol.

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Micropropagation of Lilium longiflorum 'Geogia' by Using Bioreactor. (생물반응기를 이용한 Lilium longiflorum ′Geogil′의 대량번식)

  • Han, Bong-Hee;Suh, Eun-Jung;Yae, Byeoung-Woo;Yu, Hee-Ju
    • Journal of Plant Biotechnology
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    • v.31 no.3
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    • pp.197-201
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    • 2004
  • Shoot clusters were induced from bulb scales of Lilium longiflorum 'Geogia', and proliferated on medium containing 0.5 mg/L BA and 0.5 mg/L IAA. Thereafter, these shoot clusters were cultured in 5 L air-lift bioreactors to form and grow normal bulblets. Number of bulblets increased on medium with 30 g/L sucrose, but growth of bulblets was effective on medium with 60 g/L sucrose. The number of bulblets from shoot clusters had no differences, though bulblet growth was very effective on medium with between full and double strength of MS salts. The inoculation of 100 g shoot clusters as a cultural material was suitable for formation and growth of bulblets in 5 L bioreactors. Air-lift type was more effective for the formation and growth of bulblets than that in ebb and flood one, and 200∼300 mL$.$min$^{-1}$ injection of air was suitable in growth of bulblets.

Improvement of Organics and Nitrogen Removal by HRT and Recycling Rate in Air Lift Reactors (공기부상반응조에서 체류시간과 반송율에 의한 유기물질 및 질소제거 향상에 관한 연구)

  • Kim, Jin-Ki;Yu, Sung-Whan;Lim, Bong-Su
    • Journal of Korean Society on Water Environment
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    • v.22 no.1
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    • pp.45-50
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    • 2006
  • This study was performed to evaluate the air lift reactors (ALR) by variations of HRT and recycling rate. Air lift reactor was composed of bioreactor and clarifier above it. To remove organic matters and nitrogen through the formation of microbic film and filtration, bio-filter reactors were filled with clay, glass, bead, waste plastic, respectively. Influent wastewater was fed to biofilter reactor, and effluent wastewater from bio-filter reactor was injected ALR again, instead of adding external carbon source. Effluent BOD concentration was satisfied with lower than 10 mg/L in recycling rate 100% regardless of the variation of HRT and the kinds of media materials. In HRT 4 hr, recycling rate 100%, BOD removal efficiency rate was from about 85 to 90%, COD removal efficiency rate was higher than 90%. Effluent TN concentration was satisfied with less than 20 mg/L, if HRT was maintained by over than 6 hr regardless of recycling rate and media materials. Over than HRT was 4 hr, microbes concentration in air lift reactor was maintained over than 2,500 mg/L constantly, not sensitive to environmental condition, and organic removal was effective as it was higher.

Biochemical Analysis of Baculovirus-insect Cell Interaction: I. Improved Recombinant ${\beta}-Galactosidase$ Production Using Medium Additives at AcNPV Infection of Insect Cells (Baculovirus-곤충세포 상호반응에 대한 생화학적 연구 -I. AcNPV의 곤충세포 감염시 배지 첨가물을 이용한 재조합 ${\beta}-galactosidase$ 생산 향상-)

  • Lee, Ki-Woong;Kim, Tae-Yong;Chung, In-Sik
    • Applied Biological Chemistry
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    • v.38 no.6
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    • pp.485-489
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    • 1995
  • The medium additives such as fatty acid, lipid, mannose, folic acid, $CaCl_2$ were examined to enhance recombinant ${\beta}-galactosidase\;({\beta}-gal)$ production in T-flask and air-lift bioreactor. The addition of each component. such as cholesterol, tocopherol, tricaprylin, mannose and folic acid at AcNPV infection of Tn5B1-4 cells enhanced ${\beta}-gal$ production, whereas the addition of $CaCl_2$ did not increase ${\beta}-gal$ production. The recombinant ${\beta}-gal$ production using the infection medium supplemented with a mixture of 0.34 mM cholesterol, 2.2 mM mannose and 0.045 M folic acid was enhanced 2 fold in an air-lift bioreactor, compared to the basal medium.

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