• Title, Summary, Keyword: 181c (miR-181c)

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Upregulation of MicroRNA 181c Expression in Gastric Cancer Tissues and Plasma

  • Cui, Mei-Hua;Hou, Xiao-Lin;Lei, Xiao-Yan;Mu, Fang-Hong;Yang, Gui-Bin;Yue, Lin;Fu, Yi;Yi, Guo-Xing
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.5
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    • pp.3063-3066
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    • 2013
  • Objective: To test the microRNA-181c (miR-181c) expression in tissues and plasma of gastric cancer (GC) cases, analyze any correlations, and explore the possibility of miR-181c as a potential molecular marker for GC diagnosis. Materials and Methods: Relative miR-181c expression levels in cancers and plasma from 30 GC patients was tested using reverse transcription-real-time fluorescent quantitation PCR and compared to that in samples from 30 gastric ulcer and 30 chronic gastritis patients. Results: The miR-181c expression level in the GC tissues was significantly higher than that in the gastric ulcer and chronic gastritis tissues (P = 0.000), as was the miR-181c expression level in the GC plasma (P = 0.000). We determined that miR-181c expression in GC plasma was positively correlated to its expression in the GC tissues (P = 0.000). Conclusions: The expression of miR-181c is upregulated in GC tissues and plasma, and the miR-181c expression level in GC plasma is positively correlated to that in the corresponding cancer tissues. Plasma miR-181c is possibly a new serological marker for GC diagnosis.

MicroRNA Expression in Leukemia Cell Line(K562 cell) Using Rhus Verniciflua Stokes (건칠(乾漆)을 이용한 K562 만성 골수성 백혈병 세포주에서의 MicroRNA 발현 규명)

  • Choi, Hyun Sook
    • The Korea Journal of Herbology
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    • v.34 no.6
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    • pp.71-78
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    • 2019
  • Objective : The purpose of the study was to identify expression profiling of miRNAs associated with cancers after treating allergen-removed Rhus Verniciflua Stokes and allergen-removed Rhus Verniciflua Stokes fumigaed Angelica gigas on leukemia cell lines. Methods : miRNA expression has been analyzed using miRNA array method through denaturation and hybridization after isolating the total RNA from leukemic cell line treated with 100 ㎍/㎖ of aRVS and aRVS-A each. Microarray expressions were interpreted as 'significant' on miRNAs when decreased less than 0.5 fold or increased more than 1.5 fold compared with the control group. Results : Among 158 miRNAs in total, 32 miRNAs were significantly presented in miRNAs expression. miRNA has been activated with a variety of genes for predicted targets, and the overexpressed miRNAs were categorized according to proliferation and metastasis of cancer in this study. The findings were reported that seven miRNAs (let-7b, miR-193a-5p, 296-3p, 26a, 22, 124a, 92b) showed significant expressions on proliferation and growth, seven miRNAs (miR-193a-5p, 26a, 200c, 183, 124a, 198, 210) presented meaningful expressions on invasion and metastasis, two miRNAs (let-7b, miR-210) were highly expressed on angiogenesis, five miRNAs (let-7b, miR-26a, 181d, 181c, 296-5p) related with apoptosis, and six miRNAs (let-7b, miR-200c, 183, 370, 124a, 191) were associated with prognosis of cancer and early diagnostic factors for cancer. Conclusion : The mechanism of miRNA takes a role in diagnosis, treatment, and prognotic factors for cancer as well. This study suggested that further detailed research on overexpression of specific miRNA should be carried out continuously in the future.

The Relationship between Emotional Intelligence, Critical Thinking Disposition and Clinical Competence in New Graduate Nurses immediately after Graduation (졸업직후 신규간호사의 감성지능, 비판적 사고능력 및 임상수행능력 간의 관계)

  • Lee, Oi Sun;Kim, Mi Jeoung
    • Journal of Digital Convergence
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    • v.16 no.6
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    • pp.307-315
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    • 2018
  • The purpose of this study is to examine to identify the mediating effects of emotional intelligence in the relation between critical thinking disposition and clinical competence among new graduate nurses immediately after graduation. Subjects were 181 new graduate nurses immediately after graduation. in two general hospitals in C city and K city. Data were collected by self-report questionnaires from February 27 to March 30, 2017. Data were analyzed by descriptive statistics, Independent t-test, ANOVA, Pearson's correlation and Multiple regression and Sobel test to test the significance of a mediation effect using SPSS 23 software. Emotional intelligence were significantly positive correlations with critical thinking disposition(r=.62, p<.001) and clinical competence(r=.60, p<.001) in new graduate nurses immediately after graduation. Emotional intelligence partially mediated the relationship between critical thinking disposition and clinical competence which showed significance in Sobel's test(Z=3.88, p<.001). Theses results indicated that in order to improve the clinical competence of new nurse, it is necessary to develop and test the program for improving not only on the critical thinking disposition but also on the emotional intelligence.

Three Crystal Structures of Dehydrated Partially $Co^{2+}-Exchanged$ Zeolite A Treated with Potassium Vapor (부분적으로 코발트 이온으로 치환한 제올라이트 A를 진공 탈수한 후 칼륨 증기로 반응시킨 3개의 결정구조)

  • Jeong Mi Suk;Jang Se Bok
    • Korean Journal of Crystallography
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    • v.15 no.2
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    • pp.59-68
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    • 2004
  • Three crystal structures of dehydrated partially $Co^{2+}-exchanged$ zeolite A treated with 0.6 Torr of K at $300^{\circ}C$ (for 12 hrs, 6 hrs, and 2 hrs) vapor have been determined by single-crystal X-ray diffraction techniques in the cubic space group Pm3m at 21(1)$^{\circ}C(a=12.181(1)\;{\AA},\;a=12.184(1)\;{\AA},\;and\;a=12.215(1)\;{\AA})\;respectively)$. Their structures were refined to the final error indices, R(weight) of 0.090 with 10 reflections, 0.091 with 82 reflections, and 0.090 with 80 reflections, respectively, for which $1>\sigma(I)$. In each structure, all four $Co^{2+}$ and four $Na^+$ ions to be reduced by K atoms. The cobalt and sodium atoms produced are no longer found in the zeolite. K species are found at five different crystallographic sites: three $K^+$ ions lie at the planes of 8-rings, filling that position, ca. 11.5 K^+$ ions lie on threefold axes, ca. 4.0 in the large cavity and ca. 4.0 in the sodalite cavity, and ca. 0.5 $K^+$ ion is found near a 4-ring. ca. three $K^0$ atoms are found deep into the large cavity on threefold axes. In these structures, crystallographic results show that cationic tetrahedral $K_4$ (and/or triangular $K_3$) clusters have formed in the sodalites of zeolite A. The $K_4$ and/or $K_3$ clusters coordinate trigonally to three oxygens of a six-oxygen ring. The partially reduced ions of these clusters interact primarily with oxygen atoms of the zeolite structure rather than with each other. ca. 14.5K species are found per unit cell, more than the twelve $K^+$ ions needed to balance the anionic charge of zeolite framework, indicating that sorption of $K^0$ has occurred. The three $K^0$ atoms in the large cavity are closely associated with three out of four $K^+$ ions in the large cavity to form $K_7^{4+}$ clusters. The $K_7^{4+}$ cluster not interacts primarily with framework oxygens.

A Study for measurement method of P-wave duration in Paroxysmal Atrial Fibrillation(PAF) subjects (발작성 심방세동 환자의 P파 간격 측정 방법에 관한 연구)

  • Lee, J.Y.;Yeo, H.S.;Han, W.T.;Kim, I.Y.;Lee, B.C.;Kim, J.S.;Mi, J.S.;Seo, J.D.;Lee, W.R.
    • Proceedings of the KOSOMBE Conference
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    • v.1998 no.11
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    • pp.181-182
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    • 1998
  • In previous study for correlation between P-wave Signal Averaged Electrocardiography (SAECG) and Paroxysmal Atrial Fibrillation (PAF) subjects, we showed that the duration of P-wave in subjects is longer than in controls. In this respect, the P-wave SAECG is a new method proving to be an accurate and independent noninvasive marker for the risk of PAF. To prove this suggestion, accurate detection and alignment of P-wave are indispensible. In previous study, we measured P-wave duration by manual. So it was not accurate and consistent. To measure the P-wave duration accurately and automatically, we have developed an automatic algorithm for P-wave duration measurement. We showed that the duration of P- wave in the subjects is longer than in controls with this algorithm.

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Evaluation of marginal fidelity of copy-milled and CAD/CAM all ceramic crowns

  • Jeong Seung-Mi;Kang Dong-Wan;Wolf Christoph
    • The Journal of Korean Academy of Prosthodontics
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    • v.39 no.3
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    • pp.243-249
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    • 2001
  • Statement of the problem. The interest in all-ceramic restorations has increased as more techniques have become available. With the introduction of machinable dental ceramics and CAD/CAM systems or Copy-milling systems there is a need for evaluating the quality levels of these new fabrication techniques. Purpose. This study was to evaluate the fitting accuracy of machined all-ceramic crowns made out of an industrially prefabricated feldspathic porcelain. Material and Methods. Three master models with different cutting depth (0.8mm/1.0mm/1.2mm)were produced using a palladium-silver alloy. A total of 36 working dies, 12 of each form, was used for the modellation of prototype resin copings and 36 additional crowns, 12 of each cutting depth, were produced by using the $CEREC^{(R)}2$ system for all crowns. The maginal fit of all 72 crowns was then evaluated on their respective master die at 54 circularly staggered points of measurement per crown under a fixation pressure of 30 N by using a computerized video image system. Results. The medians of the copy-milled $CELAY^{(R)}$ crowns ranged from 29 to $36{\mu}m$. The highest value for the marginal gap was found in group B (cutting depth 1.0mm) at $107{\mu}m$. The median for the $CEREC^{(R)}2$ crowns was found between 43.5 and $70{\mu}m$. The maximum values for all three groups ranged from $181{\mu}m$ to $286{\mu}m$. With $286{\mu}m$ the highest value for marginal gap was found in group C. the Kruskal-Wallis test and multiple comparisons analysis procedure revealed a significant influence of the production technique on the marginal fit in all three groups (p<0,02). Conclusion. 1. The $CELAY^{(R)}$ system is capable to produce all-ceramic crowns with a significantly better marginal fit than the $CEREC^{(R)}2$ system. 2. As far as premolar crowns produced with the $CEREC^{(R)}2$ system are concerned, the cutting depth has a significant influence on fitting accuracy. 3. The production of crowns with an acceptable marginal fit is possible with both systems. However, adhesive luting is recommended for milled feldspathic porcelain crowns.

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Effect of Nitrogen Fertilization levels on Growth and Isoflavone Content in Soybean (질소시비수준이 콩의 생육 및 isoflavone 함량에 미치는 영향)

  • Lee Mi-Ja;Park Jong-Chul;Oh Young-Jin;Kim Kyong-Ho;Kim Hyung-Soon;Lee Sang-Bok;Kim Jung-Gon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.5
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    • pp.445-450
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    • 2006
  • The nitrogen fertilization effect on growth characteristics and isoflavone content was investigated in this study, and isoflavone analyzed by HPLC with photodiode array (PDA) detector and reverse-phase $C_{18}$ column. Fertilization levels were no-fertilization, no nitrogen, 50% decreased in nitrogen, standard and 50% increased in nitrogen fertilization. The 50% increased nitrogen fertilization showed the highest growth characteristics then other fertilization level and the number of pod and seed showed maximum value 31.9 and 72.3, respectively, and seed yield was 2,460 kg/ha. During growth stages, isoflavone content in leaf, stem and root of soybean plants decreased to R5 stage then increased to R7 stage. Isoflavone content according to various nitrogen fertilization condition, in case of none fertilization, no nitrogen, 50% decreased nitrogen fertilization showed higher value than those of standard and 50% increased nitrogen fertilization levels. Aglycon content among the isoflavone isomers showed much higher in plant than in seed. The highest isoflavone content was found in the root of soybean plant parts. Isoflavone content of seed was higher in none, no nitrogen, 50% decreased nitrogen than those of standard and 50% increased nitrogen fertilization.