• Title, Summary, Keyword: A30

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miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs

  • Park, Deokbum;Kim, Hyuna;Kim, Youngmi;Jeoung, Dooil
    • Molecules and Cells
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    • v.39 no.4
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    • pp.299-309
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    • 2016
  • We have previously reported the role of miR-217 in anti-cancer drug-resistance. miRNA array and miRNA hybridization analysis predicted miR-30a-3p as a target of miR-217. miR-30a-3p and miR-217 formed a negative feedback loop and regulated the expression of each other. Ago1 immunoprecipitation and co-localization analysis revealed a possible interaction between miR-30a-3p and miR-217. miR-30a-3p conferred resistance to anti-cancer drugs and enhanced the invasion, migration, angiogenic, tumorigenic, and metastatic potential of cancer cells in CAGE-dependent manner. CAGE increased the expression of miR-30a-3p by binding to the promoter sequences of miR-30a-3p, suggesting a positive feedback loop between CAGE and miR-30a-3p. miR-30a-3p decreased the expression of p53, which showed the binding to the promoter sequences of miR-30a-3p and CAGE in anti-cancer drug-sensitive cancer cells. Luciferase activity assays showed that p53 serves as a target of miR-30a. Thus, the miR-30a-3p-CAGE-p53 feedback loop serves as a target for overcoming resistance to anti-cancer drugs.

Comparison of quality of 30:2 vs. 2:30 CPR in manikins (심폐소생술 방법 변화에 따른 quality 비교 - 30:2와 2:30 비교분석실험 -)

  • Uhm, Tai-Hwan;Yoou, Soon-Kyu;Choi, Hea-Kyung;Jung, Ji-Yeon
    • The Korean Journal of Emergency Medical Services
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    • v.14 no.3
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    • pp.71-81
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    • 2010
  • Purpose: To minimize an interruption in chest compression, reduce the hands-off time, the American Heart Association has recommended the ratio of chest compression to ventilation ratio to 30:2 from 2005 CPR guideline to 2010 CPR guideline. However, current studies have shown that the hands-off time was > 10 seconds with that method. For this reason, we devised new CPR method that a ventilation to chest compression ratio of 2:30 to reduce pt assessment time and skipped the assessment step of carotid artery pulse would be a more effective way to reduce the hands-off time & the time to set the CPR. According to the more detailed purpose are listed below. 1) We would like to confirm efficiency of a ventilation to chest compression ratio of 2:30 than a chest compression to ventilation ratio of 30:2 to reduce the hands-off time & the time to set the CPR. 2) We would like to evaluate possibility of increasing for chest compression accuracy of a ventilation to chest compression ratio of 2:30 than a chest compression to ventilation ratio of 30:2 3) We would like to evaluate possibility of increasing for ventilation accuracy of a ventilation to chest compression ratio of 2:30 than a chest compression to ventilation ratio of 30:2 Methods: According to 2005 American Heart Association Guidelines, 60 paramedic students(20 students X freshmen, sophomore, junior) performed 5 cycles of 3~ chest compressions : 2 ventilations after A, B, C evaluation with Laerdal Resusci R Anne SkillReporters. After 5 minutes rest, the 60 students performed 5 cycles of 2 ventilations : 30 chest compressions after A, B evaluation with the manikins between 13 and 17 September 2010. The short reports including speed & accuracy of chest compression, respiratory, CPR cycle were gained from the manikins. Hands-off times were measured by assistants. Results: Recently, the importance of high quality CPR was emphasized in order to perform the CPR faster and more accurate. To find out improving the conventional CPR method, we switch the procedure of the compression and the ventilation. By switching the procedure back and forth, we are able to compare the effectiveness of CPR between two type of CPR method which are 2:30 and 30:2 methods. 2:30 is that the breaths is delivered twice, first and perform 30 compressions while 30:2 perform 30 compressions first and give 2 breaths followed by the ABC method. Also, we verify the effectiveness of the hands off time, compression accuracy of the compression through the comparison of the two procedure as mentioned earlier. Consequently research verified that 2:30 is the efficient by providing faster set up delivering more accurate chest compression. Conclusion: 2:30 can minimize a time delay from cardiac standstill until starting the chest compression. In addition, hands-off time which is an interruption in chest compression can be shortened by 2:30 method, which result to effective oxygenation of coronary artery & maintenance of the bloodstream. Once again, performing the 2:30 method provide lessen hands off time and increase the accuracy of the chest compression.

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Treatment with ultra-dilutions of Arnica montana increases COX-2 expression and PGE2 secretion in mouse chondrocytes (생쥐 연골세포에 Arnica montana 처리에 따른 COX-2 발현과 PGE2 분비 비교)

  • Kim, Yun Kyu;Yeo, Myeong Gu
    • Journal of the Korea Convergence Society
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    • v.10 no.2
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    • pp.331-337
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    • 2019
  • Objective: We studied the effects of 4x, 30x, 30c, and 200c homeopathic dilutions of A. montana on inflammation in primary cultured mouse chondrocytes. Methods: Examined expression of Coll-2 and COX-2, and secretion of PGE2. Results: Treatment with 4x, 30x, and 30c A. montana decreased mRNA expression of Coll-2 and 30x A. montana increased mRNA expression of COX-2, while treatment with 30x and 30c A. montana increased protein expression of COX-2. Treatment with the 30c A. montana increased release of PGE2. Conclusion: Treatment with A. montana induces dedifferentiation and inflammatory responses, including increased COX-2 expression and PGE2 secretion.

Comparative Analysis of Digital Elevation Models between AW3D30, SRTM30 and Airborne LiDAR: A Case of Chuncheon, South Korea

  • Acharya, Tri Dev;Yang, In Tae;Lee, Dong Ha
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.36 no.1
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    • pp.17-24
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    • 2018
  • DEM (Digital Elevation Model) is a useful dataset which represents the earth surface. Beside many applications, production and frequent update of DEM is a costly task. Recently global satellite based DEMs are available which has huge potential for application. To check the accuracy, this study compares two global DEMs: AW3D30 (Advanced Land Observing Satellite World 3D 30m) and SRTM30 (Shuttle Radar Topography Mission Global 30m) with reference resampled LiDAR DEM 30m in a test area around Chuncheon, Korea. The comparison analysis was based on statistics of each DEM, their difference, profiles, slope, basin and stream orders. As a result, it is found that SRTM30 and AW3D30 were much similar but inconsistent in the test area compared to the LiDAR30 DEM. In addition, SRTM30 shows less difference with LiDAR30 compared to the AW3D30 DEM. But, DEMs should be very carefully examined for area which has temporal or season changes. For basin and stream analysis, global DEMs can be used only for regional scale analysis not local large scales.

Determination of Mean Shear Wave Velocity to the Depth of 30m Based on Shallow Shear Wave Velocity Profile (얕은 심도 전단파속도 분포를 이용한 30m 심도 평균 전단파속도의 결정)

  • Sun, Chang-Guk;Chung, Choong-Ki;Kim, Dong-Soo
    • Journal of the Earthquake Engineering Society of Korea
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    • v.11 no.1
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    • pp.45-57
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    • 2007
  • The mean shear wave velocity to the depth of 30 m (Vs30) derived from the western Vs is the current site classification criterion for determining the design seismic ground motion taking into account the site amplification potential. In order to evaluate the Vs30 at a site, a shear wave velocity (Vs) Profile extending to at least 30 m in depth must be acquired from in-situ seismic test. In many cases, however, the resultant depth of the Vs profile may not extend to 30 m, owing to the unfavorable field condition and the limitation of adopted testing techniques. In this study, the Vs30 and the mean shear wave velocity to a depth shallower, than 30 m (VsDs) were computed from the Vs profiles more than 30 m in depth obtained by performing various seismic tests at total 72 sites in Korea, and a correlation between Vs30 and VsDs was drawn based on the computed mean Vs data. In addition, a method for extrapolating the Vs profile from shallow depth to 30 m was developed by building a shape curve based on the average data of all Vs profiles. For evaluating the Vs30 from the shallow Vs profiles, both the methods using VsDs and shape curve result in less bias than the simplest method of extending the lowermost Vs equally to 30 m in depth, and are usefully applicable particularly in the cases of the Vs profiles extending to at least 10 m in depth.

Expression of CD30 in Testis and Epididymis of Adult Mice

  • Choo, Young-Kug;Nam, Sang-Yun
    • Animal cells and systems
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    • v.8 no.3
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    • pp.197-203
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    • 2004
  • CD30 is a member of tumor necrosis factor receptor (TNFR) superfamily and has pleiotropic functions including cell activation, proliferation, differentiation, and death, depending on cell types and stage of differentiation. Although CD30 expression has been described mainly in hematopoietic tissues, several types of nonhematopoietic tumors including embryonic carcinoma and germ-cell tumors express CD30. We examined CD30 distribution in the testis and epididymis from wild type and CD30-deficient mice. In the testis, spermatogonia, spermatocytes and Sertoli cells expressed CD30, but not in spermatids. Spermatogonia and spermatocytes near the basement membrane strongly reacted to anti-CD30. In the epididymis, CD30 expression was exclusively observed in luminal epithelia and some interstitial cells. Taken together, these results show a spatio-temporal regulation of CD30 expression in mouse testis and epididymis and suggest a possible role of CD30 in spermatogonia and spermatocytes.

Ultrafiltration and Separation Process Optimization of Hen Egg White Lysozyme as Natural Antimicrobial Enzyme (천연 항균 효소제 난백 lysozyme의 한외여과 조건 최적화)

  • Lee, Eun-Young;Woo, Gun-Jo
    • Korean Journal of Food Science and Technology
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    • v.30 no.2
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    • pp.397-406
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    • 1998
  • Hen egg white lysozyme (HEWL) is very valuable as a natural preservative in food processing due to its selective bactericidal activity. HEWL which traditionally isolated by crystallization or freeze drying was simply separated from 13 different hen egg white (HEW) proteins by a single-step ultrafiltration. Freeze dried HEW (0.25%, w/v) dissolved in a citrate-phosphate buffer (pH 4.6) was ultrafiltered with a PM30 membrane under various operating conditions, by changing concentration, temperature, transmembrane pressure $({\triangle}P_T)$, and stirring speed. Optimum separation conditions were decided when maximal flux was obtained. Under the optimum separation conditions, the effect of membrane material and fouling on flux as time passed as well as lysozyme concentration, protein concentration, specific activity (SA) in the permeate were measured. Best separation conditions of HEWL with PM30 membrane were sample concentration 0.25%, temperature $35^{\circ}C$, ${\Delta}P_T\;30\;psi$, and stirring speed 300 rpm. During the first 12 min, the flux of YM30 was higher, but at the steady-state it was lower than that of PM30. The SA of the PM30 permeate was over 2 times higher in spite of the lysozyme and protein concentration being lower than that of YM30 permeate. The flux of 5 times used PM30 decreased 30% compared to a new PM30, but both had the same tendency in flux decrease when time passed. Both of them reached a steady-state after 35 min and remained at 70% of the initial flux. In the PM30 permeate, the lysozyme concentration and SA were 110 units/mL and 2,821 units/mg protein, respectively. Therefore, PM30 membrane separation was very effective for separation of antimicrobial lysozyme.

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Soluble CD30: A Possible Serum Tumor Marker for Primary Effusion Lymphoma

  • Michai, Manthana;Goto, Hiroki;Hattori, Shinichiro;Vaeteewoottacharn, Kulthida;Wongkham, Chaisiri;Wongkham, Sopit;Okada, Seiji
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.10
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    • pp.4939-4941
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    • 2012
  • Background: The serum level of soluble CD30 (sCD30) is known to be increased with several lymphomas and to correlate with prognosis. Primary effusion lymphoma (PEL) is a highly aggressive malignant lymphoma with poor prognosis, but the existence and significance of sCD30 in PEL have not yet been investigated in detail. Objectives: Since the membrane type of CD30 is frequently expressed on the surface of PEL cells, we compared the expression of the membrane type of CD30 and the production of sCD30 among PEL cell lines as well as other lymphomas. Methods: The expression of surface CD30 in various lymphoma cell lines was analyzed with flow cytometry ans sCD30 was quantified by ELISA. Results: Both surface and sCD30 were detected on PEL cell lines as well as on Hodgkin's lymphoma and adult T-cell leukemia/lymphoma cell lines. Surface CD30 and sCD30 levels of each cell lines correlated with each other. Conclusion: The serum level of sCD30 appear to be a useful biological tumor marker for the diagnosis and management of CD30-positive PEL.

Immunization effect of recombinant P27/30 protein expressed in Escherichia coli against the hard tick Haemaphysalis longicornis (Acari: Ixodidae) in rabbits

  • You, Myung-Jo
    • The Korean Journal of Parasitology
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    • v.42 no.4
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    • pp.195-200
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    • 2004
  • We investigated the induction of resistance to Haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant H. longicornis P27/30 protein. The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of H. longicornis, and identified P27/30 as a troponin I-like protein. In this study, rabbits that were immunized with recombinant P27/30 expressed in Escherichia coli showed the statistically significant longer feeding duration for larval and adult ticks (P<0.05), low engorgement rates in larval ticks (64.4%), and an apparent reduction in egg weights, which suggest that H. longicornis P27/30 protein is a potential candidate antigen for a tick vaccine. These results demonstrated that the recombinant P27/30 protein might be a useful vaccine candidate antigen for biological control of H. longicornis.

A Trend of the Bathroom Planning of 30s Pyung Apartments (30평형대 아파트 욕실 공간의 변화추이)

  • Hwang, Yun-Jung;Shin, Kyung-Joo
    • Proceedings of the Korean Institute of Interior Design Conference
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    • pp.48-51
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    • 2005
  • The purpose of this study is to provide a basic study for a desirable bathroom planning of the 30s pyung apartments. This study explores the change of the bathroom planning and analyzes the bathroom plans of the existing apartments. The data are 927 case of the bathroom plans of the 30s pyung apartments constructed in Seoul and the new town between 1971-2003. Where 145,807 households live. In this study, a trend of the bathroom form, numble in the 30s pyung apartments are examined. For the better planning of the bathroom. factors like family size. life cycle, and manner of bathroom use should be analyzed in the future studies. The analysis of the dweller's consciousness, satisfaction, and the demands on the bathroom in 30s pyung apartments, and to give useful information on bathroom planning of 30s pyung apartments.

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