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A report of 11 unrecorded bacterial species in Korea isolated in 2017

  • Maeng, Soohyun;Kim, Ju-Young;Jang, Jun Hwee;Kang, Myung-Suk;Kim, Myung Kyum
    • Journal of Species Research
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    • v.7 no.2
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    • pp.135-150
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    • 2018
  • Eleven bacterial strains 17SD2_15, 17Sr1_23, 17SD2_13, 17Sr1_31, 17gy_18, 16B15D, 16B02D, 16B04G, 16B01D, 17U4-2 and 17J28-10 assigned to the phylum Proteobacteria were isolated from soil samples collected from Seoul Women's University, in South Korea. The Belnapia species, strain 17SD2_15 was cocci-shaped and pink-colored. The Methylobacterium species, strain 17Sr1_23, 17SD2_13, 17Sr1_31, and 16B15D were short rod-shaped and pink-colored. The Microvirga species, strain 17gy_18, and 16B02D were short rod-shaped and pink-colored. The Oxalicibacterium species, strain 16B04G was short rod-shaped and pink-colored. The Sphingomonas species, strain 16B01D was short rod-shaped and yellow-colored. The Variovorax species, strain 17U4-2 was cocci-shaped and yellow-colored. The Paracoccus species, 17J28-10 was cocci-shaped and orange-colored. Phylogenetic analysis based on 16S rRNA gene sequence showed that strains 17SD2_15, 17Sr1_23, 17SD2_13, 17Sr1_31, 17gy_18, 16B15D, 16B02D, 16B04G, 16B01D, 17U4-2 and 17J28-10 were most closely related to Belnapia soli (with 99.9% similarity), Methylobacterium gregans (99.1%), Methylobacterium isbiliense (99.6%), Methylobacterium oxalidis (99.9%), Microvirga aerilata (98.7%), Methylobacterium aerolatum (99.0%), Microvirga vignae (100.0%), Noviherbaspirillum canariense (100.0%), Sphingomonas desiccabilis (100.0%), Variovorax humicola (99.6%), and Paracoccus acridae (99.1%), respectively. This is the first report of these eleven species in Korea.

Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen

  • Jeon, Ji-Yeong;Jung, Kyung-Min;Chang, Jun;Choi, Eun-Young
    • IMMUNE NETWORK
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    • v.11 no.2
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    • pp.100-106
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    • 2011
  • Background: Disparities of Minor H antigens can induce graft rejection after MHC-matched transplantation. H60 has been characterized as a dominant antigen expressed on hematopoietic cells and considered to be an ideal model antigen for study on graft-versus-leukemia effect. Methods: Splenocytes from C57BL/6 mice immunized with H60 congenic splenocytes were used for establishment of H60-specific CTL clones. Then the clones were characterized for proliferation capacity and cytotoxicity after stimulation with H60. Clone #14, #15, and #23 were tested for the TCR binding avidity to H60-peptide/$H-2K^b$ and analyzed for TCR sequences. Results: H60-specific CTL clones showed different levels of proliferation capacity and cytotoxic activity to H60-stimulation. Clones #14, #15, and #23 showed high proliferation activity, high cytotoxicity, and low activities on both aspects, respectively, and have TCRs with different binding avidities to H60-peptide/$H-2K^b$ with $t_{1/2}$ values of 4.87, 6.92, and 13.03 minutes, respectively. The TCR usages were $V{\alpha}12D-3-01+J{\alpha}11-01$ and $V{\beta}12-1-01+D{\beta}1-01+J2-7-01$ for clone #14, $V{\alpha}13D-1-02+J{\alpha}34-02$ and $V{\beta}13-1-02+D{\beta}2-01+J{\beta}2-7-01$ for clone #15, and $V{\alpha}16D+J{\alpha}45-01$ and $V{\beta}12-1-01+D{\beta}1-01+J{\beta}2-5-01$ for clone #23. Conclusion: The results will be useful for modeling GVL and generation TCR transgenic mouse.

The study on relation between ocular function and accommodative facility (시기능과 조절 용이성과의 관계 연구)

  • Park, Hyun-Ju
    • Journal of Korean Ophthalmic Optics Society
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    • v.10 no.4
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    • pp.375-380
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    • 2005
  • To assess convergence and accommodation at a short distance, 92 selected subjects without any ocular diseases by apperatuses of visual chart(Shinnippon CT30) and phoropter(Shinnippon VT 10) at a short distance(40 cm) were tested upon MEM retinoscopy(Welch Allyn, USA). BELL retinoscopy(Welch Allyn, USA), binocular accommodative facility (${\pm}2.00$ D flipper. Bernell Co., USA), vergence facility(prism flipper, Bernell Co., USA). There were such test results as MEM retinoscopy(R/L)($+1.05{\pm}0.87/1.02{\pm}0.80$ D). BELL retinoscopy ($17.89{\pm}12.12/17.94{\pm}11.72cm$), binocular accommodative facility (R/G)($7.69{\pm}6.48cpm$). binocular accommodative facility(polaroid)($11.76{\pm}5.22cpm$), monocular accommodative facility(R/L) ($13.9{\pm}6.27/13.8{\pm}5.96cpm$), vergence facility($13.5{\pm}6.40cpm$).

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Estimates of Parameters for Genetic Relationship between Reproductive Performances and Body Condition Score of Hanwoo Cows

  • Choi, S.B.;Lee, J.W.;Choy, Y.H.;Na, K.J.;Kim, N.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.7
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    • pp.909-914
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    • 2005
  • This study was conducted to estimate phenotypic and genetic parameters of body condition score (BCS) and reproductive traits in Hanwoo cows. DFREML procedures were applied to obtain variance-covariance components and heritability estimates with single or two-trait models. Estimates of phenotypic correlations of BCS at service with BCS at calving was 0.16 and 0.26 with calving interval, 0.08 with gestation length, and 0.06 with number of services per conception, respectively. Estimates of phenotypic correlation of BCS at calving was 0.10 with calving interval, 0.13 with gestation length, and 0.10 with number of services per conception, respectively. Estimates of phenotypic correlation were low and negative, -0.11 between calving interval and gestation length and -0.13 between gestation length and number of services per conception. Estimates of direct genetic correlation were -0.06, between BCS at service and BCS at calving, 0.37 between BCS at service and BCS at weaning, and -0.18 between BCS at calving and BCS at weaning. Estimates of direct genetic correlation of days from calving to the 1st service were 0.17 with number of services per conception and -0.21 with BCS at service. Estimates of direct genetic correlation for BCS at calving were -0.02 with number of services per conception and -0.08 with BCS at service. Estimates of direct genetic correlation for BCS at weaning were 0.02 with number of services per conception and -0.07 with BCS at service. Estimates of direct heritability from single trait analyses were 0.13 for BCS at service, 0.20 for BCS at calving, 0.02 for BCS at weaning, and 0.20 for number of service per conception, respectively. Estimates of direct heritability were 0.20 for birth weight and 0.10 for weaning weight.

Chemical Composition and Acaricidal Effects of Essential Oils Extracted from Ligustrum japonicum against Acaridae and Pyroglyphid Mites

  • Lee, Hoi-Seon
    • Journal of Applied Biological Chemistry
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    • v.58 no.3
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    • pp.197-199
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    • 2015
  • The composition of the essential oil of Ligustrum japonicum leaves was determined by GC-MS analysis. The major constituents of L. japonicum leaf oil were germacrene D (40.50%), ${\alpha}$-pinene (13.63%), (-)-${\beta}$-elemene (6.42%), ${\beta}$-caryophyllene (5.73%), and $\delta$-cadinene (5.47%). The acaricidal activities of L. japonicum oil were evaluated against acaridae and pyroglyphid mites. In the fumigant bioassay, the $LD_{50}$ values of L. japonicum oil were 16.48, 12.38, and $15.63{\mu}g/cm^3$ against Tyrophagus putrescentiae, Dermatophagoides farinae, and D. pteronyssinus, respectively. In the contact bioassay, the $LD_{50}$ values of L. japonicum oil were 8.02, 5.02, and $7.67{\mu}g/cm^2$ against T. putrescentiae, D. farinae, and D. pteronyssinus, respectively.

13 weeks repeated oral dose toxicity studies with LMK02-Jangwonhwan in SD rats (LMK02의 Sprague-Dawley 랫드를 이용한 13 주간 반복 경구투여 독성시험)

  • Kang, Hyung-Won;Jang, Hyun-Ho;Park, Jang-Ho;Kim, Tae-Heon;Lyu, Yeoung-Su
    • Journal of Oriental Neuropsychiatry
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    • v.23 no.2
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    • pp.99-120
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    • 2012
  • Objectives : The oriental medicine Jangwonhwan, a boiled extract of 12 medicinal herbs/mushrooms, has been prescribed to patients with cognitive dysfunction, as originally described in the Korean medical text, DonguiBogam(amnesia chapter). Recently, a modified formula of Jangwonhwan (LMK02-Jangwonhwan) consisting of seven medicinal plants/mushrooms, was shown to reduce the ${\beta}$-amyloid deposition in the brain of Tg-APPswe/PS1dE9 mouse model for Alzheimer's disease. The toxicity of LMK02-Jangwonhwan was investigated in SD rats, by a daily oral administration for 13 weeks and NOAEL(No observed adverse effect dose), a definite toxic dose and target organ, as well. Methods : Quality control of the tablet form of LMK02-Jangwonhwan was established by estimating the indicative components, Ginsenoside Rg3 of Red Ginseng and Decursin of Angelicagigas Nakai. The toxicity of LMK02-Jangwonhwan was investigated in 6 week old, specific pathogen free (SPF), Sprageu-Dawley rats by oral administration. Each test group consisted of 10 male and 10 female rats. The groups received doses of 500, 1,000 or 2,000 mg/kg/day of test substance for 13 weeks. The clinical signs, death rate, body weight, food consumption, ophthalmic examination, urinalysis, hematological and serum biochemistry, organ weight and pathological changes were examined and compared with those of the control group. Results : The 13-week repeated oral treatment doses didn't result in any specific symptoms or death. There were no significant changes in the rat's weight and food consumption. Further, ophthalmic examination, urinalysis, hematological, serum biochemistry test and organ weight revealed no significant differences. Conclusions : The no-observed-adverse-effect level(NOAEL) of LMK02 for male and female Sprague-Dawley rats was determined as 2,000mg/kg/day and the target organ wasn't confirmed. Because no significant adverse effects were observed, the target organ could not be determined.

A study of refraction state of middle aged & manhood in Daegu (대구지역 중·장년층의 굴절상태 연구)

  • Choi, Gei-Hun
    • Journal of Korean Ophthalmic Optics Society
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    • v.9 no.2
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    • pp.323-332
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    • 2004
  • This study researched the visual acuity test object and Auto-refractormeter, visual of near power. The object were composed of middle aged, the old men and women who in habit Daegu. The results were as follows : 1. The subjects consisted of 537 people, 29.98% men, 70.02% women. 2. The emmetropia was 1.12% for myopia, 2.79% for hyperopia, 96.09% for astigmatism. 3. The abnormal refraction was composition for myopic compound astigmatism(16.57%), hyperopia compound astigmatism(45.62%), Mixed astigmatism(33.89%). 4. On the Myopic Spherical Equivalent(S.E) power, the range of -0.50D ${\leq}$ M.S.E < -1.00D was 21.67%, -1.00D ${\leq}$ M.S.E < -2.00D was 48.89%, -2.00D ${\leq}$ M.S.E < -6.00D was 29.44%. 5. On the Hyperopic Spherical Equivalent(S.E) power, the range of +0.50D ${\leq}$ H.S.E < +1.00D was 28.57%, +1.00D ${\leq}$ H.S.E < +2.00D was 49.30%, +2.00D ${\leq}$ H.S.E < +6.00D was 23.13%. 6. The addition power was 1.00D(8.01%), 1.50D(8.57%), 2.00D(13.78%), 2.50D(16.57%), 3.00D(16.95%), 3.50D(17.88%), 4.00D(18.25%).

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Comparison of Accuracy for Autorefraction according to Measuring methods (측정방식에 따른 자동굴절검사의 정확도 비교)

  • Jeong, Youn Hong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.8
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    • pp.353-359
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    • 2018
  • In this study, the performance between subjective refraction and open-field/closed view autorefraction was estimated. We measured the refractive error of early adults aged 18 to 20 years who did not have eye disease. The differences between measurements obtained by subjective refraction and open-field autorefraction for SE, J0, and J45 were $-0.13{\pm}0.53D$ (p=0.17), $+0.33{\pm}0.68D$ (p=0.01), and $+0.13{\pm}0.68D$ (p=0.26), respectively, with only J0 differing significantly. The differences between the measurements of subjective refraction and closed-view autorefraction for SE, J0, and J45 were $-0.30{\pm}0.42D$ (p=0.00), $+0.30{\pm}0.71D$ (p=0.02), and $-0.02{\pm}0.63D$ (p=0.88), respectively, with only SE and J0 differing significantly. The coefficient of accuracy for SE, J0, and J45 components of open-field and closed-view autorefraction were 1.04, 1.33, and 1.34 and 0.83, 1.40, and 1.24, respectively. It is possible to predict the refractive error, which is necessary when deciding on subjective refraction, by measuring the objective refraction of open-field/closed view autorefractors.

Difference of Developmental Time, Survival Rate and Sex Ratio of Dichocrocis punctiferalis (Lepidoptera: Pyralidae) on Three Hosts

  • Choi, Kwang Sik;Han, Kyung Sik;Park, Il Kwan;Hong, Jeong Im;Kim, Chul Soo;Chung, Yeong Jin;Shin, Sang Chul
    • Journal of Korean Society of Forest Science
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    • v.95 no.2
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    • pp.174-176
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    • 2006
  • The experiments were conducted by supplying hosts with natural food(chestnut, peach, Quince). The developmental difference of peach pyralid moth, Dichocrocis punctiferalis was examined in the laboratory under three different natural food regime. The periods of egg, larva and pupa were $6.01{\pm}0.07$, $12.23{\pm}0.03$ and $13.32{\pm}0.01$ days on the chestnut fruit, $6.21{\pm}0.01$, $18.69{\pm}0.02$ and $13.38{\pm}0.03$ days on the peach fruit and $7.02{\pm}0.04$, $22.62{\pm}0.04$ and $13.44{\pm}0.14$ days on the quince fruit, respectively. The growth of D. punctiferalis larva was better chestnut fruit than other tested fruits. The rates(%) of hatching, pupation and emergence were 94.0, 57.0 and 63.3 on the chestnut fruit, 89.2, 77.8 and 85.7 on the peach fruit and 79.6, 52.6 and 70.7 on the quince fruit, respectively. The survival rate(%) of D. punctiferalis from hatching to emergence were 31.0 on the chestnut fruit, 4.8 on the peach fruit and 14.3 on the quince fruit, respectively. The sex ratio (female: male) of all pupae obtained on the tested natural food fruits were 52.7 : 47.3. The sex ratio of D. punctiferalis reared on three difference food fruits were no significantly. It can be used a as the basic research for the study of D. punctiferalis.

Association between Vitamin D Level in Blood and Periodontitis in Korean Elderly

  • Yoon, Na-Na;Lee, Ji-Young;Yu, Byeng-Chul
    • Journal of dental hygiene science
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    • v.17 no.3
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    • pp.233-241
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    • 2017
  • This study identified an effective control method for periodontitis by investigating the association between blood levels of vitamin D and periodontitis in Korean elderly based on raw data from the fifth Korea National Health & Nutrition Examination Survey of 2010 (KNHANES). In this study, 1,021 adults over 65 years of age were evaluated based on data from the KNHANES. Periodontal disease was assessed using community periodontal index (CPI), with CPI codes ${\geq}3$ defined as periodontitis. Blood levels of vitamin D were measured from blood samples and divided into four groups (first quartile: ${\leq}13.23ng/ml$, second quartile: 13.24~16.95 ng/ml, third quartile: 16.96~21.58 ng/ml), and fourth quartile >21.59 ng/ml). Using multiple logistic regression analyses, the variables were adjusted for general characteristics, oral health-related characteristics, health-related characteristics, and bone mineral density. The statistical analysis was performed using the SAS (ver. 9.2). The results of this study are as follows: the prevalence of periodontitis was 42.6% in Korean elderly. After adjusting for general, oral health-related, and health-related, the risk of periodontitis in the first quartile group was 1.74 times (95% confidence interval [CI], 1.02~2.98) higher than that of the fourth quartile group (p=0.041). After adjusting for general, oral health-related, and health-related characteristics as well as bone mineral density, the risk of periodontitis in the first quartile group was 1.73 times (95% CI, 1.02~2.96) higher than that of the four quartile group (p=0.042). There was a significant relationship between blood vitamin D level and periodontitis in Korean elderly. For the prevention of periodontitis, factors related to vitamin D should be considered along with other risk factors.