• Title, Summary, Keyword: 2,2-diphenyl-1-picrylhydrazyl (DPPH)

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Alatemin, Cassiaside and Rubrofusarin gentiobioside, Radical Scavenging Priniciples from the Seeds of Cassia tora on 1,1-Diphenyl-2-picrylhydrazyl(DPPH) Radical

  • Choi, Jae-Sue;Lee, Hee-Jung;Kang, Sam-Sik
    • Archives of Pharmacal Research
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    • v.17 no.6
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    • pp.462-466
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    • 1994
  • Radical scavenging principles on 1, 1-diphenyl-2-picylhydrazyl(DPPH) radical were isolated from the seeds of Cassis tora L. Assignments of the^1H-{\;}and{\;}^{13}C-NMR$ data showed the active components to be an anthraquinone, alatemin nad two naphthopyrone glycosides, nor-rubro-fusarin-6-.betha.-D-glucoside(cassiaside) and rubrofusarin-6- -D-gentiobioside. Altemin showed more potent radical sacvenging effect than the others.

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The Scavenging Effect of 207 Oriental Medicines and 19 Oriental Prescriptions on 1,1-Diphenyl-2-Picrylhydrazyl Radical (207종 한약과 활성 한약이 포함된 19종 한약 방제의 1,1-Diphenyl-2-Picrylhydrazyl 라디칼 소거작용)

  • Kim Min-San;Her Jong-Moon;Park Jong-Cheol
    • Herbal Formula Science
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    • v.12 no.1
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    • pp.159-175
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    • 2004
  • The inhibitory effects of 207 oriental medicines and 19 oriental prescriptions on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were examined. The most effective oriental medicines were the fruit of Terminalia chebula, followed in order by the seed of Areca catechu, the heart wood of Caesalpinia sappan, the pericarp of Castanea crenata, the seed of Nelumbo nucifera, Rhus galls, the root of Sanguisorba officinalis, and the bark of Betula platyphylla at the concentration of 10 ${\mu}g$/mL. Of the 19 oriental prescriptions, Buyeonsan and Samsinhwan showed the potent scavenging activities of 88% and 71% on DPPH radical at the concentration of 5 ${\mu}g$/mL, respectively.

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Antioxidant Properties of Erigeron annuus Extract and Its Three Phenolic Constituents

  • Lee Hee-Jung;Seo Young-Wan
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.1
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    • pp.13-18
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    • 2006
  • The antioxidant activity of the extract of Erigeron annuus was assessed by means of two different in vitro tests: bleaching of the stable 1,1-diphenyl-2-picrylhydrazyl radical (DPPH test) and the scavenging of authentic peroxynitrite in company with peroxynitrite generation from 3-morpholinosydnonimine (SIN-1). In both tests, the 85% aq. MeOH and n-BuOH soluble fractions of the crude extract showed a significant scavenging effect on peroxynitrite and DPPH radical in comparison to L-ascorbic acid. And bioassay-guided fractionation of the n-BuOH soluble fraction led to the isolation of three compounds: Apigenin (1), quercetin-3-O-glucoside (2), and caffeic acid (3). The structures of the isolated compounds were elucidated on the basis of their spectroscopic data and their antioxidant activities were measured by determining their capacity to scavenge peroxynitrite and the DPPH radical.

Further Isolation of Peroxynitrite and 1, 1-Diphenyl-2-picrylhy-drazyl Radical Scavenging Isorhamnetin 7-Ο-glucoside from the Leaves of Brassica juncea L.

  • Choi, Jae-Sue;Jung, Mee-Jung;Park, Hye-Jin;Chung, Hae-Young;Kang, Sam-Sik
    • Archives of Pharmacal Research
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    • v.25 no.5
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    • pp.625-627
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    • 2002
  • From the leaves of Brassica juncea, an radical scavenging isorhamnetin 7-Ο-glucoside on peroxynitrite and 1,1-diphenyl-2-picrylhydrazyl (DPPH) was isolated and characterized based on the spectroscopic evidence. The compound showed the peroxynitrite and DPPH scavenging activities with $IC_{50}$ values of 2.07$\pm$0.17 and 13.3 $\mu$M, respectively. Penicillamine and L-ascorbic acid as positive control exhibited radical scavenging activities with $IC_{50}$ values of 3.17$\pm$0.39 and 12.78 $\mu$M, respectively.

Further Isolation of Antioxidative $(+)-1-Hydroxypinoresinol-1-O-{\beta}-D-glucoside$ from the Rhizome of Salvia miltiorrhiza that Acts on Peroxynitrite, Total ROS and 1,1-Diphenyl-2-picrylhydrazyl Radical

  • Kang, Hye-Sook;Chung, Hae-Young;Byun, Dae-Seok;Choi, Jae-Sue
    • Archives of Pharmacal Research
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    • v.26 no.1
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    • pp.24-27
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    • 2003
  • A furanofuranoid lignan glycoside, with radical scavenging on peroxynitrite, total reactive oxygen species (ROS) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, was isolated from the rhizome of Salvia miltiorrhiza and characterized as (+)-1-hydroxypinoresinol-1-Ο-$\beta$-D-glucoside based on spectroscopic evidence. The compound exhibited peroxynitrite, total ROS and DPPH radical scavenging activities with $IC_{50}$ values of 3.23$\pm$0.04, 2.26$\pm$0.07 and 32.3$\pm$0.13 $\mu$M, respectively. Penicillamine, Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) and L-ascorbic acid, acting as positive controls, showed radical scavenging activities with $IC_{50}$ values of 6.72$\pm$0.25, 1.43$\pm$0.04 and 11.4$\pm$0.07 $\mu$M, respectively.

Inhibition of 5α-reductase of de novo Generation of Short Anti-oxidant Peptides

  • Lee, Sung-Gyu;Kang, Hyun
    • Biomedical Science Letters
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    • v.24 no.3
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    • pp.263-269
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    • 2018
  • This study aims to investigate the biological activities related to hair loss of short anti-oxidant peptides (DK peptides) $5{\alpha}$-reductase inhibition and anti-oxidation. The series of DK peptides were generated amphipathic helical properties using leucines, lysines and tryptophan residues. Cell viability and free radical scavenging activities were performed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The DK peptides were investigated for inhibitory activity against $5{\alpha}$-reductase. Antioxidant activities were determined by means of, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays. All peptides could inhibit $5{\alpha}$-reductase in lipopolysaccharide-stimulated macrophage. In conclusion, DK peptides was suggested as the most attractive ingredients for improving hair loss, because of the high inhibitory against $5{\alpha}$-reductase inhibition.

Synthesis of Heterocyclic Chalcone Derivatives and Their Radical Scavenging Ability Toward 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Free Radicals

  • Hwang, Ki-Jun;Kim, Ho-Seok;Han, In-Cheol;Kim, Beom-Tae
    • Bulletin of the Korean Chemical Society
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    • v.33 no.8
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    • pp.2585-2591
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    • 2012
  • A series of heterocyclic chalcone derivatives bearing heterocycles such as thiophene or furan ring as an isostere of benzene ring were carefully prepared, and the influence of heterocycles on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities was systematically investigated. Structure-activity relationships (SAR) analysis showed that the activities of thiophene ring-containing chalcones were higher than those of furan ring-containing chalcones, and the presence of methyl substituent of heterocyclic ring distinctly affected the activities compared with non-substituted heterocycles in an opposite manner, with the 4'-methyl group of thiophene ring increasing activity and the 3'-methyl group of the furan ring decreasing activity. The distinct isosteric effect of heterocycles (i.e., thiophene or furan ring) on radical scavenging activities of heterocyclic chalones was distinctly demonstrated in our work.

A Continuous Spectrophotometric Assay for NADPH-cytochrome P450 Reductase Activity Using 1,1-Diphenyl-2-Picrylhydrazyl

  • Yim, Sung-Kun;Yun, Su-Jung;Yun, Chul-Ho
    • BMB Reports
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    • v.37 no.5
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    • pp.629-633
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    • 2004
  • NADPH-cytochrome P450 reductase (CPR) transfers electrons from NADPH to cytochrome P450, and catalyzes the one-electron reduction of many drugs and foreign compounds. Various forms of spectrophotometric titration have been performed to investigate the electron-accepting properties of CPR, particularly, to examine its ability to reduce cytochrome c and ferricyanide. In this study, the reduction of 1,1-diphenyl-2-picrylhydrazyl (DPPH) by CPR was assessed as a means of monitoring CPR activity. The principle advantage of DPPH is that its reduction can be assayed directly in the reaction medium by a continuous spectrophotometry. Thus, electrons released from NADPH by CPR were transferred to DPPH, and DPPH reduction was then followed spectrophotometrically by measuring $A_{520}$ reduction. Optimal assay concentrations of DPPH, CPR, potassium phosphate buffer, and NADPH were first established. DPPH reduction activity was found to depend upon the strength of the buffer used, which was optimal at 100 mM potassium phosphate and pH 7.6. The extinction coefficient of DPPH was $4.09\;mM^{-1}\;cm^{-1}$. DPPH reduction followed classical Michaelis-Menten kinetics ($K_m\;=\;28\;{\mu}M$, $K_{cat}\;=\;1690\;min^{-1}$). This method uses readily available materials, and has the additional advantages of being rapid and inexpensive.

(+)-Catechin, an Antioxidant Principle from the Leaves of Pinus densiflora that Acts on 1,1-Diphenyl-2-picrylhydrazyl Radical

  • Choi, Jin-Ho;Kim, Hyun-Sook;Jung, Mee-Jung;Choi, Jae-Sue
    • Natural Product Sciences
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    • v.7 no.1
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    • pp.1-4
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    • 2001
  • The antioxidant activity of Pinus densiflora was determined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The methanol extract of P. densiflora showed strong antioxidant activity, and thus fractionated with several solvents. The antioxidant activity potential of the individual fraction was in the order of ethyl acetate>n-butanol>water>dichloromethane> n-hexane fraction. The ethyl acetate soluble fraction exhibiting strong antioxidant activity was further purified by repeated silica gel column chromatography. Antioxidant (+)-catechin was isolated as one of the active principles from the ethyl acetate fraction, together with the inactive components, dihydrokaempferol and 1-O-benzoylglucose. The radical scavenging effect of (+)-catechin on DPPH radical exceeded to that of L-ascorbic acid which is a well known antioxidant.

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Antioxidant Constituents from Setaria viridis

  • Kwon, Yong-Soo;Kim, Eun-Young;Kim, Won-Jun;Kim, Woo-Kyung;Kim, Chang-Min
    • Archives of Pharmacal Research
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    • v.25 no.3
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    • pp.300-305
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    • 2002
  • The EtOAc and n-BuOH soluble fractions from the aerial part of Setaria viridis showed a strong free radical scavenging activity. Six major compounds were isolated from these fractions. They were identified by spectral data as tricin (1), p-hydroxycinnamic acid (2), vitexin 2"-Ο-xyloside (3), orientin 2"-Ο-xyloside (4), $tricin-7-Ο-{\beta}-D-glucoside$ (5) and vitexin 2"-Ο-glucoside (6). Among these compounds, 4 and 5 exhibited strong free radical scavenging activities on 1, 1-diphenyl-2-picrylhydrazyl (DPPH). We further studied the effects of these isolated compounds on the lipid peroxidation in rat liver microsomes induced by non-enzymatic method. As expected, 4 and 5 exhibited significant inhibition on $ascorbic/Fe^{2-}$ induced lipid peroxidation in rat liver microsomes.ver microsomes.