• Title, Summary, Keyword: 항산화물질

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Optimization of Extraction Conditions of Antioxidants from Sporophyll of Undaria pinnatifida by Response Surface Methodology (반응표면분석법에 의한 미역 포자엽의 항산화물질 추출 조건 최적화)

  • Yu, Mi-Yeong;Kim, Gyeong-Hwan;Lee, Jae-U;Kim, Sang-Gwon;Yang, Ji-Yeong
    • Microbiology and Biotechnology Letters
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    • v.32 no.4
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    • pp.317-321
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    • 2004
  • We selected a solvent for producing antioxidants from sporophyll of Undaria pinnatOptimum extraction condition for antioxidant ability(Y) of sporophyll of U. pinnatifida was investigated using response surface methodology(RSM). A compound central design was used with variables ($X_1$)temperature($^{\circ}C$) (3.4; 20; 60; 100; 116.6); ($X_2$) pH(1.8; 3; 6; 9; 10.2) and ($X_3$) treatment time(min) (1.7; 10; 30; 50; 58.3). Antioxidant activities were increased with decreasing pH and increasing temperature. The optimal extraction conditions for antioxidant ability(Y) of sporophyll of U. pinnatifida were found to be temperature $51.55^{\circ}C$, pH 4.2 and treatment time 28.2 min. Among the variables tested, pH showed greater significant facin extraction of antioxidants from sporophyll of U. pinnatifida.

Antioxidant Activity of Substances Extracted by Alcohol from Chungkookjang Powder (분말청국장에서 알코올로 추출한 물질의 항상화능)

  • 이재중;조창훈;김지연;이동석;김한복
    • Korean Journal of Microbiology
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    • v.37 no.3
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    • pp.177-181
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    • 2001
  • It is previously reported that Bacillus licheniformis B1 strain isolated from nature was successfully used for Chungkookjang fermentation. Antioxidant activity of its powder was determined in this study. Sephadex G-75 gel filtration chromatography was performed, far soluble fractions of the powder extracted by distilled water. The soluble fractions were separated into large and small fractions. The substance 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used as an electron acceptor. Antioxidant activity was found in the small fractions. Five% solution of the Chungkookjang powder was the most effective in the extraction of antioxidant substances from the powder. It was proven in this study that strong antioxidant activity still remained in the Chungkookjang powder.

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Antioxidant Activity and Characterization of Exiguobacterium sp. SC2-1 Isolated from Sea Water (해양에서 분리한 Exiguobacterium sp. SC2-1의 항산화 활성 및 특성)

  • Kim Man-Chul;Park Guen- Tae;Son Hong-Joo;Choi WooBong;Heo Moon-Soo
    • Korean Journal of Microbiology
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    • v.41 no.1
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    • pp.74-80
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    • 2005
  • For the reseach of the natural marine antioxidant, an antioxidant-producing bacterium was isolated from seawater in Jeju costal area. The isolated strain SC2-1 was Gram-positive, catalase positive, oxidase negative, motile and small rods. The strain utilized sucrose, dextrose, fructose, mannitol and maltose as a sole carbon and energy source and NaCl required for growth. The radical scavenging activity of the culture supernatant was detemined by DPPH method. This bacterium was identified based on morphological, biochemical characteristics and 16S rDNA sequence analysis, and then was named Exiguobacterium sp. SC2-1. The optimum conditions of culture for production antioxidnat were $25^{\circ}C$, pH 6-8 and $4\%$ NaCl. The stain showed the highest activity and growth cultured in medium which added $1\%$ maltose. Hydroxyl radical activity of the supernatant of Exiguobacterium sp. SC2-1 was $73\%$. The SOD activity of the culture supernatant was estimated about $35\%$.

Studies on the Antioxygenic Substances in Panax ginseng Roots -I. The Antioxidative Action of Various Solvent Extracts of Panax ginseng Roots- (인삼중의 항산화물질에 관한 연구 -제 1 보 : 인삼의 각종 용매 추출물의 항산화작용-)

  • Paik, Tai-Hong;Hong, Jeong-Tai;Hong, Soon-Yoog
    • Korean Journal of Food Science and Technology
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    • v.14 no.2
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    • pp.130-135
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    • 1982
  • The antioxidative action of petroleum ether, chloroform, chloroform-methamol (2 : 1, v/v), methanol and methanol-water extracts of Panax ginseng roots in the autoxidation of mixed methyl esters of unsaturated fatty acids (MEUFA) was investigated in vitro. All of the extracts of Panax ginseng roots possessed antioxidative activity and inhibited the formation of hydroperoxides in the autoxidation of mixed methyl esters of unsaturated fatty acids (MEUEA). The induction period in the autoxidation of MEUFA was extended by the addition of each extract. The antioxidative activity was more prominent with chloroform and chloroform-methanol (2 : 1, v/v) extracts than with other extracts. The antioxidative activity of each extract estimated by Olcott's oven test and the measurement of peroxide value (POV) showed a similar tendency. From the results obtained, it was conclude that extracts of Panax ginseng roots had remarkable antioxidative activity in the autoxidation of MEUFA in vitro.

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Isolation, Identification, and Activity of Rosmarinic Acid, a Potent Antioxidant Extracted from Korean Agastache rugosa (한국산 방아잎(배초향, Agastache rugosa O. Kuntze)에서 항산화물질 로즈마린산의 분리, 동정 및 활성)

  • Kim, Jong-Bum;Cho, Kang-Jin;Hwang, Young-Soo;Park, Ro-Dong;Kim, Jung-Bong
    • Applied Biological Chemistry
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    • v.42 no.3
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    • pp.262-266
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    • 1999
  • Thirty plants were screened for their antioxidative activity. The extracts of Agastache rugosa, Schizonepeta tenuifolia and Lycopus lucidus had high free radical(2,2-diphenyl-1-picrylhydrazyl) scavenging activities. Methanol extract of Agastache rugosa was fractionated with hexane, chloroform, ethyl acetate, and water. The ethyl acetate fraction showed the highest antioxidant activity in the DPPH test. The ethyl acetate fraction was applied to Sephadex LH-20 column, and the fractions showing antioxidative activity were collected and used for identification of the substance. The purified substance was applied to mass, IR, UV and NMR spectroscopy. The spectra of mass, IR, UV and NMR implied that the substance is a rosmarinic acids as a kind of phenolic compound. The rosmarinic acid has more antioxidative effect than those of BHT and ${\alpha}-tocopherol$ in the Rancimat test.

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